AP BIO EXAM REVIEW

UNIT 1 - Chemistry of Life

  • Hydrogen Bonds in Water

    • Properties of water

      • Water dissolves polar and charged molecules

      • Water is cohesive and adhesive

      • Polar because bent shape and partial negative oxygen/partially positive hydrogen

      • Water has high heat capacity - it takes a lot of energy to raise the temperature

      • Water has a high heat of vaporization - water can be used to cool people off and converted to steam

      • Molecules in ice are further apart which is why it is less dense than liquid water

    • Water attracts to each other using hydrogen bonds

      • Attracts to other polar molecules and ions

      • Hydrophilic - molecules attracted to water

      • Hydrophobic - molecules not attracted to water

  • Cohesion and adhesion of water

    • Cohesion - attraction between molecules of the same kind

      • Cohesive forces cause surface tension 

      • Allows water to support itself and create droplets because it's surrounded by air so it creates stronger bonds to itself

    • Adhesion - attraction between molecules of different kinds

      • Water is especially adhesive with molecules with a positive or negative charge

      • Water can “climb” through glass tubes - called capillary action - because the water is adhesive to the glass

  • Matter, atoms, and Elements

    • Matter - anything that occupies space and has mass

    • Structure of an atom

      • Nucleus - in the center with protons and neutrons

      • Electrons surround it 

  • Carbon and Hydrocarbons

    • Macromolecules - called organic molecules which means they contain carbon albums

    • Carbon bonds are strong (covalent bonds) - so carbon is usually the backbone of macromolecules 

    • Hydrocarbons - molecules consisted out of hydrogen and carbon only

      • They store a lot of energy

  • Chemical bonds

    • Ionic bonds - when atoms lose or gain electrons - form ions/ionic bonds

      • Cations - positive ions bc LOSE elections 

      • Anions - negative ions bc GAIN electrons

    • Covalent bonds - when atoms share electrons 

      • Key way that DNA/proteins are structured

      • More electrons shared, stronger the bond will be

    • Hydrogen bonds - polar covalent bond containing hydrogen

      • Hydrogen is slightly positive so it will be attracted to slightly negative 

  • Macromolecules 

    • Polymers - long chains made up of monomers 

      • Can be made of different monomers 

        • Ex. proteins made of 20 diff amino acids

        • Ex. dna made of 4 nucleotides

    • Carbohydrates, nucleic acids, and proteins are macromolecules

    • Phosphorus makes nucleic acids and lipids !!!

    • Dehydration synthesis - how large molecules assemble 

      • One monomer hydrogen bonds to another 

      • Covalent bond

      • Require energy to do reaction 

    • Hydrolysis - when polymers are broken down into monomers

      • When split one monomer gains a H group and one gains a OH- groups 

      • Releases energy

  • Carbohydrates

    • Carbohydrates - molecules made out of carbon, hydrogen, and oxygen

    • Monosaccharides - simple sugars - glucose

    • Sugars are ring shaped

    • Disaccharides - when two monosaccharides are join together because of dehydration synthesis

      • Form hydrogen bond and release water

    • Polysaccharides - long chain of monosaccharides

      • Starch - mixture of two polysaccharides (made of glucose)

    • Cellulose - what cell walls are made of 

  • Proteins

    • Primary structure

      • Linear sequence of amino acids

    • Secondary structure 

      • Forms a coil - alpha helix

      • Forms a zig zag - beta pleated sheets

      • Shapes based on R- groups 

    • Tertiary structure

      • Can have alpha and beta chains within

      • Bonds between R groups by Hydrogen, Ionic bonds, Disulfide bridges, Hydrophobic interactions

    • Quaternary structure

      • Same bonds as tertiary structure but bonds between entire peptide chains rather than R groups

Unit 2 - Cell Structure and Function

  • All cells have genomes and ribosomes

  • Ribosomes

    • Synthesize proteins according to mRNA sequence

      • mRNA comes from genome

    • 2 subunits

    • Made of rRna

  • Endoplasmic reticulum

    • Network of membrane tubules in cytoplasm

    • Rough ER

      • Has ribosomes attached to it 

        • Therefore carries out protein synthesis

      • Packages newly synthesized proteins for export from the cell

    • Smooth ER

      • Does NOT have ribosomes

      • Lipid synthesis

  • Golgi complex

    • Membrane bound sacs

    • Folding and chemical modification  of newly synthesized proteins

    • Packaging proteins

  • Mitochondria

    • Outer membrane - smooth

    • Inner membrane - folded

      • Capture energy from macromolecules (carbs, glucose)

      • Krebs cycle in the matrix

      • ETC and atp synthase in inner membrane

  • Lysosomes

    • Contain hydrolytic enzymes

      • Used to digest macromolecules or damaged cells

      • Recycle materials

      • Forced cell death

  • Vacuoles

    • Storage of water - for turgor pressure

      • Turgor pressure - caused by water pushing up against plasma membrane and cell wall

    • Release of waste products

  • Chloroplasts

    • Double outer membrane

    • Capturing energy and producing glucose 

    • Thylakoid - membrane compartments organized in stacks called grana

      • Chlorophyll pigments are in photosystems which are in the thylakoid membrane

      • Light reactions

    • Stroma - fluid between inner chloroplast membrane and outside thylakoids

      • Calvin cycle

  • Plasma membrane

    • Provides a boundary between the interior of the cell and outside environment

    • Control the transport of materials in and out of the cell

    • Phospholipids

      • Hydrophilic phosphate head 

      • Hydrophobic fatty acid tail (nonpolar)

      • Form a bilayer (tails interact with each other and heads interact with aqueous outside and inside)

    • Embedded proteins

      • Peripheral proteins - loosely bound to surface of membrane

        • Hydrophilic - interact with the polar heads

      • Integral proteins - span the membrane

        • Hydrophilic - charged side groups 

        • Hydrophobic - nonpolar middle groups

      • Membrane protein functions

        • Transport

        • Cell to cell recognition

        • Enzymatic activity

        • Signal transduction 

        • Intercellular joining

        • Attachment for extracellular matrix or cytoskeleton

  • Fluid mosaic - mosaic of proteins in fluid bilayer

  • Held by hydrophobic interactions (weaker than covalent bonds)

  • Proteins and lipids shift/flow across membrane

  • Cholesterol - steroid randomly wedged between phospholipids

    • Regulate bilayer fluidity under certain environmental conditions

  • Carbohydrates and lipids on membrane that act as markers

    • Glycoproteins

      • One or more carbohydrates attached to membrane

    • Glycolipids

      • Lipid with one or more carbohydrate attached

  • Membrane permeability 

    • Small nonpolar molecules pass freely 

      • N2, O2, CO2

    • Hydrophilic molecules (large nonpolar molecules and ions) can not pass freely through

    • Transport proteins

      • Channel proteins - tunnel spanning the membrane that allows target molecules to go through 

      • Carrier proteins - Spans the membrane and changes shape to move target molecules from one side to another

    • Cell wall 

      • structural boundary

        • Protects and maintains shape of cell

        • Prevents cellular rupture when turgor pressure is high

      • Permeable barrier

        • Plasmodesmata - small holes between plant cells that allow transfer of nutrients and ions

      • Made of cellulose in plant cells (polysaccharide)

    • Facilitated diffusion

      • Movement of molecules from high concentration to low concentration through transport proteins

      • Large and small polar molecules

      • Large quantities of water through aquaporins

      • Charged ions require channel proteins

    • Active transport

      • Moves molecules against their concentration gradient from low to high

        • Require pumps and use ATP

  • Water Potential

    • Water potential measures the tendency of water to move by osmosis

    • Calculated from pressure potential and solute potential 

    • Moves from area of high water potential to low water potential 

      • Values of water potential can be positive or negative or zero

        • The more negative the water potential is, the more likely water will move into that area

    • Water potential of pure water has a value of zero

      • There's no solutes

      • No pressure bc in open container

    • Osmoregulation - allows organisms to control internal solute composition and water potential

      • Increase solute in water = increase solute potential and decrease water potential

      • Increase water portnation = increase pressure potential 

    • Solute potential 

        • Ionization constant (ex. Sucrose = 1)

        • C = molar concentration (mmoles)

        • R = pressure constant

        • T = temperature in celsius + 273 = Kelvin

      • More solutes = negative solute potential

    • Diffusion - movement of molecules from high to low concentration 

    • Facilitated diffusion = movement of molecules from high to low concentration through channel proteins

    • Movement of large molecules in and out of cell

      • Endocytosis - forming vesicles derived from plasma membrane

        • Phagocytosis

        • Pinocytosis

        • Receptor mediated endocytosis

      • Exocytosis - internal vesicles fuse with membrane and put stuff out of the cell

  • Compartmentalization

    • All cells have a plasma membrane - maintain  different internal and external excitement

    • eukaryotic cells have additional membrane bound organelles that compartmentalize the cell

      • Allow for metabolic process and enzymatic processes to be at the same time = increased efficiency 

    • Example - lysosomes need to function in an acidic environment

      • Can maintain acidic inside while having more neutral outside environment

Unit 3 - Cellular Energetics 

  • Enzyme Structure

    • Enzymes - catalysts that speed up chemical reactions

      • Proteins - tertiary shape

      • Have an active site that is very particular 

      • Substrate that interacts with enzymes specific active site 

      • The active site can be altered to fit the substrate

      • Reusable - not chemically changed by reaction

      • Facilitate synthesis or digestion reaction

    • Enzyme names indicate the substrate or chemical reaction involved

      • End in -ase

      • Ex. sucrase is enzyme that digests sucrose

  • Enzyme Catalysis

    • All biochemical reactions require starting energy - ACTIVATION ENERGY

    • Some reactions result in net release of energy and some with a net absorption

    • Reactions w/net release require LESS activation energy

    • Enzymes lower activation energy required - accelerating rate of reaction

  • A controlled experiment is an scientific investigation - w/2 types of tests

    • Control group

      • Generates data under conditions with no treatment

      • Generates data under normal/unchanged conscious

      • Baseline data

    • Experimental groups

      • Data under abnormal conditions

      • Under treated/manufactured conditions

      • Compared with control test results to see impacts of treatment

  • Control groups

    • Negative control

      • Not exposed to experimental treatment or ANY treatment with an effect

    • Positive control

      • Exposed to treatment with a known effect 

      • Not exposed to experimental treatment

  • Enzymes have specific conditions for maximum function

    • Changing temp, ion concentration will interfere with the bonds (hydrogen and ionic) and change the shape of the active site

    • Enzyme can be denatured (function doesn't happen anymore)

  • Temperature effect on enzyme 

    • Optimum temperature - more kinetic energy increases molecular motion and increases the chance that enzyme bond with substrate

    • At too high temperature it will denature

  • Reversible denaturation - restoration of optimal conditions resorts the enzymes optimal shape

  • Irreversible denaturation - enzymes shape permanently changed and catalytic ability is destroyed

  • Substrate concentration effect on enzyme activity

    • Low concentration - probability of enzyme bumping into substrate is low and product produced at low rate

    • As substrate concentration inc, collision and rxn rate increase

    • Saturation point - when all the active sites of the enzymes are taken up

  • Competitive v noncompetitive inhibition

    • Competitive: foreign molecule that's not the enzymes substrate blocks the active site and inhibits the rate of reaction

    • Noncompetitive: foreign molecule binds away from the active side called allosteric site which has a ripple effect through the protein which causes a change in shep of the active site 

  • Metabolic pathway

    • Linked series of enzyme catalyzed chemical reactions within a cell

    • Example: 

      • 1, 2, 3, are enzymes

      • a is reactant

      • b and c are intermediate

      • d is the product

    • Examples are glycolysis, krebs cycle, etc

    • Can be linear (glycolysis) or circular (calvin/krebs)

  • Autotrophic - organisms that produce their own food

    • Photoautotrophs (plants, cyanobacteria) - use light energy to create organic components to survive through photosynthesis

    • Chemoautotrophs (bacteria) use the energy from chemosynthesis which oxidizes inorganic substances (iron, sulfur)

  • Heterotroph 0 capture energy present in organic compounds produced by other organisms

    • Consumers, decomposers 

    • Metabolizing the organic compounds in those that they eat or absorb

  • Exergonic - release energy and increase entropy

    • Example - burning stuff releases chaotic chemicals

    • Cell respiration and hydrolysis

  • Endergonic - require energy and decrease entropy

    • Photosynthesis or any dehydration synthesis

  • Structure + function of ATP

    • Structure - 5 carbon sugar ribose, nitrogenous base (adenine), 3 phosphate groups

    • Function - power work within cells (every cell makes its own atp)

    • Storing energy - cells take energy from food during cellular respiration and combine with ADP and phosphate groups to make ATP

    • Release energy for work - cells remove a phosphate group creating ADP + P

  • Energy coupling - linking an exergonic reaction to an endergonic one 

    • Drives the endergonic reaction forward

    • Ex: cell respiration (exergonic rxn) drives formation of ATP from ADP and P (endergonic)

    • Ex: exergonic ATP to ADP and P makes stuff like active transport possible

  • Photosynthesis

    • Using light energy - photoautotrophs use CO2 and H20 to create carbohydrates - O2 released

    • Source of biomass in every food chain

    • Endergonic rxn - takes two low energy inputs and converts them into high energy products

    • Reduces entropy (12 molecules on reactant side converted into 7 on the product side)

      • Co2 gas created into a solid object

  • Light Reactions - converts light energy into chemical energy (ATP and NADPH)

  • The Calvin Cycle - converts chemical energy in NADPH and ATP into carbohydrates

    • Fixes carbon dioxide - low energy gas into high energy sugars

  • Chlorophyll - pigment that absorbs light energy 

    • Absorption spectrum - shows the amount of light absorbed at different wavelengths

      • Chlorophyll has two forms, one that absorbs blue and red, one that DOES NOT ABSORB GREEN (plants r green bc green light bounces off)

    • Action spectrum - shows how different light wavelengths drive photosynthesis (blue and red does most while green doesn't)

  • Chloroplast - found in plant cells

    • Has outer and inner membrane 

      • Two membranes example of evolution of the membranes from a prokaryotic cell

    • Has DNA and ribosomes 

    • Has thylakoids - membrane bound sac 

      • Contain chlorophyll for light reaction 

      • Organized in grana stacks 

    • Stroma - cytoplasm of the chloroplast

      • DNA, ribosomes, 

      • Where calvin cycle

  • Light Reactions - occurs in thylakoids

    • Outputs - o2 is waste product

    • Inputs - light and water

      • NADP+ and ADP + P (from the calvin cycle)

  • Photosystems - proteins imbedded with chlorophyll

    • Convert light energy into a flow of electrons

    • Splits water molecules (photosystem 2)

    • Photosystem 2 comes before photosystem 1

    • A and d - light energy

    • Electron pathway - i, b, e, f

    • Proton pumps - c 

      • Protons from stroma to thylakoid space 

  • Photoexcitation of chlorophyll in PSII begins a flow of electrons along an ETC in the thylakoid membrane 

    • ETC powers the proton pumps from the stroma to thylakoid space

    • Result - chemiosmotic gradient powers ATP synthase as protons diffuse from the thylakoid space back to the stroma via ATP synthase

    • PS II - water splitting complex 

      • Splits water apart (CREATES OXYGEN) and also creates protons for the gradient

  • Calvin Cycle

    • Photoexcitation of chlorophylls in photosystem 1 

      • Creates flow of electrons of ETC of PS I

      • Electrons flow to NADP+ Reductase which NADP+->NADPH

        • Bc during calvin cycle NADPH to reduce CO2 to carbohydrates 

  • The Calvin Cycle

    • Carbon fixation phase

      • CO2 Is combined with RuBP

      • Reaction is catalyzed by RuBisCo

      • Six carbon product immediately dissociates into two 3 carbon molecule 

    • Energy investment and harvest phase

      • Starts with 2 three carbon molecules 

      • That 3 carbon product is reduced and phosphorylated 

        • ATP contributes a P to the molecule 

        • NADPH donates an electron to the molecule

      • End with G3P

    • Regeneration of RuBP

      • Remaining 5 G3P are rearranged into three 5-carbon molecules

      • Phosphorylation occurs 

      • RuBP is a substrate that starts the whole calvin cycle 

    • Pay attention to amount of carbon atoms in each phase

      • Starts with 3 RuBP (5 carbons each) combined with 3 carbon dioxide which is 18 Carbons

      • At the end of carbon fixation - 6 3 carbon molecules (18 carbon)

      • Duringenergy investment - 6 molecules of G3P

        • One G3P gets pulled out and is available to make carbohydrates 

      • During regeneration of RuBP - arranged into 3 five carbon RuBP

  • Cellular respiration 

    • Exergonic reaction - release energy and creates disorder

      • Takes organized glucose and converts to gasses

    • Glycolysis occurs in the cytoplasm and the link reaction brings the energy into the mitochondria

    • Krebs cycle where the matrix occur

    • oxidative phosphorylation - in the intermembrane space

  • Glycolysis

    • Energy in glucose generates NADH some ATP 

      • End product ends with 3-carbon pyruvic acid

    • Cytoplasm - does not require oxygen - aerobic - 3 phases

    • 1. Investment - enzymes phosphorylate glucose 

      • Take phosphate from ATP

    • 2. Cleavage - intermediate fructose is split into 2 G3P

    • 3. Harvest - G3P is oxidized (lose energy) and NAD+ is reduced to NADH

      • Enzymes phosphorylate two ATPs (from G3P)

    • Net yield of glycolysis is 2 

      • It's 4 but two get used at the beginning. 

      • Get 2 pyruvate from

  • Link Reaction 

    • Brings pyruvate into mitochondria matrix and converts it to Acetyl CoA (2 carbons)

      • generates NADH 

      • Releases one CO2 (one carbon)

        • 1./3 of the co2 released

    • Other enzymes oxidize the resulting two-carbon molecule (acetyl) reduces NAD+ to NADH

    • Enzymes take acetyl and attach it to coenzyme A = acetyl COA -> start of krebs cycle

  • Krebs cycle 

    • oxidizes acetyl CoA - to produce 3NADH, 1 ATP, 1 FADH2 and releases 2CO2

    • In mitochondrial matrix

    • Starts w/enzymes taking Acetyl CoA to become citric acid 

    • Enzymes start to oxidize citric acid (loss of electrons)

      • Electrons go to NAD+ -> NADH

    • Other enzymes power substrate level phosphorylation of ADP and P into ATP

    • For each acetyl CoA that enters cycle - one ATP, one FADH, and 3 NADH are generated

      • CO2 us waste product

  • Electron transport chain - ETC and oxidative phosphorylation

  • Oxidises NADH and FADH2 to create electron flow to power phosphorylation of ADP to ATP (ONE)

  • Those electrons go through ETC in mitochondrial inner membrane

  • Some ETC proteins are proton pumps that pump protons into matrix

    • Active transport that gets energy from the electrons 

    • Creates electrochemical gradient

  • Oxygen acts as the final electron acceptor

    • Bc so electronegative that it pulls electrons down

  • Protons in intermembrane space go through ATP synthase

    • As protons diffuse through - energy is used to create ATP

  • Anaerobic v Aerobic respiration

    • Aerobic - oxygen is required

      • Glycolysis + Link + krebs + ETC = 32 ATP for every glucose molecule

      • Most ATP generated in mitochondria

    • Anaerobic respiration (when o2 isn't present, is insufficient, or when organism does not have enzymes to do aerobic)

      • Glycolysis is the key part followed by fermentation = 2 ATP

      • Occurs in cytoplasm

  • Fermentation

    • Glycolysis followed by reactions that generate NADh

    • Why: when aerobic isn't working but you still need to work, you can still get energy 

  • alcoholic fermentation v lactic acid fermentation 

    • Ethanol fermentation - in yeast

      • Enzymes remove C02 from pyruvic acid

      • Produces ethanol and NADh oxidized to NAD+

        • Allows glycolysis to continue

    • lactic acid fermentation 

      • Occurs in muscle during anaerobic condition

      • Pyruvate reduced to lactic acid and NADH is oxidized to NAD+

UNIT 4 - Cell Communication and Cell Cycle

  • Communication through direct contact

    • Cells of multicellular organisms maintain physical contact with one another 

    • Unicellular organisms usually live in colonies that are in physical contact with other organisms in that colony

    • Cells can send chemical signals directly into adjacent cells

    • Cell membrane and cell wall mods allow for communication to occur between adjacent cells 

  • Communication 

    • Cells use chemical signals for short and long distances 

    • The cell receiving the signal is the target cell

    • Short distance

      • Cells send out local signals

      • Target cell is within short distance

      • Used to communicate with cells of the same type

    • Long distance

      • Target cell in diff area as the cell emitting the singla

        • Ex signal from head to stomach

      • Signal travels larger distance to reach target cells

      • Uses signal cells of different types

  • Example - neurotransmitters communicate using local regulators

    • Exocytotic vesicles release signals

    • Signal travels short distance between gap

    • Signal attach to target cell and cause response

  • Signal transduction pathways (STP)

    • STP link signal reception with cellular responses

    • 3 steps of cell communication are reception, transduction, and response

      • Reception: detection of signal molecule coming from outside of the cell

      • Transduction: convert signal to a form that can bring about a cellular response

      • Response: specific cell response to the signal molecule

    • STP include protein modification and phosphorylation cascades

      • Regulate protein synthesis by turning on and off genes in nucleus

      • Regulate activity of proteins in cytoplasm

      • Cascades of molecular ineracts relay signals from receptors to molecules

      • Phosphorylation cascades - enhance and amplify signal

  • Signaling

    • Begins with recognition of chemical messenger (ligand) by a receptor

      • Ligand binding domain of a receptor recognizes specific chemical messenger which is a specific one to one relation

      • G-protein coupled receptors 

    • Hormone binds to receptor which converts GDP to GTP which goes through adenylate cycle which converts ATP to cAMP which activates protein kinase

    • Cascades - relay signals from receptors to target cells - amplifying incoming signals (cell growth, gene expression)

      • After ligand binds, domain of receptor changes shape initiation transduction of signal

      • Second messengers (cAMP) relay the message

    • Takeaways:

      • Signal transduction pathway is the binding of signaling molecules to receptors on cell surface that trigger events inside the cell that invoke a response

      • Cells use signal transduction with a cellular response (growth, gene expression)

      • Signal transduction pathway begins with a ligand bonding to external receptor

      • Role of protein modification in STP is to change shape due to ligand bonding

      • Phosphorylation cascade is a signaling pathway where one enzymes phosphorylates another causing an amplification of the pathway

  • signal transduction pathways influence how a cell responds to its environment

    • Environment is not static - organisms always need to regulate pathways to respond to changes

    • Ability to respond to stimuli is necessary for survival

Example

  • Epinephrine is ligand that bonds to signaling molecule

  • It activates the G protein 

    • GTP active

    • G protein activates adenylyl cyclase which converts ATP to cAMP 

  • cAMP activates protein kinase A which leads to the response (glycogen breakdown)



  • Signal transduction results in changes in gene expression and cell function

    • Pathways can target gene expression/amount of protein produced

    • Changes in protein taupe can be a phenotype change

    • Apoptosis can be response of signal transduction

  • Changes in signal transduction pathways

    • Changes in signal transduction pathways alter cellular response

    • Mutations in any domain of receptor protein effects the rest of the transductions pathway

    • Chemicals that interfere with the pathway may inhibit or activate the pathway

  • Feedback mechanisms

    • Helps organisms to maintain internal homeostasis and respond to environmental changes

    • Homeostasis - maintenance of stable internal environment

    • Processes that maintain homeostasis by increasing or decreasing response to cellular event

  • Negative feedback - maintain homeostasis for particular cell condition

    • If process disrupted, negative feedback helps it return to homeostasis 

  • Positive feedback - amplify responses in organisms

    • A variable initiates a response to move further away from homeostasis

    • Amilpfy that stimulus which creates an entire system change 

    • Example: fruit ripening

      • When one apple ripens, it releases gas that is detected by other apples

      • Other apples start to ripen and m0re gas is released 

      • RIPENING APPLES IS MOVING FURTHER FROM HOMEOSTASIS

  • The cell cycle - series of events for growth and reproduction of cells

    • Interphase - growth and preparation for cell division

      • G1 - cell growth

      • S phase - copies of DNA are made 

      • G2 - cytoplasmic components are doubled - ready for division

    • mitosis - division of nucleus

      • Can be asexual reproduction for some organisms

      • Process that splits into two daughter cells with identical genomes identical to parent cell 

    • Cytokinesis - split cell 

      • Equal split of cytoplasm to daughter cells

      • Come cells enter G0 where cell division no longer occurs 

        • Can re enter cycle with appropriate signal

  • Phases of mitosis

    • Prophase 

      • Nuclear envelope begins to disappear

      • DNA coils into visible chromosomes (chromatid)

      • Fibers begin to move chromosomes to middle of cell

    • Metaphase

      • Fibers aligned double chromosomes across cell

    • Anaphase 

      • Fibers separate double chromosomes into single chromosomes

      • Separated at centromere 

      • Chromatids migrate to opposite sides of the cell

    • Telophase

      • Nuclear envelope reappears and 2 separate nuclei

      • Each nucleus contains complete genome

      • Chromosomes begin to uncoil

    • Cytokinesis

      • Separate cell into two daughter cells (cell membrane forms)

  • Regulation of the cell cycle

    • Regulated through checkpoints

    • G1 checkpoint

      • At the end of the G1 phase 

      • Cell size, nutrient, growth factor, DNA damage check

    • G2 checkpoint

      • DNA replication check

      • DNA damage check

    • M-Spindle checkpoint 

      • After metaphase

      • Making sure fibers attached to chromosomes accurately so it splits correctly

  • Cyclins 

    • Group of related proteins associated with specific phases of the cell cycle

    • Concentrations can fluctuate depending on cell activity

      • Promote cell cycle progression

      • Degraded to inhibit cell cycle progressing

    • Used to activate cyclin dependent kinases 

  • Cyclin dependent kinases (enzymes)

    • Requires cyclin binding for activation 

    • Phothotolate substrate and promote cell cycle 

  • Disruptions in cell cycle that lead to cancer/apoptosis 

    • A cell with a genetic alteration/mutation may singal to go through apoptosis

    • Genetic alteration where cells skip past checkpoints and just continue to divide -> cancer

UNIT 5 - Heredity

  • Meiosis 

    • Formation of haploid gamete 

    • Diploid

      • 2 full sets of pairs of chromosomes

      • Chromosomes differ in size shape etc

      • Cell contains one set from each parent

      • Body cells are diploid

    • Haploid

      • Contain one set of chromosomes

      • Gametes - sex cells

    • 2 haploid cells come together to produce a diploid cell

    • Diploid parent creates 4 haploid/daughter sex cells

  • Two phases of meiosis - meiosis 1 and 2

    • Meiosis I 

      • Prophase I

        • Nuclear envelope disappear

        • Fibers form

        • Dna coils and duplicated chromosomes are sister chromatids

        • Double chromosomes pair up 

        • While pair up, chromatids exchange information by crossing over

      • Metaphase I

        • Double chromosomes remain in pairs

        • Fibers allison them across center of cell

      • Anaphase 1 

        • Fibers separate chromosome pairs

        • Chromosomes move to opposite sides

      • Telophase I

        • Nuclear envelope reappears and 2 separate nuclei

        • Each nucleus has one double chromosome

          • Nucleus only contains half of the total info that parent had

            • But still double chromosomes

      • After cytokinesis - two HAPLOID daughter cells

    • Meiosis II

      • THIS IS EXACTLY LIKE MITOSIS

  • Mitosis v meiosis 

    • Both 

      • Nucleus disappear

      • Dna -> chromatids

      • Align in center + fibers separate

      • Nuclear envelope reappear

      • Cytokinesis 

    • Different 

      • Mitosis - goal is to make 2 daughter cells while meiosis it creates 4 haploid daughter cells diff from parent cell

  • Meiosis and genetic diversity 

    • Meiosis results in 4 genetically different gametes

    • CROSSING OVER

      • Occurs in prophase I 

      • Nonsister chromatids of double homologous chromosomes exchange segments/genes

      • Recombinant chromatids -> genetic diversity

    • Random assortment of chromosomes

      • Order of homologous pairs during meta I effects which chromosomes end up in each gamete

    • Fertilization of gametes increase variation

      • During fertilization, info from each parent contributes 

        • One gamete from each parent to form diploid offspring

      • Fertilization is random and any gamete can become the genome of the offspring

  • Continuity of life

    • Heritable information provides for continuity of life

      • Nucleic acids (DNA and RNA) carries genetic information in their order

      • That genetic info is transferred to other cells during division

      • Transmission of genetic info from one gen to another = continuity of life 

    • Shared, conserved processes and features → common ancestry

      • Genetic code is shared by ALL living systems

      • All organisms use nucleic acids to store genetic information

      • All organisms have ribosomes to synthesize proteins based on nucleic acid sequences

      • Core metabolic pathways are conserved across all domains of life

        • Ex. cell respiration used by all organisms 

          • Glycolysis is in anaerobic and aerobic respiration

  • Mendailian genetics 

    • Mendel's laws - describe inheritance of genes on diff chromosomes

      • A gene is unit of heredity coding for trait

        • Can be transferred through generations

      • Trait - characteristic of gene

      • Allele - specific variation of genes

        • Alleles from each parent

        • Organisms can inherit different alleles for the same genes

        • Dominant allele - its the trait that is shown

        • Recessive allele - only shown when dominant allele isn't present

      • Genotype - combination of of inherited alleles

        • Homozygous - 2 of the same alleles

        • Heterozygous - two different alleles

      • Phenotype - physical result of genotype

    • Law of segregation

      • Chromosomes carry alleles

      • Homologous chromosomes carry alleles for the same trait

        • The allele could be different

      • When chromosomes separate during meiosis - alleles are also separated

      • Example: mendel's experiments = specific crosses

        • P generation - homozygous dominant crossed w/homozygous recessive

          • The F1 generation all ended up with purple flowers 

        • F1 generation - he crossed two heterozygous plants 

          • F2 generation ended up having 3 purple and one white 

        • Reappearance of white flower - evidence of law of segregation 

          • Recessive alleles from each parent packaged into diff gametes 

    • Law of independent assortment

      • Two or more genes assort independently of each other 

      • One trait does not automatically have to be inherited with another trait

      • Alleles for separate traits can be packaged into every possible combination of gametes

  • Crosses 

    • Monohybrid cross - examination of how one trait is inherited

      • Two possible phenotypes (R and r)

      • Three possible genotypes (RR, Rr, and rr)

    • Dihybrid cross - examination of how two traits are inherited 

    • Punnett squares - outcomes of cross 

  • Pedigrees - visual of representation tracing history of trait through generations

    • Predicts patterns of inheritance

    • Helps to identify types of inheritance

    • Circles - females 

    • Squares - males 

    • Shaded shapes - individuals affected 

  • Chi squared hypothesis testing

    • Hypothesis testing - reject/fail to reject hypothesis

      • Determines if differences in numerical data are due to the independent variable or by chance

    • 2 types of hypothesis 

      • Null Hypothesis - states there is no difference between the two groups of data in investigation 

      • Alternative hypothesis - observed results are due to non random results

    • Chi squared goodness of fit test 

      • Used to determine if there's significant relationship between two groups of data

      • Compared observed outcomes to expected outcomes

    • Steps to chi squared test

      • Establish question

      • Determine null hypothesis

      • Determine alternative hypothesis

      • Count observed valued

      • Determine expected values 

      • Calculate chi squared 

      • Calculate degrees of freedom 

      • Use p value

      • Compare chi squared value to critical value and draw conclusion

  • Nonmendalian genetics

    • Patterns of inheritance do not follow rations predicted 

    • Genes that are adjacent and close together on the same chromosome that are inherited together are linked genes

    • Traits determined by genes on sex chromosomes are sex linked traits

    • Linked genes

      • Typically inherited together

      • Less likely to be separated during meiosis

    • Probability that genetically linked genes will segregate as a unit can be used to calculate the map distance between them

      • Map distance 

        • Tells you how close a pair of linked genes are

        • Determined by how frequently a pair of genes participates in a crossover event

        • Linked genes have recombination frequency of less than 50%

        • Ex. if a pair of linked genes have recombination frequency of 30% (they are separated by crossing over 30$% of the time) then that means they are 30 map units apart

    • Sex linked traits - located on sex chromosomes

      • Determine biological sex in animals

      • Non homologous 

      • X and Y letter designations

      • They deviate from mendel mode of inheritance

  • Some traits result from non-nuclear inheritance

    • Chloroplast and mitochondria contain non-nuclear genomes

    • Chloroplast and mitochondria are randomly assorted into gametes and daughter cells during cell division bc they are just in the cytoplasm

    • Mitochondria are transmitted to the egg, not the sperm in animals !!

      • Mitochondrial traits are maternally inherited

    • Traits determined by chloroplast and mitochondrial DNA do not follow medallion rules

    • Can be determined through pedigree

      • If female is affected by trait, all her children will be too

  • Environmental effects on phenotype

    • The same genotype can result in multiple phenotypes

      • Environmental factors influence gene expression

      • Phenotypic plasticity is the ability of one genotype to produce more than one phenotype 

      • Example - flower color based on soil pH

        • In hydrangeas - color of flower is based on pH of soil

  • Chromosomal inheritance

    • Law of segregation explains separation of alleles during gamete formation

      • Each gamete has one allele for each gene

      • Provides opportunity for more varied alleles

    • independent assortment - suggests that genes for two or more traits sorted into gametes independently

      • Assortment of genes independently provides more possible gene combinations

    • Random fertilization - concept that any genetically unique sperm and egg can combine

  • Mutated alleles can be inherited (genetic disorders)

    • Chromosomes can carry mutated alleles

    • Law of segregation/independent assortment explain how mutations alleles can be distributed into gametes

    • Genetic disorder if mutation negatively effects

    • Nondisjunction - failure of chromosomes to fully separate in the formation of gametes 

      • Too many or too few chromosomes

      • Example - triple x syndrome (3 x chromosomes)

    • Triploidy is having three copies of a particular chromosome which results in sterility in some animals

    •  polyploidy is having multiple sets of homologous chromosome which is seen mostly in plants 

UNIT 6 - Gene Expression and Regulation

  • DNA is the primary genetic material

    • Genetic information is stored in sequence of bases

    • Genetic info is transmitted from one generation to the next

    • DNA is packaged into chromosomes before its passed to daughter cells

    • Viruses use RNA

  • DNA and RNA are structurally similar

    • Both polymers containing nucleotides

    • Chain like molecules

    • Base pairing rules 

      • DNA - adenine and thiamine 

        • Guanine and cytosine

      • RNA - adenine and uracil

        • Guanine and cytosine

  • Specific base pairing is conserved through evolution

    • DNA and RNA both follow base pairing rules 

      • Pyrimidines pair only with purines 

      • Pyrimidines - single ring structure 

        • Uracil, cytosine, thiamine

      • Purines - double ring structure

        • Adenine and guanine

    • Prokaryotic and eukaryotic genomes differ

      • Prokaryotic organisms have circular chromosomes while eukaryotes have multiple linear chromosomes

      • Prokaryotic genomes are smaller than eukaryotic genomes

      • Prokaryotes and eukaryotes can contain plasmids (small extrachromosomal double-stranded circular DNA molecules)

        • Prokaryotic plasmids are in cytosol in eukaryotes they are found in the nucleus 

  • Replication

    • Purpose of replication - ensure continuity of heredity information

    • Genetic information copied before cell division

      • Transmission of genetic information from one generation to another

    • Semiconservative replication - in a DNA molecule, one is the original stand and one is a newly synthesized element

      • Each original strand is the template for the complementary strand

    • Directionality influences replication process

      • Each DNA has phosphate group on one end and hydroxyl group on the other end

        • 5’ and 3’ end

        •  two strands of DNA run antiparallel to each other 

        • Nucleotides can only be added in the 5 to 3 Direction

        •  Strand always synthesized continuously - leading strand

        • Strand always synthesis discontinuous in fragments - lagging strand

  • Enzymes involved with DNA replication

    •  helicase unwinds the DNA strands 

    • Topoisomerase relaxes the super coil at the replication fork

      •  replication fork is where the two strands are separated 

    • DNA polymerase synthesizes new strands

      •  it requires RNA primers to initiate synthesis

      •  it attaches to the 3 end of the template

      •  builds strands in a 5 to 3 Direction

    • Ligase joins DNA fragments on the lagging strand

      •  it connects them together

  • Genetic information flows from DNA to RNA to protein 

    • Ribosomes contain rRNA that assemble proteins 

  • Transcription-  the process in which an enzyme directs the formation of an mRNA molecule 

    • DNA strands separate during transcription

      •  one strand is the template strand also known as The non-coding Strand

      •  the other strand is the non-template Strand also known as the coding strand

      •  The Strand serving as the template depends on the gene being transcribed

        •  the gene targeted for transcription is on the coding strand 

    • The enzyme RNA polymerase synthesizes mRNA in the 5 to 3 Direction by reading the template in a three to five Direction 

  • Three types of RNA

    • mRNA - carries genetic info from DNA to the ribosomes

      • Information is used to direct protein synthesis at the ribosomal site 

      • A codon is a three base sequence on mRNA

    • TRNA -  recruited to the ribosomes to help create specific polypeptide sequins directed by MRNA

      •  various tRNA molecules each carrying a specific Amino

      •  the anticodon is a three-based sequence on TR

      •  correct base pairing of TRNA anticodons with mRNA codons release in the addition of an amino acid to a growing polypeptide

    • rRNA (ribosomal RNA) -   molecules are functional units of ribosomes responsible for protein assembly

      •  base pairing of anticodons and codons occur in the ribosome 

  • Transcription and RNA processing 

    • In eukaryotes-  a series of enzyme regulated modifications occur to the MRNA transcript 

    • Addition of a poly a tail

      •  100 to 200 adenine nucleotides

    •  addition of a GTP cap

      •  modified guanine nucleotide

      •  helps ribosomes attach to mRNA 

    • Introns - sequences of mRNA that do not code for amino acids

      • Removed during RNA processing

      • Not included in mature mRNA

    • Exons -  sequences of mRNA that do code for amino acids

      •  they are retained during RNA processing

      •  they are connected together in the mature mRNA 

    • Splicing introns and connecting retained exons = alternative splicing

      • Many different exons on a primary transcript

      • Different mRNA transcripts from one primary transcripts

        • Exons can be retained in different variations

        • Different mRNA transcripts lead to different proteins

  • Translation of mRNA generates polypeptide

    • Translation occurs on ribosomes

      • Prokaryote v eukaryote

        • Prokaryotes only have cytoplasm ribosomes

        •  eukaryotic cells can have cytoplasm or ribosomes bound to the rough endoplasmic reticulum 

    • Three main steps 

      • Initiation

      • Elongation

      • Termination

    • Retrovirus translation is a special case because of alternate flow of information

      •  retroviruses introduced viral RNA instead of DNA into host cells 

      • reverse transcriptase is an enzyme that copies the viral RNA into viral DNA (reverse process to transcription)

        •  reverse transcriptase is a viral enzyme

        •  once the enzyme converts viral RNA into viral DNA the DNA is integrated into the host gen

          •  once integrated the viral DNA will be transcribed and translated

          •  transcription and translation of viral DNA results in assembly of a new viral progeny 

  • Translation - final process in flow of info from DNA to RNA to protein

    • Converting RNA to protein

    • Initiation - first step

      • rRNA interacts with mRNA at first start codon

        • mRNA codon chart - helps Determine which codon codes for each amino acid

          •  some amino acids are coded for by more than one codon 

        • AUG is the start codon - methionine

        • Stop codons - UAA, UAG, UGA

    • Elongation - second step

      • Each newly arrived tRNA brings another amino acid to a growing peptide chain

        • rRNA adds the amino acid as tRNA brings it

    • Termination - last step 

      • Amino acids will continue to be added until a stop codon is reached

      •  the newly synthesized polypeptide is just released 

  • Regulation of gene expression

    • Differences in the expression of genes account for phenotypic differences between organisms 

    • Gene expression -  process where instructions and the DNA are transcribed and translated into a functional protein 

      • Different chemical reactions regulate gene expression

    • Regulatory sequences or stretches of DNA that can be used to promote or inhibit proteins synthesis

    • Regulatory proteins - assist promotion or inhibition of protein synthesis 

    • Epigenetic changes involve reversible modifications of DNA or histones

      •  histones are protein that DNA wraps around

        •  slight chemical modifications of DNA and histones cause tight or loose packing of DNA 

    • Observable cell differentiation results from the expression of genes for tissue specific protein 

      • Cells within multicellular organisms all have same DNA sequences

      • Tissues are groups of cells with the same function

        • Presence of specific proteins in tissues gives tissues specific functions 

      • Phenotype of a cell is determined by combination of genes expressed

      • Cell differential - Cells are in the same organism that had different phenotypes 

  • Induction of transcription factors during Development results in sequential gen

    •  transcription factors are proteins that promote or inhibit transcription of gene 

    • Presence of transcription factors helps determine how a cell will differentiate 

  • Both eukaryotes and prokaryotes have genes that are coordinately regulated

    • Operons are closely linked genes that produce a single mRNA molecule during transcription 

      • They are under control of the same regulatory sequence

      • Includes genes to be transcribed, regulatory sequence, and operator

        • Operator is a sequence that either it's or promotes transcription by binding with the regulatory protein 

    • In prokaryotes groups of genes called operons are transcribed in a single mRNA

      •  structural proteins with related functions are typically encoded together in the genome

        •  controlled by a single regulatory sequence

      •  regulatory genes can control the expression of all the genes at the same time 

    • The lac operon  is an example of an inducible operon

      •  it's inducible because it's usually turned off 

        • When the regulatory protein is bound to the operator RNA polymerase cannot bind to the regulatory sequence 

    • Inducers are molecules that can bind to the regulatory protein and cause it to change shape 

      • It causes the regulatory protein to release from the operator and frees up on a polymerase to transcribe the operon

    • Example - other transcription factors that regulate the lac operon

      • The amount of glucose in the cell helps regulate Gene Expression

        •  when glucose is low other transcription factors bind to the regulatory sequence which can promote transcription

        •  when glucose levels are high these transcription factors are not present 

  • Gene expression and cell specialization 

    • Princess in the expression of genes account for phenotypic differences between organisms

    •  Promoters -  DNA sequences that are Upstream of the transcription start site where RNA polymerase and transcription factors bind to initiate transcription 

    • The interaction between regulatory proteins, Regulatory genes, and transcription factors initiates transcription 

    • RNA polymerase is able to transcribe genes in the presence of transcription factors 

      • Negative regulatory molecules can inhibit gene expression by blocking transcription

        • RNA polymerase is blocked from binding to the promoter 

  • Mutations 

    •  Changes in genotype can result in changes in phenotype

      •  mutations are the changes in the genome 

        • Mutations can be positive negative or neutral based on the effect or lack of effect they have on the protein 

      • Substitutions - when one nucleotide is substituted for another 

        • Neutral if the change encodes the intended protein

        •  positive if the change benefits the cell or organism

        •  negative if the change harms the cell organism 

      • insertions/deletions

        • Shifts the rest of the codons/amino acids

    • The processing of genetic information is imperfect which leads to genetic variation

      •  disruptions in genes cause new phenotypes 

    • Random mutations caused by: 

      • Errors in DNA replication

      • Error in DNA repair

      • Radiation

      • Reactive chemicals

    • Mutations are the primary source of genetic variation

  • Horizontal acquisition of genetic information and prokaryotes increases variation 

    • Transformation-  refers to the uptake of naked DNA

      •  naked DNA is DNA not protected by proteins and it comes from an external environmental source 

    • Transfiction - transmission of foreign DNA into a cell when a viral genome integrates with the host genome (think of the bacteriophage (i think))

    • Conjugation - cell to cell transfer of DNA

      • Allows small segments of DNA to move from one cell to another

    • Transposition - movement of DNA segments within DNA molecules (cross sign over)

  • Biotechnology 

    • Genetic engineering tehnoques Can be use to analyze and manipulate DNA and RNA

      •  Electrophoresis

        • Separates molecules based on size and charge

        • DNA molecule are negativity charged 

        • Put DNA on negaitve side bc moves towards positive

        • It separated DNA fragents by size

          • Smaller will move further through gel

      •  polymerase chain reaction(PCR)

        • DNA fragments are amplified 

        • Allow scientists to create large samples of DNA when only small samples are available 

        • Steps 

          • DNA is denatured (seperating helix)

          • Primers are added

          • DNA is replicated 

          • Keeps doubling DNA

      •  DNA sequencing 

        • Determines order of nucleotides in DNA molecule

        • Nucleotides being labeled with dye to read DNA

UNIT 7 - Natural Selection

  1. Intro to evolution + natural selection:

    1. Natural Selection: any population of organisms, there's variation

      1. Process where environmental factors will select certain variations

      2. Some variations don't matter but some do a lot 

    2. Example: bacteria culture

      1. Every 1 in a billion are different 

        1. Bc of factors like mutations/random changes

      2. Antibiotic that kills the majority, but doesnt kill the 1 in a billion, so that continues to grow and the bacteria/virus will keep growing

  2. Biodiversity + Natural Selection

    1. Evolution: 2 pathways - Pattern + Process

      1. Pattern: shape of evolution

        1. Relationships of organisms overtime

        2. Phylogenetic tree

          1. Diff organisms/events that link organisms together

      2. Process: drivers of diversity

        1. Ex: all 4 legged animals have similarity but some differences about other aspects

      3. Natural Selection: fitting the circumstances of the environment + surviving

        1. Fitness: better able to produce offspring that had beneficial traits of the parent - Survival of the fittest

      4. Adaptations: traits that make better to survive/reproduce 

        1. Adapt to an environment that can change. 

Hardy Weinberg Equilibrium

  1. Allele Frequency

    1. Allele: gene variant

      1. Frequency of allele = (number of copies of allele X in population)/(total number of copies of gene in population)

    2. Example: B = brown eyes   b = blue eyes - 2 people starts with: one Bb and one bb

      1. Frequency of B (f(B)) = ¼ (25%)

      2. Frequency of b (f(b)) = ¾ (75%)

      3. Diff than phenotype % which would be 50% brown eyes and 50% blue eyes

    3. p is frequency of dominant allele and q is frequency of recessive allele

      1. p+q = 1

  2. Hardy Weinberg Equation

    1. Hardy weinberg principle assumptions - stable allele frequencies

      1. No natural selection that would change allele frequency (no more fit allele)

      2. No mutations 

      3. Large populations

    2. p + q = 1 (bc two alleles) (they both equal 100%)

      1. Square both sides : (p+q)2 = 12

        1. p2 + 2pq + q2 = 1

      2. p2 = p*p (frequency of dominant allele)2

        1. Probability of getting TWO dominant alleles

        2. Probability of someone being homozygous dominant

      3. q2 = q*q (frequency of recessive allele)2

        1. Probability of getting TWO recessive alleles

        2. Probability of being homozygous recessive

      4. 2pq = pq + qp

        1. Probability of getting heterozygous

  3. Applying the hardy weinberg equation

    1. Example: B=brown  b=blue

      1. 9% has blue eyes 

        1. Can we figure out p and q?

Evidence for Evolution

  1. Evidence for evolution

    1. Structural

      1. Homologous structures: things that have similar position/structure but maybe not the same function

        1. Ex: forearm: humans don't use it, dogs walk on it, birds use it to fly and whales use it to propel in water

        2. All the orgs had a common ancestor

      2. Fossil records: fossils from millions of years ago relate/change into current day organisms

    2. Microbiology/genetics

      1. translating/replicating of dna is very similar between all life

      2. Dna coding for proteins that have amino acids

      3. Humans are very similar to chimpanzees, then mice, then fruit flies then yeast, then a plant

        1. This helps determining how far common ancestors are

    3. Direct observation

      1. Bacteria: using antibiotics, there are some bacteria that are immune, those are the most fit, and the ones that reproduce

Phylogeny

  1. Taxonomy and the tree of life

    1. Taxonomy (carl linnaeus) - classification of organisms

      1. Group species into groups - genus (more specific), class, family, order, class, kingdom(more broad)

      2. Example: humans are homos, so were neanderthals (thats a genus)

        1. Homos and chimpanzees are in the same TRIBE as each other called the homoinus

        2. Homoinus are in the same FAMILY (called the hominidae) as the hominae which includes the gorilla

  2. Understanding and building a phylogenetic tree

    1. Just remember that worksheet

Speciation

  1. Species - animals that can interbreed and the offspring are fertile

    1. Example: lion x lion = lion

      1. Lion x tiger = liger 

        1. Liger not fertile so lion and tiger are different species

    2. Could be animals that act the exact same but look different

      1. Dogs are all the same species but can look different

        1. Ex. different dog breeds can mix

  2. Speciation

    1. Allopatric speciation - Formation of new species through geographic separation

      1. Ex. squirrels and the grand canyon

        1. They were the same species but as time went on and the canyon was created, they couldn't mate with each other so their genes changed

    2. Sympatric speciation - behavioral divergence

      1. Ex. american maggot fly has two options to mate on - hawthorne and and apple

        1. Some of them choose the hawthorne and some choose the apple and though the same geographical location, they diverge into two different species because of behavior

  3. Reproductive isolation

    1. Asexual reproduction: one cell reproduces and creates cells that are the exact same as the parent cell

      1. Low genetic diversity

    2. Sexual reproduction: 2 organisms reproduce and create completely different offspring

      1. High genetic diversity

    3. Species divided by reproductive isolation

      1. Prezygotic isolation - different forces that prevent two organisms from having offspring together BEFORE creation of zygote

        1. temporal/habitat isolation - two organisms mate at different times/places

        2. Behavioral isolation - attract by singing song etc

        3. Mechanical isolation - mating is not possible bc of size

          1. Elephant x mouse wouldn't work

        4. Gametic isolation - It's impossible for two gametes to produce a zygote bc of chromosome number

      2. Postzygotic isolation

        1. Zygor mortality - create zygote but has high mortality rate

        2. Hybrid inviability - zygote creates offspring but offspring has high mortality rate

        3. Hybrid sterility - zygote grows into adult but is infertile


UNIT 8 - Ecology

  • Organisms respond to changes in their environment through behavior and physiological mechanisms

    •  a stimulus is an external or internal signal that causes a response from an organism 

    • Organism exchange information with one another in response to internal and external signal 

      • Signaling behavior

        • Produces changes in other organisms 

          • Leads to differential reproudctuve success

    • Communication mechanisms

      • Visual, audible, tactile, electrical, chemical

      • Uses

        • Dominance, find food, establish territory, ensure reproductive success

    • Response and communication impact natural selection and  evolution

      • Natural selection favors behaviors that increase survival and reproructive success

    • Innate Behaviors are controlled and can occur without prior experience or training

    •  learn behaviors are developed as a result of experience

    •  Cooperative behaviors involve teamwork between organisms of the same species 

      • Increases Fitness of individual and survival of population

      • Example: warning traits

        • Discourage predation

        • Aposematism

        • Markings, behaviors, chemicals

          • Ex. coral snakes have red and yellow back banding that indicates they are venomous

  • Energy flow through ecosystems

    • Organisms use different strategies to regulate body temperature and metabolism

      •  endotherms use thermal energy generated by metabolism to maintain homeostatic body temperatures 

        • Ex. change in heart rate/fat storage

      • Ectotherms lack efficient internal mechanisms to regulate and maintain body temperatures

        •  rely on behaviors to regulate temperature example moving into or out of the Sun 

    • There is a relationship between metabolic rate per unit of body mass and the size of the organism 

      • Metabolic rate is the amount of energy expended by an animal over a specific amount of time 

        • Net gain in energy = energy storage/growth

        • Net loss of energy = loss of math or death

        • smaller the organism the higher the metabolic rate

    • Different organisms use various reproductive strategies in response to energy availability

      •  some species produce a lot of Offspring at one time

        •  less energy efficient

        •  more common in unstable environments where resources are not available

      •  some species produce very few Offspring at one time

        • More energy efficient

        •  more common in stable ecological environment 

    • Changes in energy availability can result in changes in population size ( disruption to ecosystem ) 

      •  a change in energy resources such as sunlight can affect the number and size of the trophic levels

      •  a change in the producer level can affect the number and size of other trophic levels

        •  A trophic level is a position an organism occupies in a food chain

    • Food chain - shows direction of nutrient and energy transfer from one organism to another

      • Each organism occupies a different trophic level and reflects how many energy transfers separated from the producer

        •  example: primary  producer to primary consumer to secondary consumer to tertiary consumer to Apex consumer to decomposer

    • Food webs consist of many interconnected food chains

    •  the transfer of energy between trophic levels is inefficient around 10% efficiency

      •  the Energy Efficiency limits the length of food chains and the size of population

      •  population size decreases as trophic level goes up 

    • The activities of autotrophs and heterotrophs enable the flow of energy within an ecosystem

      •  autotrophs are organisms that capture energy from physical or chemical sources in an environment

        •  photo autotrophs capture energy presence sunlight

        •  chemoautotrophs capture energy from small inorganic molecules in their environment without oxygen

      •  heterotrophs capture energy present in physical carbon compounds produced by other organisms

        •  they metabolize carbohydrates, lipids, proteins as a source of energy by hydrolysis

    • Different organisms use various reproductive strategies in response to energy availability

      •  plants produce pollen at times of the year when pollinators will be active

      •  animals reproduce in the spring and summer when food is available for offspring

      •  reproduction is triggered by a critical photo period -  photo. being the relative length of day and night

      •  example: grizzly bears mate between May and July

        •  females delay implantation of eggs in the uterus until November or December 

  • Poulatiion ecology

    • A population is comprised of individual organisms of the same species

      •  individuals interact with one another in complex way

      •  individuals within a population usually interbreed with one another rather than individuals from another population

    • Adaptations are related to obtaining and using energy in a particular environment

      •  the size of populations depends on availability of resources

        •  when food is less available the population decreases

          •  reproduction rates decrease in Offspring survivability decrees

      •  different species have adaptations that Aid in survival when energy availability changes

        •  example: storage of fat during winter, losing leaves or growing leaves, migrating

    • Factors that can affect population growth

      •  age at reproductive matur

      •  number offsprijg produced

      •  frequency of reproduction

      •  survivors of Offspring ability to reproductive maturity

    •  population growth formula

      • DN = change in population

      •  DT = change in time

      •  b = birth rate

      •  D = death rate

    • Reproduction without constraints results in exponential growth of population

      •  exponential growth refers to a sharp increase in the growth of a population

        •  occurs under ideal conditions when resources are abundant

      •  exponential growth equation

        • change in population over change in time equals population size times maximum per capital growth rate of population

  • Effect of density on populations 

    • Resource availability in an environment impacts population density

      •  population density is how close individuals within a population live near one another

        •  when abundance of food is available the population can become

        •  when food is limited that density may decrease

    •  limits to population growth are due to density dependent  and independentfactors

      •  density dependent factors are abiotic or biotic factors whose effect on population size relies on a population density

        •  competition for resources, territory, disease, predators 

      • Density independent factors are factors that affect the population size regardless of population density

        •  floods, Forest fires, pollution 

    • A population can produce a density of individuals that exceeds the system's resource availability

      •  a logistic growth model describes population growth that initially starts slowly immediately followed by exponential growth and ends up with a relatively stable maximum growth

      • Under certain conditions a population can temporarily exceed the carrying capacity

        •  limiting factors bring the population size back

        •  the population size fluctuates naturally at carrying capacity 

    • Population Dynamics can be represented using mathematical models

      •  logistic growth equation

  • Community ecology

    •  the structure of a community is described according to the composition and diversity of species

      •  community is a group of different species living together in the same location and interacting with one another

      •  communities are described based on species diversity and species composition

        •  species diversity is the variety of species and quantity of individuals in each species

        •  species composition refers to the identity of each species 

    • Simpsons diversity index

      •  

      • Is used to measure the biodiversity of a habitat

        •  the higher the index value the more diverse it is

        •  based on random samples of the environment 

    • Interactions among populations determine how they Access Energy and matter

      •  communities change over time depending on interactions between populations

        •  competition is an interaction that can affect how populations Access Energy and matter

          •  competition over food or habitats

        •  it can be positive negative or neutral

          •  positive interactions

            •  mutualism where both species benefit

            •  commensalism where one species benefits and the other is neutral

          •  negative interactions

            •  predator and pray where one species is a food source

            •  parasitism where one species benefits at the harm of another

          •  neutral interactions

            •  just simply two organisms living in the same environment not competing for anything

    • Relationships among interacting populations can be characterized by positive and negative effects and models

      •  Predator prey interactions

        •  and increase in Predator population occurs after an increase in pray population

        •  and increase in the Predator population will eventually cause a decrease in prey population

      •  a trophic Cascade refers to the negative effect the removal of or decrease in a key species has on other trophic levels

      •  Niche partitioning refers to the decrease in competition over limited resources between two similar species 

  • Biodiversity

    • Ecosystem diversity is related to its resilience to changes in the environment

      •  natural and artificial ecosystems with fewer component parts and with little diversity are less resilient to changes in the environment

      •  the diversity of species within an ecosystem May influence the long-term structure of an ecosystem

        •  less vulnerable to drastic structural changes when organisms are added or removed 

    • Abiotic factor (nonliving factors (wind/rain)) and biotic factors (living organisms in environment

      •  abiotic factors can help maintain ecosystem diversity

        •  climate, nutrient availability, light availability

      •  biotic factors help maintain ecosystems diversity

        •  producers maintain ecosystem diversity

          •  many populations depend on producers for food and habitat

        •  dominant Predators keep prey populations under control

          •  diverse diets 

    • The effects of keystone species are disproportionate relative to their abundance

      •  keystone species are species the community structure depends on

        •  when they are removed from the ecosystem the ecosystem collapses

          •  example predators that control the size of populations so when that keystone species is gone overpopulation depletes resources

  • Disruptions to ecosystems

    •  evolution is characterized by the change in genetic makeup of a population over time

      •  adaptation is a genetic variation of a trait that is favored by selection 

    • Invasive species affect ecosystem Dynamics

      •  invasive species-  is one that is not native to the specific area and harm the community it is introduced to

      • the introduction of an invasive species can be intentional or unintentional

      •  invasive species exploit new niche 

        • Out compete other organisms for resources 

    • The disruption of local and Global ecosystems changes over time

      •  habitat change can occur because of human activity

      •  human impact accelerates change at local and Global levels

        •  urbanization deforestation erosion Extinction pollution climate change disease 

    • Geological and meteorological activities can change ecosystems

      •   Examples:  large habitat disruptions, chemical disruptions, reproductive isolation