Exam Study Notes

Skeletal Muscle

  • Deals with voluntary movement; has striations (striped appearance). Muscle cells are very long cells called fibers.
  • Multinucleate: Has many nuclei around the membrane.
  • Sarcolemma: The membrane surrounding the muscle fiber.
  • Musculoskeletal system: Composed of muscle fibers with connective tissue wrappings.
  • Epimysium: Dense fibrous connective tissue surrounding the entire muscle.
  • Muscle fibers are the cells themselves; when discussing nerves, the term "fiber" refers to axons.
  • Skeletal muscle cells are very long and therefore called fibers.
  • Connective tissue is continuous with the tendons and periosteum of the bone.
  • Tendons: Connect muscle to bone.
  • Ligaments: Connect bone to bone.
  • Isometric contraction: Contraction without movement.
  • Paramecium: Thin projections of connective tissue that extend from the epimysium into the muscle to surround bundles of muscle fibers known as fascicles.
  • Endomycium: Loose connective tissue within each fascicle that separates individual muscle fibers.
  • Muscle Fiber Structure
    • Single, elongated cell surrounded by a plasma membrane (sarcolemma).
    • Multiple nuclei located just beneath the sarcolemma.
    • Myofibrils: Thread-like structures that extend the length of the fiber and dominate its interior.
    • Myofilaments: Protein filaments within myofibrils.
      • Actin: Thin myofilaments.
      • Myosin: Thick myofilaments.
  • Sarcomere
    • Contractile unit of the muscle.
    • Formed by the orderly arrangement of actin and myosin myofilaments.
    • Sliding of filaments shortens the sarcomere, leading to muscle contraction.
  • Muscle Contraction
    • In a relaxed muscle, actin and myosin lie side by side; H zones and I band are at maximum width.
    • During contraction, actin slides toward the center of each myosin myofilament, shortening the sarcomere.
    • In a fully contracted muscle, actin myofilaments overlap, H zones disappear, and the I band becomes very narrow.
  • Mechanism of Muscle Contraction
    • Action potential is introduced at the neuromuscular junction and propagates along the sarcolemma.
    • Action potential propagates along the T-tubule membrane into the interior of the muscle cell near the sarcoplasmic reticulum.
    • Depolarization of the T-tubule membrane causes voltage-gated calcium ion channels on the sarcoplasmic reticulum to open.
    • Calcium ions diffuse from the sarcoplasmic reticulum into the sarcoplasm.
    • Tropomyosin proteins cover myosin binding sites on actin myofilaments.
    • Troponin proteins are attached to tropomyosin.
    • Calcium ions bind to troponin, causing it to change conformation and move tropomyosin.
    • Uncovering myosin binding sites on actin allows myosin heads to bind and form cross-bridges.
    • Cross-bridges play a role in muscle contraction.

Meiosis

  • Myopia cells reproduce by mitosis, producing two identical daughter cells, except in the reproductive system.
  • Meiosis: An extra step is needed to reduce the chromosome number to haploid.
  • Diploid number: 46 chromosomes in a normal human cell.
  • Haploid number: 23 chromosomes in reproductive cells, allowing each parent to contribute half the genetic code.
  • Meiosis results in the formation of sperm cells and egg cells.
  • Germline cells: Diploid cells in the testes and ovaries that undergo meiosis.
  • Meiosis produces haploid cells with one set of chromosomes.
  • Fertilization: Haploid cells fuse to form a diploid offspring.
  • Meiosis is preceded by interphase, during which DNA replicates, and each chromosome becomes doubled.
  • Meiosis involves two divisions:
    • Meiosis I: Halves the number of chromosomes.
    • Meiosis II: Splits the sister chromatids.
  • End result: Four haploid cells.
  • Meiosis I
    • Prophase I: Individual replicated chromosomes become visible; each chromosome consists of identical sister chromatids joined at their centromeres. Spindle fibers start to form the nuclear envelope disintegrates. Homologous chromosomes line up next to each other.
    • Crossing over: Homologous chromosomes exchange DNA between adjacent homologous chromatids.
    • Metaphase I: Homologous chromosomes line up along the equator of the cell. The orientation on the equator is random (independent assortment).
    • Anaphase I: Chromosomes move apart from one another along the spindle fiber to the opposite ends of the cell. Each chromosome is still double-stranded.
    • Telophase I: The spindle fiber disintegrates, and cytokinesis begins. Two cells are created, each with half the number of chromosomes compared to the original cell.
  • Meiosis II
    • Prophase II: Spindle fibers form at the poles of the cell.
    • Metaphase II: Chromosomes line up along the equator (different from metaphase I, where homologous chromosomes were lined up).
    • Anaphase II: Sister chromatids move away from each other along the spindle fiber.
    • Telophase II: Four genetically different haploid cells are formed, along with cytokinesis.

Female Reproductive Cycle

  • Initiation: Begins at puberty, usually between the ages of 10 and 14.
  • Cycle Length: Averages 28 days.
  • Hormonal Control: Gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates the anterior pituitary to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
  • Target Organ: Ovary.
  • Two Components:
    • Ovarian cycle
    • Uterine cycle
  • Ovarian Cycle
    • Follicular phase: The 14 days prior to ovulation.
    • Ovulation: Occurs on day 14 of a 28-day cycle.
    • Luteal phase: The 14 days following ovulation.
  • Uterine Cycle
    • Menstrual phase: Days 1-5 (sloughing of the endometrial lining).
    • Proliferative phase: Days 6-14 (endometrium begins to reform).
    • Secretory phase: The 14 days after ovulation (further thickening of the endometrium).
  • Follicular Phase Events
    • Increased GnRH stimulates the anterior pituitary to increase production of FSH and LH.
    • Primordial follicles in the ovary mature into primary follicles, each containing a diploid primary oocyte arrested in prophase I of meiosis I.
    • Decreasing levels of progesterone lead to sloughing of the endometrial lining (menstrual phase).
    • Primary follicles form secondary follicles.
    • About 16 hours before ovulation, FSH and LH surge, causing final maturation of the follicle (mature or Grafian follicle).
    • Primary oocyte completes meiosis I to form a haploid secondary oocyte.
    • The endometrium begins to reform under the influence of estrogen produced by the ovarian follicular cells (proliferative phase).
  • Ovulation
    • The level of LH peaks, causing proteolytic enzymes to weaken and rupture the follicle wall.
    • Release of the secondary oocyte from the mature follicle.
  • Luteal Phase Events
    • Remaining ovarian follicular cells form a corpus luteum, which produces progesterone and estrogen.
    • Progesterone and estrogen stimulate further thickening of the endometrium (secretory phase).
  • Fertilization and Implantation
    • Cells at the implantation site produce human chorionic gonadotropin (HCG).
    • HCG prevents degeneration of the corpus luteum, maintaining elevated progesterone and estrogen levels.
    • After 8-12 weeks, the placenta replaces the corpus luteum in producing progesterone and estrogen.
  • No Fertilization
    • The corpus luteum becomes a corpus albicans.
    • Decreased progesterone and estrogen levels lead to menstruation.

Vision

  • Structures involved: Eye, optic nerve, and portions of the brain.
  • Cornea: Transparent connective tissue that covers the anterior one-sixth of the eye; refracts light.
  • Pupil: Opening in the colored portion of the eye.
  • Lens: Biconvex lens that focuses light onto the retina.
  • Retina: Lines the interior surface of the eye.
    • Retinal pigment epithelium: Prevents light scatter within the eye.
    • Neural retina: Multiple cell types arranged in three cell layers.
      • Outer layer:
        • Rod photoreceptor cells: Used in poor lighting conditions, low spatial resolution, no color vision.
        • Cone photoreceptor cells : Used for high spatial resolution and color vision.
      • Inner nuclear layer: Processes signals from rods and cones.
      • Ganglionic layer: Transmits signals to the optic nerve.
  • Optic Nerve
    • Formed by axons of retinal ganglion cells.
    • Attaches to the ventral aspect of the brain at the optic chiasm.
  • Optic Tracts
    • Extend around the brainstem to the lateral geniculate nucleus of the thalamus.
  • Lateral Geniculate Nucleus
    • Most ganglion cell axons from the retina end at synapses here.
  • Optic Radiations
    • Visual information leaves the thalamus in the optic radiations.
  • Primary Visual Cortex
    • Located in the cerebral occipital lobe.
    • Where visual information is perceived as an image.

Hearing

  • Sound waves strike the tympanic membrane (eardrum), causing it to vibrate.
  • Tympanic Membrane: Separates the outer ear from the middle ear.
  • Auditory Ossicles (Malleus, Incus, Stapes)
    • Three bones of the middle ear that vibrate.
    • The footplate of the stapes vibrates in the oval window.
  • Vibration of the footplate causes perilymph in the scala vestibuli to vibrate, displacing the basilar membrane.
  • Basilar Membrane
    • Short wavelengths (high-pitched sounds) cause displacement near the oval window.
    • Long wavelengths (low-pitched sounds) cause displacement far from the oval window.
    • Movement is detected by hair cells of the spiral organ.
  • Vibrations reach the perilymph in the scala tympani and travel to the round window, where they are dampened.

Meninges

  • Three membranes that protect the brain and spinal cord.
  • Meningitis: Inflammation of the meninges.
  • Layers (superficial to deep):
    • Dura mater
    • Arachnoid mater
    • Pia mater
  • Dura Mater (Cranial Cavity)
    • Two sublayers:
      • Periosteal layer: Tightly adherent to the inner surface of the skull.
      • Meningeal layer: Inner portion of the dura.
    • Dural venous sinuses: Formed where the periosteal and meningeal layers split; collect venous blood from the brain.
  • Arachnoid Mater
    • Thin transparent membrane in direct contact with the overlying dura.
    • Includes web-like extensions to the pia mater.
  • Subarachnoid Space
    • Between the arachnoid and pia mater.
    • Filled with cerebrospinal fluid.
  • Pia Mater
    • Delicate layer of connective tissue.
    • Located directly on the surface of the brain and spinal cord.
    • Follows all surface contours.