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Systematic Biology (Plants)
LEC - 60%
Term/Final Exams – 30%
Quizzes – 20%
Journal Critique/Projects/Assignments – 10%
Plant Collection and Specimen Preparation
Fundamental aspects of study, training and research in plant systematics
Herbarium specimens are permanent records of plant species at a particular place and time.
Plant Collection
Collect entire, vigorously growing typical specimens.
Avoid insect-damaged specimens.
Collect underground parts (bulbs, rhizomes, tubers) of herbaceous perennials.
Collect specimens with flowers, fruits, and seeds.
For grasses, herbs, and small annuals, collect the whole plant including underground parts.
For trees and shrubs, obtain mature healthy branches with leaves, flowers, and fruits intact.
Collect three to four samples per plant/plant colony.
Assign a collection number for each plant.
Record information about the plant (locality, habitat, description, etc.) in a field notebook.
Specimens larger than a single sheet should be divided and pressed on a series of sheets.
Collect plants with leaves intact.
Collect bark and wood samples of woody plants.
Collect plant samples in the afternoon, except when rainy
Put tagged specimens in plastic bags, then drain them in alcohol before pressing.
Never collect the only plant of a species in a locality.
Field Equipment and Supplies
Field Press: Hardwood, metal or strong plywood frames of 12 by 18 inch
Driers or Blotters: Heavy blotting papers or moisture-absorbing materials of 11 by 16 inch
Straps or Ropes: Strong web straps of about 4-5 feet length to tighten the press
Corrugate Ventilators: Sheets of corrugated cardboard of 12 by 18 inch between driers; Provide space for air passage to remove moisture
Field Notebook: Used to record date, place, locality, habitat, elevation, local name, collector number.
Digging and Clipping Tools: Trowel, digger, hammer, pruning shears, garden clippers, geological pick, heavy sheath knife
String Tags: Waterproof material for labeling plants not immediately pressed
Vasculum: Container made of tin or aluminum to preserve freshness
Collecting Bags: Plastic bags for fresh specimens
Collection Bottles: Glass or plastic bottles with leakproof screw caps for small material in liquid preservatives
Liquid Preservatives: Formalin-acetic acid-alcohol (FAA) for anatomy materials; Chloroform for cytology materials
Hand Lens: 5x or 10x lens
Waxed Paper: For pressing viscid or weak plants
Cardboard Storage Boxes: To store dry materia;ls
Maps: For determining localities for particular species
Color Charts: Use in determining the actual color of the parts (flower, leaves) in the field itself
Camera: Taking of photographs in the field
Others: Compass, thermometer, seed envelopes, insect repellent, psychrometer
Organization of The Field Press
Hardwood or plywood press
Corrugate ventilator
Drier or blotter
10 sheets of torn newspapers (each sheet will contain one specimen)
How to Press Plant Specimens?
Press specimens as soon as possible
Place specimen carefully on a pressing sheet
Remove extra leaves or branches if necessary
Plants too large may be bent into a V, N, or M figure. Specimens should not protrude from the fold of the paper
Expose some upper and lower surfaces of leaves
Spread out flowers or inflorescence thoroughly
A few flowers can be cut longitudinally and pressed, if possible
Each sheet should contain a collector’s number which refers to the notes in the field notebook
Place specimens in between driers or blotters
Place all specimens for pressing so that the bundle is of uniform thickness
Bind the press tightly with ropes or straps to prevent wrinkling
Drying of Specimens
Dry specimens as rapidly as possible.
Place the press in the sun
After 24 hours, open the press and place specimens in fresh blotters.
Rearrange plant parts at this time
Bind the press tightly again with fresh blotters
Dry wet blotters in the sun for reuse
Change wet blotters or driers daily for 3-4 days, until specimens are completely dried
The press becomes loose when the plants are completely dried
Artificial heat may be used for drying, but never use an oven
In humid regions, use a drier
Drier
Wooden box (3 ft in length and 18 inches in breadth made up of 1/10 inch thick boards)
Five light bulbs of 60 watts are fitted inside for heat
Small openings are made of the bottom for air entry
A press containing 100 specimens can be dried in 8-12 hours
Mounting of Specimens
Attach a dry specimen to a mounting paper or herbarium sheet, and affix a label at the lower right corner.
Herbarium sheet size: 28.75 x 41.25 cm (11.5 x 16.5 inch)
Before mounting, dip dry specimens in saturated solution of mercuric chloride in ethyl alcohol to prevent infection.
Poisoning
12.15 grams mercuric chloride, 5.10 grams of phenol crystal, 1.0 liter of denatured alcohol (CAUTION: HIGHLY CORROSIVE AND POISONOUS CHEMICALS)
Use only denatured alcohol or lauryl pentachlorophenate
Mounting of Specimens
Apply glue or paste (Elmer’s glue) to the back of the specimens
Place loose parts (seeds, fruits) in a paper packet and paste on the herbarium sheet
Labeling of Specimens
Size: 6.5 X 10.5 cm, pasted on the lower right side of a herbarium sheet
High rag quality paper used
Information on labels:
A. Heading – institution
B. Family name
C. Local or vernacular name
D. Scientific name – genus, species along with authority
E. Locality – place of collection
F. Habitat – vegetation type
G. Collector
H. Determined by – the one who authenticated the identification
I. Collection number – starting with 1 and continuing throughout the lifetime of the collector
J. Additional Information (color of leaves & flowers, associated plants, economic uses etc.)
Labels
Field Label: Upper left hand corner, includes common/vernacular name; scientific name; date; locality; habitat; important plant notes
Herbarium Label: Lower right hand corner, includes herbarium no., family name, scientific name, collector, determined by, collector number
Fluid Preservation
Fleshy fruits, large flowers, and other botanical specimens are stored in fluid preservation by immersing in liquid in a labeled bottle.
Liquid Preservatives
FAA1 (formalin aceto-alcohol): 50 parts 95% ethyl alcohol + 40 parts distilled water + 5 parts glacial acetic acid + 5 parts 38% formalin
FAA2: 90 parts 70% ethyl alcohol + 5 parts 38% formalin + 5 parts glacial acetic acid
What is a Plant?
Organisms that perform photosynthesis, possess cell walls, spores, and sedentary behavior
Land plants include trees, herbs, bushes, grasses, vines, ferns, and mosses.
The Evolution of Life
Archaea and Bacteria: small, mostly unicellular organisms with circular DNA, replicate by fission, and lack membrane-bound organelles.
Eukaryotes: unicellular or multicellular organisms with linear DNA, replicate by mitotic and meiotic division, and possess membrane-bound organelles.
Land Plants Green Plants (Chlorobionta)
Characterized by unique chloroplasts with specific pigments, thylakoid structures, and storage compounds.
Comprise aquatic green algae and land-dwelling embryophytes (Embryophyta).
Land Plants (Embryophyta):
Protective outer cuticle against desiccation
Specialized reproductive organs (gametangia) with sterile cell layers
A life cycle with an intercalated diploid phase and an embryo stage.
Systematics
Science including traditional taxonomy (description, identification, nomenclature, and classification of organisms), reconstructing phylogeny or evolutionary history
Founded on evolution, major premise being that there is one phylogeny of life.
Evolution
Change since the origin of the universe (15 billion years ago).
Biological evolution: descent with modification (Charles Darwin).
Mechanism of Evolution
Genetic Drift: Frequency of traits changes in population due to chance events (random change).
Natural Selection: Traits that improve survival or reproduction accumulate in the population (adaptive change).
Taxonomy
Taxa (taxon) – group of organisms
Description
Identification
Nomenclature
Classification
Description
Assign features to a taxon
Features are called characters
Two or more forms of a character are character states
Example:
CHARACTER: PETAL COLOR
CHARACTER STATE: Yellow and Blue
Plant Identification
Basic process of classification
Direct comparison of an unknown specimen with the already classified, circumscribed and named taxa
Determination of similarities or differences:
Direct comparison of the features of a specimen in hand with those in keys in order to arrive at a name
Assignment of an unidentified taxon to the correct class in an established system of classification
Plant Identification Includes the use of keys
computer-based methods
polyclave-type devices
Characters Considered Before Plant Identification:
Herbaceous or woody, and annual or perennial nature.
Leaf type, phyllotaxy and venation
Stipule presence/absence and type.
Milky or colored sap presence.
Distribution and kinds of surface coverings (hairs, trichomes, spines).
Observe the flowers and name its parts
Count the number of sepals and petals and also their arrangement
Note whether perianth is present in one series, more series or absent
Note whether the sepals and petals are separate or fused
Note whether pappus (Asteraceae) or epicalyx (Malvaceae) are structures are present
Note whether a nectar-secreting disc is present in the flowers (Rutaceae)
Determine whether the flowers are actinomorphic or zygomorphic
Number and attachment of stamens; any fusion of anthers or filaments
Stamens –antipetalous, alternipetalous, or obdiplostemonous
Count the number of pistils, styles and stigmas of the gynoecium
Cut the transverse section of the ovary, count the number of locules, number of ovules per locule and also observe the placentation
Longitudinal section of flower – position of the ovary and fusion of the perianth
Determine the number of carpels
After carefully examining all the above characters, the next step in identification is KEYING
Identification with Keys
KEY: artificial arrangement providing a choice between contradictory statements.
COUPLET: a single pair of contradictory statements in a key.
LEAD: each statement of a couplet.
Leads are usually the best contrasting characters
Characters following the lead are called secondary key characters
Keys: Punched cards keys and Dichotomous keys
Punched Cards Keys
Cards with names of all taxa (species, genera, or families).
One corner of each card has a character and number.
Taxa showing character are perforated in front of their names.
Taxa lacking character are without perforation.
Number of cards and characters are the same
For identifying a plant, select cards showing the plant's characters.
Combination of characters will allow only one perforation.
Plant is referred to the family on that card.
Dichotomous Keys
Pairs of contrasting characters or couplets, each statement of which is a lead
Both leads are numbered, and begin with the same word as much as possible.
Types of Dichotomous Keys:
INDENTED OR YOKED KEY: each couplet is indented a fixed distance from the left margin.
BRACKET OR PARALLEL KEY: couplets are next to each other in consecutive lines.
Some Unconventional Identification Methods
POLYCLAVE IDENTIFICATION: choice of several characteristics; multi-entry, order-free key in several formats.
User chooses any character, in any order or sequence.
Diagnostic key—cards are utilized.
Computer-stored multientry key.
Printed table—gives status of different taxa and characters.
Computerized Identification
Machines planned only for the evaluation of a computer programme or algorithm
An algorithm is a series of logical steps or instructions by which an identification can be made
Four Major Approaches:
Computer-constructed keys
Computer-stored dichotomous keys
Automated pattern-recognition systems
Simultaneous character-set method
Other Identification Methods:
Chromatography
Spectroscopy
Molecular studies
CHROMATOGRAPHY: used to monitor reaction progress and identify compounds.
SPECTROMETRY: emerging method for characterizing proteins.
MOLECULAR SYSTEMATICS: identifying genes (DNA).
Plant Nomenclature
Assignment of definite names to plants
Involves the principles governed by rules formulated and adopted by International Botanical Congresses
Listed formally in a code – International Code of Botanical Nomenclature (ICBN)
ICBN
Major goal: provide one correct name for each taxon.
Taxa: taxonomic groups of any rank.
Nomenclaturist
Taxonomist assigning names to new taxa and validates the right names for old taxa per classifications
Scientific Names
Universal and recognized throughout the world
Provide information regarding generic and family relationships
A well-known plant has only one scientific name
Two or more plants always have different scientific names
All known plants have a scientific name
Binomial System of Nomenclature
Carolus Linnaeus (1753)
Two names: genus & species names
Latinized words
Saccharum officinarum
Genus Name
Always a noun
Written with a capitalized initial letter and the remainder small
Types of origin:
a. Names of well-known persons (Candollea – in honor of A.P. de Candolle)
b. Descriptive with some common characteristics of the included species (Cercocarpus – coiled fruit)
c. Poetic or mythological origin (Theobroma – God’s food)
d. Aboriginal name of the plant (Betula and Quercus – old Greek names for Birch and Oak)
Specific Epithet
Written with a small initial letter
Derived from person’s name – the initial letter of the epithet may be a capital letter
Often an adjective
May be a name in honor of a person
May be derived from a geographical location
May originate from an old common name
May be derived from some characteristics of the plant
May also be named arbitrarily
The authority, written after the specific epithet, is never underlined
Typed or handwritten – genus and species names should be underlined (separate lines)
Should be printed in italics or boldface (genus and species)
Incomplete if not followed by full or abbreviation name(s) of the author (s)
Pyrus malus L.
To verify the date or time of the first valid publication of the name of a particular taxon
ICBN
Linnaeus – proposed the elementary rules of naming plants in his Philosophia Botanica
A.P. De Candolle – detailed set of rules regarding plant nomenclature in his Theorie elementaire
Alphonse de Candolle – convened the First International Botanical Congress in Paris (1867)
Set of rules of plant nomenclature – most of which were proposed by A. De Candolle
De Candolle Rules or Paris Code of 1867
1930 – Code of Nomenclature – International Code of Botanical Nomenclature
This edition of the Code embodies the decisions of the Nomenclature Section of the XIX International Botanical Congress (IBC), which took place in Shenzhen, China in July, 2017.
This Shenzhen Code supersedes the Melbourne Code (McNeill & al. in Regnum Veg. 154. 2012), published after the XVIII IBC in Melbourne, Australia in 2011.
ICBN/ICN
Divided into three parts:
Principles
Rules
Recommendations
Philosophical Basis of ICBN
Six Principles:
Botanical nomenclature is independent of zoological nomenclature
The application of names of taxonomic groups is determined by means of nomenclatural types
The nomenclature of a taxonomic group is based upon priority of publication
Each taxonomic group with a particular circumscription, position, and rank can bear only one correct name, the earliest that is in accordance with the rules, except in specific cases
Scientific names of taxonomic groups are treated as Latin regardless of their derivation
The rules of nomenclature are retroactive unless expressly limited
Rules and Recommendations of ICBN
Object of the Rules: Ordering nomenclature and prevent names contradicting rules
Recommendations: Policies to produce uniform clarity, especially in nomenclature and future nomenclature
Rules and Recommendations of ICBN: all organisms treated as plants (including fungi, excluding bacteria)
Recent Code edition: supersedes all previous editions
Some Important Rules of Nomenclature
RANKS AND ENDINGS OF TAXA
Species: basic classification unit
Acceptance of grammatical endings/suffixes is provided by ICBN (1983)
Endings Examples:
Division: -phyta, Magnoliophyta
Subdivision: -phytina, Pterophytina
Class: -opsida, Pteropsida
Subclass: -opsidae, Pteropsidae
Order: -ales, Rosales, Asterales
Suborder: -ineae, Rosineae
Family: -aceae, Rosaceae
Subfamily: -oideae, Rosoideae
Tribe: -eae, Roseae
Subtribe: -inae, Rosinae
Genus: -us,a,um, -on, es, Rosa, Aster, etc
Principle of Priority
Each taxon is known by its earliest name
Cleome gynandra Linn (1753)
Cleome pentaphylla Linn - Linnaeus changed its name
1824 – De Candolle recognized 3 separate genera (Cleome, Polanisia, and Gynandropsis)
1960 – Iltis merged Gynandropsis and Cleome into one genus
Citation of Author
ORIGINAL AUTHOR: Complete and accurate name of author publishing the name
Liliaceae Adans ; Lilium superbum Linn.
JOINT AUTHOR: Names of both authors should be cited (et or & )
Illicium griffithi Hook & Thorns ; Illicium griffithi Hook et Thorns
RANK ALTERATION: Name of first author cited in parentheses, the alterer second.
Allioni raised the rank of variety Medico polymorpha var. orbicularis L. to the species rank
Medico orbicularis (L.) All.
NAME PROPOSAL: Word ex between the former author and subsequent author
Gossypium tomentosum Nutt ex Seem.
Type Method
Legal device to provide correct name for a taxon
Type specimen – a herbarium sheet used by the author to provide its authentic description
HOLOTYPE: specimen used by the author in the original publication
ISOTYPE: duplicate of the holotype
LECTOTYPE: Selected by a competent worker when no holotype or destroyed holotype
NEOTYPE: serves as substitute for the holotype when all name-associated material is missing
NOMENCLATURAL TYPE:
Element with which the name of a taxon is permanently associated
Synonyms and Related Definitions
SYNONYM: Name rejected for reason in taxonomic judgement
BASIONYM: Specific or intraspecific with priority when transferred.
HOMONYM: Identical names based on different types of which only one can be a legitimate name.
TAUTONYM: An illegitimate binomial in which the genus/species name is the same
Armoracia armoracia (L.) Britton
AUTONYM: Automatically created legitimate tautonym for infraspecific or infrageneric taxa
Hypericum subgenus Hypericum section Hypericum
Latin Diagnosis
The diagnosis, details and description of new taxa published before 1st January 1955 were accepted by ICBN as valid, irrespective of the language.
After this date, the description of any newTaxa would be considered valid only if accompanied by Latin diagnosis
Effective and Valid Publication
Effective & considered valid when distributed (printed) to general pubic that reaches established institutions.
Choice of Names When The Taxon Rank is Changed
When a taxon rank is changed, the legitimate name in its new rank is its correct name
Choice of Names When Sub-Rank Taxa are United
When ranked and united: Oldest Taxa name = united Taxa name
Retention of Names of Divided Taxa
When a genus/species is divided, the original name: genus/species
Retention of Names of Taxa on Transference
Transferred subdivision of genus (w/o changing rank), original legitimate name of taxa must be retained
Rejection of Names
Illegitimate (tautonym or homonym).
Permanent source of confusion.
Derived from 2+ elements.
Coincide with morphological term, are unitary designations to species, or words not intended as names.
Names of Different Taxa
ICBN (1983)
Genera/Higher ranks: monomials.
Aesculus L., Rosaceae Juss.
Species: binomials.
Gossypium tomentosum Nutt.
Subspecies: trinomials.
Hibiscus moscheutos ssp. palustris (L.) Clausen.
Varieties should be quadrinomials
Lilium catesbaei Walter ssp. catesbaei var. longii Fernald
Botanical Names
Latin (or latinized words)
Common prefixes
Numbers: uni-, bi-, tri-, quadri- etc.
Greek numbers: mon-, di-, tri-, tetra- etc.
Common Suffixes Used in Specific Epithets
-aceous, -alis, -aris, -arium, -aticus, -estris, -eus, -ilis, -osus
Plant Parts Used as Epithets
receptaculum, sepalum, ovarium, carpellum, pedicellus, loculus, pistillum, stylus, fructus, petalum, folium/phyllon, discus, rhiza, caulos
Specific Epithets Linked with Color
albicans, albus, atrovirens, aureus,
calcareus, candidus, croceus, flavidus,
flavus, fulvus, glaucus, niger,
purpureus, violaceous, viridis
Specific Epithets Linked with Geography
africanus, americanus, arabicus, argentinus,
asiaticus, australiensis, brasiliensis, canadensis,
chinensis, cubensis, germanicus, indicus,
sinensis, philippinensis, zeylanicus, mexicanus
Specific Epithets Linked with Size
altus, exaltatus, giganteus, grandis, humilis, major, minor, minutus, procerus, pumilus, robustus
Specific Epithets Linked with Habit
arborescens, dichotomus, erectus, furcatus, prostratus, ramosus, repens, stoloniferus
Specific Epithets Linked with Habitats
amphibius, aquaticus, arvensis, campestris, hypogeus, lacustris, littoralis, maritimus,
montanus, palustris, rupestris, sativus, sylvaticus, sylvestris, terrestris
Plant Classification
Placement of plants into groups or categories for a clear understanding, proper study and effective organization
Ranks of Plant Classification
Species
Genus
Family
Order
Class
Division/Phylum
Ranks of Plant Classification Subcategories
Species : Subform, form, subvarieties, varieties, subspecies
Genus: Subsection, section, subgenus
Family: Subtribe, tribe, subfamily
Order: Suborder
Class: Subclass
Division: Subdivision
Types of Systems of Classification
Artificial classification Habit and importance
Theoprastus, Secundus, Dioscorides, Magnus, Brunfels, Bock and Fuchs
Mechanical classification Few selected taxonomic characters
Caesalpino, Bauhin, Tournefort, Linnaeus
Natural classification Used as many taxonomic characters as possible
Adanson, de Jussieu, de Candolle & son Alphonse, Bentham & Hooker
Phylogenetic classification Used as many taxonomic characters in addition to the phylogenetic (evolutionary) interpretations
Eichler, Engler, Prantl, Bessey, Takhtajan, Wettstein, Hallier, Hutchinson, Cronquist, Dahlgren and Thorne
Some Important Systems of Classifications John Ray – Methods Plantarum (1703)
Form of relationships
Grouping together plants that resembled one another
He was the first one to divide herbs, shrubs and tress into dicotyledons and monocotyledons based on the presence of one or two cotyledons
Some Important Systems of Classifications
Flowering Non flowering
Monocotyledons: DICOTYLEDONES MONOCOTYLEDONES
Monocotyledons: MONOCOTYLEDONES DICOTYLEDONES
Most prodigious systematist of all times
Born on May 23, 1707 in Sweden
1737 – finished medicine from the University of Harderwijk of Netherlands
He became the personal physician of a wealthy banker George Clifford
Some Important Systems of Classifications Clifford was interested in plants
Linnaeus remained in Netherlands for three years
He travelled in Europe and collected plants
1741- he returned to Sweden and became the professor of Botany and Medicine at the University of Uppasala
WORKS:
SYSTEMA NATURAE – contains the outline of his system of classification
GENERA PLANTARUM – contains the description of several genera
SPECIES PLANTARUM – two volumes; contains his detailed views on plant identification and description of species
Plant collections of Linnaeus was sold by his wife to J.E. Smith (British botanist)
J.E. Smith – one of the founders of Linnean Society of London
Entire plant collection – Office of the Linnean Society in Burlington House, Piccadilly, London
Linnaeus’s system of classification – artificial sexual system
Radford (1986) – described it as mechanical system of classification
Linnaeus divided plants into 24 classes mainly on the bases of number, union and length of stamens
Dominant for seven decades
Linnaeus divided the classes into orders
Algae, fungi, mosses, and ferns are under one class Cryptogamia (based on the number of styles)
Linnaeus classification lasted seven decades
Replaced by natural classification systems of A.L. de Jussieu and A.P. de Candolle on form relationships
Some Important Systems of Classifications Antoine-Laurent de Jussieu
Natural system of classification (Genera Plantarum Secundus Ordines Naturales Disposita)
Recognized 100 orders of the plants which are now called families
Divided the plants into three main groups: Acotyledones, Monocotyledones and Dicotyledones
Used number of cotyledons, presence or absence of cotyledons, number of petals, presence or absence of petals and position of stamens
Agustin Pyramus de Candolle Natural system of classification (Theorie Elementaire de la Botanique)
Divided plants into two major groups: Cellulares (non-vascular plants) and Vasculares (vascular plants)
Prodromus – described all the species of the vascular plants
Large genera are divided into section and subsections
Some Important Systems of Classifications George Bentham & Joseph Dalton Hooker Most accepted natural system of classification (Genera Plantarum – contains description of all known genera of seed plants published in Latin)Divided all Phanerogams or seed plants into Gymnosperms, Dicotyledons and Monocotyledons
August Wilhelm Eichler Transitional phylogenetic system of classification
Non-seed and seed plants
Gymnosperms and Angiosperms
Adolf Engler and Karl Prantl Transitional phylogenetic system Radford (1986) – phylogenetic system Provided keys and descriptions of all the plant families known to them at that time Classified all the plants from algae to angiosperms
Engler and Prantl: Placed monocots before dicots; considered orchids more evolved than grasses; divided plant kingdom into 14 divisions
Charles Edwin Bessey Pure phylogenetic system of plant classification classifications
Ranalian concept of evolution
* Ranales were the primitive angiospermsOne branch of Ranales developed into monocots and the other into dicots
John Hutchinson Phylogenetic system
Genera of Flowering Plants and Families of Flowering Plants
Shrubs & tress are primitive than herbs in any one genus or family *Perennial are older than annuals, biennials
Aquatic flowering plants derived from terrestrial ancestors
* Dicots are primitive compared to monocots
* Simple leaves are primitive than compound leaves
* Dioecious plants are more recent than monoecious
* Inflorescence is more advanced than the solitary flowers
* Apetalous flowers are derived from petals flowers
* Aggregate fruits are more evolved than single fruits
* Polypetaly is more primitive than gamopetaly
* Actinomorphy is more primitive than zygomorphy
*Apetalous flowers are derived from flowers with petals
**Current systems of classifiers, Armen Takhtajan proposed a new classification with Anatomy, Embryology and chemistryRobert Thorne. Ultastructure embryology morphology seed and pollen Morphology host. Monophyletic origin of angiospers
Eduardo Quisumbing Phd. Was plant taxonomy Systematic and morphology Saccolabium quisumbingii has been named in his honorLeonardo Co Filipino. discovered eight new plant of species Philipphine Native Plants Conservation. Mycaranthes leonardi, Rafflesia leonardi Orchid. 3rd Largest flower in world
Edwino Fernsndo. revision of GENus nenga In southeAst and palms of the region and contributed to our knowledge. Knowledge of plants survery discovery
There is Cytotaxonomy Chemotaxonomy Molecular classification
External morphology relations to the taxonomy.Easily used and observable most and frequently used and applied Morphology vegetative character growth patterns. Floral character Floral structure. Inflorescence.
Vegetatice
EPIDERMIS Shape Wall Thickness Inculsion Occlusion Distrinution. Trichomes Size Function Relation with all structures
Motor cell grass. specialised cells. Sclereids rarely contain except families that contain
Size function cell wall patterns Pitting of of Vessel element and wall
Relation to wood. Different charactersMicrospore Megaspore gametophuyte development. Anther type the formation of endosperms embryos, seed coats
Chemotaxanomy application of chemistry with test
Ecology relation. the distribution adaption. Below the genes that areMicrofossils Macro Evolution of fossils.
Understanding of relationsPollu
Ecology. Biology to understand and develop and evolve Genetic
A new type of relation reproduction
Flowering the of traits and adaptationbreeding and Timing. the over all time frame semiparous and iteroparous.
Molecular sequence
Numerical value
*Chemotaxonoy
On basis of protein Serotaxanory for identification
Evolution origin of the life. Modifications of genes with transfer and of species
Synapomorphies and autapomorphic relation and