DNA replication

So this is gonna be our Dna, (twist arms) start with supercoiling and we’re gonna (stop arms) use topoisomerase to relax the supercoiling then we’re gonna unwind (Shake arms and unwind them) and and unzip (spread arms) using helicase (shake arms up and down) then we’re gonna keep the two strands apart using what, SSB (move head, arms side to side) then we need to lay down primer because we have a leading (shake right hand) strand and we have a lagging (shake left hand) strand and the leading strand (shake right hand) is considered a leading strand because it’s the 3-prime end of the sugar, right, whereas the lagging (shake left hand) strand is the 5-prime so we’re gonna use RNA primase (shake left hand) and lay down primer (hit right hand) one time on the leading strand and then primer primer primer (hit left hand x3) on the lagging strand repetitively then we’re gonna use DNA (swirl left hand)polymerase 3 (left hand 3)who’s gonna work off of the primer and go boom boom boom (hit right arm x3)right down our leading strand then boom boom boom boom boom (hit left arm x5) and those (point to left arm) are called Okazaki (japanese greeting) fragments then (pointer finger to thumb both hands) we need to get rid of that primer that’s laid down so(back to arms spread, swirl left arm) we’re gonna use DNA polymerase 1 (left hand #1) we only have one primer (point to palm of right hand) to remove here on our leading strand so we go primer (touch right hand with #1) then we go primer primer primer (touch left hand with #1 x3) now we need to close the phosphate sugar backbone to seal it so we’re going to use ligase (elbows bounce down) then these double (both hands grab “pole” and go down and up) stranded DNA and this is called semi-conservative replication because (go back to arms spread) we have an old strand and a new strand these are gonna twist (hands point out) up and form (wrists together above head) sister (shake head and arms side to side) chromatids held (arms go up and down) together by a centromere and they’re gonna separate during anaphase (separate wrists and hands go to side)