bio wai

Chapter 1: Cell Structure and Organization

The Cell Theory

  • Basic Unit of Life:

    • The cell is recognized as the basic unit of structure and organization in living organisms.

    • All organisms consist of one or more cells.

    • Cells arise from pre-existing cells.

What Are Cells?

  • Cells are the building blocks of life and possess all characteristics of life.

Types of Cells:

1. Animal Cell

  • Organelles include:

    • Endoplasmic Reticulum

    • Cytoplasm

    • Mitochondrion (plural: mitochondria)

    • Nucleus

    • Golgi Body

2. Plant Cell

  • Organelles include:

    • Cell Wall

    • Large Central Vacuole

    • Chloroplast

    • Cell Surface Membrane

Key Functions of Cells

  • Red Blood Cells (RBC):

    • Perform respiration to release energy.

    • Structure aids binding and transport of oxygen (no nucleus, biconcave shape).

  • Root Hair Cell:

    • Increase surface area for absorption of water and minerals.

Cell Function and Structure Relation

  • Differentiation:

    • Allows cells to specialize and adapt for specific functions (e.g., muscle cells vs. nerve cells).

Characteristics of Protoplasm

  • Living material in cells consists of three parts:

    • Nucleus

    • Cytoplasm

    • Cell Surface Membrane

Cell Surface Membrane

  • Also known as the plasma membrane.

  • Partially Permeable:

    • Controls the exchange of substances in and out of the cell.

Nucleus

  • Contains chromatin (DNA structure) which carries hereditary information.

  • Surrounded by a nuclear envelope and contains nucleoli (sites of ribosome production).

Endoplasmic Reticulum (ER)

  • Rough ER: Contains ribosomes for protein synthesis.

  • Smooth ER: Synthesizes lipids and detoxifies certain chemicals.

Golgi Body

  • Chemically modifies and packages substances for secretion.

Proteins

  • Function in energy sources, synthesis of antibodies and hormones, and enzymes (e.g., amylase).

Carbohydrates

  • Organic molecules primarily composed of carbon, hydrogen, and oxygen.

    • Classified into:

      • Monosaccharides: Simple sugars (e.g., glucose).

      • Disaccharides: Composed of two monosaccharides.

      • Polysaccharides: Long chains (e.g., glycogen, starch).

Food and Nutrition

  • Nutrients provide energy for vital activities and maintain health.

Malnutrition

  • Causes include imbalances or deficiencies in the diet.

    • Marasmus: Severe nutrient deficiency.

    • Kwashiorkor: Severe protein deficiency.

Page 2: Food Tests and Nutrient Identification

Test for Reducing Sugars

Reagent: Benedict's Solution

  1. Add 20 cm³ of liquid food sample to a test tube.

  2. Add 2.0 cm³ of Benedict's solution and shake.

  3. Boil in a water bath for 2 minutes.

Observations & Results:

  • Blue solution: Reducing sugar absent.

  • Green to brick red precipitate: Indicates amounts of reducing sugars.

Test for Fats

Reagent: Ethanol

  1. Crush solid food sample and mix with ethanol.

  2. Shake and decant.

  3. Add water.

Observations:

  • Clear mixture: Fat absent.

  • Cloudy white emulsion: Fat present.

Test for Proteins

Reagents: Biuret Solution

  1. Add 2.0 cm³ of liquid food sample to a test tube.

  2. Add Biuret solution drop by drop and shake.

Observations & Results:

  • Blue solution: Protein absent.

  • Violet solution: Protein present.

Functions of Proteins

  • Source of energy, aid in synthesis of protoplasm, antibodies, hormones, and enzymes.

Starch Test

Reagent: Iodine Solution

  1. Prepare crushed sample and add iodine.

Observations:

  • Yellow-brown: Starch absent.

  • Blue-black: Starch present.

Nutritional Sources of Fats and Proteins

  • Animal Sources of Proteins: Dairy products, meat, eggs.

  • Plant Sources of Proteins: Soybeans, nuts.

  • Fats Classification:

    • Saturated: Found in animal fat, raises cholesterol levels.

    • Unsaturated: Found in plants (e.g., olive oil).

Structure of Proteins

  • Made from long chains (polymers) of amino acids, formed by peptide bonds.

  • Can denature when exposed to heat or acidic conditions.