Neuron Communication and Plasticity

Chapter 3 Part II: Neuron Communication and Plasticity
How Do Neurons Communicate and Adapt?
  • Neurons (nerve cells) talk to each other and change over time. This section covers:

    • How they send chemical messages.

    • The different kinds of neurotransmitters (chemical messengers) and the receptors (receiving stations) they use.

    • How these neurotransmitter systems affect our behavior.

    • How synapses (connection points) adapt, which is crucial for learning and memory.

A Chemical Message
  • Otto Loewi (1921): He was the first to show that neurons communicate using chemicals. He did an experiment with frog hearts.

  • Vagus nerve and acetylcholine (ACh): Loewi found that stimulating the vagus nerve released a chemical, later identified as ACh, that slowed the frog's heart rate.

    • Acetylcholine (ACh): This was the first chemical messenger found in both the brain (CNS) and the rest of the body (PNS).

      • In the somatic nervous system (SNS), which controls voluntary movements, ACh makes skeletal muscles contract.

      • In the autonomic nervous system (ANS), which controls involuntary body functions, ACh can either speed up or slow down internal organs.

The Basis of Neural Communication in a Heartbeat
  • Our heart rate changes to match our body's needs for energy, food, and oxygen.

  • Loewi's experiment showed that a chemical from the vagus nerve told the heart to slow down.

  • He later found two types of chemical messages involved in heart rate:

    • Excitatory message: Makes the heart beat faster.

    • Inhibitory message: Makes the heart beat slower.

How Does a Neuron Pass on a Message?

Procedure

  1. Stimulating device: The vagus nerve of a frog's heart (heart 1) is given an electrical signal.

  2. Fluid transfer: The fluid around heart 1 (which now contains the chemical messenger) is moved to a second container with another frog heart (heart 2).

  3. Recording device: Heart 1's beating slows down after the nerve stimulation. Interestingly, heart 2 also slows down after receiving the fluid from heart 1, proving a chemical message was transferred.

Loewi’s Complementary Experiments
  • Otto Loewi also studied other important chemical messengers:

    • Epinephrine (EP) (also known as adrenaline): This works as a hormone (a chemical messenger in the bloodstream) to prepare the body for "fight or flight" during stress. It also acts as a neurotransmitter in the brain.

    • Norepinephrine (NE) (also known as noradrenaline): This acts as a neurotransmitter in the brain and in the part of the nervous system that calms the body (parasympathetic ANS). It speeds up heart rate in mammals.

Neurotransmitters
  • Neurotransmitter: A specific chemical released by one neuron (the sender) that travels to another target cell (the receiver), causing either an excitatory (activating) or inhibitory (slowing down) effect.

  • Many neurotransmitters found in the brain also act as hormones when they are in the bloodstream outside the brain. Hormones usually have slower and longer-lasting effects than neurotransmitters.

  • Loewi's work opened the door for scientists to find more neurotransmitters.

  • We don't know the exact number, but there are about 60 confirmed neurotransmitters and around 200 possible ones.

Structure of Synapses
  • With the invention of the electron microscope in the 1950s, scientists could see the tiny structures where neurons communicate.

    • They found that neurotransmitters are stored in small sacs called vesicles at the end of the axon (the sending part of the neuron).

  • Chemical synapse: This is the tiny gap or junction where chemical messengers (neurotransmitters) are sent from one neuron to either excite or inhibit the next neuron.

    • Most communication points in the mammalian brain are chemical synapses.

Chemical Synapse Depicted
  • Imagine two neurons talking, with a small gap between them.

    • Presynaptic terminal: This is the end of the sending neuron's axon. It contains the chemicals (neurotransmitters) ready to be released. "Pre" means before the synapse.

    • Synaptic cleft: This is the very small space between the sending (presynaptic) neuron and the receiving (postsynaptic) neuron.

    • Postsynaptic membrane: This is the surface of the receiving neuron (often on a dendrite or cell body). It has special receptor molecules that catch the chemical messages. "Post" means after the synapse.

    • Synaptic vesicle: These are small, round sacs inside the presynaptic terminal that hold the neurotransmitters.

    • Storage granule: These are larger compartments that hold many synaptic vesicles.

    • Dendritic spine: These are small bumps on the dendrites of the receiving neuron where most neurotransmitter signals are received.

Parkinson Disease
  • More than 50 years after James Parkinson first described the symptoms, French neurologist Jean-Martin Charcot named it Parkinson's disease.

  • What causes it in the brain:

    • There is a loss of brain cells in a specific area called the substantia nigra.

    • Symptoms appear when the levels of dopamine (DA), a key neurotransmitter, drop below 10% of normal in a brain region called the basal ganglia.

    • Studies in rats showed that destroying dopamine-producing neurons caused symptoms similar to Parkinson's disease, confirming that dopamine's pathway from the substantia nigra to the basal ganglia is crucial for movement control.

Terms to Learn: Structure of Chemical Synapses
  • Presynaptic membrane (the outer skin of the axon terminal, where chemicals are released)

  • Postsynaptic membrane (the outer skin of the dendritic spine, where chemicals are received)

  • Synaptic cleft (the tiny gap in between)

  • Tripartite synapse (a concept that includes the presynaptic neuron, postsynaptic neuron, and nearby glial cells, all working together at the synapse)

  • Synaptic vesicle (sacs holding neurotransmitters in the presynaptic terminal)

  • Storage granule (larger containers for vesicles)

  • Postsynaptic receptor (special proteins on the postsynaptic membrane that neurotransmitters bind to)

  • Anterograde synaptic transmission (the usual direction of message flow: from presynaptic to postsynaptic neuron)

  • Transporter (proteins that move molecules, like neurotransmitter precursors into the neuron or neurotransmitters back into the neuron)

Neurotransmission in Five Steps

This is how neurons send chemical messages:

  1. Synthesis: Neurotransmitters are made from simpler building blocks (precursor molecules).

  2. Packaging and storage: The newly made neurotransmitters are put into vesicles and stored, waiting for an electrical signal (action potential) to arrive.

  3. Release: When an action potential (the electrical signal) reaches the axon terminal, it opens special calcium (Ca2+Ca^{2+}) channels. Calcium ions rush in, causing the vesicles to merge with the cell membrane and release their neurotransmitters into the synaptic cleft. This process is called exocytosis.

  4. Receptor action: The released neurotransmitter floats across the synaptic cleft and binds to specific receptors on the postsynaptic neuron's membrane. This binding changes the postsynaptic neuron.

  5. Inactivation: The neurotransmitter's job is done, so it's removed from the synaptic cleft in one of several ways.

Step 1 and 2: Neurotransmitter Synthesis, Packaging, and Storage
  • Where neurotransmitters come from:

    • Many are made right in the axon terminal from ingredients we get from our diet. Special proteins called transporters help bring these building blocks into the cell.

    • Some, especially larger protein-based ones called peptide transmitters, are made in the neuron's main cell body (following DNA instructions) and then moved down the axon to the terminal using microtubules (tiny cellular roads).

  • Types of neurotransmitter basic structures:

    • Peptide (protein-like)

    • Lipid (fat-like)

    • Gaseous (like nitric oxide)

    • Ion (like zinc ions)

Step 3: Neurotransmitter Release
  • When the electrical signal (action potential) arrives at the axon terminal, it causes special gates called voltage-sensitive Ca2+Ca^{2+} channels to open.

  • Calcium (Ca2+Ca^{2+}) ions then rush into the cell. These calcium ions bind to a protein called calmodulin, creating a complex that signals the synaptic vesicles to fuse with the presynaptic membrane and release their neurotransmitters into the synapse via exocytosis.

Step 4: Receptor-Site Activation
  • Once released, the neurotransmitter crosses the synaptic cleft and attaches to specific proteins called receptors on the postsynaptic membrane:

    • Transmitter-activated receptors come in a few forms:

      • Ionotropic receptor: These are like fast-acting gates. When a neurotransmitter binds, an ion channel immediately opens, allowing ions (charged particles) to flow into or out of the cell, changing its electrical state quickly.

      • Metabotropic receptor: These are slower. When a neurotransmitter binds, it starts a chain reaction inside the cell, which can lead to various internal cellular changes, including eventually opening ion channels, but it takes more steps and is therefore slower.

      • Autoreceptor: These are special receptors located on the presynaptic neuron itself. They monitor how much neurotransmitter is being released and can regulate (either increase or decrease) further release.

    • Quantum (plural: quanta): Neurotransmitters are released in discrete, small packets, not continuously. Each packet is called a quantum.

Step 5: Neurotransmitter Inactivation
  • After stimulating the receptor, the neurotransmitter must be removed from the synaptic cleft to stop its effect and prepare the synapse for new messages. This can happen by:

    • Diffusion: The neurotransmitter simply floats away from the synaptic region.

    • Degradation: Enzymes (biological catalysts) break down the neurotransmitter into inactive parts.

    • Reuptake: The presynaptic neuron reabsorbs the neurotransmitter back into its terminal, often to be reused. Special transporter proteins on the presynaptic membrane perform this task.

    • Astrocyte uptake: Glial cells called astrocytes, which are support cells in the brain, can also absorb neurotransmitters from the synapse.

The Versatile Synapse
  • Synapses are incredibly diverse throughout the nervous system. They differ in their location, structure, how they work, and what cells they connect to.

  • This variety allows the synapse to act as a highly flexible chemical delivery system, fine-tuning how neurons communicate by connecting to different parts of the receiving neuron (dendrites, cell body, or axon).

Electrical Synapses
  • While most synapses are chemical, some are electrical synapses.

    • Gap junctions: These are direct connections between neurons, formed by channels made of proteins called connexins. Ions can flow straight from one neuron to the next through these junctions.

    • They act like regulated gates, opening or closing, and allow for very fast communication because there's no delay from chemical diffusion.

    • Electrical synapses also help glial cells and neurons communicate and allow for "mixed synapses" where both chemical and electrical signals are used.

Excitatory and Inhibitory Messages
  • Synapses can be specialized to either excite or inhibit the receiving neuron:

    • Excitatory synapse: These usually make the receiving neuron more likely to fire an electrical signal.

      • They are typically found on dendrites.

      • They have round vesicles, dense material on their membranes, a wider synaptic cleft, and a larger active zone (where neurotransmitters are released).

    • Inhibitory synapse: These usually make the receiving neuron less likely to fire an electrical signal.

      • They are generally found on the cell body.

      • They have flat vesicles, less dense material on their membranes, a narrower synaptic cleft, and a smaller active zone.

Dendritic Spines: Small but Mighty
  • Dendritic spines: These tiny bumps on dendrites are extremely important for learning and memory.

    • Each spine acts almost independently and can change its shape and size, making it a key part of how the brain learns and stores information.

    • They form the physical basis for behaviors, skills, and memories.

    • Problems with spine formation are linked to certain mental disabilities, and the loss of spines is seen in diseases like Alzheimer's.

Evolution of Complex Neurotransmission Systems
  • The idea of chemical communication might have evolved from very basic processes, like how single-celled organisms feed.

    • For example, secreting digestive juices (exocytosis) is similar to how neurotransmitters are released.

    • Capturing food (endocytosis) is similar to how neurons receive signals. This suggests a deep evolutionary root for current neurotransmission.

Classes of Neurotransmitters
  • Neurotransmitters are grouped based on their chemical makeup:

    • Small-molecule transmitters

    • Peptide transmitters (chains of amino acids)

    • Lipid transmitters (fat-based)

    • Gaseous transmitters (gas molecules)

    • Ion transmitters (ions)

Small-Molecule Transmitters
  • These are fast-acting neurotransmitters made in the axon terminal from nutrients in our diet. They are quickly packaged and ready for use.

    • Acetylcholine (ACh): Involved in muscle contraction and memory.

    • Amines:

      • Dopamine (DA): Important for reward, motivation, and motor control (lack of DA causes Parkinson's).

      • Norepinephrine (NE) (noradrenaline): Involved in alertness, arousal, and mood.

      • Epinephrine (EP) (adrenaline): Works as both a hormone (stress response) and a neurotransmitter.

      • Serotonin (5-HT): Affects mood, sleep, appetite, and learning.

    • Amino acids: These are the building blocks of proteins and also act as neurotransmitters.

      • Glutamate (Glu): The main excitatory neurotransmitter in the brain, crucial for learning and memory.

      • Gamma-aminobutyric acid (GABA): The main inhibitory neurotransmitter in the brain, helps calm brain activity.

      • Glycine (Gly): Another inhibitory neurotransmitter, mainly in the spinal cord.

      • Histamine (H): Involved in wakefulness and immune responses.

    • Purines:

      • Adenosine: Can act as an inhibitory neurotransmitter, affecting sleep and reducing