Sleep and Arousal

  • Sleep

    • Circadian Rhythms

    • Suprachiasmatic nucleus

      • "blocking clock"

      • Light stimulates the SCN and results in decreased melatonin production by pineal gland in the morning

      • Low light (darkness) leads to increased melatonin production by pineal gland

    • Chronotype

      • Alters with age

      • Young

        • Night owls

          • Performance is worst in the morning

      • Older

        • Morning larks

          • Performance is best in the morning

    • Why do we sleep?

      • Recuperation Theories of Sleep

        • Restorative Theory

          • Repair and regeneration of the body?

          • Muscle repair, tissue growth, protein synesis, growth hormone release

          • Necessary for optimal functioning of physiological systems

        • Elimination Theory

          • Rid the brain of excess sensory information?

          • Certain synaptic connections are strengthened, and others are weakened (or not established) during sleep

          • Synaptic pruning

        • Brain Plasticity Theory

          • Neural reorganization, growth of neurons, and brain structures

      • Adaptation Theories of Sleep

        • Immobilization Theory

          • Early theory of sleep

          • Why we sleep is to encourage us to be less active where we are most vulnerable

          • What prompts early humans to have about 8 hours of sleep?

            • Why do we sleep during the dark part of the cycle?

              • We can't see in the dark

              • Predators

              • Invasions

              • Ability to navigate is weak

              • Increased risk of injury

          • Innate response with species specific patterns

          • Keeps one inactive and safe during least efficient part of day/nigh cycle

        • Energy Conservation Theory

          • We can survive by having a period of time where we are conserving energy

          • Following survival activities, periods of inactivity are a good way to conserve energy

            • Such as, hunting, gathering, food preparation, and eating

          • About 1/3 of the day or our life is spent sleep

            • Time to digest the food we eat

          • Metabolism rate decreases about 10% during sleep

            • Burning less energy during sleep

      • Functions of Slow-Wave Sleep

        • Deeper state of sleep that seems to be when our recuperation and recovery takes place

        • 50-70% of growth hormone is released

        • Seems essential for survival

          • E.g., Fatal familial insomnia

            • Hereditary disease

            • Protein in the brain start to unfold in unseal ways and disrupts activity in part of the brain

              • Thalamus

                • Malfunctions

            • Slow wave sleep stops first

            • 6 months within them not sleeping they die

        • Lack of SWS correlated with various disorders

          • Cardiovascular

          • Diabetes

          • Obesity

          • Alzheimer's

        • Consolidation of declarative memory

          • Explicit memory

          • Allows you to talk about your experiences

            • Dates

            • Times

            • Information you can talk about and review

            • Information you can describe 

      • Functions of REM Sleep

        • Dreaming

        • Promote brain development

        • Facilitate learning

        • Consolidation of nondeclarative memory

          • Implicit memory

            • Things we can't talk about

            • More about remembering how to do particular tasks

              • Tying your shoe

              • Riding a bike

            • More important for learning tasks

        • REM rebound phenomenon

          • Indicated biologically than REM sleep is better than Slow-Wave sleep

            • If you are sleep deprived, you will have more REM sleep

              • Make up REM first and then Slow-Wave

      • True or False?

        • "Driving sleepy is just as bad as driving drunk"

          • "Therefore, after 17 hours of sustained wakefulness cognitive psychomotor

performance decreased to a level equivalent to the performance impairment

observed at a blood alcohol concentration of 0.05%. This is the proscribed

level of alcohol intoxication in many western industrialized countries. After 24

hours of sustained wakefulness cognitive psychomotor performance

decreased to a level equivalent to the performance deficit observed at a blood

alcohol concentration of roughly 0.10%.”

  • Dawson, D., & Reid, K. (1997). Fatigue, alcohol and performance impairment. Nature,

388(6639), 235-235

  • REM Sleep and Development

    • Infants and Toddlers sleep a lot

      • Brain develops faster

      • Dream more

    • The older we get the less sleep we get

      • Brain growth and development slows down

      • Cognitive decline in elders

      • Dream less

  • Species Specific Hours of Sleep per Day

    • Large differences found between species

      • Bat

        • 19.9 ( a lot)

      • Horse

        • 2.9 (little)

    • Metabolic rate

  • Measurement of Arousal and Sleep

    • Physiological Measures of Arousal and Sleep

      • Electroencephalogram (EEG)

        • Electrical potential recorded from electrodes placed on the scalp

          • "Brain Waves"

            • Swimmers cap

        • Looks at patterns where particular waves show wakefulness and levels of wakefulness

        • Different stages of sleep have different patterns of waves

      • Electrooculogram (EOG)

        • Measure of eye movements seen during sleep

        • Electrodes placed on eyebrow, below eye, and on each side

        • SWS

          • Slow rolling eye movements

            • Think: Eyeball floating in water 

        • REM

          • Rapid eye movements

            • Left-right (fast)

      • Electromyogram (EMG)

        • Electrical potential recorded from an electrode placed on muscle

        • Measures changes in muscle tension particularly facial and neck muscles

          • Mentalis muscle

            • Muscle on chin

              • Connects lower lip to chin

            • Muscle completely relaxes when asleep

          • Other muscles still have tone

  • Theories of Sleep

    • Passive Theory of Sleep

      • Bremer (1936)

      • Cerveau isole

        • Isolating the forebrain

        • Continuous state of slow-wave sleep

        • Strong odor could wake then up and within minutes fell back asleep

      • Encephale isole

        • Cut the brain from the spinal cord

        • Normal sleep-wave cycle

        • Ignored data

          • Focused more on isolating the forebrain

    • Active Theory of Sleep

      • Moruzzi and Magoun (1949)

      • Discovered the reticular formation

    • Four Pieces of Evidence that the Reticular Activating System is Involved in Sleep

      • (2) only cutting the small section of reticular

        • When they cut just reticular information, the tasks show the exact same slow-wave pattern as if the cut the whole brain

        • Bremer is WRONG

  • Sleep Characteristics and Neurotransmitters

    • Sleep-Wake Cycle

      • More through sleep in particular order

        • Stage 1 sleep

          • Only occurs when we are coming out of wakefulness (falling asleep)

          • Roughly, 1-7 minutes before descending into stage 2 

        • Stage 2 sleep

          • You feel like you have been asleep, but you also feel your are right on the edge

          • Roughly, 10-20 minutes

          • About 50% of total sleep time

          • Gets longer towards later cycles

        • Slow-Wave sleep

          • Deepest sleep

          • Roughly, 20-40 minutes

          • Difficulty waking up

            • One thing that can wake people up is calling their name

          • Nightmares tend to occur

          • Sleepwalking can occur

          • More time in stage 3 earlier than later

        • Stage 2 sleep

        • REM sleep

          • Dream stage

          • Roughly, 20-25% of total sleep time

          • Early in the night is shorter

      • On average, 1-1.5 hours per cycle

    • Sleep Stages and Brain Waves

      • Gamma Wave

        • Irregular

        • Low amplitude

        • Highest frequency

          • About 30 - 120 Hz

        • Hyper focused

      • Beta Wave

        • Irregular

        • Low amplitude

        • High frequency

          • About 13- 30 Hz

        • Thinking and processing

      • Alpha Wave

        • Fairly regular

        • Low amplitude

        • High frequency

          • About 8 - 13 Hz

        • Occur when we are relaxed

      • Theta Wave

        • Low amplitude

        • Moderate frequency

          • About 4 - 8 Hz

        • Indicator of falling asleep

      • Sleep Spindles

        • Short bursts

          • About 12 - 14 Hz

        • Extremely short

        • Correlated with noises

      • K Complexes

        • Sudden sharp waveforms

        • Tactile stimulation

      • Delta Wave

        • High amplitude

        • Low frequency

          • About 1 - 4 Hz

      • Activation synesis 

    • Arousal and Neurotransmitters

      • Acetylcholine

        • Dorsal pons and basal forebrain

        • Plays role in arousal of cerebral cortex

        • Levels high during wakefulness and REM

        • Project to

          • Medial pons, thalamus, cortex

        • Involved in cortex and hippocampus arousal

      • Norepinephrine

        • Locus coeruleus (in pons)

        • Plays roles in attention and vigilance

        • Possible role in "behavioral" arousal

        • Levels high only during wakefulness

          • Lower levels during SWS and lowest during REM

        • Project and impact

          • Cortex, thalamus, hippocampus, cerebellum, pons, and medulla

      • Serotonin (5-HT)

        • Raphe nuclei

          • Medial pons

            • Near caudal end of reticular formation

        • Levels high during wakefulness

        • Levels lowering as descending towards REM

        • Cortical and behavioral arousal

        • Plays role in activating behavior

          • Pacing, chewing, grooming in rodents

        • Projects to and impact

          • Thalamus, hypothalamus, cortex, hippocampus, basal ganglia

      • Histamine

        • Tuberomammillary nucleus

          • In hypothalamus

        • Levels high during wakefulness

        • Levels low during SWS and REM

        • Implicated in control of wakefulness and arousal

          • i.e., an antihistamine is Benadryl

        • Project to and impact

          • Cortex, thalamus, hypothalamus, basal ganglia, basal forebrain

      • Orexin

        • From lateral hypothalamus

        • Levels high during wakefulness

        • Low during rest and all sleep stages

        • Increase activity in the brain stem and forebrain arousal systems

          • Has a stimulatory effect on all other areas of the brain talked about previously

    • Slow-Wave Sleep and Neurotransmitters

      • GABA (GABAergic neuron)

        • Major inhibitory neurotransmitter

        • Doesn't promote Slow-Wave sleep

          • Indirect role

        • From ventrolateral preoptic area (vlPOA)

          • Quite down and inhibit the arousal areas of the brain

        • Suppress alertness and behavioral arousal and promote sleep

        • Disruption to the brain stem, thalamus, hypothalamus, and cortex

      • Adenosine

        • Peptide released by neurons during high levels of metabolic activity through out the day

          • More active you are, more adenosine released

          • Highest when you fall asleep

        • Increases activity in the vlPOA

  • Brain Mechanisms

    • The Sleep/Waking Flip Flop

      • When the flip-flop is in the "wake" state, the arousal systems are active and the vlPOA in inhibited (a)

        • GABA is coming from the arousal areas and having an effect on vlPOA

          • Result is vlPOA is inhibited

        • When the flip-flop is in the "sleep" state, the vlPOA is active and the arousal systems are inhibited (b)

        • Solid line

          • Neurotransmitters are being released

        • vlPOA

          • Ventrolateral preoptic area

        • vlPAG

          • Ventrolateral periaqueductal gray

        • SLD

          • Sublaterodorsal nucleus

 

 

 

  • REM Sleep Brain Mechanisms

  • Narcolepsy

    • Symptoms of Narcolepsy

      • Sleep attack

      • Cataplexy

      • Sleep paralysis

      • Hallucinations

        • Hypnagogic

        • Hypnopompic

      • Additional symptoms

        • Difficulty staying awake during the day

        • Difficulty staying asleep during night

          • Fragmented sleep

        • REM sleep intrudes into waking state

        • Seem to skip SWS and enter REM sleep quickly

      • Prevalence

        • 1 in 2000

    • Causes of Narcolepsy

      • Seems to be related to deficiency of peptide neurotransmitter orexin

      • Mutation in orexin B receptor (causes canine narcolepsy)

      • In humans

        • Complete absence of orexin in 7 out of 9 persons with narcolepsy

      • Most are born with orexin, but during adolescence the immune system may attack these neurons and symptoms begin

    • Treatment of Narcolepsy

      • Stimulants

        • Ritalin and Amphetamine

          • Methylphenidate (Ritalin)

            • Dopamine and norepinephrine agonist

              • Reuptake inhibitor

          • Amphetamine

            • Dopamine & norepinephrine agonist

              • Reuptake inhibitor

          • Modafinil (Provigil)

            • Orexin agonist

            • Typically taken in the morning

              • Help to reduce daytime sleepiness

                • I.e., decrease sleep attacks

        • SSRIs and SNRIs

          • Fluoxetine

            • Prozac, Serafem, others - SSRI

          • Venlafaxine

            • Effexor - SSRI

          • Atomoxetine

            • Strattera - SNRI

          • Typically taken later in the day

            • Help to reduce episodes of REM sleep components

              • I.e., episodes of cataplexy, sleep paralysis, and hallucinations

        • Tricyclic Antidepressants

          • Norepinephrine, serotonin, dopamine agonist

            • Reuptake inhibitor

          • Protriptyline

            • Vivactil

          • Imipramine

            • Tofranil

          • Typically taken later in the day

            • Help to reduce episodes of REM sleep components

              • I.e., cataplexy, sleep paralysis, and hallucinations

        • Sodium Oxybate

          • Xyrem

          • CNS depressant that reduces excessive daytime sleepiness and cataplexy

          • GABA-B receptor agonist

          • Taken at night

            • Immediately before bed

            • Second dose 3-4 hours later

              • Middle of the night