pH and Enzymes - Core Practical

  1. Put a drop of iodine solution of every well of the spotting tile
  2. Put beaker of water over bunsen burner and heat it until it is 35 degrees
  3. Use syringe to add amylase solution and buffer solution to a boiling tube
  4. Put boiling tube inside beaker of water
  5. Add starch to the tube
  6. Mix the contents and start a stopwatch
  7. Use pipette to take a sample of the tube every 10 seconds and put a drop in a well
  8. When the iodine is brown starch is no longer present
  9. Keep repeating until all wells are filled