chapter 24

Hormones

  • Definition: Chemical signals produced by cells in certain organs and tissues.
  • Mechanism: Hormones are released into the bloodstream and transmitted throughout the body.
  • Function: They bind to specific receptors on the surface of various cells, which triggers changes in gene expression.

Gene Expression Control

  • Processes involved in regulating gene expression:
    • Transcription: The process by which mRNA is synthesized from DNA.
    • Translation: The conversion of mRNA information into polypeptides.
    • Post-translational modification: The processes that modify polypeptides after translation, including:
    • Trimming (removing certain parts).
    • Addition of functional groups.
    • Protein folding (to form functional proteins).

mRNA Features

  • mRNA modifications:
    • Addition of a 5’ cap and 3’ poly-A tail, aiding in nuclear export and translation.
    • Splicing: Removal of non-coding sequences (introns) from mRNA before translation.

Ribosomes and Protein Synthesis

  • Ribosomes: The organelles responsible for synthesizing polypeptides.
  • Rough Endoplasmic Reticulum (ER): Ribosomes attached to the rough ER assist in synthesizing secretory proteins.
  • Golgi Apparatus: Processes, modifies, and packages proteins synthesized in the rough ER.

Protein Modification and Immune System

  • Post-translational modifications occur in:
    • Rough ER
    • Golgi apparatus
  • Importance in immune response: Immunological proteins, such as antibodies, are synthesized and modified in these organelles.

Bone Marrow Stem Cells and Differentiation

  • Bone marrow stem cells can differentiate into various blood cell types:
    • Signals received by these cells determine gene activation and cell differentiation.
    • Example: Some stem cells receive signals to differentiate into B cells, while others become T cells.

Cancer and Gene Expression

  • Overview: Cancer is characterized by uncontrolled cell division resulting from mutations affecting oncogenes and tumor suppressor genes.
  • Oncogenes: Mutated proto-oncogenes that promote cell division when they should not.
  • Tumor suppressor genes: Normal genes that limit cell growth and division; loss of function due to mutations can lead to cancer.
  • Example: BRCA1 and BRCA2 genes associated with breast and ovarian cancer.

Role of Signals in Gene Expression

  • The activation of oncogenes and the inactivation of tumor suppressors contribute to cancer progression.
  • Growth factors stimulate cells through receptor engagement, impacting mitosis regulation.

Immune System Overview

  • Two major subdivisions:
    • Innate Immune System: Rapid, non-specific response
    • Involves immediate responses such as inflammation and phagocytosis by macrophages.
    • Adaptive Immune System: Slower, specific response that involves B and T cells.
    • Generates immunological memory following an infection.

Inflammatory Response

  • Mechanism: Initiated by macrophages or mast cells detecting pathogens.
  • Steps involved:
    1. Release of signals to call for immune support.
    2. Increased blood flow to the area, allowing more immune cells to arrive.
    3. Phagocytosis: Immune cells like macrophages engulf pathogens and debris.

T Cells and B Cells

  • T Cells:
    • Helper T Cells: Activate other immune cells.
    • Cytotoxic T Cells: Destroy infected or cancerous cells.
  • B Cells:
    • Develop into plasma cells that secrete antibodies against specific antigens.
    • Generate memory cells for long-term immunity.

Antigen Recognition

  • Antigens: Molecules that trigger an immune response (e.g., components of pathogens).
    • Specific to pathogen surface proteins, enabling immune targeting.

Vaccination Mechanisms

  • Vaccines work by simulating an infection, training the immune system without causing disease.
  • Types of vaccines:
    1. Live attenuated vaccines: Weakened forms of the pathogen.
    2. Inactivated vaccines: Killed pathogens.
    3. Subunit vaccines: Only specific antigens are included, not the entire pathogen (e.g., mRNA vaccines).

Effect of HIV on Immune System

- HIV targets helper T cells, leading to their depletion and resulting in AIDS (acquired immune deficiency syndrome).