Connection btwn ER-Golgi-PM and Organelles

HSPs - chaperones, released in response to heat shock

Laemelli - invented phage gels 1972

two types of transport:

vesicular

non-vesicular

ER → cisGolgi: COPII-coated vesicle

transGolgi→ out: COPII-coated vesicle

Golgi → ER: COPI-coated vesicle

transGolgi → lysosome: ends in mannose-5-phosphate and picked up by mannose-5-phosphate receptor

  • folic acid reductase → lysosome using consensus sequence and terminal mannose

GFP - cats + radiation

plants do the same 👍

glycosylation - N-linked ER and O-linked Golgi

  • unusual negatively charged sugars

ER - connection throughout the cell

  • 1% cytoplasm

  • peroxisomes attached to ER

  • Golgi also attached

I3P - binds to VDAC - calcium entrance into mitochondrial inter-membranous space

ER stress = inflammation

  • inflammmasome - releases cytochrome C

  • apoptosasome -

  • necrosome -

Mitochondria-associated ER membranes

  • lipid synthesis/transfer

  • Ca2+ transfer and apoptosis

  • mitochondrial dynamics (fission)

  • autophagy

  • ROS formation and ER stress

  • Inflammation

cardiolipin - unique to inner (?) mitochondrial membrane

plasmalogens - peroxisome phospholipid that is finished in ER