PCR LAB

  1. Cheek Cell Collection - The process involves rinsing with saline and centrifuging to form a cell pellet.

  2. DNA Release Method - Heat and Chelex are used to lyse cells, releasing the DNA.

  3. PCR Purpose - PCR amplifies the TAS2R38 gene to increase the quantity of specific DNA regions.

  4. Restriction Enzymes Function - They cut amplified TAS2R38 DNA based on specific SNPs in the nucleotide composition.

  5. Gel Electrophoresis - Used for visualizing DNA segments post-restriction enzyme treatment.

  6. Homozygous Non-Taster - Results in one large DNA cluster.

  7. Homozygous Taster - Results in two distinct DNA clusters.

  8. Heterozygous Taster - Results in three separate DNA clusters.