Cell Motility and Chemotaxis Notes

Cell Motility and Chemotaxis

  • Importance of cell motility and chemotaxis:
    • Development
    • Wound healing
    • Infection
    • Neuronal pathfinding
  • Mechanisms:
    • Actin-based cell motility and chemotaxis
    • Microtubule-based cell motility and chemotaxis

Overview of Cell Movement

  • External Signals:
    • Soluble factors
    • Interactions with other cells
    • Extracellular matrix components
  • These signals trigger signal transduction pathways within the cell's cytosol, ultimately affecting the cytoskeleton.
  • Cytoskeleton's Role:
    • Organization and movement of organelles
    • Cell shape, overall movement, and contraction

Chemotaxis

  • Chemotaxis is the ability of a cell to sense a directional signal and move towards or away from it.
  • Dictyostelium discoideum:
    • Example of chemotaxis, aggregating in response to cAMP.
    • Life cycle: Free-living amoeba reproduce, aggregate in response to cAMP, form a motile slug, and eventually a fruiting body with spores.

Importance of Chemotaxis in the Human Body

  • Directed cell movements during development
    • Gastrulation
    • Neural crest migration
    • Primordial germ cell migration
  • Immune surveillance
    • Phagocytosis of pathogens
  • Inflammatory response to injury
    • Lymphocyte migration
  • Wound healing
    • Fibroblast migration

Neutrophil Chemotaxis Example

  • Neutrophils chase, catch, and phagocytose bacteria.
  • Bacteria secrete fMet-Leu-Phe (fMLP), a tri-peptide.
  • fMLP is sensed by receptors on the neutrophil plasma membrane.
  • Neutrophils