Page-by-page study notes: Wilkins Ch. 7 Infection Control (Clinical Procedures)
Page 1
- Topic: Wilkins Ch. 7 Infection Control – Clinical Procedures
- Focus: PART 1 – Preparing the Operatory
- Goals introduced (overall): reduce pathogenic microorganisms, break the chain of cross-contamination, and apply standard/universal precautions.
Page 2
- Reduction of pathogenic microorganisms: reduce to a level that the body’s resistance mechanisms can handle.
- Break the chain of cross-contamination: prevent transmission between sources, objects, and people.
- Standard precautions: also referred to as Universal Precautions.
Page 3
- Ventilation fundamentals:
- Vents should be free from clutter.
- Air flow should go from clean to less clean.
- Change and maintain filters regularly.
- Vents cleaned and maintained.
- Run ventilation continuously during business hours and for 2 hours after closing.
Page 4
- Ventilation enhancements:
- Portable HEPA filter placed near the patient, away from the clinician.
- Upper-room ultraviolet germicidal irradiation (UVGI) units can be used with ventilation units to inactivate microbes.
- Bathroom exhaust fan should run continuously to promote airflow and dilution.
Page 5
- Patient placement strategies:
- Individual treatment rooms are best; open floor plans are less ideal for infection control.
- Position patients 6 ft apart when possible.
- Use physical barriers between patient chairs.
- Place patients parallel to the direction of air flow when possible.
- Position patient heads near air return vents when possible.
- When feasible, position patients’ heads away from open hallways, especially during aerosol-generating procedures.
Page 6
- Preprocedural measures:
- Have patient rinse with an antiseptic preprocedural rinse, especially before injections and scaling.
- Have patient brush; 0.2% chlorhexidine rinse provides lower bacterial counts for >60 minutes.
Page 7
- Definition:
- “The spread of microorganisms from one source to another.”
- Examples include: patient saliva to a dental instrument to Hygienist’s hand when gloves are not used or gloves/hand hygiene fail.
Page 8
- Definitions:
- Disinfect: To destroy pathogenic microorganisms (mo's).
- Sterile: Free from viable microorganisms.
Page 9
- Decontaminate: Use physical or chemical means to remove, inactivate, or destroy pathogenic microorganisms on a surface or item so they are no longer capable of transmitting disease; render safe for handling.
- Disinfectant: An agent (chemical or sometimes physical such as x-rays or ultraviolet light) that destroys microorganisms but may not kill bacterial spores.
Page 10
- Sterilization: Process by which all forms of life, including bacterial spores, are destroyed by physical or chemical means.
Page 11
- Surfaces and equipment features that are easy to clean:
- Smooth, uncluttered surfaces.
- Removable hoses; avoid non-retractable, hard-to-clean hoses.
- Autoclavable or disposable syringe tips.
- Hand pieces with anti-retraction valves and autoclavable.
- Use of barriers where possible.
Page 12
- Treatment room supplies and features (summary list):
- Clinician's stool: foot controls, easy-clean surface, no seams, barrier cover.
- Light: autoclavable handle or barrier cover.
- Sharps disposal: appropriate container.
- Sink: stainless steel; electronic or foot controls; deep for washing up to elbows.
- Biohazard waste: foot control; sealable liner.
- Waste: large opening; heavy-duty liner.
- Chair: foot controls; easy-clean surface; no seams.
- Unit: removable hoses, straight (not coiled); easy-clean surfaces; autoclavable handpieces.
- Floor: smooth, easy to clean, nonabsorbent; no carpeting.
- Note: Copyright 2013 Wolters Kluwer Health; Lippincott Williams & Wilkins.
Page 13
- Treatment Room Feature 1: Dental Chair
- Controls foot-operated (or must be covered if not).
- Surfaces should be seamless and easily cleaned.
Page 14
- Treatment Room Feature 2: Light
- Foot-activated switches or sensors; if not available, use barriers.
- Page 14 also mentions: “Petion Cane” (likely a stray/placeholder item in the transcription).
Page 15
- Treatment Room Feature 3: Clinician's Stool
- Smooth surface; foot-operated controls.
Page 16
- [Page 16 appears blank in transcript; no notes to add here]
Page 17
- Additional treatment room features:
- Foot-operated or sensor-controlled fixtures.
- Sink should be wide and deep enough for effective hand washing.
- There should be a separate sink for hand washing and instrument care.
Page 18
- Treatment Room Features continued:
- Floors: No carpeting.
- Supplies: All sterilizable or disposable.
- Waste: Receptacle with large opening; separate sharps container; small biohazardous receptacle.
Page 19
- Visual cue question: What is wrong with this picture? When polishing a patient’s teeth, the splatter zone extends to about 6 feet.
Page 20
- Common issues in treatment rooms:
- Cluttered countertops.
- Exposed supplies (gloves, masks).
- Same sink used for sterilization and hand washing.
- Same countertop for clean and dirty instruments.
- Soda in treatment area (implies food/beverages in clinical space).
Page 21
- Disinfecting Surfaces: Topic introduction.
Page 22
- Surfaces requiring disinfection (examples):
- Dental light handles
- Chair switches
- Dental radiograph equipment
- Chairside computer keyboards
- Reusable containers of dental materials
- Drawer handles
- Faucet handles
- Countertops
- Pens
- Telephones
- Doorknobs
- Additional items listed (incomplete text in transcript)
Page 23
- Categorization by biocidal activity:
- High level: Inactivates spores and all forms of bacteria, fungi, and viruses; may be a disinfectant or sterilant depending on time.
Page 24
- Intermediate level: Inactivates all forms of microorganisms but does not destroy spores.
- Low level: Inactivates vegetative bacteria and certain lipid-type viruses; does not destroy spores, TB bacilli, or nonlipid viruses.
Page 25
- Chemical disinfectants: Uses include environmental surface disinfection and dental lab impressions/prostheses.
Page 26
- Disinfection depends on contact of the solution with surfaces: Items must be cleaned and dried for the agent to work.
- Action of the agent may be altered by foreign matter.
Page 27
- EPA approval and labeling requirements for disinfectants:
- Tuberculocidal, bactericidal, virucidal, and fungicidal.
- Label must state: shelf life, use life, reuse life; directions for activation; storage; directions for use; disposal instructions; warnings.
Page 28
- Desired properties of disinfectants:
- Broad spectrum
- Fast acting
- Not affected by physical factors
- Non-toxic
- Surface compatibility
- Residual effect on treated surfaces
- Easy to use
- Odorless
- Economical
Page 29
- Common chemical disinfectants mentioned:
- Gluteraldehydes: should not be used as a surface disinfectant.
- Chlorine compounds
- Iodophors
- Combination phenolics
Page 30
- High-level disinfectants:
- Can be used as cold disinfectants.
- Limitations:
- Caustic to skin
- Irritating to eyes
- Corrosive to some metals
- Items need to be rinsed in sterile water
- Toxic fumes; therefore not used as a surface disinfectant
Page 31
- Sodium hypochlorite; chlorine dioxide:
- Use life of 1 day; must be mixed daily.
- Use distilled water; improves stability.
- Can harm eyes; corrosive; odor; economical.
Page 32
- Povidone-iodine:
- Broad spectrum.
- Can discolor.
- Mix 1 part iodophor to 213 parts water.
- Mixture color changes from amber to clear as activity declines.
Page 33
- Combination phenolics:
- Broad spectrum.
- Lower concentrations used as surface disinfectants.
Page 34
- Statement-based question: High-level disinfectants inactivate spores and all forms of bacteria, fungi, and viruses (Statement A). Intermediate-level disinfectants inactivate all forms of microorganisms but do not destroy spores (Statement B).
- Answer format: A) both correct; B) both correct; C) A correct, B incorrect; D) A incorrect, B incorrect. (From transcript: select the correct option—A is correct and B is correct.)
Page 35
- Eyewash station usage: How long should a person use an eyewash station? Answer: 15minutes
Page 36
- Chemical disinfection vs sterilization:
- Used only for items that cannot be sterilized.
- Chemical may disinfect in 10−30min but may take 10hours to sterilize.
Page 37
- Chemical Sterilants (Immersion):
- Disadvantages: Cannot verify sterilization; difficult to store instruments; time constraints; BIOCIDE.
- Chemical sterilant requires the longest time to complete cycle.
Page 38
- Preparation of the Treatment Room – Objectives:
- Control disease transmission
- Increase working efficiency
- Create an atmosphere of cleanliness and orderliness
- Increase patients’ confidence
- Maintenance of equipment
Page 39
- Hand hygiene and instrument handling:
- Hand contacts: Touch only what you need; plan ahead; use paper towel barrier.
- Sterilize items: Critical and semi-critical items need to be sterilized.
Page 40
- Surface category definitions and examples:
- Critical: Penetrates soft tissue or bone; sterilize or use disposable. Examples: needles, curettes, explorers, probes.
- Semicritical: Touch intact mucous membrane and oral fluids; does not penetrate; high-level disinfection when sterilization cannot be used. Examples: radiographic biteblock, ultrasonic headpiece, amalgam condenser, mirror.
- Noncritical: Do not touch mucous membranes (only contact unbroken epithelium). Cleaning and TB intermediate-level disinfection. Examples: light handles, some x-ray machine parts, safety eyewear.
Page 41
- Disposable items: Use whenever possible.
- Cover items that can be covered.
- Chemical disinfection as a general approach.
Page 42
- Two key actions determine disinfectant effectiveness:
- Physical rubbing and removal of contaminated material.
- Chemical inactivation of living microorganisms.
Page 43
- Flushing protocols:
- Flush lines for 2 minutes at the beginning of the day.
- Flush for 30 seconds before and 30 seconds after each patient appointment.
Page 44
- BARRIERS! (likely a visual or branding slide)
Page 45
- PART 2: Sterilizing Instruments – LAB 3
- Focus: The sterilization and instrument management portion of universal precautions.
Page 46
- Universal precautions in instrument management:
- All critical and semi-critical supplies should be sterilizable or capable of being sterile.
- A planned program for instrument management is essential for safe practice.
Page 47
- Personal protective equipment (PPE) and handling:
- Clinician should wear heavy utility gloves, mask, safety glasses, and a cover gown.
- Ideally, instruments should be kept in a cassette with minimal handling.
Page 48
- Instrument cleaning workflow (therm disinfection or ultrasonic):
- Step: Instrument cleaning (therm Disinfection or Ultrasonic)
- Rinse and dry
- Clinical use
- Package
- Seal
- Sterilize
- Store packages
Page 49
- Pre-cleaning step:
- Does NOT sterilize; only cleans.
- Safer than manual cleaning.
- Benefits: increased efficiency, decreased danger to clinician, improved disinfection effectiveness, fewer aerosols, penetrates into all areas, removes tarnish.
Page 50
- Three basic methods to clean instruments:
1) Instrument washer / thermal disinfector
2) Ultrasonic processing
3) Manual cleaning
Page 51
- Instrument Washer / Thermal Disinfector:
- Uses high-velocity hot water and a detergent.
- Higher temperature provides disinfection.
- Disinfection allows cassettes to be handled during packaging.
Page 52
- Ultrasonic Processing (not a substitute for sterilization):
- It is a cleaning process, not a sterilization method.
- Advantages: increased efficiency; reduced danger to clinician.
Page 53
- Ultrasonic Processing procedure:
- Do not overload the unit.
- Dismantle instruments.
- Process for correct amount of time.
- Drain, rinse, and air dry.
Page 54
- Manual Cleaning procedure:
- Last resort option.
- Wear heavy-duty gloves.
- Dismantle instrument.
- Use long-handled brush; brush away from body.
- Rinse thoroughly; air dry.
Page 55
- Basic steps in the recirculation of instruments – Packaging step (Part I):
- Purpose: Prevent contamination; storage method.
- (Figure references appear; content suggests packaging step concepts.)
Page 56
- Packaging step 2 – Arrangement:
- Preset cassettes or trays.
- Date packages.
- Clear packaging for easy identification.
Page 57
- Packaging step 3 – Preparation:
- Use sturdy wrap.
- Must allow steam or chemical vapor to penetrate.
Page 58
- Seal details:
- Indicator tape: Do not use pens, paper clips, or other metal fasteners that poke holes in packaging.
- Use only if the package is self-seal and wrap has built-in indicators.
- Chemical indicator: Used to seal packages unless wrap has built-in indicators.
- Change in color indicates temperature has been reached; does not guarantee sterility by itself.
Page 59
- Sterilization methods (approved):
- Steam under pressure (autoclave)
- Dry heat
- Chemical vapor
- Immediate-use steam sterilization (flash)
- Chemical (cold) sterilization (not recommended)
- The chosen method must provide complete destruction of all microorganisms, viruses, and spores.
Page 60
- Tests for Sterilization (three tests):
- External chemical indicator
- Internal chemical indicator
- Biological monitor
- These tests verify that the sterilizer is functioning properly.
Page 61
- External Chemical Indicator:
- Used to seal the package and indicate that the autoclave temperature has been reached.
Page 62
- Internal Chemical Indicator:
- Color change assesses instrument exposure to temperature and steam for the required time.
Page 63
- Biological Monitor:
- Tests that the autoclave is functioning properly.
- Weekly testing recommended.
- Refer to Table 7-2 (p. 109) for complete list in the source.
Page 64
- Sterilization indicators by method:
- Steam autoclave: Geobacillus stearothermophilus (vials/strips/ampules).
- Dry heat oven: Bacillus atrophaeus (formerly Bacillus subtilis) strips.
- Chemical vapor: Geobacillus stearothermophilus strips.
- Ethylene oxide: Bacillus subtilis strips.
Page 65
- Causes of incomplete sterilization:
- Inadequate preparation.
- Misuse of equipment.
- Inadequate maintenance.
Page 66
- Moist Heat (steam under pressure):
- Causes coagulation of proteins.
- Autoclave: may be used for all materials except oils, waxes, and powders that are impervious to steam or cannot be subjected to high temperatures.
Page 67
- Types of Autoclaves:
- Gravity displacement: self-generated steam forces air out; steam enters to penetrate packages.
- High-Speed Prevacuum (prevacuum): pump removes air for faster steam penetration; examples include Statim.
Page 68
- Table 6-1 – Methods for Sterilization (summary):
- Steam under pressure (autoclave): Gravity displacement – Time: 15−30min; Temperature: 250∘F=121∘C; Pressure: 15 psi.
- Prevacuum: Time: 3.5−10min; Temperature: 270∘F=132∘C.
- Dry heat: Time: 120min; Temperature: 320∘F=160∘C.
- Unsaturated chemical vapor: Time: 20min; Temperature: 270∘F=132∘C; Pressure: 20−40 psi.
Page 69
- Important autoclave note:
- Temperature must remain 121∘C at 15 psi for 15 minutes after reaching proper pressure and temperature.
- Pack loosely to allow steam to reach all instruments.
Page 70
- Sterilization concept:
- Sterilization is achieved by heat and moisture (no air).
- Discharge occurs in a downward direction.
- Pressure is used to attain the heat, not to sterilize by itself.
Page 71
- Sterilization outcomes and scope:
- All microorganisms, spores, and viruses are destroyed.
- A wide variety of materials can be treated.
- Most economical method uses distilled water.
Page 72
- Moist Heat – Disadvantages:
- May corrode carbon steel.
- Not suitable for oils and powders.
Page 73
- Dry heat: Action and intended use:
- Oxidation of molecules leads to organism death.
- Static air vs forced air options explained.
- Primarily used for materials that cannot be safely sterilized with steam (e.g., corroding/rusting items).
- Useful for oils and powders; small metal instruments in special containers.
- Typical cycle: 160∘C=320∘F for 2 hours or 170∘C=340∘F for 1 hour (time starts after target temp is reached).
Page 74
- Dry heat – Advantages and Disadvantages:
- Advantages: good for items that cannot withstand steam; less corrosion; suitable for sharp instruments if temp is maintained.
- Disadvantages: long exposure time; higher temp is crucial; penetration is slow and uneven.
Page 75
- Chemical Vapor (aldehyde-based) sterilization notes:
- Involves alcohol and formaldehyde vapors under pressure.
- Used for low-melting plastics, liquids, and heat-sensitive handpieces.
Page 76
- Chemical Vapor continued:
- Microbial and viral destruction results from permeation of heated formaldehyde and alcohol.
- Not suitable for materials that can be altered by these chemicals or cannot withstand heat.
Page 77
- Chemical Vapor product/method names (examples):
- VAPO-STERIL or similar solutions.
- Operates at around 132∘C under pressure.
Page 78
- Chemical Vapor advantages:
- Corrosion and rust-free.
- Relatively short cycle.
- Easy operation.
Page 79
- Chemical Vapor disadvantages:
- Requires adequate ventilation.
- Slight odor.
- Refill depends on use; typically every 30 cycles.
- Packages cannot be tightly wrapped or tightly sealed.
Page 80
- Ethylene oxide (ETO) sterilization:
- Used in hospitals and larger clinics; most materials sterilizable including plastics and rubber.
- Low-temperature process; effective for heat-sensitive items.
- Very long cycle time depending on temperature and concentration.
- Typical duration: approx. 10−16 hours.
Page 81
- [Page 81 appears blank in transcript; no notes to add here]
Page 82
- Ethylene oxide – Advantages:
- Can sterilize many materials including plastics and rubber.
- Low temperature operation.
Page 83
- Ethylene oxide – Disadvantages:
- High equipment cost.
- Requires good ventilation.
- Increased time required.
- Items must be aired out for several hours after sterilization.
Page 84
- Flash Sterilization (Immediate-use steam sterilization):
- Rapid steam heat sterilization used for unwrapped instruments for immediate use.
- Shorter sterilization times.
- Use only when urgent; not recommended for items requiring biological spore test results (e.g., implants).
- Care: follow manufacturer settings; monitors/indicators should be used and checked each cycle.
- Items used for immediate use should not be stored.
- Biologic monitoring is not possible in flash sterilization.
- Beware of potential contamination during packaging; shelf life may vary by environment (up to 1 year in some cases).
Page 85
- Practical cautions for flash sterilization:
- Do not store items sterilized in flash mode.
- Monitor indicators per cycle.
Page 86
- Summary table (reiterating sterilization methods and parameters):
- Moist Heat – Steam Under Pressure: Gravity displacement – Time 15−30min; Temp 250 ∘F (121°C); Pressure 15 psi.
- Moist Heat – Steam Under Pressure: Prevacuum – Time 3.5−10min; Temp 270 ∘F (132°C).
- Dry Heat – Time 120min; Temp 320 ∘F (160°C).
- Unsaturated Chemical Vapor – Time 20min; Temp 270 ∘F (132°C); Pressure 20−40psi.
Page 87
- Packaging and storage specifics:
- Unpackaged instruments are sterile only momentarily.
- Packaged items can be stored for several months to 1 year, depending on storage method.
Page 88
- Summary – Standard procedures (patient factors):
- Comprehensive health history.
- Preprocedural rinse.
- Protective eyewear for patients.
Page 89
- Clinical preparation and operations:
- Run water lines for 2 minutes before procedures.
- Disinfect environmental surfaces.
- Apply barriers.
- Use sterilized instruments.
- Factors for dental team:
- Use PPEs.
- Thorough hand hygiene.
- Do not contaminate or cross-contaminate.
Page 90
- Treatment factors:
- Hypodermic needles: Safe recapping methods; dispose in puncture-resistant sharps container; dispose of partially used anesthetic carpules.
- Removal of oral prostheses: Wear gloves to receive prostheses from patient.
Page 91
- Post-treatment procedures:
- Use heavy puncture-resistant gloves to handle instruments.
- Disinfect, clean, and prepare instruments for sterilization.
- Disinfect eyewear/face shield for patient and dental team member.
- Sterilize instruments.
Page 92
- Cleaning face after exposure:
- Should occur after an incident and several times a day.
- Smoking and eating are banned from treatment areas.
Page 93
- After an exposure incident (steps):
- Immediately wash the wound with soap and water.
- Report to designated official and complete an incident report as required.
- Follow clinic/institution procedures and the most recent guidelines by the US Public Health Service.
- Important note: Do not squeeze the wound to make it bleed.
Page 94
- Final takeaway: “Only YOU can stop the spread of infection!”
- Emphasizes personal responsibility in infection control and adherence to procedures.