Magnification and calibration

Resolution = the ability to distingiush between two entities (points)

Magnification = how many times larger the image is than the actual size of the object.

Resolution is limited by the diffraction of light.

Magnification = image size / actual size (think MIA)

Step by step on how to calibrate the eyepiece graticule:

step 1:

Adjust the stage and resolution to allow the stage micrometer to be in line, at 0, with the eyepiece graticule.

Step 2:

Work out the size/how many divisions in the micrometer. Count how many graticule divisions are in the micrometer.

Step 3:

Length of 1 eyepiece division = length stage micrometer / number of divisions.