Aseptic Techniques in Biotechnology

Aseptic techniques prevent contamination. Key principles:

Sterilization: Eliminating microorganisms.

Disinfection: Reducing microbes on surfaces.

Sterile environment: Using laminar flow hoods.

• Hygiene: Washing hands, wearing lab coats and gloves.

• Sterile handling: Using sterile instruments.

Importance:

• Cell Culture: Prevents contamination.

• Microbiology: Ensures pure cultures.

• Recombinant DNA Technology: Maintains DNA purity.

• Pharmaceutical Production: Ensures biopharmaceuticals are free from contamination.

• Food Biotechnology: Prevents spoilage.

Examples:

• Laminar flow hoods: Sterile workspace.

• Sterilizing equipment: Autoclaving glassware.

• Disinfecting surfaces: Wiping with ethanol.

• Handwashing: Thoroughly washing hands.

• PPE: Using lab coats, gloves, and masks.

• Flame sterilization: Passing container necks through flame.