Lab Safety and Techniques Overview

Eating in the lab: False

Bringing food in the lab: False

Dispose gloves in Trash bin: True

Dispose glass in Trash bin: False (should be disposed in a designated glass disposal container)

Contaminated slides to biohazard: True

Masks prevent aerosolized bacteria: False (masks provide barrier protection, but their effectiveness against aerosolized bacteria depends on the type of mask and particle size)

Aseptic technique requires masks: True (often used to prevent contamination from breath)

Killing samples: Boiling in water True

5 min needed for boiling: True

Spills: Pick up immediately

Magic number for multiples: False

Bunsen burner utilizes Phosphorus: False (it utilizes gas, typically natural gas or propane)

Soap used to clean slides before staining: True

Sterilization with outer cone tip: True

Aseptic technique prevents contamination: True

Pure cultures: One species only: True

Mixed cultures for biochemical properties: True

Solid Agar appearance: False (Solid agar usually appears opaque or translucent, not fully clear like a liquid)

Organisms show motility via Brownian motion: False (Brownian motion is random movement of particles suspended in a fluid resulting from their collision with the fast-moving molecules in the fluid; true motility is directed movement)

Dual cone system: Coldest inside flame: False (the inner cone is generally hotter than the outer cone)