MEDCHEM_02_2425

Page 1: Introduction

  • Medicinal and Pharmaceutical Chemistry - MEDCHEM.2 Chirality II

  • Focused on the Assignment of R- and S- configurations

  • Presenter: Dr. Declan Gaynor

Page 2: Recommended Reading

  • Organic Chemistry with Biological Applications (3rd Ed) by John McMurry

    • Chapter 5:

      • Section 5.4 - Pasteur’s discovery of enantiomers

      • Section 5.5 - Sequence rules for specifying configuration

    • Chapter 21:

      • Section 21.1 - Classifying carbohydrates

      • Section 21.2 - Representing carbohydrate stereochemistry: Fischer Projections

Page 3: Learning Outcomes

  • Distinguish between 3-D drawings and 2-D Fischer projections

  • Name Fischer projections using the Cahn-Ingold-Prelog system of nomenclature

  • Assign absolute configuration (R or S) from 3-D drawings or Fischer projections

Page 4: Stereoisomers

  • Definition: Differ by their 3-D arrangement of atoms in space

  • Representation: Bold wedge = towards viewer; Dashed wedge = away from viewer; Solid line = in the plane of the screen

  • Example: 2-Chloro-2-hydroxyacetic acid

  • Interaction with chiral molecules influenced by tetrahedral carbon arrangement

Page 5: Enantiomers

  • Defined as stereoisomers that are non-superimposable mirror images

  • Physical properties: Identical melting point, boiling point, density, etc.

Page 6: Optical Activity of Chiral Molecules

  • Optical Activity: Chiral molecules rotate polarized light

    • Clockwise = dextrorotatory = (+)

    • Anti-clockwise = laevorotatory = (-)

  • Enantiomers rotate light in opposite directions but by the same magnitude

Page 7: Example - Lactic Acid

  • Different enantiomers rotate plane-polarized light oppositely

  • Configuration categorized as (R) or (S) does not correlate with rotational direction

    • (+)-lactic acid:

      • Melting point = 53 °C, [𝛼]25 = +3.33 (H2O)

    • (-)-lactic acid:

      • Melting point = 53 °C, [𝛼]25 = -3.33 (H2O)

Page 8: Assigning (R) and (S)

  • Absolute configuration indicates spatial arrangement of atoms

  • Use Cahn-Ingold-Prelog rules for configuration assignment

Page 9: Cahn-Ingold-Prelog Rules

  1. Locate the chiral carbon (indicated by *)

  2. Assign priority (1 to 4) based on atomic numbers:

    • Higher atomic number = higher priority

Page 10: Priority Assignment

  • Higher atomic number corresponds to higher priority

  • Visual representation of increasing priority

Page 11: Identical Atoms in Priority Assignment

  1. For identical atoms, consider the next bonded atom in determining priority

  • Continue until a distinction is found

Page 12: Handling Multiple Bonds

  1. For multiple bonds:

    • Double bonds take precedence over single bonds

    • Prioritize based on overall structure

Page 13: Further Rules for Assigning Priority

  • Highest priority items considered first in the assignment process.

Page 14: Assigning Enantiomer Configuration

  1. Rotate molecule so lowest priority group is in the back

  2. Draw arrow from highest to lowest priority:

    • Clockwise = (R) enantiomer

    • Anti-clockwise = (S) enantiomer

Page 15: Orientation Guidance

  • Visualization of lowest priority group orientation

  • Tip: Think of positioning like a steering wheel during assignment

Page 16: Orientation Methods

  • Steps on how to orientate for configuration assignment

Page 17: Example Assignment of Configuration

  • Rotate so that the lowest priority group is away from view

  • Assign clockwise or anti-clockwise to derive R or S

Page 18: Example 2 - Assigning Configuration

  • Similar procedure as above

Page 19: Configuration Assignment Steps

  • Maintain lowest priority group orientation to distinguish S and R

Page 20: Final Steps for R and S Assignment

  • Confirm orientation to assign correct configuration

Page 21: Summary of R and S Assignment

  • Last orientation adjustments for configuration assessment.

Page 22: Double-Switch Method

  • Double-Switch Trick: Helps in manipulating structure on paper

  • Switching lowest group puts it in the appropriate position

  • Effective in notation without altering stereochemistry

Page 23: Double-Switch Variations

  • Indicating the flexibility in application of the double-switch technique

Page 24: Exercises & Practice

  • Opportunities to practice R & S configuration assignment

Page 25: Quick Manipulation Instructions

  1. Ensure proper positioning of groups for assignment

  2. Visualize manipulations effectively for accurate configuration assessment

Page 26: Carousel Method Application

  • Details on applying carousel method for configurations

Page 27: Fischer Projections

  • Representation of 3-D molecules as flat structures

  • Crossed lines illustrate tetrahedral z orientation

Page 28: Fischer Projection Technique

  • Expand on the 3-D mapping as flat for molecular models

Page 29: Fischer Projection Rotation

  1. Viewing manipulation strategies for Fischer projections

  2. Clarifying allowed 180° rotations vs illegal 90° rotations

Page 30: Fischer Projection Adjustments

  • Discuss allowed manipulations while maintaining correct stereochemistry

Page 31: Assigning R and S via Fischer Projections

  • Step-by-step for priority assignment followed by manipulations

Page 32: Conclusion & Contact Information

  • Thank you for participating!

  • For further information:

    • Dr. Declan Gaynor

    • Email: dgaynor@rcsi-mub.com