FS 101: Test 3

SEPTEMBER 30th - Fermentation

  • History

    • 9000 BCE

      • rice fermentation in China

    • 5000 BCE

      • beer and wine production in egypt and mesopotamia

    • 500 BCE

      • vegetable fermentation in China

  • Science

    • metabolic pathways

      • aerobic vs. anaerobic

        • need an anaerobic environment for fermentation

        • preserves food by creating environment unsuitable for spoilage and for maintaining microbes

      • glycolysis

        • initial breakdown of glucose into pyruvate, followed by fermentation equaling ATP and by products

    • stages

      • lag phase: introduction of fermentation conditions

      • initial growth phase: rapid growth and consumption

      • stationary/stabilization phase: by-products accumulate, inhibiting any further growth

      • decline phase: deterioration overtime

    • key microorganisms

      • lactic acid bacteria

        • converts sugars into lactic acid

        • benefits

          • extends shelf life by lowing pH

          • development of tangly flavors and aromas

      • yeasts

        • converts sugars into alcohol and CO2

        • benefits

          • alcohol is a natural preservative

          • develops unique flavor

      • molds

        • production of soy products and certain cheeses

        • benefits

          • develops savory umami flavors and buttery notes

  • benefits

    • textural

      • creaminess (yogurt)

      • crispness (pickles)

      • aeration (bread)

    • nutritional

      • breaks down antinutrients

      • increases avaliability of nutritional factors

      • probiotic contents (lactobacillus and bifidobacterium)

      • gut health support

      • microbiome diversity

  • potential risks

    • excessive salt intake

    • contamination risks

  • products

    • dairy

      • yogurt

        • pasteurized milk + LAB cultures + fermentation at 42-45 C → coagulation of proteins → cooling and packaging

      • cheese

        • pasteurized milk + rennet + LAB → curdling → draining of whey → aging

        • flavors

          • aging = flavors, texture, and nutritional properties

          • many varieties = milk type, fermentation time and cultures

    • vegetables

      • sauerkraut

        • cabbage + salt → anaerobic conditions → LAB sugar to Lactic Acid

        • benefits

          • shelf life, gut health, high in vitamin C

      • kimchi

        • cabbage + radish + garlic + spices + salt → LAB sugar to Lactic Acid

        • benefits

          • spicy, tangy flavor

          • nutritional benefits

    • Grains

      • bread

        • yeast ferments sugars in dough → CO2 → rising

        • characteristics

          • light airy texture

          • enhanced flavor

          • aromatic quality: alcohol and organic acids = bread flavor

        • sourdough

          • wild yeast and LAB, long fermentation time leading to tangy flavor

        • commercial

          • bakers yeast and LAB, short fermentation time leading to less complex flavor

    • beer

      • malted grains + yeasts + fermentation → conversion sugar to alcohol + CO2 → filter and processing

      • alcohol content: time of fermentation

      • flavor

        • ingredients change flavor

          • hops, citrus, etc.


OCTOBER 4th - Food deterioration

  • food deterioration: the process in which food undergoes changes that make it unsuitable for concumption

    • causes

      • microbial activity

      • enzymatic reactions: natural enzymes in food, cause spoilage ex: brown apples

      • chemical reactions: oxidation and hydrolysis

      • physical changes: moisture loss, texture degradation

      • environmental factors: temperature, humidity, light exposure

    • food borne microorganisms

      • bacteria

        • salmonella, E.Coli, etc.

        • grow rapidly under favorable conditions

        • undercooked meat, unpasteuized dairy, contaminated vegetables

OCTOBER 9th - Food borne Illnesses

  • Food borne illnesses

    • types

      • salmonella

        • raw poultry, eggs, dairy, contaminated vegetables

        • causes diarrhea, fever, gastroenteritis

        • prevented by proper hygiene among animals, raw meat, and washing vegetables

      • E.coli (Escherichia coli)

        • undercooked beef, unpasteurized milk

        • causes stomach cramps, bloody diarrhea, vomiting

      • Listeria monocytogenes

        • ready-to-eat meat, unpasteurized dairy, smoked seafood

        • causes diarrhea, nausea, etc.

      • norovirus

        • water, shellfish

        • nausea, diarrhea, stomach pain

    • prevention

      • proper cooking

      • temperature control

        • cold below 40 F

        • hot above 140 F

      • avoid cross contamination

        • raw meats away from veggies, etc.

      • safe storage

        • temperature, immediate after process

      • use of preservatives

        • sorbates, vinegars, etc.

    • outbreaks

      • find sources

      • trace to common point of contamination

      • confirm with lab testing

  • Food borne microorganism

    • molds

      • grown on food surfaces, visible colonies

        • apsergillus, penicillium

          • some have mycotoxins harmful when ingested.

            • some beneficial ex cheese

      • viruses

        • norovirus, hepatitius A

        • cannot grow on food but are transmitted through contaminated food or water

        • require living host to reproduce and operate

  • factors effecting microbial growth

    • intrinsic:

      • water activity

      • pH

      • nutrient content

      • redox potential: oxygen availability

    • extrinsic

      • temperature

      • humidity

      • gaseous environment


OCTOBER 11th - Dehydration

  • definition

    • removing moisture from food to extend shelf life

  • importance

    • reduces AW, prevents spoilage, storage efficiency, preserved food quality

  • applications

    • fruits

    • vegetables

    • coffee

    • dairy

    • meat

  • scientific principles

    • evaporation: removal of moisture from food surface

    • diffusion: moisture migrates from inside to the surface

    • equilibrium moisture constant: point where food no longer looses moisture in drying process

    • mass transfer: inside → surface → air

    • heat transfer: process of exchanging thermal energy between different systems or objects due to change in temperature

      • conduction: heat through surface, without moving material itself

      • convection: heat through gas/liquid, through circulation “convection currents”

      • radiation: heat through electromagnetic waves, doesn’t require medium

  • challenges

    • uniformity

    • energy consumption

    • quality retention, different flavor, color, texture

    • loss of nutrients

    • varying rehydration quality

  • methods

    • sun drying

      • oldest

      • low cost

      • unreliable, potentially unclean

    • air drying

      • faster

      • loss of heat sensitive compounds

    • tray drying

      • industrial, small scale

      • batch method

      • easier to control airflow

      • leads to case hardening

    • spray drying

      • liquid sprayed into chamber with hot air

      • rapid, good for heat-sensitive products

      • energy consumption, expensive

    • freeze drying

      • sublimation via vacuum of frozen products

      • expensive

    • drum drying

      • slurry or puree applied as thin layer onto revolving drums

    • fluidized bed drying

      • food suspended and dried by hot air

    • microwave drying

      • uses electromagnetic waves to dry

      • fast, and retains nutrients

    • infrared drying

      • fast, uniform

      • causes case hardening

OCTOBER 14th - Thermal Processing

  • definition: using heat to treat or prepare food, beverages, or other substances

  • importance

    • extension shelf life

    • destruction of microorganisms

    • improved safety

    • improved quality

    • convenience

  • history

    • 19th mid - pasteurization

    • late 19th - microbiology + technology increases, and commercial canning industry increases

    • mid 20th - sterilization and pressure cooking

    • late 20th - microwave and sous vide

    • early 21st - continued sustainability advancements

  • principles

    • higher temperature → shorter processing time

      • want temperature and time balance that targets specific microbe at coldest part of product

  • factors

    • temperature = higher → microbe destruction

    • time = processing time at time → efficacy

    • pH = extreme pH inhibits growth

    • moisture = high AW → increased growth

    • food composition = effects heat penetration and distribution

    • microbial characteristics = varying microorganisms have heat resistance

    • packaging = vacuum or modified atmospheric packaging effects microorganism

    • agitation = stirring, uniformity

    • equipment = uniformity, safety

  • basic numbers

    • d-value = time to decrease population of microbes by 90% at a certain temperature

      • measures sensitivity to heat

    • z-value = temperature change needed to alter d by factor of 10

      • measures sensitivity to change in temperature

    • f-value = cumulative lethality

      • equivalent time at a reference temperature that achieves same microbial destruction as actual process progresses at a variable temperature

  • pasteurization

    • mild heat treatment to kill pathogens without affecting food quality

    • milk, juice, eggs, etc.

    • HTST: high temp, short time

    • LTLT: low temp, long time

    • targeted microbes

      • coxiella burnetii

      • salmonella enteritidis

      • e.coli

  • sterilization

    • severe heat treatment to kill all life forms

    • commercial sterilization: free of microbes able to reproduce under typical storage

    • methods

      • in container sterilization: sealed and heated to achieve commercial sterility

      • UHT: heating at +135 C before packaging

  • processes

    • retorts

    • pressure cookers

    • aseptic processing

    • blanching: short heat treatment used to inactivate enzymes and prepare food for further processing

    • dehydration

    • baking/cooking

    • frying: submerging foods in hot oil, quick to preserve

  • effects on food

    • texture

      • softening → break down cell walls

      • gelling

    • nutrient retention

      • can degrade nutrition

    • color

      • browning → when controlled, can enhance appearance

      • fading → pigment degradation in fruit and vegetables

  • challenges

    • overprocessing

      • excessive heat → quality, nutrition, flavor degradation

    • under processing

      • insufficient heat → food safety risks

    • heat distribution

      • uneven heat distribution can cause over and under processing

      • equipment design + probes can regulate and fix

    • energy consumption

      • sustainability, costs, etc.