1.05 - Introduction to viruses Dr Lauren McNeill

Page 1: Introduction

  • Dr. Lauren McNeill is a Lecturer in Biosciences and Deputy Director of Admissions at Lancaster University.

  • Overview of the lecture on viruses.

Page 2: Aims of the Lecture

  • Review important infectious pathogens and sources of infections.

  • Define and understand viruses as unique microorganisms.

  • Discuss classification methods for viruses.

  • Explore the diseases caused by viruses.

  • Discuss treatment options for viral infections.

  • Consider future implications of viral diseases.

Page 3: Quick Recap

  • Summary of points covered regarding viruses.

Page 4: Sources of Infection

  • Endogenous Sources: Infections from the host's normal flora.

  • Exogenous Sources: Infections transmitted from an external source.

Page 5: Important Infectious Pathogens

  • Categories of infectious pathogens include:

    • Bacteria

    • Viruses

    • Fungi

    • Parasites

Page 6: Focus on Viruses

  • Specific focus on viruses within the medical curriculum at Lancaster University Medical School.

Page 7: Commonality of Individuals

  • Notable historical figures: Mary Berry, Franklin D. Roosevelt, Jack Nicklaus, and others.

Page 8: Polio Overview

  • Polio was eliminated in most countries since effective vaccines were developed in the 1950s.

Page 9: Details on Polio

  • Polio is caused by the Picornaviridae RNA virus:

    • Transmitted via faecal-oral route.

    • Primarily affects children under 5 years.

    • 1 in 200 infections can lead to irreversible paralysis.

    • WHO aims for polio eradication by 2026.

Page 10: Historical Figures

  • Commonality of notable figures: Elizabeth I, Abraham Lincoln, Joseph Stalin, etc.

Page 11: Smallpox Eradication

  • Smallpox was eradicated, with the last wild case in 1977; remaining strains kept in labs.

Page 12: Links Between Figures

  • Questioning what links the discussed individuals.

Page 13: Rabies Overview

  • Classical rabies virus, carried by warm-blooded animals, results in fatal encephalomyelitis once symptoms appear.

  • Rhabdovirus infection via infected animal bites.

Page 14: Pandemic Impact

  • Inquiry into death tolls surpassing those of World War I.

Page 15: Spanish Flu Pandemic

  • Spanish Flu (1918-1919) infected approximately 1/3 of the global population, causing more deaths than WW1.

Page 16: Child Mortality Statistics

  • Investigation on what caused more child deaths than road accidents in 2019.

Page 17: Measles Outbreak in Samoa

  • 2019 outbreak: 6000 cases and 83 deaths due to declined MMR vaccine uptake.

  • Vaccination programme reinstated, bringing the outbreak under control.

Page 18: Measles Overview

  • Measles caused by Morbillivirus:

    • Transmitted via the airborne route.

    • Highest mortality among children under 2 years.

    • Natural infection leads to lifelong immunity.

Page 19: Annual Case Statistics

  • Influenza has around 1 billion cases annually.

Page 20: Seasonal Influenza Statistics

  • 3-5 million cases of severe illness; 290,000 to 650,000 respiratory deaths annually.

  • Disproportionate death rates in young children in developing countries.

Page 21: Importance of Viruses

  • Viruses play a significant role in global health issues.

Page 22: Structure of Viruses

  • Introduction to basic virus structure and components.

Page 23: Basics of Virus Structure

  • A virus particle, or virion, usually has a viral genome in a protein capsid.

  • Viral genomics are crucial for infection and classification processes.

Page 24: Virus Structure Details

  • Viruses are metabolically inert and lack cellular structures.

  • Differentiation between enveloped and non-enveloped viruses.

Page 25: Important Notes on Viruses

  • Key points:

    • Viral genomes only function post-infection.

    • Viruses exploit the host's protein synthesis pathways.

Page 26: Virus Classification

  • Viruses classified by several factors:

    • Type of nucleic acid in the genome.

    • Size, structure, symmetry of the virion.

    • Replication methods and nucleic acid strands.

Page 27: Classification Specifics

  • Key elements for viral classification:

    • Type of nucleic acid (DNA or RNA).

    • Virion size/structure and replication mode.

Page 28: Viral Genomes Types

  • DNA Viruses: Typically double-stranded (except Parvovirus).

  • RNA Viruses: Generally single-stranded, with exceptions (e.g., Reovirus).

Page 29: Baltimore Classification of Viruses

  • Seven groups based on nucleic acid types and other characteristics.

Page 30: Examples of Viral Genomes

  • Comparative classification of various viral families and their characteristics.

Page 31: Hierarchical Virus Classification Example

  • Order: Herpesvirale | Family: Herpesviridae | Subfamily: Alphaherpes virus | Genus: Simplex virus | Species: Herpes Simplex virus (HSV).

Page 32: DNA Viruses Overview

  • Focus on DNA viruses causing human diseases and relevant examples.

Page 33: Specific DNA Viruses

  • Notable human pathogens in the DNA virus category:

    • Poxviridae: Cowpox

    • Herpesviridae: Herpes simplex, Varicella zoster (chickenpox)

    • Adenoviridae, Papovaviridae, Hepadnaviridae, Parvoviridae.

Page 34: RNA Viruses Overview

  • Insight on RNA viruses that affect humans.

Page 35: Specific RNA Viruses

  • Notable RNA viruses causing human diseases and related examples:

    • Orthomyxoviridae: Influenza A/B/C

    • Paramyxoviridae: Mumps, Measles

    • Rhabdoviridae: Rabies, etc.

Page 36: Additional RNA Viruses

  • More RNA viruses relevant to human health, emphasizing those included in vaccination programs.

Page 37: Routes of Virus Transmission

  • Types of viral transmission pathways:

    • Respiratory route – airborne (flu, Covid).

    • Direct contact (Herpes simplex virus, chickenpox).

    • Insect bites (arboviruses).

    • Sexual/injection (blood-borne viruses).

    • Faecal/oral route (enteric viruses).

Page 38: Nature of Virus Testing

  • Testing for viral infections is complex; conventional methods such as agar plates are ineffective.

  • Modern testing focuses on detecting viral DNA or proteins.

Page 39: Viral Infections Mechanism

  • Example of common cold caused by rhinovirus:

    • High virus concentration is dispersed through nasal secretions.

  • Importance of hand hygiene in transmission prevention.

Page 40: Immune Response to Infection

  • Overview of immune response upon virus infection, particularly with rhinovirus:

    • Initial innate immune response actions.

Page 41: Subsequent Immune Response

  • Discussion of adaptive immune response producing antibodies against reinfection.

Page 42: Herpes Simplex Virus

  • Causes cold sores; characteristics of transmission and latent infection.

Page 43: Varicella-Zoster Virus

  • Focus on chickenpox and shingles:

    • Dormancy and potential for future reactivation in individuals.

Page 44: Subclinical Infections

  • Clarification on human viruses:

    • Some cause subclinical or asymptomatic infections rather than overt disease.

Page 45: Lifecycle of Viruses

  • Overview of the lifecycle stages of a virus:

    • Attachment, penetration, replication, assembly, release.

Page 46: Replication of DNA Viruses

  • Description of DNA virus replication processes related to the Baltimore classification.

Page 47: Replication of RNA Viruses

  • Explanation of RNA virus replication processes aligned with the Baltimore classification.

Page 48: Importance of Virus Lifecycle Understanding

  • Key factors for viruses:

    1. Ability to enter target cells.

    2. Capacity for efficient replication.

    3. Ability to exit host cells.

Page 49: Antiviral Treatments

  • Challenges in developing antiviral treatments.

  • Increasing number of antiviral agents, particularly for specific viral groups.

Page 50: Mechanisms of Antiviral Action

  • Various mechanisms by which antiviral agents operate:

    • Inhibition of DNA synthesis with examples like acyclovir.

Page 51: Preparing for Future Pandemics

  • Emphasis on global surveillance and technological readiness for rapid vaccine development.

Page 52: Summary of Learnings

  • Key takeaways regarding viral structure and treatment, including genome types and antiviral therapy goals.

Page 53: Suggested Further Reading

  • References for deeper understanding:

    • Mims' Medical Microbiology and Immunology

    • Clinical and Diagnostic Virology guide.