Detailed Lab Notes on Terrestrialization and Plant Biology
Pre-Lab Preparation
- Read Lab Introduction: Familiarize yourself with concepts before lab.
- Complete Pre-Lab Assignments: Watch videos and assignments thoroughly.
- Review Key Terminology: Understand important terms relevant to the lab (see provided list).
- Organismal Biology Lectures: Review lectures for foundational knowledge.
- Study Mitosis and Meiosis: Focus on the ploidy of initial and resulting cells; detailed phases not required.
Lab Materials
- To Bring:
- Lab introduction and terminology list (digital access is acceptable).
- Textbook or digital e-text access (check bCourses for instructions).
Cyanobacteria
- Definition: Microscopic organisms in aquatic and terrestrial ecosystems, known for the pigment phycocyanin used in photosynthesis.
- Ecological Role: Major contributors to primary production, essential for carbon capture on Earth; part of marine and freshwater phytoplankton.
- Cellular Structure:
- Prokaryotic: Lack membrane-bound organelles (nucleus, mitochondria, chloroplasts).
- Cell Wall: Prokaryotic walls differ structurally from eukaryotic cell walls.
- Reproduction: Asexual through binary fission; do not reproduce sexually.
Photoautotrophy
- Definition: Organisms that produce their own food using sunlight. Cyanobacteria use CO₂ and light to synthesize glucose, releasing oxygen as a by-product.
- Historical Significance: Contributed to the Great Oxygenation Event over 2 billion years ago.
Endosymbiosis
- Concept: The theory that chloroplasts in green plants originated from symbiotic cyanobacteria incorporated into eukaryotic cells.
Cellular Organization of Cyanobacteria
- Colonial Forms: Can exist as unicellular or aggregate into filaments, sheets, or hollow spheres.
- Specialized Cells: Some filaments contain heterocysts for nitrogen fixation, showcasing functional differentiation within colonies.
Dietary Use of Spirulina
- Spirulina: High in protein, used as a supplement; historically significant for indigenous cultures (e.g., Mexicas and Kanembu people).
Cyanobacterial Blooms
- Causes: Fluctuations in light, temperature, pH, nutrients (N and P) can lead to harmful blooms, producing cyanotoxins.
- Eutrophication: Nutrient overload can lead to decreased oxygen, harming aquatic life.
- Human Impact: Increased blooms due to anthropogenic nutrient discharges (wastewater, livestock, agriculture).
Volvox Overview
- Definition: Colonial freshwater green algae, belonging to eukaryotes; contains chlorophyll, hence green color.
- Structure: Hollow spheres of cells in a gelatinous matrix with cytoplasmic connections for organelle sharing and coordination of movement.
- Cell Types: Composed of somatic cells (movement, photosynthesis) and germ cells (reproduction).
- Reproduction: Can reproduce asexually (daughter colonies) or sexually (in response to environmental stress).
Multicellular Green Algae: Ulva
- Common Name: Sea lettuce; grows in coastal environments.
- Structure: Composed of two layers of cells; forms a holdfast for anchoring.
- Life Cycle: Haplodiplontic; alternates between multicellular diploid sporophyte and haploid gametophyte, with isomorphic conditions (similar morphology of both generations).
Chara Characteristics
- Relation to Land Plants: Closest relatives of land plants, sharing traits like zygote retention and similar flagella structure.
- Reproductive Structures: Female gametes are produced in oogonia, and male in antheridia; the zygote undergoes meiosis to produce spores directly.