WEEK 6.1 GIE Reproduction Study Notes

Endocrinology of Reproduction Study Notes

Physiological Control of Reproduction

  • Instructor: Dr. Alex Cheong

Subjects Covered

  • Anatomy

  • Male Reproductive Physiology

  • Spermatogenesis

  • Hormonal Control

  • Female Reproductive Physiology

  • Oogenesis

  • Menstrual Cycle

  • Hormonal Control

Male Reproductive System Anatomy

  • Components:

    • Urinary bladder

    • Ureter

    • Bulbourethral gland

    • Ductus deferens

    • Bulb

    • Epididymis

    • Testis

    • Urethra

    • Glans of penis

    • Seminal vesicle

    • Prostate gland

Key Points About Male Reproductive Anatomy

  • Testes:

    • Contain Sertoli and Leydig cells for testosterone production.

    • Composed of lobules with sperm-producing tubes.

  • Spermatogonium:

    • Cells responsible for sperm production.

  • Epididymis and Ductus Deferens:

    • Sites for maturation, storage, and exit of sperm.

  • Prostate Gland:

    • Secretes a thin milky white seminal fluid to alkalinize the vagina and enhance sperm motility.

  • Seminal Vesicles:

    • Store seminal fluid rich in fructose, prostaglandins, and clotting factors.

Detailed Anatomy of the Testes

  • Components:

    • Vas deferens: transports sperm.

    • Efferent ductules: connect seminiferous tubules to epididymis.

    • Rete testis: Network of tubules that transport sperm.

    • Seminiferous tubules: Site of spermatogenesis.

  • Leydig Cells: Interstitial cells that secrete testosterone.

  • Sertoli Cells: Support and nourish developing sperm and form the blood-testis barrier through tight junctions.

Functions of Sertoli Cells

  • Form the blood-testis barrier.

  • Provide nourishment to developing sperm.

  • Phagocytose dead spermatogenic cells.

  • Secrete fluid that flushes sperm into the epididymis.

  • Produce androgen-binding protein (ABP) to maintain high testosterone levels in the testes.

  • Act in response to follicle-stimulating hormone (FSH) and testosterone.

Leydig Cells Overview

  • Site: Located within connective tissue between seminiferous tubules.

  • Function: Distinct from spermatogenesis; they secrete testosterone.

  • Hormonal Control: Stimulated by luteinizing hormone (LH).

  • Testosterone:

    • A steroid hormone derived from cholesterol.

    • Secreted into the blood, often bound to plasma proteins, including ABP from Sertoli cells.

Testosterone Actions

  • Refer to textbooks for detailed physiological actions of testosterone.

Hormonal Control of Testicular Function

  • Diagram showing control by the hypothalamus and pituitary, including the role of Gonadotrophin-releasing hormone (GnRH) from the hypothalamus:

    • Activates the pituitary to produce LH and FSH.

    • Sertoli Cells respond to these hormones to stimulate sperm production.

Spermatogenesis

  • Duration: Continuous process, with a single sperm taking approximately 64 days to mature.

  • Volume: 200 million sperm present at any one time.

Spermatogenesis Process
  • Initial Stage: Spermatogonium undergoes mitosis.

  • Primary Spermatocyte undergoes meiosis I to form two secondary spermatocytes.

  • Secondary spermatocytes undergo meiosis II to yield four spermatids.

Summary of Meiosis and Mitosis
  • Meiosis: 1n results in gametes, producing genetic variability through two rounds of division.

  • Mitosis: 2n leading to cloning of cells for growth and repair.

Prostate Gland Anatomy

  • Location: Positioned near the bladder and surrounding the sperm duct.

  • Function: Important in secretion of seminal fluid for sperm viability.

Male Fertility Factors

  • Seminal fluid improves sperm viability by alkalinizing the acidic environment of the vagina.

  • Challenges for sperm: Must travel through cervical mucus and face immune cells that may attack sperm.

  • Average sperm swim speed is approximately 5 mm/min, attributed in part to muscular movements in the female reproductive tract.

Female Reproductive Anatomy

  • Components:

    • Ovary (active endocrine organ)

    • Fallopian tubes

    • Uterus

    • Cervix

    • Vagina

Female Reproductive Cycle

  • Follicular Phase: Developing follicle secretes estrogen.

  • Luteal Phase: Corpus luteum secretes progesterone, essential for maintaining uterine lining.

Menstrual Cycle Phases
  • Hypothalamus releases GnRH, stimulating the anterior pituitary for FSH/LH release.

  • FSH stimulates follicle growth; LH surge triggers ovulation.

  • Follicular phase increases estrogen levels, thickening the endometrial lining.

  • Post-ovulation: Corpus luteum secretes hormones to prepare for potential implantation.

Folliculogenesis Control

  • Initiation by FSH, which stimulates estrogen production from developing follicles.

  • Positive feedback loop enhances more FSH secretion.

  • Mid-cycle LH surge triggers ovulation and formation of the corpus luteum.

Post-Ovulation Hormonal Changes
  • Corpus luteum secretes progesterone and estrogen, along with inhibin to regulate FSH and LH secretion.

  • Former corpus luteum, if not successful in implantation, degenerates into corpus albicans.

Charts and Hormonal Levels

  • Hormone Levels and phases related to follicular and luteal cycles shown in graphical format.

    • Basal body temperature changes throughout menstrual cycle phases as influenced by hormone levels.

Fertilization and Pregnancy Process

  • Fertilization: Sperm and egg nuclei fusion creates zygote, followed by cell division.

  • Implantation: Blastocyst adheres to endometrial lining; trophoblast secretes enzymes to facilitate embedding into endometrium for nutrient access.

  • HCG: Human chorionic gonadotropin is secreted after implantation, preserving the corpus luteum and hormonal production.

Not Pregnant Scenario

  • Absence of implantation results in corpus luteum degeneration, decreased progesterone levels, and resets hormonal regulation for a new cycle.

Comparison of Female and Male Reproduction

  • Females release about 400 eggs over their lifetime, whereas males produce around 200 million sperm continuously.

  • Female reproductive activity is cyclical, while male spermatogenesis is a continuous process.

Contraception and Manipulation of Reproductive Physiology

  • Understanding reproductive physiology aids in the development of reliable contraceptive methods.

Future Lectures

  • Introduction to contraception and infertility.

  • Discussion on menopause.

  • Overview of common gynecological disorders.