Factors affecting enzyme function: pH, temperature leading to denaturing
Importance of active site residues (acidic/basic)
Concept of feedback inhibition
Enzyme three-dimensional shape affects function
Role of reactants/products in enzymatic reactions
Understanding allosteric inhibition and its implications
Substrate: molecule the enzyme acts upon
Active Site: specific area of enzyme where substrates bind
Competitive Inhibition: similar-shaped molecules block substrate binding
Glycolysis: occurs in the cytoplasm, breaks down glucose, requiring initial energy input
Pyruvate Oxidation: occurs in mitochondria, converts pyruvate into Acetyl-CoA
Citric Acid Cycle: occurs in mitochondria, processes Acetyl-CoA to produce electron carriers
Electron Transport Chain: crucial for ATP production using a hydrogen gradient
ATP Synthase: enzyme that produces ATP in the Electron Transport Chain
Oxidation: losing electrons
Reduction: gaining electrons
Key electron carriers: NAD and FAD (NADH, FADH2)
Understand energy movements and bonds in metabolic reactions
The Calvin Cycle: converts carbon dioxide into glucose via carbon fixation
Inputs: CO2, ATP, NADPH
Requires RUBP and enzyme Rubisco
Convert solar energy into chemical energy, producing ATP and NADPH to fuel the Calvin Cycle
Understand relationships between photosynthesis and cellular respiration in the context of energy conversion and the cyclical nature of these processes.Analyze diagrams and experimental data to answer questions effectively.
Familiarity with key terms (delta G, energy types) and metabolic pathways is essential for exam readiness.