Detailed Lab Notes on Terrestrialization and Plant Biology

Pre-Lab Preparation

  • Read Lab Introduction: Familiarize yourself with concepts before lab.
  • Complete Pre-Lab Assignments: Watch videos and assignments thoroughly.
  • Review Key Terminology: Understand important terms relevant to the lab (see provided list).
  • Organismal Biology Lectures: Review lectures for foundational knowledge.
  • Study Mitosis and Meiosis: Focus on the ploidy of initial and resulting cells; detailed phases not required.

Lab Materials

  • To Bring:
    • Lab introduction and terminology list (digital access is acceptable).
    • Textbook or digital e-text access (check bCourses for instructions).

Cyanobacteria

  • Definition: Microscopic organisms in aquatic and terrestrial ecosystems, known for the pigment phycocyanin used in photosynthesis.
  • Ecological Role: Major contributors to primary production, essential for carbon capture on Earth; part of marine and freshwater phytoplankton.
  • Cellular Structure:
    • Prokaryotic: Lack membrane-bound organelles (nucleus, mitochondria, chloroplasts).
    • Cell Wall: Prokaryotic walls differ structurally from eukaryotic cell walls.
  • Reproduction: Asexual through binary fission; do not reproduce sexually.

Photoautotrophy

  • Definition: Organisms that produce their own food using sunlight. Cyanobacteria use CO₂ and light to synthesize glucose, releasing oxygen as a by-product.
  • Historical Significance: Contributed to the Great Oxygenation Event over 2 billion years ago.

Endosymbiosis

  • Concept: The theory that chloroplasts in green plants originated from symbiotic cyanobacteria incorporated into eukaryotic cells.

Cellular Organization of Cyanobacteria

  • Colonial Forms: Can exist as unicellular or aggregate into filaments, sheets, or hollow spheres.
  • Specialized Cells: Some filaments contain heterocysts for nitrogen fixation, showcasing functional differentiation within colonies.

Dietary Use of Spirulina

  • Spirulina: High in protein, used as a supplement; historically significant for indigenous cultures (e.g., Mexicas and Kanembu people).

Cyanobacterial Blooms

  • Causes: Fluctuations in light, temperature, pH, nutrients (N and P) can lead to harmful blooms, producing cyanotoxins.
  • Eutrophication: Nutrient overload can lead to decreased oxygen, harming aquatic life.
  • Human Impact: Increased blooms due to anthropogenic nutrient discharges (wastewater, livestock, agriculture).

Volvox Overview

  • Definition: Colonial freshwater green algae, belonging to eukaryotes; contains chlorophyll, hence green color.
  • Structure: Hollow spheres of cells in a gelatinous matrix with cytoplasmic connections for organelle sharing and coordination of movement.
  • Cell Types: Composed of somatic cells (movement, photosynthesis) and germ cells (reproduction).
  • Reproduction: Can reproduce asexually (daughter colonies) or sexually (in response to environmental stress).

Multicellular Green Algae: Ulva

  • Common Name: Sea lettuce; grows in coastal environments.
  • Structure: Composed of two layers of cells; forms a holdfast for anchoring.
  • Life Cycle: Haplodiplontic; alternates between multicellular diploid sporophyte and haploid gametophyte, with isomorphic conditions (similar morphology of both generations).

Chara Characteristics

  • Relation to Land Plants: Closest relatives of land plants, sharing traits like zygote retention and similar flagella structure.
  • Reproductive Structures: Female gametes are produced in oogonia, and male in antheridia; the zygote undergoes meiosis to produce spores directly.