Recording

Pyrogen Testing

• Limulus Amebocyte Lysate (LAL) Test:

  • An in vitro assay derived from the horseshoe crab's blood.

  • It's based on clotting; if clotting occurs, it indicates the presence of pyrogens or endotoxins.

  • Advantages: Faster and cheaper than the alternative rabbit pyrogen test.

  • Both LAL and Rabbit Pyrogen tests are listed in industry guidelines for endotoxin detection.

• Rabbit Pyrogen Test:

  • An in vivo assay involving injecting a protein sample (potentially contaminated with endotoxins, also known as pyrogens) into the ear of a rabbit.

  • If the rabbit develops a fever, it indicates the presence of pyrogens.

  • Ethical Considerations: This method involves animal testing, and with the