Lecture on Quantum Physics and Electron Microscopy
Lecture Overview
- Lecture № 11 Topics:
- Quantum physics
- Electronic microscope
- Thermal radiation
- Solar radiation
- Infrared and ultraviolet diagnostics and therapy
- Types of optical transitions
- Luminescence
- External and internal photoelectric effect
Photons and de Broglie Waves
- Photons:
- Definition: Particle-like quanta of electromagnetic radiation
- Properties: Travel at the speed of light (c) with momentum (p) and energy (W)
- Relationships:
- W = h
u - rac{
u}{rac{1}{
u}} = rac{hc}{h
u} - p = rac{h}{
u}
- de Broglie Hypothesis:
- Proposed by Louis de Broglie in 1923
- Statement: Particles of matter, such as electrons, can exhibit wave-like properties.
- Wave wavelength for a particle with momentum (p):
- rac{mv}{h}
- de Broglie Wavelength:
- Experimental confirmations since 1927 show protons, neutrons, atoms, and molecules exhibit wave-like behavior.
- Differences from Classical Waves:
- de Broglie waves are attributed to microparticles.
- Do not have counterparts in classical physics.
Experimental Verification of de Broglie's Hypothesis
- Experiments by Tartakovsky and Thomson:
- Demonstrated diffraction patterns with light, atoms (H, H2, He), protons, and neutrons.
- Key Equations:
- Optical path difference determined by:
- ext{Δ} = 2AB = 2d ext{sin} heta
- Wulff–Bragg Condition:
- 2d ext{sin} heta = n ext{λ}; n = 1, 2, …
- Noteworthy Results:
- Experiments in 1927 showed that electrons behave like waves.
- Wavelength Calculation Example:
- For U = 100 kV,
- ext{λ} ext{approx} = 4.10^{-12} ext{m} = 400 ext{pm}
Electron Microscopes (EM)
Working Principle
- Functionality:
- EMs utilize electron beam interaction with a sample to gather structural and compositional information.
- Components:
- Electron gun generates electrons.
- Two condenser lenses focus the electron beam on the specimen.
- Accelerating voltage between tungsten filament and anode (typically 100 kV to 1000 kV).
- Sample Preparation:
- Specimens must be ultra-thin (20-100 nm thickness), at least 200 times thinner than those in optical microscopes.
- Electron Interaction:
- Electrons scatter based on differing density or refractive index within the sample.
- Denser areas appear darker as fewer electrons reach the imaging screen; lighter areas indicate transparency.
Types of Electron Microscopes
- Categories:
- Transmission Electron Microscope (TEM)
- Scanning Electron Microscope (SEM)
- Field Emission Scanning Electron Microscopy (FESEM)
- Reflection Electron Microscopy (REM)
- Scanning Tunneling Microscopy (STM)
Transmission Electron Microscopy (TEM)
Overview
- Purpose:
- Used for imaging thin specimens such as tissue sections and molecular structures.
- Operation Modes:
- Imaging Mode: Obtains images of samples.
- Diffraction Mode: Determines crystal orientation and cell structure.
- Sample Thickness:
- Ultra-thin sections < 100 nm, placed on a copper grid around standard size (3.05mm diameter).
- Voltage Ranges:
- Ordinary (50-100 kV) and high voltage (up to 3 MV).
- Imaging Capabilities:
- High resolution with maximum magnification
- Can image carbon atoms (0.089 nm) and silicon atoms (0.078 nm) at magnifications > 50 million times.
Scanning Electron Microscopy (SEM)
Characteristics
- Operation:
- Based on secondary and backscattered electron emission.
- Reveals surface morphology, chemical composition, and crystalline structure.
- Utilizes lower voltage than TEM (30-40 kV).
- Image Formation:
- Secondary electrons contribute surface imaging with high resolution < 1 nm.
- Backscattered electrons provide deeper insights (lower resolution) for multi-phase compositions.
- Advantages:
- Greater depth portrayal; provides 3D structure representation.
Infrared and Ultraviolet Radiation
Infrared Diagnostics and Therapy
- Infrared Radiation Characteristics:
- Wavelength: 0.8 µm - 1000 µm.
- Body temperature (37 °C): λmax ≈ 9.5 µm.
- Therapeutic Applications:
- Traditional and modern infrared lamps (e.g., Solux lamps) for various treatment methodologies.
Ultraviolet Diagnostics and Therapy
- Ranges:
- UV Spectrum: 10 nm - 400 nm (e.g., UV-A, UV-B, UV-C)
- Health Implications:
- Overexposure risks like skin cancer, cataracts.
- Therapeutic doses beneficial for vitamin D production.
Quantum Optical Phenomena
Thermal Radiation
- Definition:
- EM radiation from the thermal motion of particles.
- Stefan-Boltzmann Law:
- E = σT^4 where σ represents the Stefan–Boltzmann constant.
- Planck’s Law of Blackbody Radiation:
- E = hν where h is Planck's constant and ν is frequency.
Types of Optical Transitions
Quantum Transitions
- Absorption:
- Photon absorbed matching energy separation between quantized states.
- Emission Types:
- Spontaneous and stimulated emissions.
- Stokes' Law:
- Emission occurs at longer wavelengths than absorption.
Luminescence
Types of Luminescence
- Forms:
- Photoluminescence, electroluminescence, and chemiluminescence.
- Applications:
- Medical diagnostic tools using fluorescence analysis for tracking substances in biological tissues.
Photoelectric Effect
External Effect
- Definition:
- Emission of electrons from materials when excited by electromagnetic radiation.
- Key Points:
- Electrons emitted are called photoelectrons.
- Occurred study by Hertz & Stoletov – emission from metals conditions.
- Conductivity Implications:
- Photoconductivity in semiconductors through exposure to light.
Solar Cells
Structure and Working
- Construction:
- Silicon photovoltaic cells with p-n junctions for light detection and power supply.
- Production Data:
- Typical solar cell output of 1 watt with efficiency of ~15% based on duration of exposure.