Cells

Types of Cells

  1. Animal Cell

    • Does not have a cell wall or chloroplast

    • Small vacuole

  2. Plant Cell

    • Contains a cell wall

    • Chloroplast and large vacuole

  3. Bacteria Cell

    • Prokaryotic, contains no nucleus

    • No membrane-bound organelles

Cell Categories

  1. Prokaryotic Cells

    • Lacks a membrane-enclosed nucleus

    • No membrane-enclosed organelles

    • Found only in the domains Bacteria and Archaea

  2. Eukaryotic Cells

    • Contains a nucleus

    • Other organelles are bound by membranes

Key Cell Organelles and Structures

  1. Mitochondria

    • Powerhouse of the cell

    • Site of ATP (energy) production

  2. Cell Membrane

    • Thin, flexible barrier around a cell

    • Regulates what enters and leaves the cell

  3. Nucleus

    • Large membrane-enclosed structure

    • Contains the cell's genetic material in the form of DNA

  4. Nuclear Membrane

    • Highly-porous membrane

    • Separates the nucleus from the cytoplasm

  5. Nucleolus

    • Found inside the nucleus

    • Produces ribosomes

  6. Lysosomes (Animal Cell)

    • Filled with enzymes needed to break down certain materials

  7. Ribosomes

    • Makes proteins

  8. Vacuole

    • Stores materials such as water, salts, proteins, and carbohydrates

  9. Endoplasmic Reticulum (ER)

    • Extensive membranous network

    • Composed of ribosome-studded (rough) and ribosome-free (smooth) regions

  10. Golgi Apparatus

    • Modifies and packages proteins for export by the cell

  11. Cytoplasm

    • Jelly-like fluid inside the cell

    • Organelles are suspended in it

  12. Cytoskeleton

    • Network of fibers that holds the cell together

    • Helps the cell to keep its shape and aids in movement

  13. Nucleoid (Prokaryotic Cell)

    • Non-membrane-bounded region

    • Where the DNA is concentrated

  14. Plasmid

    • Small ring of DNA

    • Carries accessory genes separate from the bacterial chromosome

  15. Plastid (Plant Cell)

    • Group of plant organelles

    • Used for storage of starches, lipids, or pigments

  16. Chloroplast (Plant Cell)

    • Site where photosynthesis occurs

  17. Cell Wall (Plant Cell)

    • Rigid layer of nonliving material

    • Surrounds the cells of plants and some other organisms

  18. Capsule (Bacteria)

    • Sticky layer that surrounds the cell walls of some bacteria

    • Protects the cell surface and sometimes helps to glue the cell to surfaces

  19. Pilus (Bacteria)

    • Short, thick hair-like protein structure

    • Allows a bacterium to attach to other bacteria and surfaces

  20. Flagellum

    • Long, hairlike structure

    • Enables the cell to move

Cell Processes

  1. Mitosis

    • Part of eukaryotic cell division

    • Cell nucleus divides

  2. Meiosis

    • Cell division that produces reproductive cells in sexually reproducing organisms

  3. Homeostasis

    • Tendency to maintain a balanced or constant internal state

    • Regulation of any aspect of body chemistry, such as blood glucose, around a particular level

Cell Theory

  • All living things are composed of cells

  • Cells are the basic units of structure and function in living things

  • New cells are produced from existing cells

Example Experiments

  1. Observing Onion Cells under a Microscope

    • Objective: To identify plant cell structures such as the cell wall, nucleus, and vacuole.

    • Materials: Onion, microscope, slides, iodine solution, and coverslip.

    • Procedure: Peel a thin layer of onion skin, place it on a slide, add a drop of iodine solution, cover with a coverslip, and observe under a microscope.

  2. Effect of Different Solutions on Plant Cells

    • Objective: To observe osmosis in plant cells.

    • Materials: Potato, sugar solution, distilled water, and microscope.

    • Procedure: Cut potato slices, place them in different concentrations of sugar solutions and distilled water, observe changes in cells under a microscope.

  3. Bacteria Cultures and Antibiotic Effect

    • Objective: To observe the growth of bacteria and the effect of antibiotics.

    • Materials: Agar plates, bacteria samples, antibiotic discs, incubator.

    • Procedure: Spread bacteria on agar plates, place antibiotic discs on the agar, incubate and observe the zones of inhibition around antibiotic discs.

Redi's experiment, conducted by the Italian scientist Francesco Redi in the 17th century, was a landmark study in the field of biology that aimed to disprove the theory of spontaneous generation. This theory suggested that living organisms could arise from non-living matter.

In his experiment, Redi used jars containing meat. He divided the jars into three groups:

  1. Open jars with meat - these jars were exposed to the air.

  2. Sealed jars with meat - these jars were completely sealed to prevent any air from entering.

  3. Jars covered with gauze - these jars were covered with gauze that allowed air in but prevented flies from laying eggs on the meat.

Over time, Redi observed that only the open jars produced maggots, while the sealed jars and gauze-covered jars did not. This led him to conclude that the maggots arose not spontaneously from the meat, but from fly eggs that were present in the environment. Redi's experiment provided strong evidence against spontaneous generation and laid the groundwork for further studies in microbiology and the understanding of biogenesis.

Pasteur's Experiment (1857): Louis Pasteur conducted a pivotal experiment that further disproved the theory of spontaneous generation and established the principles of microbiology.

Objective: To show that microorganisms in the air cause spoilage and fermentation.

Materials:

  • Broth in S-shaped flasks

  • Heat source

Procedure:

  1. Pasteur used S-shaped flasks to allow air to enter but prevent dust and microorganisms from contaminating the broth.

  2. He boiled the broth to sterilize it and kill existing microorganisms.

  3. The flasks were left exposed to air without any additional covering.

Results:

  • The broth in the S-shaped flasks remained clear and free of microbial growth for a long time, while broth in open flasks became cloudy with microbial growth.

  • The design of the S-shaped neck trapped airborne bacteria, preventing them from reaching the broth.

Conclusion: Pasteur concluded that microorganisms in the air contaminated the broth, thus supporting the idea that life does not spontaneously arise from non-living matter, but is instead introduced from the environment. This experiment solidified the germ theory of disease and established sterilization techniques in microbiology.

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