Life: Notes
Science and Scientific Methodologies
Learning objectives:
1) Explain what science is and its role in human thought
2) Describe and explain the three different ways of scientific reasoning: Deduction, Induction, and Abduction
3) Distinguish and apply those modes of reasoning in a scientific investigation
4) Recognize valid scientific questions in different areas of knowledge
Goal of science
Establishment of general laws/ theories
Adequate description of things and events
Events may be explained and understood
Describe our world and understand the causes of what we observe in the world
Types of questions
What?
Description/ identity
What is this thing?
Why?
Cause/ explanation
Why did this happen?
How?
Function/ quantity
How does this thing work?
If you want to know something, you need to know what to ask.
Fact
An observation that has been repeatedly confirmed.
Hypothesis
A tentative statement about the natural world leading to deductions that can be tested.
Theory
A well established explanation of the natural world that is based on tested hypotheses.
Law
Statements based on repeated experiments or observations that describe a range of natural phenomena.
The scientific method/ process
Observation/ question -> Research topic area -> Hypothesis -> Test with experiment -> Analyze data -> Report conclusions-> repeat.
There is no ONE scientific method, but there ARE different scientific methodologies.
Deductive
Non- deductive
Inductive
Abductive
Deductive
Consider a set of premises or hypotheses
Draw a logical conclusion from those
Example: “All insects have 6 legs.” -> “The grasshopper is an insect” -> The grasshopper has 6 legs.
BUT… The method is unable to verify those premises. (It is assumed to be true.) Falsehoods can be concluded, even if they are logical.
Example: “All snakes are venomous” -> “Black widows are venomous” -> Black widows are snakes.
Inductive
Aim for broad generalizations based on specific observations
Use the available data to draw the best conclusion possible from the data
Aristotle said that knowledge building is primordially a SENSORY EXPERIENCE.
General laws are inferred from particular facts, a guess or tentative explanation
Problems with inductivism
Just because something happens often doesn’t mean it's guaranteed to continue to happen.
“Past does not predict future”
Biases on data collection
“Just because you never see a black swan, doesn’t mean it doesn’t exist”
Hypothetico- deductive method
A hypothesis is formed by the first two steps of inductive reasoning
Hypothesis is tested by validating or falsifying a prediction
If the hypothesis is true, the deduction must be too.
Deduction is tested with experiments/ observations
If deduction is false, the hypothesis is falsified.
Abductive method
Based on limited or incomplete explanation.
Looks for the most likely explanation, to the data, given previous knowledge.
We use abduction to produce theories and hypotheses.
We use deduction to predict potential test evidence.
We use induction in the act of testing those hypotheses.
Only statements from which a testable prediction can be made into a scientific process.
Error and biases
Learning objectives:
1) Identify and explain the nature and sources of error in experiments
2) Distinguish between Accuracy and Precision and the implications of those qualities in science
3) Develop basic proficiency in the usage of spreadsheets to do basic statistical calculations
4) Explain what biases are and how they can mislead your thinking on a scientific pursuit
Science and uncertainty
Uncertain -> it can change, like any human activity.
Science can be something we don’t know.
Scientists are aware of those uncertainties & try to control them.
Without acknowledging those, scientific results are meaningless.
Measurements
Property of something
How heavy, tall, hot, long, etc.
Using an instrument
Ruler, weighing scales, stopwatches, etc.
Uncertainty: “Give or take” -> difficult to be exact.
Example: 37.58 (magnitude/ value) + / - 0.01 (uncertainty/ error) cm (unity)
What is error?
A basic assumption in any scientific endeavor that no measure is correct.
Error is the differences between observed/ measured values.
Error ≠ uncertainty
Accuracy
Trueness of an answer/ measure
How correct or close to the true answer or measure is.
Precision
Refers to the spread of the values
How close to each other your measures/ answers are.
We can guarantee precision, not accuracy.
Why precision?
Repeatability and falsifiability
No scientific hypotheses/ experiment can guarantee absolute accuracy (truth) of its content, but all of them have to guarantee precision.
Two main kinds of scientific errors
Random (or indetermined)
Causes: Observers’s judgment, unpredictable fluctuations
Effects: Affects precision- how close answers are to each other.
Mitigation: Cannot be minimized, but it can be changed in experimentation.
Systemic (determined)
Causes: Consistent and repeatable causes that shift all measurements in the same directions.
Effects: Affects accuracy- how close to the truth.
Mitigation: The whole experiment must be restarted and measurements discarded.
Mathematics and errors
Standard deviation
Formula
No two measurements will be the same, especially in biology.
There can be outliers.
Outliers can impact averages and calculations that lead to false conclusions. It’s important to correct for outliers.
Statistical treatment that allows access to the spread of your data.
Is the variation of data random or is the differences of the data significant?
Standard error
Formula:
Standard deviation of a particular distribution and estimates how close the sample mean is to the true mean.
It allows us to compare different sets of data collected from the same sample.
Mean
Average (data range)
Standard deviation
STDEV (data range)
Standard Error
STDEV (data range)/SQRT (count(data range))
Biases
Definition: A systematic distortion that causes error in different ways.
Can and most likely will falsify your data.
Can get to the right answer for the wrong reasons.
Biases come from…
Context
Personal beliefs
Preconceptions
Skewed information sources
Problems with biases…
We don’t realize we are biased.
Some are shared by such a big group of people which makes it harder to get rid of them.
Science is made by humans so it is imperfect!
What is life?
Learning objectives:
1) Explain why "life" is a difficult concept to determine
2) Describe and explain the characteristics of life as used in Science
3) Distinguish between living and nonliving things according to science
4) Defend a position on a two-sided matter using reputable sources and evidence-based arguments
Biology = The science of life.
Life is a process, not a substance.
Theological
Philosophical
Legal
Scientific
Scientific definitions describe life, rather than define it.
7 characteristics of living things
Order/ organization
All lifeforms are ordered & structured into small unities.
Reproduction/ replication
All lifeforms can produce new organisms (heredity)
Growth/ development
All lifeforms increase in size in all their organizational units (develop/ mature)
Sensitivity
All lifeforms can perceive intrinsically (its own conditions) and extrinsically (the environment that surrounds it).
Energy process
All lifeforms can acquire and use energy to fuel their multiple processes.
Regulation
All lifeforms can regulate their internal environment to a constant state (homeostasis)
Metabolism, anabolism, catabolism
Adaptation/ evolution
All lifeforms are capable of change over time to better survive in its environment.
The Species Problem
Learning objectives:
1) Explain the "species problem" and its effects on biodiversity understanding
2) Describe the different species concepts
3) Analyze and evaluate different species concepts, pointing out their strengths and weaknesses
4) Define the Hardy-Weinberg principle and discuss its importance (Readings)
5) Explain the four main processes that lead to Evolution: Gene drift; Gene Flow/ migration; Mutations; and Natural Selection (Readings)
Species
From latin = appearance, quality
Some usages
Type/ kind
Image/ appearance
Coin or coined silver or other metal, currency
In biology…
Hard to define (the species problem)
Biological species concept
A species is a group of actually or potentially interbreeding natural populations, which are unable to successfully mate or reproduce with other such groups.
Problems with that concept
Interbreeding
Not always straightforward (fossils) or possible t o observe (dinosaurs)
Asexual reproducing beings
Hybridization (Hybrid = offspring of crosses between different species)
Ring species
A chain of animal groups that live in a circle, where each group can have babies with its closest neighbors, but the two groups at the very ends of the chain are too different to breed together.
Scientists want
Universiability
Apply to as many types of organisms as possible
Applicability
Applicable in all circumstances
Theoretically significant
Describe real events to further understanding
Morphological species concept:
A species is a set of individuals with morphological features in common. (Similarity)
Problems
Subjective
Outliers
Sibling species
Closely related species with no gene exchange but nearly indistinguishable
Intraspecific variation
Species vary so much that they should be considered a different species, but they live in the same area & interbreed.
Sexual dimorphism
Males/ females or juveniles/ adults look very different, but they are members of the same species. However, they are sometimes identified as different species.
Ecological species concept
A species is a set of organisms adapted to a particular set of resources- called a niche in the environment.
Problems
“Niche” is hard to define objectively
If two or more organisms' niches intersect, are they the same species?
Genetic species concept
A species is a set of individuals that share 98% or more of similarity in a particular gene.
Problems
“Random” percentage for similarity
Phylogenetic species concept
A species is the smallest group of organisms that has at least one diagnostic character.
Problems
Are differences relevant?
Philosophical questions
Does biodiversity really fall into discrete units we can call a species?
Or are species a man-made artificial category?
Natural kinds
Natural
Natural grouping, not artificial, specific properties that distinguish from other groups.
Species Problem
Are species a natural kind? Or are they just abstract constructs of human rationale?
Single sort of species or many sorts?
Studying life: Systematics
Learning objectives:
1) Distinguish between the main branches of systematics: phylogenetics and taxonomy
2) Describe and explain the main elements of a phylogenetic tree and how to extract information from it
3) Explain the LUCA concept and the evidence that supports it.
4) Differentiate between monophyletic, paraphyletic, and polyphyletic groups; homology and analogy; convergence and divergence
5) Explain the Linnaean classification system, connect it to the modern understanding of evolution, discuss its importance, and be able to criticize grouping as being modern or not.
6) Explain how systematics are scientific endeavors and how repeatability and falsifiability are possible on phylogenies and taxonomic hypotheses.
Systematics= The field of biology that comparatively studies features of organisms and the relationship among themselves and conveys this information in a meaningful way.
Goals
Phylogenetics
To reconstruct the pattern of ancestor to descendant species through time.
Taxonomy
To name and describe life
Natural selection= non-random
Gene drift= random
Gene flow/ migration= random
Mutations= random
Uncovering evolution steps: Phylogenetics
Phylogeny
A depiction of ancestor- descendant relationships between 2 or more organisms. Usually shown in a tree.
LUCA
“Last Universal Common Ancestor”
Single-cell free-living organism
Metabolize hydrogen
Extremophile (acid/ hot)
Evidence for LUCA
All living organisms we know…
Use RNA & DNA as genetic material
Use the same 20 L-amino acids to build up proteins and D-sugars to process energy
Have ribosomes
Have a lipid bilayer cell membrane
ATP synthase
A phylogenetic tree = the hypothesis of relationships among different taxa
Observable data
Can be falsified
Evidence used to construct phylogenetic hypothesis
Characters
Observable properties or attributes of a given organism
Can be divided into character states
Observable variations of those attributes
Data we can use
Sources of characters:
Anatomy
Fossil record
Behaviors that have a genetic basis
Ontogeny and development
Molecular information
Different reasons for look alike
Homology
Similarity due to common ancestry, divergent evolution
Analogy
Similarity due to similar selective pressures, convergent evolution
How do we tell them apart?
Morphological similarity
Resemblance in detail
Topological similarity
Similar preposition in relation to neighboring structures or organs
Ontogenetic similarity
Similarity in embryological development
Congruence
Agreement with the rest of characters story
Monophyletic group
A group of taxa that encompasses all the derivatives from the same node.
Paraphyletic taxa
A group of taxa that encompasses only some of the derivatives of the same node.
Polyphyletic taxa
A group of taxa that encompasses derivatives from multiple nodes.
Taxonomy: Naming and describing life
Binomial Nomenclature
A system of two part names: Genus and species name
Species name: italized
Hierarchical Classification
System is organized in a series of categories
Mutually exclusive and subordinate
Modern Classification
Natural groups
Reflect natural or monophyletic groups
The Origins of Life
Learning objectives:
1) Describe the elements of life and where and how they were present on Early Earth
2) Explain what abiogenesis means in scientific terms and differentiate it from non-scientific uses
3) Explain the different hypotheses for the origin of life, their strengths, and their gaps
4) Explain the importance of the Miller-Urey experiment.
4) Follow the chronological steps and explain the challenges of life from inorganic compounds to the first protocells.
Geologic Time Scale
Divided into 4 eons.
Both eons are divided into eras.
Eras are divided into periods.
Periods are divided into epochs.
Ga (Giga annum) = 1 billion years Ma (Mega annum) = 1 million years Ka (Kilo annum) = 1 thousand years
The SuperEon Precambrian
Makes up for about 7/8ths of the Earth’s history: From the formation of the planet
Key events
Origin of life
The origin of eukaryotic cells
The appearance of multicellular organisms
Early Earth’s environment
Temp was VERY hot
Volcanic activity
Asteroid bombardment
Late Heavy Bombardment
Marked by a constant bombardment of meteors.
Moon formation -> impact of a theia (a Mars sized planetoid)
Effects
Changed the temp of the planet
Changed composition of atmosphere
Delivered rare but necessary life elements
Example: Phosphates
High energy released could have also delayed the origin of life
Archean (4.2-5 billion years ago)
Marked by a severe diminishing of the bombardment and the first emergence of life
Stromatolite
Sedimentary formations created (3.4 billion years ago)
CHNOPs- the elements of life
Carbon, hydrogen, nitrogen, oxygen, phosphorus, sulfur
Abiogenesis (1920s)
Life originated from non-living compounds
Consists of: Proteins, lipids, nucleic acids, carbohydrates
The atmosphere consisted of…
Water
Carbon dioxide
Nitrogen gas
Hydrogen
Methane
Hydrogen sulfide
Hypotheses of Origins of Life
Primordial Soup
Chemically reducing atmosphere
Produce the first organic monomers
Accumulated to make a “soup” to create more complex compounds.
Problems
Fewer of the reducing molecules
Way more CO2, very little CH4
Hydrothermal vents
Cracks in the ocean crust create a gradient in positively charged protons that serve as a “battery” to fuel creation of organic molecules and proto-cells.
Panspermia
Life is widespread throughout the universe and can colonize different planets.
Problems
Doesn’t answer question
Hard to test
Molecular panspermia
Small organic molecules used in the early stages of life might have originated extra-terrestrically
Supported by real evidence
Metabolism First
Small chemical reactions would occur in early Earth
Release free energy that would power more complex molecules
Replication First
RNA came before DNA
RNA can replicate itself & catalyze reactions
Cell Membranes
Lipid bilayer -> forms sphere around cell contents
The History of Life
LUCA came to be 3.5-3.8 BA
Rise of Early Prokaryotes
First single-cell organism
DNA loose in the cytoplasm as a circular loop
Outer cell membrane surrounds cell wall
Cyanobacteria and photosynthesis
Pigments
Ability to use sunlight instead of thermal vents as a source of energy (phototrophy)
Proterozoic( 2.5 BA- 538 MA)
Great oxidation event (2.3-2.7 BA)
O2 began accumulating in the atmosphere and rusting iron rich rocks.
Eliminated many lifeforms
Consequences
Allowed for oxidation and oxidative processes, blocking UV light
Solved energy process- independence of inorganic forms of energy
Changed composition of atmosphere, diminishing CH$, increasing CO2 and H2O
Reduced GHG effect
Rise of Eukaryotes (1.65-2.2 BA)
Larger and more complex than prokaryotes
Nucleus that holds genetic material, wrapped in chromosomes
Membrane bound organelles
Endosymbiosis
The idea that some eukaryotic cell organelles were once free living organisms.
Unicellular vs. Multicellular
Unicellular
All life functions carried out within a single cell
Multicellular
All lifeforms function with many cells.
Advantages:
Division of labor
Larger body size
Store more nutrients
Ediacaran Biota (541-635 BA)
Unique body composition
Lacked mouthparts
Soft-bodied organisms preserved worldwide as fossil impressions in sandstone
The History of Life
Learning objectives:
1) Describe the characteristics of prokaryotic and eukaryotic cells, differentiate between them, and explain the evolutionary significance of those characteristics
2) Differentiate between the two major Endosymbiotic theories (Symbiogenesis and Inside-Out hypotheses), their strengths, and gaps.
3) Identify the major steps in the evolution of life on Earth; organize them in chronological order and demonstrate how each step depended on the previous.
4) Summarize the major extinction events, identify commonalities amongst them, and correlate them to our present situation
The Last Eon: The Phanerozoic
Periods:
Paleozoic
251-541 MA -> Cambrian (485-541 MA)
Mesozoic
66-251 MA
Cenozoic
66 MA - present
Cambrian Era
Rise of bones (unique to vertebrates)
Ordovician (443.8-485.4 MA)
Increased marine fauna by x4
Skull and backbone development which helps with protection + locomotion
Colonization of land (470 MA)
Life began to move to land
Life was restricted to water before this
Lichen was one of the first on land organisms as well as algae
1st mass extinction- Late Ordovician (444-447 MA)
Killed 85% of species
Due to global warming, volcanism, anoxia (lack of oxygen)
Silurian (419.2-443.8 MA)
First land animal
A millipede
First vascular plants
Cooksonia + agalopython
2 major groups of arthropods and mandibulates originated in ocean
Arthropods: Scorpions, spiders, etc.
Mandibulates: Insects, myriapods, etc.
Appearance of jaws
More diverse diet
Can eat things larger than your mouth
Use of teeth to close the mouth
Devonian (359-419 MA)
Increases weathering & erosion of rocks (Soil)
Allows the rise of forests (358-385 MA)
More photosynthesis -> more oxygen
GHG decreases, temp decreases
Increase in CO2 consumption
Major plant groups evolved except flowering plants
AGE OF FISHES -> enormous increase in biodiversity of fish
Vertebrates move to land "walking fins"
Foot like structure develops
2nd Mass Extinction (Late Devonian 360-375 MA)
Killed 70% of all species
Cause: ocean acidification, volcanism, acid rain
Carboniferous (209-359 MA)
Climate wet & warm
Extensive swamps + rainforests spread around more O2 in atmosphere
Coal reserves formed from remains of those forests
Higher O2 -> creatures could be bigger
Amphibians are dominant vertebrate lifeforms
Age of the Amphibians
Permian (251-298 MA)
Formation of some modern mountain ranges
Sea levels dropped to the lowest point in history.
Temps dropped a lot in temperature
Diversification of 2 of 3 main groups of land vertebrates:
Synapsids
Mammal ancestors
Diapsids
Dinosaur ancestors
3rd Mass Extinction "The Great Dying"
70% of all land organisms
90-95% of all marine species became extinct in 200,000 years.
Causes:
Release of CO2, sulfur dioxide, hydrogen sulfide
Results in global warming, aridity, and ocean acidification
Massive wildfires, deforestation, and soil erosion.
The Mesozoic (66-251 MA)
“Life Rebounding”
Sea temps rose
Fauna: Slow recovery of marine diversity
Flora: Dominated by seeds (no flowers)
First evidence of flowering plants show, but they don’t dominate.
4th Mass Extinction- Triassic/ Jurassic (201.3 MA)
End of triassic mass extinction
Killed 80% of all species
Release of CO2, global warming, and ocean acidification
Jurassic (145-201 MA)
Age of dinosaurs
Saurischia: Pelvis like a lizard
Orinitschia: Pelvis like a bird
Cretaceous (66-145 MA)
Rise of flowering plants
Forests were diverse and dominated by flowering plants
5th Mass Extinction (K/pg- 66 MA)
Killed 75% of all animals, including dinosaurs, invertebrates, and most marine reptiles.
Asteroid hits Earth and Mesozoic ends.
Temp increases a lot
Birds + crocodiles survive.
Cenozoic (66 MA- present)
The age of mammals
Geology and temps warm
Geology and climate
Continents break up
Climate: Slowly cools down temp
Retraction of big forests and expansion of grasslands
Palogene (23-66 MA)
Modern coral reefs
Insects evolve + diversify
Grasses undergo adaptation radiation
Absence of dinosaurs
Proliferation of birds, mammals, and snakes
Mammals didn’t dominate at the beginning of cenozoic
Transition from mammal like reptiles to mammals
Teeth variety
Increased food variety
Acute hearing
Better hearing than reptiles
Locomotion
Move faster, use less energy
Went from sprawling gait -> erect gait
Extinction was the turning point in mammalian evolution
Fast diversification of mammals
In 10 million years, 4000 species of mammals evolved, including aquatic and land mammals.
56 MA: 16 modern placental mammals appeared
35 MA: Modern families appeared
23-34 MA: Climate changes from warm + moist to dry + cool
Great American Biotic Interchange (2.7 MA)
North and South America biota switch places
Pleistocene (2.58 MA- 10 KA)
Homosapiens came to be (300 KA)
The Ice Age
End of the pleistocene
Pleistocene Megafauna Extinction
Humans and climate change caused it
33 out of 45 genera of large mammals became extinct
Anthropocene and 6th mass extinction
Viewed as a period where human activity has had the most dominant influence on climate/ environment.
Biodiversity and Conservation
Learning objectives
1) Define the meaning of biodiversity, distinguish the possible ways of thinking about biodiversity, and criticize the problems of using species as the functional unit of biodiversity measures
2) Explain the concept of hotspots and the factors that regulate diversity
3) Cite and explain the key concepts of diversity and distinguish between them
4) Calculate the Shannon and the Simpson indices and critically evaluate their strengths and limitations
5) Argue about the importance and value of biodiversity
6) Reflect on the main threats to biodiversity and devise solutions to the biodiversity crisis
Adaptation = arose from natural selection
On average there is a direct positive correlation between the size of an area and the number of species it contains. The relationships are shown in a species area curve.
Diversity and area
Habitat diversity
Contains more species in larger areas because they contain more habitats.
Colonization
The number of species in an area may result from a dynamic balance between the number of species colonizing from the source pool (e.g. the mainland set of species for many islands), and those going extinct after colonization
Speciation/ Extinction dynamics
If species with larger geographic ranges have a greater probability of speciating (perhaps because barriers are more likely to subdivide their ranges) and also have a smaller likelihood of extinction (because they contain more individuals, and chance events are less likely to influence them all simultaneously), then more species will accumulate.
Diversity and Energy
Areas where there is a higher incidence of solar radiation should have more energy available to life and, thus, more species.
Biodiversity Hotspots
High number of endemic species (unique to a specific place)
Measuring diversity (key concepts)
Abundance
Total number of organisms in a given area (quantitative)
Richness
Total number of species in a given area (qualitative)
Evenness
A measure of how close in number each species are in a given environment
Diversity
Relationship between number of species and abundance in a certain area
Shannon Diversity Index
Where pi is the proportion of individuals of species (i) in the whole community
N is the number of individuals
Simpson Diversity Index
N= species abundance
n= species evenness
Value of Biodiversity
Direct Use
Food
Medicine
Ecotourism
Recreation
Industrial materials
Tragedy of the Commons
Indirect Use
Pest control
Pollination
Photosynthesis
Climate regulations
Non-use
Preserve for future generations
Future use
Existence is useful to many members in our society
Moral obligation
Threats to biodiversity
Habitat loss
Caused by human settling/ harvesting resources
Overfishing
Usually in fisheries (Tragedy of the Commons)
Invasive Species
Outcompete and feed on others
Brings disease to endemic species
Climate Change
Rapid global temp change which makes zones uninhabitable to endemic species
What to do?
Gather data
Study biodiversity
Change human behavior
Legislation, international conventions
Preserve pristine areas
National Parks, etc.
Habitat restoration
Reconstruct lost biodiversity
Preserve genetic diversity
Experimental Design
Learning Objectives
1) Identify the main steps of the process of designing an experiment
2) Explain the terms involved in an experiment such as: control, variables, etc.
3) Based on a question, propose a scientific hypothesis
4) Design an experiment that can test a hypothesis, obeying the principles of scientific methodology (falsifiability and repeatability)
Observation (data/facts)
What you can see/smell/taste/hear/measure
Inference (Hypotheses/ educated guesses)
Conclusions you can make given past knowledge/ context
Mendel
Observed
Different plants could be crossed and their offspring could have features of ancestors.
Inference
How do plants inherit features of their parents?
Experimental design
Create an effective scientific experiment to answer a scientific question
Think about these…
Can my question be answered by my experiment?
Did someone answer this before?
Is the experiment feasible?
What are my variables?
How will my results confirm or refute my hypothesis?
Good characteristics of peas to experiment
Variety
Convenience
Numbers
Easy to cross
Planning the experiment
Need variables (things that can influence results)
Independent variables
Changed by scientists
Dependent variables
Responds to independent variable
Controlled variables
Try to maintain and isolate them to stay the same
Conditions (methodology and procedure)
Different number of treatments you’ll do
Control
Treatment that you know beforehand the results
Positive control
Validates procedure
Negative control
Negative outcome
Collect data and analyze results
Mendel concluded…
Hereditary traits are controlled by factors that exist in pairs
Factor spread randomly throughout the gametes
Some traits are stronger than others