Energy Systems Overview
Mitochondria
- Powerhouse of the cell.
- Generates ATP through aerobic/cellular respiration.
- Self-replicates during high demand.
- Important in apoptosis.
- Cristae increase surface area for ATP production.
ATP
- Energy currency.
- Links energy-yielding and requiring functions.
- Carbs + Fats + Proteins \rightarrow ATP \rightarrow Physiological functions + Heat
- ATP \rightarrow ADP + Phosphate
Metabolism
- Energy transformation in the body.
- Energy cannot be created or destroyed, only transformed.
Catabolism
- Energy releasing.
- ATP \rightarrow ADP + Phosphate + Energy
Anabolism
- Energy requiring.
- ADP + Phosphate + Energy \rightarrow ATP
Macronutrients
- Carbohydrates (CHO): Rapid energy source (glucose, muscle glycogen).
- Fats (CHO): Prolonged exercise energy (triglycerides in adipose tissue).
- Proteins: Limited energy use, not efficient.
Cellular Respiration
- Metabolic pathway using glucose to produce ATP in mitochondria.
- Three steps:
- Glycolysis.
- Citric acid cycle (Krebs cycle).
- Electron transport chain.
- Net ATP gain: 30-32 per glucose molecule.
Glycolysis
- Occurs outside the mitochondria (in the cytosol).
- Anaerobic.
- Net gain: 2 ATP + 2 NADH + H + 2 pyruvate.
- Rate-limiting enzyme: Phosphofructokinase.
Krebs Cycle (Citric Acid Cycle)
- Aerobic (requires oxygen).
- Occurs in the mitochondria.
- Net gain: 2 ATP + NADH + FADH + 4 CO2.
- Rate-limiting enzyme: Isocitrate dehydrogenase.
Electron Transport Chain
- Aerobic.
- Occurs in the mitochondria.
- Net gain: 26-28 ATP.
- Rate-limiting enzyme: Cytochrome oxidase.
Intracellular Regulators
- Rate-limiting enzymes: Phosphofructokinase, isocitrate dehydrogenase, cytochrome oxidase.
- Stimulators: Increase ATP production (e.g., AMP, ADP, Phosphate, higher pH).
- Inhibitors: Decrease ATP production (e.g., ATP, Creatine Phosphate, Citrate, lower pH).
- Phosphorylase breaks down glycogen into glucose.
Extracellular Regulators
- Hypothalamus stimulates the sympathetic nervous system.
- The pancreas decreases insulin and increases glucagon.
- Increased epinephrine and norepinephrine to stimulate ATP production.
Hormonal Regulators
- Key hormones: Glucagon, epinephrine, norepinephrine, growth hormone, cortisol.
- Secondary hormones: Growth hormone-releasing hormone, thyroid-releasing hormone, thyroid-stimulating hormone, corticotropin-releasing hormone, adrenocorticotrophic hormone.
Gluconeogenesis
- Creation of new glucose from non-carb sources (occurs in the liver).
- Triglycerides to glycerol to glucose.
- Lactic acid/pyruvate to glucose.
- Alanine to pyruvate to glucose (produces ammonia).
Cyanide and Cellular Respiration
- Cyanide poisons the electron transport chain.
- Terminates the ability to generate ATP aerobically.
- Elevated lactic acid levels postmortem.
- Venous oxygen saturation is greater than 90%.
Exercise Metabolism Goals
- Mobilize free fatty acids.
- Decrease glucose uptake in non-working muscles.
- Provide glucose for the nervous system.
- Increase breakdown of liver and muscle glycogen.
Energy Continuum
- ATP PC system: Predominates early (activities < 10 seconds).
- Lactic anaerobic system: Predominates for activities around 2 minutes.
- Aerobic system: Predominates for activities > 2 minutes.
Fuel Utilization
- Fats: Dense energy source for prolonged periods.
- Carbs: Readily available, but don't last as long.
ATP PC System
- Immediate energy source.
- Uses stored phosphocreatine.
- PC stores supply about 10 seconds of all-out activity.
- Replenishment: 50% in 30 seconds, complete in 3-5 minutes (passive recovery).
- Trained individuals replenish PC stores faster than untrained.
Lactic Anaerobic System
- Uses stored glycogen.
- Fate of pyruvate dictates anaerobic glycolysis.
- Low oxygen leads to lactic acid production.
- Lasts up to roughly 2 minutes.
- Muscle contraction and nervous system stimulates are triggers.
Mechanisms of Lactic Acid Production
- Muscle contraction leads to glycogenolysis which is processed via anaerobic glycolysis.
- Sympathetic nervous system stimulates epinephrine and glucagon release.
- Enzymes- Lactate dehydrogenase (LDH) catalyst pyruvate conversion in lactic acid
- Type ll muscle fibers stimulate an increased LDH activity, an increase in lactic acid production.
Lactic Acid Clearance
- Oxidation (use it again).
- Gluconeogenesis (non-carb sources).
- Transamination (breakdown of amino acids).
- Intracellular (within cell) and extracellular (between cell) mechanisms.
Intracellular Lactate Shuttle
- MCT1 transports lactate from cytoplasm into mitochondria.
- Lactate converts to pyruvate, enter glycolysis.
Extracellular Lactate Shuttle
- MCT4 clears lactate from fast glycolytic fibers.
- Transport into live for gluconeogenesis
- Lactate is preferred fuel for cardiac muscle during exercise.
Fuel from Glucose
- The primary and most easily utilized source og ATP production.
Glycogen
- comes from either liver or muscle, gets broken down into glucose, phosphoglyceraldehyde, pyruvic acid, and acetyl CoA,
Proteins
- Amino acids get broken down by oxidative deamination or transamination, main linker.
Triglycerides
- Broken down into glycerol and our fatty acids, leading them to acetyl coenzyme A.
Lipolysis
- Breakdown of stored fat.
- Mediated by hormone-sensitive lipase (HSL).
- HSL release triggered by increased glucagon, mediated by epinephrine, triggered by sympathetic nervous system.
Beta Oxidation
- Breakdown of free fatty acids into acetyl coenzyme A.
- Occurs in mitochondrial matrix with the presence of O2.
- Five step five step process with 5 mechanisms.
- Cycle through carbon bond which is broken
Palmitate Example (16-Carbon Fatty Acid)
- Cycles through beta oxidation seven times.
- Each cycle produces 1 FADH and 1 NADH+ (4 ATP yield).
- 8 Acetyl CoA enzymes are yielded in this breakdown.
- =106 net ATP from 16 C units.
Crossover Concept
- At rest/lower intensity: Fat predominates.
- Higher intensity: Carb contribution increases, decreased fat.
Protein Metabolism
- Transamination: Transfer of amino group from amino acid to keto acid.
- Oxidative Deamination: Amino group removed, becomes ammonia (toxic).
- Gluconeogenesis: Alanine converted to glucose in liver.
- Amino acid derivatives utilized as pyruvate or acetyl CoA.
- Maintains blood glucose during prolonged aerobic exercise.
- Trained individuals utilize ketones as fuel better.
Oxygen Deficit
- At the onset of exercise, energy demand increases faster than oxygen supply.
- Mechanisms:
- Inertia of metabolism.
- Inadequate initial oxygen supply.
- Short-term light to moderate submaximal aeorbic exercise show that our aeorbic system predominates
- Short-term high-intensity anaeribic exercise show that 02 is larget O2 Defciit.
EPOC (Excess Post-Exercise Oxygen Consumption)
- Transition from exercise to rest.
- Causes: ATP PC restoration, replenished O2 stores, elevated cardio-respiratroy function, hormones/body temp, lactate clear, substrate. shift to carbs/fat.
* body temperature 60-70% oof contribition towards EPOC
# Magnitude of increase factor intesnity over duration.
Lactate
*Baseline of 0.6-1.0
- level is intensity over duration
- Steady State, tells us that rate is being meet
- High Lactates indicate that demand is not being met
Lactate Threshold
*Point on the Linear Cumlinar continumm
*Two Sharp rises on Lactate accumulation, also called Threshold 1,2
Lactate Threshold 1, (LT1)
*First noticeable rate, but effetivelly effectivel clear and retain areobic metabolism
Lactate Threshold 2, (LT2)
- Second noticeable spike and greaty production than clears, anerobic production dominant
Oxygen Consumption (Aerobic Response)
- Oxygen consumption plateau is when demand = supply
Oxygen Drift
- Increase in use, even what the amount stays the same
- workload is 0-70% VO2 Max capacity.
- #5 mechanisms, high EP/NOREP increase in lactate accumulation in substrate utlitlizaation increased of ventilation, body tempt,
VO2
*Definied by maximum oxygen indiduals consume, transport and to prodcue ATP, Aerobicaly
Respiratroy Exchange Ratio (REE)
Def: Carbon Dioxide/ VO2 relationship.
*Lower Numbers show utlizesation of fats, hgiher numbers, utilization of carbon, 10-% fat with AER = 0.7.
Metabolic Adapations
- Sustrate fuel supply
- Enxyme Activity
3 O2 ultiZATION
4 Lactated Accumulation - ATP prod, storage and turnover
Metabolic Adaapatations- Fuel and Enzyme Supply
With any sort Prolong adaeorpic training, any cardiovasular exercise/cardisivasula period of time witnes
1.Decrease Use of fuel.
2 Improve store glycogen. reserve to use. More effectivley to get to Beta 02 or get new glucose source
Enxzyme= improve acvtivity larger and and number activity greater cycilc through. Increase acticcy shuttle for efffectively
Metabilac adaaptions 02 Ultilatizatiion
- impove VO2 MAx with lower deficit and oxygen and EPO
*Decrease lactate due to enzyme capacity
Metabllic ADAPTATIONS- ATP
ATP is not better. In the same substrate increase size and number and capacity store larger size in much better turn over, can refill quickler
Detraining
*Factore that improve, decline. When stop Metabilocal decrease , if the not the same thing, the metabolica falter
- Some reading to prompt and queriing further learning.