Digestive Day 4 and Review
Digestion of Carbohydrates
Starch Definition: A polysaccharide which is one of the main forms of stored energy in plants.
Digestion Process:
Starts in the mouth with the enzyme amylase.
Starch is first broken down into polysaccharide, then into disaccharide, and finally into monosaccharide (glucose).
Use of cracker as an example:
When chewed, it becomes mushy due to enzymatic action from salivary amylase.
Limitations in the Stomach:
The acidic environment of the stomach (HCl) denatures amylase and pepsin digests it.
Thus, only about 50% of carbohydrate digestion occurs before entering the small intestine.
Role of the Pancreas and Small Intestine
Pancreatic Juice:
Contains pancreatic amylase which continues the breakdown of carbohydrates.
Brush Border Enzymes:
Important enzymes in the small intestine include:
Dextranase and Glucoamylase: Hydrolyze oligosaccharides into monosaccharides.
Maltase: Hydrolyzes maltose.
Sucrase: Hydrolyzes sucrose.
Lactase: Hydrolyzes lactose.
Final product of digestion is monosaccharides which can be absorbed.
Absorption of Monosaccharides
80% of absorbed sugar is glucose.
Transport Mechanisms:
Glucose and galactose are absorbed via carrier proteins.
Fructose is absorbed via facilitated diffusion (requires a carrier, but not a transporter).
Osmolarity:
Increased osmolarity in the intestines draws water into the intestinal walls, enhancing absorption.
Transport Pathway:
Monosaccharides are transported into the bloodstream via the superior mesenteric vein to the hepatic portal vein, leading to the liver.
Lactose Intolerance
Definition: Lack of lactase enzyme leads to undigested lactose in the large intestine, causing increased osmolarity and diarrhea due to water retention.
Statistical Insights:
About 90% of Black Americans and nearly all Asians are lactose intolerant.
Fermented products like yogurt and cheese can often be consumed as they contain pre-digested lactose.
Digestion of Proteins
Proteolytic Enzymes: Include:
Pepsin: Begins digestion in the stomach under acidic conditions, breaking bonds in proteins.
Trypsin and Chymotrypsin: Continue digestion in the small intestine after pancreatic juice neutralizes the pH.
Further Breakdown: Oligopeptides are broken down into amino acids by:
Carboxypeptidase, Aminopeptidase, and Dipeptidase: All these enzymes break peptide bonds. The conversion process allows amino acids to be easily absorbed into the bloodstream.
Lipid Digestion
Role of Lipases:
Begins with lingual lipase in the mouth but is activated in the stomach with gastric lipase.
Only about 15% of lipids are digested in the stomach.
Emulsification: Emulsifies fat globules into smaller droplets without breaking bonds.
Bile and Pancreatic Lipase: Work together to further break down triglycerides into monoglycerides and free fatty acids.
Micelle Formation:
Micelles are formed with bile salts, making absorption of lipids more efficient.
Absorption of Fats
Chylomicron Formation:
Once in the intestinal cells, free fatty acids and monoglycerides are reconstituted into triglycerides and packed into chylomicrons in the Golgi apparatus.
Chylomicrons enter the lymphatic system through lacteals before entering the circulatory system.
Complex Absorption Process: Lipids are more complex compared to carbohydrates and proteins due to their hydrophobic nature.
Nutrient Summary and Additional Nutrient Absorption
Nucleic Acids: Hydrolyzed into nucleotides, sugars, and phosphates through pancreatic nucleases and then absorbed like other energies.
Fat-Soluble Vitamins (A, D, E, K): Absorbed along with dietary fats.
Minerals: Such as sodium, iron, calcium absorbed via specific transporters:
Iron: Absorbed as Fe²⁺, inhibited by hepcidin to prevent overload.
Calcium: Absorbed in the presence of Vitamin D.
Water Absorption and Conditions Affecting Absorption
Absorption primarily occurs through osmosis, following salt absorption.
Dehydration Disorders:
Diarrhea: Too little water absorbed, leading to loose feces. Common causes include lactose intolerance.
Constipation: Excessive water absorption leads to hard stools, caused by slow fecal movement through the intestines.
Key Concepts in the Digestive System
Digestive Process Overview:
Chemical digestion starts in the mouth (for carbs) and continues in the stomach (for proteins) and small intestine (for all). Different enzymes and conditions apply through each organ.
Transport Mechanisms:
Carbohydrates and proteins are absorbed directly into blood capillaries, whereas fats are absorbed indirectly via lymphatics.
Parathyroid Hormone: Regulates calcium levels by stimulating the release from bones and enhancing absorption via Vitamin D.
Digestive Enzyme Production: Various cells produce hydrochloric acid (parietal cells), pepsinogen (chief cells), bile (liver), and lipases (pancreas).
Questions from the Lecture
What stimulates gallbladder contraction?
Answer: Cholecystokinin (CCK).
Which enzyme catalyzes carbonic acid formation in red blood cells?
Answer: Carbonic anhydrase.
Types of epithelial tissue in digestive tract:
Simple columnar predominantly in the digestive tract; stratified squamous at the entry and exit points (mouth, pharynx, anus).