Wildlife Diseases Notes

Wildlife Diseases

  • Wildlife diseases can affect humans and domestic livestock, impact wildlife populations, and indicate environmental issues like overpopulation.

Why We Care About Wildlife Diseases

  • Zoonoses: Wildlife carries diseases that can affect humans, such as rabies, West Nile Virus, and avian influenza.

  • Impact on Livestock: Wildlife can transmit diseases to domestic livestock, including bovine tuberculosis and pseudorabies.

  • Effects on Wildlife Populations:

    • Game species: Diseases like anthrax (in deer) and avian cholera (in waterfowl) can affect game species.

    • Threatened/Endangered (T/E) species: Canine distemper can impact T/E species.

  • Overpopulation Symptoms: Diseases and conditions like deer malnutrition/parasitism syndrome can be indicative of overpopulation.

Vocabulary

  • Pathogen: A disease-causing agent.

  • Zoonotic: A human disease that can be carried by animals.

  • Reservoir: A pool of infected animals that transmit pathogens to others, often without showing symptoms themselves.

  • Vector: An animal (often an arthropod) that transmits pathogens among wildlife.

  • Epidemiology: The study of pathogens and their progress through a population.

  • Incubation Period: The variable length of time between infection with a pathogen and the appearance of clinical effects.

  • Endemic: An area where a disease occurs naturally or regularly.

Pathogens

  • Prions:

    • Aberrant proteins that can spread within and between animals.

    • Cause transmissible spongiform encephalopathies (TSEs).

  • Viruses:

    • Packets of DNA or RNA with a protein coat.

    • Require host cells to reproduce.

  • Bacteria:

    • Prokaryotic, unicellular organisms.

    • Can be pathogenic or benign.

  • Protozoans:

    • Eukaryotic, unicellular organisms.

    • Can be pathogenic or benign.

  • Internal Parasites:

    • Mostly worms, such as tapeworms and roundworms.

  • External Parasites:

    • Mostly arthropods, such as insects, ticks, and mites.

Transmissible Spongiform Encephalopathies (TSEs)

  • Caused by misfolded prions.

  • Examples:

    • Scrapie

    • Bovine Spongiform Encephalopathy (BSE; "Mad Cow Disease")

    • Creutzfeldt-Jakob disease (CJD)

    • Variant Creutzfeldt-Jakob disease (vCJD)

    • Kuru

    • Chronic Wasting Disease (CWD)

Prions

  • Very resistant to destruction.

    • Boiling, autoclaving, heat sterilization, and cooking do not denature prions.

    • Irradiation does not denature prions.

    • Can be denatured/destroyed with bleach, acid baths, and protease treatments.

  • TSEs/Prions may be aerosolized and transmitted via bodily liquids.

  • Prions from some TSEs (CWD) may persist in the soil for decades.

  • Smaller than the smallest known virus by 100 times.

  • Do not contain nucleic acids (DNA or RNA), so vaccines are ineffective.

  • Can reproduce/repeat on their own, becoming infectious and transmissible.

  • Two general types:

    • PrP-sen: "sensitive"; normal (not misfolded).

    • PrP-res: "resistant"; resistant to being broken down and is the disease-causing form.

    • When PrP-sen comes into direct contact with PrP-res, they are converted to PrP-res, causing a chain reaction.

Misfolding Prions and Clinical Signs

  • PrP-res proteins stick together and form long chains called "amyloid fibers."

  • Amyloid fibers are toxic to cells and kill them.

  • Cells called Astrocytes digest dead neurons, leaving holes and amyloid fibers in the brain.

  • Causes encephalopathy, hence Transmissible Spongiform Encephalopathy (TSE).

Organs Affected by Specific TSEs

  • Thalamus: Fatal Familial Insomnia

  • Cerebral cortex: Classic CJD

  • Brain stem: Scrapie, BSE, and Chronic Wasting Disease

  • Cerebellum: Kuru

Organs Affected by Chronic Wasting Disease (CWD)

  • Brain: Prions attack neurons, causing degeneration, teeth grinding, drooping head, muscle weakness, and weight loss.

  • Eyes: Prions accumulate in the soft matter.

  • Spinal Cord: Prions can accumulate, causing weakness, abnormal behavior, and loss of coordination.

  • Spleen: Prion accumulation occurs.

  • Lymph Nodes: Accumulation erodes the immune system.

Scrapie

  • Fatal, degenerative TSE disease affecting sheep and goats.

  • First recognized over 250 years ago in Great Britain and Western Europe.

  • Reported throughout the world.

  • Largely under control.

  • In 2022, 21,600 samples collected, 491 positives.

  • In 2023, 10,000+ collected, no positives.

Bovine Spongiform Encephalopathy (BSE; Mad Cow Disease)

  • Progressive neurological disorder (TSE) of cattle caused by a prion.

  • First infections occurred in the 1970s.

  • Likely originated from feeding cattle meat-and-bone meal containing BSE-infected products from spontaneous BSE cases or scrapie-infected sheep products.

  • Spread throughout the UK cattle industry by feeding rendered, prion-infected, bovine meat-and-bone meal to young calves.

Mad Cow Disease (BSE) Epizootic in the United Kingdom

  • Peaked in January 1993 at almost 1,000 new cases per week.

  • Annual numbers of BSE cases in the UK have since dropped:

    • 2 cases in 2015

    • 11 cases in 2010

    • 225 cases in 2005

    • 1,443 cases in 2000

    • 14,562 cases in 1995

  • Cumulatively, through the end of 2015, more than 184,500 cases of BSE had been confirmed in the UK in over 35,000 herds.

  • Current Control in the US:

    • Severe restrictions on the importation of live ruminants (cattle, sheep, and goats) and certain ruminant products from countries where BSE was known to exist.

Creutzfeldt-Jakob Disease (CJD)

  • Several rare fatal diseases of humans are also TSEs.

  • CJD occurs worldwide.

  • Three major categories of CJD:

    • Sporadic CJD: No known risk factors. Most common type (at least 85% of cases).

    • Hereditary CJD: Family history and/or positive genetic mutation test (5-10% of cases in the US).

    • Acquired CJD: Transmitted by exposure to brain or nervous system tissue, usually through medical procedures (<1% of cases since 1920).

  • vCJD (variant CJD): Discovered in 1996, thought to have resulted from the BSE (mad cow) outbreak in Europe.

  • Abnormal prion proteins aggregate, leading to neuron loss and other brain damage.

Kuru

  • A very rare disease, a TSE.

  • Caused by an infectious protein (prion) found in contaminated human brain tissue.

  • Causes brain and nervous system changes similar to Creutzfeldt-Jakob disease.

  • Found among people from New Guinea who practiced cannibalism, eating the brains of dead people as part of a funeral ritual.

  • Practice stopped in 1960.

  • Cases reported for many years afterward due to the long incubation period (up to 50 years).

  • Was the start of an epidemic of kuru among the Fore people; at its peak in the 1950s, killed up to 2% of the tribe each year, with 1000 concurrent cases in PNG in 1957.

  • Epidemiological surveillance and studies on human genetics and past mortuary practices are ongoing in the kuru-affected region.

Chronic Wasting Disease (CWD)

  • Transmissible spongiform encephalopathy (TSE) disease that affects deer and elk.

  • Similar to Mad Cow disease in cattle and scrapie in sheep.

  • No evidence CWD affects humans…yet….

  • Fatal, with a long incubation time (>1 year).

  • Generalized wasting syndrome.

  • Endemic to Colorado/Wyoming but recently spread to WI, NY, TX, TN, AL, etc.

Species Susceptible to CWD (as of 2024)

  • Mule deer

  • Black-tailed deer

  • White-tailed deer

  • Elk

  • Moose

  • Reindeer/caribou

  • Red deer

  • Sika deer

CWD Spread

  • 28 states & 4 Canadian provinces.

  • Still rapidly expanding.

  • Newest detection: Alabama in early January 2022.

CWD Transmission and Environmental Factors

  • Shedding:

    • Nasal secretions

    • Saliva

    • Antler velvet

    • Skin

    • Blood (open wound/lesion)

    • Birthing matter

    • Milk

    • Urine and feces

  • Agent Transport:

    • Cervid host movement

    • Water

    • Dust

    • Scavengers

    • Predators

    • Insects

  • Environmental Hot Spots:

    • Carcasses

    • Scrapes/rubs

    • Mineral licks

    • Wintering areas

    • Captive facilities

  • Soil Binding:

    • Immobilization

    • Altered infectivity

    • Altered stability

    • Variance with soil type

  • Environmental Degradation:

    • Mineral oxidation

    • Heat/dessication

    • Freeze/thaw

    • Biodegradation

  • Uptake:

    • Direct contact: Saliva/mucus

    • Indirect contact: Soil/water/vegetation/surfaces

    • Ruminant gut passage

    • Intestinal uptake

    • Oral ingestion

    • Oral lesion uptake

    • Nasal inhalation and uptake

CWD Signs and Symptoms

  • Severe emaciation & dehydration.

  • Loss of coordination, drooping of heads/ears, excessive salivation.

  • Long incubation time.

  • Ultimately fatal!

CWD in Texas

  • First case discovered in 2012 in free-ranging mule deer in West Texas.

  • First case in Texas white-tailed deer was found in a deer-breeding facility in 2015 during routine disease monitoring.

  • Map of positives (2018).

  • 3 species found positive (white-tailed deer, mule deer, elk).

  • Texas has recorded 500+ confirmed cases, mostly discovered in captive deer breeding pens.

  • The Texas Parks and Wildlife (TPW) Commission adopted comprehensive CWD Management Rules on June 20, 2016.

  • Many other legislative actions have since taken place.

CWD Complications in Texas

  • Urban areas: human-wildlife conflict emerges.

  • Hunters.

    • 30,000 Mule Deer; 757,000 White-tailed Deer (predicted 2023-2024)

  • Deer breeders.

    • 980 deer breeders in Texas (September 2021).

    • $1.6 billion annual economic impact (2017).

    • https://tpwd.texas.gov/business/permits/land/wildlifemanagement/deerbreeder/

Texas Parks and Wildlife (TPWD) CWD Epidemiology

  • Amplification Factors:

    • Movement

    • Density

    • Cumulative contamination

  • Domestic Cycle

    • CWD susceptible species movement

    • Contaminated Feed?

    • Veterinary practices?

    • Reproductive practices?

  • Sylvatic Cycle

    • Environmental Contamination

    • Escapes

    • Fence line contact

    • Run-off

    • Scavengers

    • Invertebrates

Regulatory Authority for CWD in Texas

  • CWD is a reportable disease.

  • Texas Animal Health Commission (TAHC) has authority for reporting and tracking the disease in alternative livestock (elk, red deer, and sika deer).

  • TPWD has regulatory authority for free-ranging white-tailed deer and mule deer.

  • Both agencies share regulatory authority over captive deer held under Deer Breeder Permits.

  • Deer are owned by the public by law, but Texas is unique in that “private” practices are performed on a “public” entity.

Challenges with CWD Regulations

  • Recent cases where captive facilities are depopulated by TPWD as a disease management strategy.

  • Litigation and lawsuits from private citizens suing TPWD, the state of Texas, or individual employees.

Epizootic Hemorrhagic Disease (EHD) and Bluetongue Virus (BT)

  • Viral diseases affecting deer, sheep, and pronghorn.

  • Same symptoms, different diseases.

  • Most important disease affecting deer in most areas.

  • Causes rapid disease and up to 60% mortality.

  • Hemorrhage in many tissues (like Ebola in humans).

  • Endemic to the south, with periodic outbreaks farther north.

  • Southern deer can be resistant, especially in FL and TX.

Hemorrhagic Disease and Bluetongue

  • Causative agent(s): Epizootic hemorrhagic disease (EHD) viruses (2 serotypes) or bluetongue (BT) virus (5 serotypes).

  • Hosts: White-tailed deer, mule deer, pronghorn, elk, bighorn sheep, etc.

  • Signs & symptoms: Highly variable; swelling of the head/tongue/neck; fever; respiratory stress; reduced activity; often found near water sources.

Rabies

  • Viral disease affecting all mammals, including humans.

  • Acute neural disease that is always fatal.

  • Affects brain function; animals may be lethargic or manic (attacking anything).

  • Most important carriers in the U.S. are raccoons, foxes, skunks, and bats.

  • Raccoon rabies is spreading in the east, with a large effort to stop it.

Avian Cholera

  • Bacterial disease affecting waterfowl and some other birds.

  • Rapidly fatal in ducks and geese.

  • Rarely see sick birds, just dead ones.

  • Birds often in good body condition.

  • May decimate populations in concentrated areas (wintering or stopover grounds).

  • Signs include lethargy, convulsions, many dead birds very suddenly.

  • Hemorrhage on the heart and gizzard, spots on the liver.

Avian Botulism

  • Occurs naturally and is a leading cause of bird death, especially in migratory waterfowl.

  • Millions of birds die annually worldwide.

  • Caused when a virus infects type C Clostridium botulinum bacteria, causing the bacteria to produce a toxin.

  • The botulism spore, or resting stage of the bacteria, is commonly found in wetland soils and can survive for years, withstanding freezing and drying.

Avian Influenza (Bird Flu)

  • Refers to the disease caused by infection with avian (bird) influenza (flu) Type A viruses.

  • These viruses naturally spread among wild aquatic birds worldwide and can infect domestic poultry and other bird and animal species.

  • Virus is present in their saliva, nasal secretions, and feces.

  • Susceptible birds become infected when they have contact with the virus as it is shed by infected birds.

  • Infection can also occur through contact with surfaces contaminated with the virus from infected birds.

  • Bird flu viruses do not normally infect humans, but sporadic human infections have occurred.

  • There is a growing number of cases. Likely the largest wild bird disease issue right now as of 2022.

Lyme Disease

  • Zoonotic disease caused by a bacterium.

  • Does not affect wildlife.

  • Causes flu-like symptoms and chronic joint pain in humans.

  • Vector is the black-legged (deer) tick.

  • Reservoir for the disease is in white-footed and deer mice.

  • Deer are hosts for the tick vector, not the bacterium.

Mange

  • Skin disease of wild canids caused by mites.

  • Especially severe in red foxes and coyotes.

  • Causes extensive hair loss, foul odor, and inflammation of the skin.

  • Death is possible due to secondary infection, emaciation, or hypothermia.

  • Can cause temporary skin irritation in humans.

Ranavirus

  • Ranaviruses infect amphibians, reptiles, and fish; however, susceptibility varies by species and across phylogenetic lineages.

  • For amphibians, the orders Anura and Caudata are affected, and natural infections have been documented in at least 72 species.

  • Ranaviruses have also been associated with disease in snakes, lizards, and chelonians and may be best known for die-offs in eastern box turtles.

  • Transmission is horizontal via direct contact, ingestion of the virus or infected animals, and water exposure.

  • Studies have shown that infection can begin within seconds of contact.

  • Disease (and deaths) may be established in as short as 3 days but also may take weeks and is likely dependent on host susceptibility.

White-Nose Syndrome (WNS)

  • The fungal disease killing bats in North America.

  • Research indicates the fungus that causes WNS is likely exotic, introduced from Europe.

  • Started in New York in 2006 and has spread to more than half of the United States and five Canadian provinces by August 2016.

  • Scientists predict some regional extinctions of bat species.

West Nile Virus (WNV)

  • The leading cause of mosquito-borne disease in the continental United States.

  • Most people infected with WNV do not feel sick.

    • 1 in 5 humans develop a fever and other symptoms.

    • 1 out of 150 humans develop a serious, sometimes fatal, illness.

  • It infects over 250 species of birds, but corvids (crows, blue jays, and ravens) are the most susceptible to dying from the disease.

  • Although tens of thousands of birds have died, and studies of some bird species show regional declines, few region-wide declines can be attributed to WNV.

  • Horses represent approximately 97% of all reported non-human mammalian cases of WNV.

  • Very little is known about other mammals.

Bot Fly Infestation

  • Parasitic larva of bot flies infect many species.

  • Generally does not cause significant harm.

  • Unsightly, leading to wasted meat.

  • Infect the skin of squirrels (warbles, wolves), rabbits, and small mammals.

  • Infect nasal passages of white-tailed deer.

Cutaneous Fibromas

  • More commonly called deer warts by hunters, are occasionally observed on harvested deer.

  • The fibromas are peeling, especially when they appear in large numbers.

  • "Grotesque" is a common description of these harmless skin blemishes.

  • Cannot be transferred to humans.

  • Very rarely cause harm to deer, only when disabling movement, eyesight, smell, etc.

Canine Distemper

  • A contagious and serious disease caused by a virus that attacks the respiratory, gastrointestinal, and nervous systems of carnivores.

  • Broad host range among North American carnivores, including mink, striped skunks, fishers, red and gray foxes, raccoons, coyotes, and weasels.

  • Most often become infected through airborne exposure (through sneezing or coughing) to the virus from an infected animal, but urine, saliva, etc., are also responsible.

  • Infected animals will develop watery to pus-like discharge from their eyes. They then develop fever, nasal discharge, coughing, lethargy, reduced appetite, and vomiting.

  • There is no cure, only supportive care, so preventative measures to reduce the risk of spreading the virus are key. This virus is nearly 100% fatal for mustelids.

  • Outbreaks of canine distemper may have a significant impact on local or regional populations of raccoons, gray foxes, and skunks in parts of the United States and on raccoons in Canada.