Biological Control Programs Study Notes

Biological Control Concepts

  • Various documents outline the concepts of biological control and its applications.

    • Cornell University: Overview of Biological Control Concepts.

    • State of Connecticut: Fact sheet on biological control concepts.

    • NPIC: Overview of Bacillus thuringiensis.

    • University of Kentucky Extension: Detailed information on Bacillus thuringiensis and its applications for pest control.

    • USDA APHIS: Information on stingless wasps as a biological control mechanism.

    • University of Maryland (UMD): Update on biological control strategies against lawn grubs.

Terminology

  • Predator: An insect or arthropod, like spiders, that consumes another insect (its prey).

  • Parasitoid: An insect that develops on or within another insect (the host), leading ultimately to the host's death.

  • Parasite: An insect that lives on another insect or animal without killing it.

  • Pathogens: Microorganisms including bacteria and nematodes that are lethal to insects.

  • Beneficials: Encompasses all of the above except for parasites; these organisms assist in pest control.

Major Categories of Biological Control

  • Three Primary Categories:

    1. Classical Biological Control: This involves introducing a predator, parasitoid, or pathogen from another nation to manage an invasive or introduced pest.

    2. Augmentation Biological Control: This entails releasing more beneficial organisms to mitigate the pest population.

    3. Conservation Biological Control: The focus here is on adjusting programs to promote ecosystem balance, such as employing selective treatments or providing alternative food sources.

Entomopathogens

  • Definition & Role: Microscopic organisms that infect insects, proliferate within them, and result in the pest's death.

  • Types of Pathogens Used in Biological Control: Bacteria, viruses, fungi, protozoans, and nematodes.

Bacteria

  • Bacillus thuringiensis (Bt): A soil bacteria with insecticidal properties; it has numerous strains that target specific pests:

    • Bt kurstaki: Targets Lepidoptera larvae, commonly sold under trade names like Dipel, Javelin, and Thuricide.

    • Bt tenebrionis: Effective against leaf beetles, sold as Novodor.

    • Bt galleriae: Specifically targets beetles and white grub control.

Assessment of GrubGONE

  • Evaluation Data (Lancaster, Ohio, 2011): A chart presenting the percentage of control of white grub populations using various formulations of GrubGONE, showing different application rates on specific dates with corresponding control efficacy.

  • Trade names for competitive products, such as GrubEx and Meridian, illustrate comparative effectiveness in controlling larval pests.

Overview of Biological Insecticides

  • GrubGONE: A biological insecticide targeting various turf and ornamental pests, including leaf-eating insects.

    • Active Ingredient: Consists mainly of Bacillus thuringiensis galleriae.

    • Note: GrubGONE demonstrated effective pest control without adversely impacting beneficial insects.

Bacillus thuringiensis Modes of Action

  • The mode of action of Bt involves the production of protein toxins known as delta-endotoxins, which impact the insect gut:

    • These endotoxins bind to the gut lining of insects and compromise the gut, leading to cessation of feeding and eventual death.

    • Younger larval hosts are more susceptible than older larvae, necessitating ingestion for effectiveness.

Gypsy Moth and Insect Pathogen Applications

  • Case Study: Gypsy Moth suppression project aimed at preventing host tree defoliation via targeted applications of biological agents.

    • Project Objectives: Maintain defoliation below 30% and reduce insect populations to below threshold levels for subsequent years.

Entomopathogenic Nematodes (EPNs)

  • Description: Unsegmented roundworms that serve as parasites to insects, often acting in conjunction with specific bacteria (e.g., Xenorhabdus).

  • Life Cycle: EPNs go through an adult, four juvenile stages, and an egg phase; the third stage juveniles are the infectious form.

Key EPN Species and Applications

  • Various strains of entomopathogenic nematodes target specific pests; for example:

    • Steinernema carpocapsae: Effective for borers, weevil larvae, etc.

    • Heterorhabditis spp.: Noted for efficacy against pests like white grubs.

  • Application Rates: Often recommended at 1 billion units per acre for effective pest control.

Limitations and Efficacy Considerations for EPNs

  • Efficacy is influenced by environmental factors:

    1. Desiccation: Soil must remain moist during and after application.

    2. Sunlight Exposure: Increased mortality rates due to UV degradation.

    3. Temperature Extremes: Ideal conditions range between 50-85°F.

Fungal Pathogens Overview

  • Fungi can naturally occur in soils and are used globally in agricultural pest management.

    • Efficacy relies on environmental conditions during application; high humidity and temperatures are vital for effectiveness.

Popular Fungal Pathogens Used in Control

  • Beauveria bassiana and Metarhizium spp.: Established fungal pathogens; application can lead to significant pest mortality by overwhelming insect cuticles, causing desiccation.

    • Examples include using Beauveria for controlling billbug infestations and utilizing Metarhizium for managing white grub populations.

Viral Pathogens

  • Defined as insect-specific viruses that often face challenges due to high production costs and host specificity:

    • Examples of forest pest control using viruses are Gypcheck® for Gypsy Moths and TM BioControl-1® for Douglas-fir tussock moth.

Summary and Conclusion

  • The biological control landscape includes a diverse array of strategies and applications, focusing primarily on integrated pest management using a variety of organisms (microorganisms, pathogens) to suppress pest populations effectively.