Forensic Evidence Analysis Summary
Evidence Handling and Analysis
- Liquid Dilution: Conduct liquid dilution if liquid evidence is present; ensure positive reports include references to the product tested.
- Soil Evidence: Soil samples must be refrigerated or frozen to prevent microbial degradation.
Fingerprint Analysis
- Comparative Processes: ACEV (Analysis, Comparison, Evaluation, Verification) is used for fingerprint comparison. Known prints are essential for matching suspects.
- Reverse Searches: Unsolved prints are run through reverse searches to identify potential matches.
- AFIS: The Automated Fingerprint Identification System (AFIS) uses state identifiers for tracking.
DNA Forensics
- Sample Collection: Ensure cleanliness by changing gloves to avoid cross-contamination when handling DNA. Samples are placed in labeled test tubes, marked with specific identifiers.
- Chemical Reagents: DTT and protein kinase are used for breaking down cells and deactivating harmful enzymes. The extraction process employs HTL buffer to isolate DNA from cells.
Print Development Techniques
- Super Glue Fuming: Utilized for developing latent prints on non-porous surfaces. This involves submerging objects in a chamber to capture fingerprints effectively.
- Ninhydrin Application: For developing prints on paper surfaces; typically set for 24 hours and then heated in an oven at 100°C.
Quality Control in Forensics
- Sample Processing: Samples must be centrifuged to separate components and ensure clarity. Different machines should be used for evidence and reference samples to reduce error rates.
- Quantitative Analysis: A thermomixer is used for controlling the temperature during processing, and quality control is essential after running the samples to ensure reliability in results.