Herpetology Notes
Caecilians Characteristics
Segmented
No limbs
No pelvic girdle
Tail highly reduced in size or absent
Highly reduced eye, in some cases it is covered by bone
Sensor tentacles
Compact skull (strong as it is very bony)
Relatively large teeth, two rows in upper jaw
Cloaca of males is eversible (intromittent organ), known as phalloduem
Bony Scales may be present between annular rings
60-290 vertebrae
Size range: 10.2 cm to 152 cm (5 ft)
Highly specialized for burrowing, some are entirely aquatic
Caecilian Phylogeny/zoology
Difficult to study due to political issues around the world.
Moist environments, can get to high densities but difficult to track
Tentacles
Used for picking up large organic scent molecules from surface of the environment
Small volatile scent molecules detected using olfactory cells associated with the nasal epithelium
Feeding and jaw structure
Sharp teeth and strong jaws, will do a death roll sometimes
Skull morphology
Having a big head and strong bite is great for a predator, but not so good for burrowing.
To open mouth: Contract depressor mandibulae, skull goes up, lower jaw stays in place.(think handles of bolt cutters)
To close mouth: 1) Interhyodeus muscle contracts, 2) capitis longus contracts, 3) adductor muscles contract
Subdermal scales and mucous glands
Subdermal scales embeded in skin
Body morphology: Movement and locomotion
Two sets of muscles: One set just under skin, second wrapped around spinal column
Mechanics of movement: exterior part of animal is ½ wavelength out of phase with spine. Completely unique among vertebrates.
Reciprocating locomotion: used in burrowing
Caecilian reproductive modes
All species use internal fertilization (as far as we know)
Approx 25% of species w/ free-living larval stage
25% direct development
50% viviparous
Reproduction II: Dermatophagy
Skin sloths off in a milky substance for young to eat
Reproduction III: Matorphagy
Fetal dentition: transitory non-pedicellate teeth
Juveniles graze on rapidly proliferation endometrium
Offspring may eat their mothers weight in mass every 2-3 weeks
When consumption > than proliferation, birth occurs
Salamander Characteristics
12-40 trunk vertebrae, 20 – 80 caudal vertebrae (Note: caecilians may have hundreds of vertebrae)
Limbs reduced in some (Sirens, and amphiuma)
Fertilization external or internal via spermatophore
Aquatic larval stage present or absent
Paedogenesis common
Mostly New World distribution (only one species in New World tropics
Reproduction
Majority of salamander families have internal fertilization via spermatophore
Metamorphosis
Split habitat resources:
aquatic vs. terrestrial
Larvae completely carnivorous
suction feeding mechanism
do not have pedicellate teeth
Neuromast organs: sensory and orientation Larvae lack poison glands of adult
Alteration of skeletal elements in skull
Salamander Feeding specializations
Two patterns:
Ancestral: wide spread in majority of families
Derived: only present in Plethodontids (esp. Bolitoglossines)
Urohyal may be present or absent
Circumglossus gone
Genioglossus gone
Epibranchials are really long
Parental care
Trait probably evolved independently several times in salamander lineages.
One sex guards clutch
Role of fungicides and bacteriocides
Internal retention of eggs
Occurs in only two genera of salamandrids
Two patterns:
Several eggs ovulated but only one or two eggs hatch in oviduct. Larvae consume yolk of unfertilized eggs.
1. Eggs hatch, larvae begin to consume eggs and eventually each other.
2. In both cases, young are born fully formed, up to 2/3 size of adult.
Gestation 1.5-2 yrs.
Ecological implications of cutaneous versus pulmonary ventilation
Lungless salamanders: have miniscule home ranges (e.g. 0.4 - 0.7 m2 )
Home range size decreases with body size
Lunged salamanders: long range migrations of several hundred meters to breeding ponds common (e.g. one Ambystoma tigrinum individual moved 162 m in a single night!)
Anuran Characteristics
5-9 trunk vertebrae
Several caudal vertebrae fused forming urostyle located between ilia
Skull large and broad with frontal and parietal bones fused
Pelvic girdle very long, and hind limbs much longer than forelimbs
Carnivorous as adults
Anuran Taxonomy
Highly specialized larval and adult morphologies (under largely independent selective regimes)
Age (> 200 mya)
Tremendous species diversity (e.g. 9 anuran families have only 1-3 species each)
Morphological and zoogeographic characteristics used
Tongue structure: present absent, two modes of protrusion
Pectoral girdle
Vertebrae, anatomy and number
Zoogeography (it’s a rat’s nest of relics and convergence)
Larvae: four recognizable forms
Phylogeny of anuran families
Neobatrachia: True, or “higher” frogs. Group contains 95% of extant frog species., presence of firmisternal pectoral girdle.
Pelobatoidea (Mesobatrachia): historically named because its position representing an intermediate lineage between primitive (Archaeobatrachia) and true (Neobatrachia) frogs. Shows a mosaic of derived and primitive traits. + +
Archaeobatrachia: “primitive” frogs, ribs are not fused to vertebrae, presence of arciferal pelvic girdle.
Larvae
Initially limbless, often with horny beak Surrounded by pad covered with horny denticles
Gills internal, covered by large chamber with one or two openings Most herbivorous or suspension feeders
Abdomen filled with very long spiral intestine
Metamorphosis of larvae into adults most extreme of all vertebrates
Direct development in a few species, none are neotenic
Larval Stage
Exploit high primary productivity from temporary bodies of water
Most are herbivorous or suspension feeders, a few species are carnivorous Tadpole rasps surface and food floats free
Food is drawn into mouth and particles become caught in mucous net overlaying the gills
Metamorphosis
Tadpoles have little or no bone, so bone formation must occur in adult frogs.
Changes: Horny beak falls off Palatoquadrate shortens, becomes vertically oriented while at the same time mandible elongates
Antorbital process and ethmoid cartilage fuse Kidneys: ammonia to urea Brain “re-wired”
Gene-expression differences: larvae versus adult
Reproduction
Eggs and larvae in water
Eggs on land, larvae in water
Direct development
Parental care
Internal development of tadpoles
Viviparity (at least 9 species)
Mostly external fertilization, however there are several independent origins of internal fertilization
Reptiles
Defining Characteristics
Elongate, armored bodies with osteoderms, long snout and relatively broad laterally compressed tails
Akinetic, flat skulls, modified diapsid type
Thecodont dentition – tooth has root imbedded in jaw (like us)
Legs relatively short with webbed feet
Aquatic
Eye with nictitating membrane
Lung ventilation via hepatic piston pump
multi cameral lung- multiple chambers
Four chambered heart
Lingual salt glands- except American alligator
All species oviparous with maternal care of eggs
Lack urinary bladder
Vertical cloaca
Egg tooth caruncle
Dome pressure receptors
Geographic Distribution
Alligatoridae
Southeast NA
Central and SA
Small part of eastern China (Alligator sinensis)
Crocodylidae
Central America and very top of SA
Everywhere but the Sahara and South Africa, in Africa
India, Indonesia and Malaysia and top of Australia
Gavialidae
Splotchy areas with water in India, Gavialis gangeticus
Malay Peninsula, Borneo and Sumatra, Tomistoma schlegelii
Mechanics of feeding
DEATH ROLLLLLLL
Diving adaptations
Submerged breathing
Closed throat, but internal naris allows for air to come through.
Cardiovascular
Cardiovascular adaptations
Blood flow can bypass lungs during a dive and a majority of blood is directed to the body (right to left shunt)
This happens because of vasoconstriction of lung blood vessels
A) crocodilians have divided ventricles
B) During steady air breathing, there is low pressure in right ventricle and high pressure in L. ventricle. Right ventricle has a valve that stays closed during this, it connects to the foramen of Panizza
C) During diving or breath-holding, both valves are open and both ventricles have same pressure. (Free flow)
Lung ventilation
Hepatic (liver) piston pump
The muscles attached to the pelvic girdle moves the liver back, increasing the volume in chest, causing negative pressure to suck air into the lungs
Reproduction
TSD
Complex parental care
Alligators and caimans generally construct above ground nests with vegetations, while crocodiles and Gahriels construct nests in soil
Young use vocalization to communicate with mother
Complex courtship behaviors as well
Bellowing
Audible bellow 'water dance'
Sub audible vibrations initiate pressure waves that are propagated through water
Head slaps, which can indicate location of male, sub audible vibrations may indicated size
Tool use
Mugger crocodile in stick displaying behavior, lure for birds
Similarity with birds
Territorial
Vocalization and communication
Nest construction
maternal care
Alligator mississippiensis
Range: South east USA, doesn't go further north than Virginia or south than Texas
Interesting Biological Traits
Thermoregulation: evaporative cooling, basking with their mouth open
Do alligators have determinant growth
Used growth rings on alligator long bones to determine growth rates
Determined that alligators and probably other crocodilians have determinant growth.
Generalist feeding strategy
Fish, turtles, reptiles, and mammals
Reproduction
Eggs are laid in a vegetation made mound by mothers, always in a dry area but near water
Salt water influx can destroy nests as freshwater is needed instead
Females use limbs and jaws to construct a nest mount
On average 3-5 ft in diameter, 2-3 feet in height
Up to 60 eggs deposited in each
Microbes eating vegetation and sun beating down heat the nest
Eggs are calcified and rough, ( sand paper)
When newly laid, they will form an opaque calcified band around the center of the egg if fertile
Up to 3 sires for each nest recorded
Nest attendance
Ecosystem engineers- animals that modify the environment in a way that is enhancing, they create wallows and little tracks and runways throughout the marsh
Some alligator mothers are better than others, will attend the nest consistently and leaving only occasionally to eat, drink or cool off. Others may leave more often, and this opens up an opportunity for nest predation.
Most predation occurs about 24-48 hours after being laid, so if they live that long, more chance of hatching.
Guard holes often dug by female during nest construction
Usually within sight of nest, connected by a run.
Defense of nest and young
Maternal female alligators are extremely defensive of their young and will defend their nest sites and their young
Hatchlings
Pattern fades as young grow older
Chirping/vocalization
Call for mother
Often from within nest mounds indicating for mother to open the nest
Egg tooth
How are they ecosystem engineers?
Create wet and dry conditions throughout the marsh
Holes
Trails
Nests
Their pathways allow for movement of other species, nests are used by many nest associates
Final Thoughts
Nuisance alligators are individuals that are at least 5 feet in length that pose a threat to pets, livestock, or humans
Nuisance alligators are created by humans feeding and/or harassing alligators
Alligators may be curious but they should not approach you
They have an innate fear of humans, once that fear is lost they pose a threat to both themselves and to you
Phylogenic position of Lepidosauria
Divulged later in time than turtles and crocs
Major Anatomical differences between Lepidosaurs and Archosaurs
Trait | Archosaurs | Lepidosaurs |
Antorbital Fenestra | Yes, secondarily lost in crocs | No antorbital fenestra |
Dentition | Thecodont | Pleurodont or acrodont |
Egg tooth | Caruncle (CaCO3 toothlike structure) | Fused to premaxilla |
Heart | 4 chambers (2 atria completely divided ventricle) | 3 chambers (single ventricle) some mixing of oxygenated and deoxygenated blood |
Cloaca | Longitudinal | Transverse |
Male Intromittent organ | Single | Eversion of cloaca in sphenodon, paired hemipenes in squamates |
Order Rhynchocephalia: Genus Sphenodon
Current distribution in New Zealand, normally on coastal islands, used to be on mainland but humans and their pets have pushed them through
Used to be found everywhere, but this is the only place found now
Efforts to reintroduced to the main island, surrounded by a wall to keep predators out to allow the population to grow.
Sphenodon punctatus and Sphenodon guntheri
Characteristics:
Unmodified diapsid skull
Complete jugal bar prevents free movement of quadrate
Premaxilla forms beak (much more in fossil forms)
Dentition acrodont, with premaxillary egg tooth present at hatching
One ro of teeth on btotom, two rows on top, not replaced if lost
Body generalized, limbs not specialized
23-35 trunk vertebrae
Transvers cloacal opening
No male intromittent organ, copulation by cloacal apposition as in birds
Ecology
Nocturnal, forages for insects at amazingly low body temp of 6-13 degrees Celsius
By day, shares burrows with shearwaters
Shear waters have a high Tb= 40 degrees Celsius, so provide warmth to sphenodon sharing burrows, KLEPTOTHERMY
Reproduction: oviparous w/ TSD, 6-10 eggs deposited in nests in the soil during autumn and overwinter in nests Hatchlings emerge in spring
Possible advantage of fall oviposition & spring hatchlings is that offspring emerge at a time of abundant recourses (dipause)
Major Subgroupings
Ascalobota | Autarchoglossa |
Iguanids | Scincids |
Chameleonids | Teiids |
Geckkonidds | Lacertids |
Pygopodids | Anguids |
| Varanids-monitor lizards |
Xantusiids (night lizards) may be transitional between ascalobotan and autarchoglossan
Division is based on ecological and anatomical/structural characteristics
Anatomical
Rectus superficialis muscle: absent in ascalobotans but present in autarchoglossan (except Xantusiidae)
Osteoderms- common in autarchoglossa, rare or absent in ascalobota
Scale rows/scale shape and margins
Ascalabota: Two 'cats' of scale type Autarchoglossan
Surface of hemipenes
Tongue: Necessary for food manipulation and drinking, but also for detection of non-volatile chemical compounds
DIAGRAMS:
Ascalobota Autarchoglossan
Used primarily for drinking, tasting,
And food manipulation and secondary olfaction
Ridges on tongue aid in food
manipulation
Ecological and behavioral differences
Ascalobotan
Visually oriented
Sexual dimorphism (color, sometimes size)
May have bright color
Ambush predator
No reduction of limb or toes
Autarchoglossan
Chemoreception (tend to be active foragers)
Color no, size yes
Cryptic or fossorial
Smell
Reduction of limb/toes common
Skull Morphology: Cranial Kinesis
Lizards show skull reduction and other modifications for three basic reasons:
Swallowing large prey
Compression
Burrowing
Skull morphologies
Typical: Unspecialized (Generalist diet)
Highly Kinetic:
Solid, akinetic:
Burrowing:
Archless: gekkotan
Reduced or fused arches heavy osteoderms:
Fossoriality and Limb loss
Most highly adapted fossorial lizards are autarchoglossans.
Limb loss seems to happen in the same way in every group:
Overall reduction in limb size
Lose components of hand
Lose components of forearm
Reduction of pelvic girdle and pectoral girdle: Note: pelvic girdle may be entirely missing but pectoral girdle has most elements remaining but reduced in size.
Defining features of Serpentes
No pectoral girdle
Features of the eye
Reduced left lung
Elongate body form
Unique skull characteristics
Parietal bone and later wall of skull reduced or absent
Highly mobile jaw elements (most)
Snake evolution
Historical predicted ecological associations:
Aquatic, subterranean, dense grasslands
Most likely ecological associations
Evolved from aquatic, possibly marine ancestors, burrowed in soft muddy environments
Ex: Lanthanotus borneensis (Lanthanotidae). Primitive lizard, likely an intermediate between helodermatids and varanids.
Broad taxonomic categories
Infraorder Scolecophida
Infraorder Henophidia
Infraorder Caenophidia
The Ophidian eye
Snakes: No ciliary body, no ciliary muscles, no fovea, no conus, no scleral ossicles. Eye lens yellow, color from oil droplets
Lizards: yellow pigmentation in photoreceptors
Method of accommodation
Snakes: contraction of iris muscles moves lens forward
Lizards: ciliary muscle acting on ciliary process compresses lens, alters curvature, similar to that of other terrestrial vertebrates including mammals
Families
Anomalepididae
Gerrhopilidae
Leptotyphlopidae
Typhlopidae
Xenotyphlopidae
All fossorial, active burrowers, or use tunnel systems of ants and termites on whose larvae and pupae they feed.
Taxonomy
Monophyletic group based on highly conserved morphological and ecological characteristics. Current taxonomy recognized five families
Recent molecular analyses suggest that the family Anomalepididae should not be included with Scoelcophidia. If so, Anomalepididae is either the sister lineage to all snakes (Scolecophidia and Alethinophidia or the sister lineage to Alethinophidia only. Either way these data suggest that the common ancestor of all snakes had a terrestrial scolecophidian phenotype.
Defining Characteristics
Small, 10 to no more than 95 cm in length
Smooth scales, lack enlarged ventral scutes, typical of most snakes
Tail short, often tipped with small spine, head blunt with reduced eyes
Retain elements of pelvic girdle
Skull highly modified for burrowing
Limited gape, teeth modified for slashing exoskeleton of soft-bodied prey
Left oviduct may be reduced or absent in some taxa
Species diversity
Anomalepididae: Central and South America on the coasts
Leptotyphlopidae: North, Central, and South America, Africa and some parts of the middle east
Rena humilis: Southern areas of California and Arizona
Gerrhopilidae: Indonesia and Malaysia
Xenotyphlopidae: Madagascar
Typhlopidae: Central and South America, Africa and Mediterranean, Southeast Asia and Australia (Gondwana)
Feeding
Specialize on soft-bodied prey (larvae, pupae, or eggs) sometimes consume adult termites or ants
Often gorge themselves when feeding, unlike most snakes which consume one or a few prey items at each feeding.
Some species spread cloacal secretions on body that mimics pheromones of ants or termites
Can push scales up, thrash, secret a smell from skin glands to ward off predators
Skull Morphology
Skulls generally adapted for consuming ant larvae, termite larvae and other soft-bodied prey via mandibular "raking". One species (Acutotyphlops subocularis) feeds on earth worms.
Families
Acrochordidae
Colubridae
Elapidae
Viperidae
Atractaspididae
Acrochordidae
Acrochordus arafurae - File Snake
Characteristics
Extremely large quadrate
Approx. 5% females become pregnant each year, 30 neonates per litter
Can reach high population densities
Seems to have a mix of caenophidian and henophidian characteristics
Ecological refugees, found in SE Asia and Northern Austrailia
Unique gape and suction feeding
Low mas-specific metabolic rate
Viviparous
Maxilla changes with venom delivery
Ligament connects the bottom jaws, therefore they can move independently
Feeding
Neck and body muscles used to push itself around prey. No ability to pull prey inside
Sequence: Upper jaw forward, then lower jaw in "walking motion" over prey
Teeth curved, helps to anchor jaw in place while eating
Copious amounts of saliva produced
JAWS DO NOT UNHINGE
Evolution of venom delivery
Duvernoy's gland: present in 30-40% of colubrid snakes
Produces mucopolysaccharides
Digestive enzymes
Amines
Antibiotic Function
Opens into ducts near back of enlarged maxillary teeth
Many if not majority of "Harmless" colubrid snakes are venomous as far as prey is concerned
Likely tremendous advantages to poisoning prey items
Phylogeny
Venom Delivery Systems
Venom
Elapids: Neurotoxic (amines, pre and post synaptic blocking agents
Viperidae: Fewer neurotoxic components, increase lysozymes
Elapids: Specialize for feeding on ectotherms
Viperidae: Specialize for feeding on endotherms
There are exceptions to both these generalizations
Infra-red photoreception
Rear Fanged Snakes
Ophisthoglyphous
Family Colubridae
Fixed or limited mobility, fangs : proteroglyphous fangs are fixed in place, relatively short
Derived condition found in mambas , where fangs can fold (limited) on roof of mouth (elapidae)
Folding fangs(solenglyphous) long, retractable fangs
Laterally deployed fangs, they are sythe shaped
Data
Approximately 8000 venomous snake bites each year in the US
On average mortality rate > 10 individuals per year
Of fatal bites, 95% are due to rattlesnakes
In the American South, most common snake is from the copperhead (Agkistrodon contortrix)
North Carolina is the state incidence of snakebite
Bites from coral snakes (Micruroides and Micrurus)
Symptoms
Pit Viper
Elapid
Hemotoxic (generally)
Neurotoxic
Intense pain
Minimal Pain
Edema
Pstosis (droopy eyelid)
Numbness, tingling
Paresthesia
Rapid Pulse
Diplopia
Muscle twitching
sweating
paresthesia
Sweating
Metallic taste
salivation
vomiting
hyporeflexia
confusion
Respiratory depression
Bleeding disorders
paralysis
If untreated, or in severe cases bite may lead to intravascular coagulation, renal failure and or hypovolemic shock
If untreated, or in severe cases, envenomation may lead to respiratory paralysis and death
Emergency treatment best practices:
For bites of pit-vipers
DO NOT add tourniquet or other device to restrict blood flow
DO NOT "cut and suck" venom from bite site
DO immobilize bitten extremity
DO keep victim calm and avoid activity
DO transport to hospital ASAP
Often snake bites are "dry", that is, no venom is injected. For this reason, snake bites should be evaluated by medically-trained personnel before any invasive treatments are given
Medical Treatment
CroFab (Crotalidae Polyvalent Immune Fab) anti-venom is the current treatment for all North American pit-vipers.
Anti-pitviper antibodies are generated in sheep. Antibodies generated in sheep result in reduced probability of allergic response compared to anti-sera generated from horse.
Multiple vials (3-4) typically required for treatment at a total cost of about $4,000 to $5,000 per vial
Anti-inflammatory drugs (epinephrine and anti-histamines) also given
Characteristics
Fossorial diapsid reptiles
Skull: Jugal, post-orbital and squamosal bones absent
Strong jaws, large teeth
Heterodont teeth: Extremely unusual for reptiles
Grouped with lepidosaurs on basis of sharing
Transverse cloacal aperture
Paired hemipenes
Premaxillary egg tooth
Acrodont or pleurodont dentition
Differ from rhynchocephalians and squamates in:
Akinetic skull
Left lung functional (unlike snakes)
Skull with a marked craniofacial angle
Frontal bone forms lateral wall of brain case
Middle ear apparatus modified by association of epihyal cartilage and stapes
Teeth reduced in number but large in size
Pseudocopulation: false copulation behavior that is known to occur in Aspidoscelis to allow for ovulation for parthenogenesis.
Distribution of parthenogenesis in squamates
Relatively wide occurrence in lizards and less commonly in snakes
Best studied group are whiptail lizards (genus Aspidoscelis): At least 17/45 species are parthenogenesis
Old world genus Lacerta 30/60 parthenogenic species
Also some geckos, xantusiids, iquanids, varanids, chameleons, agamids, and a few snakes including pit vipers
Ramphotyhlops braminus (Flower pot snake): all specimens found so far are female
Mechanism
Where genetic mechanisms are known, parthenogenesis results from extra premeiotic endoduplication of chromosomes followed by normal meiosis
Result: egg with two maternal chromosome compliments initiating development without fertilization
Sometimes resulting child can mate with a male of either parental species, then you get a triploid
How hybridize?
Origin of hybridization seems to be associated with habitat disturbance including anthropogenically induced habitat destruction.
Some origins of viviparity are ancient (e.g. boas). Age makes it difficult to determine the selective cause of viviparity in these groups
Lizards have more recent origins of viviparity, so we can test hypotheses as to what selective agents, cause viviparous reproduction
Reptiles and amphibians are in decline worldwide
Habitat loss/Alteration
Climate change temperature, moisture (rainfall patterns), sea level rise
Poaching, collecting
Disease:
Amphibians: Chytrid Fungus- can be an important source of mortality in some populations
Snakes: Ophidiomyceces- produces/results in snake fungal disease