PCR + Electrophoresis

PCR

There’s a target sequence in the Dna that we’re trying copy

  • the target sequence is a gene
  • creates a large amount of copies
  1. Denature

   

  1. done at 94 degrees Celsius
  2. done to pull apart the the Dna strands
    1. Annealing

   

  1. done at 40-65 degree Celsius
  2. The stage where the primer attaches to the strands if dna

       1. identifies the start and the end of the target sequence

  1. Extension

   

  1. occurs at 72 degrees Celsius
  2. stage where taq polymerase attaches to the strands

       1. taq polymerase builds the dna compliment of target sequence using the dnpt’s

          1. dnpts are the free dna nucleotides

  1. Thermocyler

   

  1. the machine used for pcr

Electrophoresis

  1. Pouring the gel
  2. 2) Preparing your samples
  3. 3) Loading the gel, 4) Running the gel (exposing it to an electric field)

   

  1. Dna is negative because of the sugar phosphate backbone it has
  2. \
    1. 5) Staining the gel.