SDS-PAGE

SDS-PAGE

• SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide gel electrophoresis.

• It's a technique used to determine the proteins present and associated with a membrane.

• Membrane fragments are loaded into wells on a gel.

• As the fragments migrate through the gel, the proteins are separated based on their molecular weights.

• Proteins are denatured so they flow through the gel based on size, unaffected by folding.

• Known standards are run alongside the samples.

• By comparing the sizes of separated proteins to the standards, the proteins present in the sample can be identified.