Centrifugation Basics

Basics of Centrifugation in Biology

  • Definition: Centrifugation involves rotation around a single axis, creating a rotational force that moves outward.

  • Purpose: Used to gather samples together and separate particles within a single sample.

Types of Centrifuge Settings

  • Time Setting: E.g., 30 seconds (adjustable).

  • Speed Measurement Units:

    • RPM (Revolutions per Minute): Measures revolutions around the circle per minute.

    • RCF (Relative Centrifugal Force): Measures force relative to gravitational force (g).

Centrifugation Applications

  • Sample Collection:

    • After spinning, samples collect at the bottom, with no dispersion remaining.

    • Important for small volumes where droplets can affect results.

  • Balancing Samples:

    • Load samples evenly around the centrifuge to maintain balance.

    • For uneven weights, balance with equal volumes of water or similar.

  • Separating Particles:

    • Can separate different particle weights within a solution (e.g., bacteria).

    • Use of a pipette tip to carefully remove supernatant without disturbing the pellet.

  • Final Outcome: Precise pellet formation at the bottom of the tube, free from supernatants, ready for further experiments.