Good Laboratory Practices-2

Centro Escolar University - Makati School of Science and Technology

  • Biological Sciences Department

  • Presentation by: Montuya, John Gilbert A.

  • Module 2: Good Laboratory Practices

Table of Contents

  • General Laboratory Use Guidelines

  • Personal Protective Equipment

  • Good Laboratory Practices

  • Laboratory Waste Management

Section A

Coverage of Laboratory Manual

Borrowing and Returning of Materials

Incident Reports

Section B

Personal Protective Equipment (PPE)

Good Laboratory Practices (GLP)

Cleaning Materials and Lockers

Laboratory Waste Disposal Protocols

Accord of Understanding

Qualified Users of Laboratory

  • Laboratory Office Personnel

  • Faculty handling laboratory subjects

  • Students that are currently enrolled in laboratory subjects

Borrowing of Materials

  1. Present valid Laboratory ID or Certificate of Registration

  2. Fill out the Borrower’s Slip Form (in-campus)

  3. Check the condition of borrowed materials.

  4. Place a signature beside your name to accept the materials.

Returning of Materials

  1. Return the materials in complete and good condition.

Incident Reports

For accidents, contamination, and damages:

  1. File an incident report within 72 hours of incidence.

  2. Involved students and faculty should sign the incident report.

Personal Protective Equipment

  • Definition: A safety outfit that minimizes any possible hazard to an individual.

  • Personal: Only effective on the wearer.

  • Protective: Minimizes contact and transmission.

  • Equipment: Contains multiple components.

Specific Components of Laboratory PPE

  • Skin Protection

  • Eye Protection

  • Oronasal Protection

  • Hair Protection

Skin Protection

  • Purpose: Prevents direct skin contact with laboratory materials.

  • Components:

  • Laboratory Gowns

  • Laboratory Hairnets

  • Laboratory Gloves

  • Laboratory Shoes (optional)

  • Laboratory Goggles (optional)

Laboratory Gowns

  • Types:

  • Multiple Use

  • Single Use

Laboratory Gloves

  • Essential for minimizing exposure to hazardous materials.

Laboratory Goggles

  • Purpose: Prevent exposure from chemical fumes, splashes and spillage.

Oronasal Protection

  • Purpose: PPE for Nose and Mouth to prevent ingestion of solids and minimize aerosol expulsion.

  • Laboratory Masks

Good Laboratory Practices

  • Protocols to maximize safety and minimize hazard inside the laboratory:

  • Notice prohibiting food, drinks, cell phones, and the requirement for lab coats and safety glasses.

  • Emergency facilities including first aid kits, fire extinguishers, and eye wash stations.

NFPA Hazard Diamond

  • Health Hazard: Levels 0-4 indicating severity of health risks.

  • Fire Hazard: Levels 0-4 indicating flammability.

  • Specific Hazard: Potential risks associated with chemical properties.

GHS Hazard Pictogram Symbols

  • Corrosive: Causes severe burns and eye damage.

  • Flammable: Warning for flammable substances.

  • Poisonous: Toxic substances that can be fatal if ingested or inhaled.

  • Explosive: Indicates self-reactive substances that may explode when heated.

  • Oxidizing: Gases, solids, and liquids that can exacerbate fire hazards.

  • Health Hazard: Serious health consequences include carcinogenic effects and inhalation risks.

  • Environmental Hazard: Chemicals harmful to the environment.

GHS Laboratory Waste Disposal

  • **Types of Waste: **:

  • Non-infected waste

  • Infected waste

  • Anatomical waste

  • Sharp waste

  • Radioactive waste (recyclable)

Laboratory Cleaning Materials (Group)

  • Required items for cleaning purposes:

  • Cleaning Bin

  • Sterile single-use PPE materials

  • Liquid Soap

  • Tissues or Paper Towels

  • Dishwashing Sponge and Small Bottle Brush

  • 1 pc - 70% Alcohol (ethanol or isopropyl) – 500 mL

  • 3 pcs - multi-use cloth rags

  • 1 pc – Empty Bottle Container (200 mL)

Laboratory Archival Materials (Group)

  • Items to properly label and preserve laboratory products:

  • Distilled Water (500 mL)

  • Nail Polish (clear)

  • Masking Tape (2” size)

  • Glass slides with coverslip

  • Reusable plastic droppers

Activity: Chromosome Observation

  • Required materials for the laboratory activity:

  • Onion Root Tip (1 cm each) – at least 10 pcs

  • Preparation involves placing onion bulbs in water for growth.