Principles of Disease and Epidemiology – Vocabulary Review

Pathology, Infection, and Disease

Pathology – study of disease itself; seeks to understand structural and functional changes produced by disease.
Etiology – the precise cause of a disease (infectious agent, genetic defect, toxin, etc.).
Pathogenesis – mechanisms by which disease develops and progresses.
Infection – invasion or colonisation of a host by pathogenic organisms.
Infectious disease – any deviation from a healthy state that is produced by an infection.

Human Microbiome

• Establishes in utero – placental microbiome has Enterobacteriaceae & Propionibacterium.
Birth canal – Lactobacilli transferred to neonate’s intestine during vaginal delivery.
• Additional colonisation from food, people, pets; communities fluctuate through life.

Types of Microbiota

Normal (resident) microbiota – permanently inhabit the host; usually harmless under normal conditions.
Transient microbiota – present days-months, then disappear.

Factors Shaping Microbiota

• Nutrient availability; temperature, pH, salinity, O2; mechanical forces (peristalsis, flow of saliva, urine); host defenses (lysozyme, IgA, complement).
• Host attributes: age, diet, nutritional state, geography, hygiene, housing, occupation, lifestyle.
• Crucial in immune-system maturation and modulation.

Symbiosis & Host Relationships

Symbiosis – any close relationship between two organisms in which at least one depends on the other.
Commensalism – one benefits, other unaffected (e.g. Staphylococcus epidermidis on skin).
Mutualism – both benefit (e.g. E. coliE.\ coli synthesising vitamins K/B in colon while obtaining nutrients).
Parasitism – one benefits at expense of host (influenza virus, helminths, protozoa, etc.).
Opportunistic pathogens – normal/resident organisms that cause disease when ecological niche changes (immune suppression, altered site, etc.).

Microbial Antagonism (Competitive Exclusion)

• Normal flora suppress overgrowth of pathogens by:
– Competing for nutrients/space.
– Producing bacteriocins, acids, peroxides.
– Modifying local pH & O2.
• Classic examples: vaginal Lactobacillus lowers pH preventing Candida; gut flora outcompete Salmonella & C. difficile.

Koch’s Postulates

  1. Same pathogen present in every case.

  2. Pathogen isolated & grown in pure culture.

  3. Culture must cause same disease in healthy, susceptible animal.

  4. Pathogen re-isolated from inoculated animal & confirmed identical.
    • Prove causation; foundation of medical microbiology.
    Exceptions – polymicrobial diseases, microbes only in humans (HIV), non-cultivable agents (Treponema pallidum, viruses), different pathogens with same syndrome (pneumonia).

Classifying Infectious Diseases

Symptoms – subjective, felt by patient (pain, malaise).
Signs – objective, measurable/observable (fever, rash, WBC count).
Syndrome – specific constellation of signs + symptoms.

Communicability

Communicable disease – spreads host-to-host (TB, measles).
Contagious disease – easily & rapidly spread (influenza, chickenpox).
Noncommunicable disease – not spread host-to-host (tetanus, diabetic ketoacidosis).

Occurrence Metrics

Incidence – new cases/time period.
Prevalence – total existing cases at given time (includes old + new).

Frequency Categories

Sporadic – occasional (typhoid in US).
Endemic – constantly present (common cold).
Epidemic – many cases in short time (2014 Ebola in W. Africa).
Pandemic – global epidemic (COVID-19, 1918 influenza).

Severity / Duration

Acute – rapid onset, short duration (influenza).
Chronic – slow onset, long duration (TB, hepatitis B).
Subacute – intermediate (subacute sclerosing panencephalitis).
Latent – dormant agent becomes active (shingles from varicella-zoster).
Duration – avg. time from diagnosis to cure/death.
Herd immunity – majority immune → limits spread; vaccination goal.

COVID-19 Severity Scale Example

• Asymptomatic, Mild, Moderate, Severe (pneumonia), Critical (SARS, ARDS, sepsis).

Extent of Host Involvement

• **Local