Patterns of Inheritance
Lecture Objectives
Discuss Gregor Mendel’s discoveries, experiments, and laws.
Describe traditional forms of inheritance that Mendel discovered.
Describe concepts such as incomplete dominance, codominance, multiple alleles, pleiotropy, polygenic inheritance, linked genes, and sex-linked genes.
Complete monohybrid and dihybrid crosses.
Interpret a pedigree.
Mendel's Uncovering of Basic Laws of Inheritance
Gregor Mendel utilized pea plants to conduct his studies on heredity.
Reason for Choosing Pea Plants:
Pea plants presented easily observable and discrete “heritable factors” such as flower color and seed shape, which included the following traits:
Flower color: Purple, White
Seed shape: Round, Wrinkled
Seed color: Yellow, Green
Flower position: Axial, Terminal
Pod color: Green, Yellow
Pod shape: Inflated, Constricted
Stem length: Tall, Dwarf
Definitions:
Character: A heritable feature that varies among individuals.
Trait: A variant of a character.
Mendel studied characters that occurred in two distinct traits.
Structure of Flowers in Pea Plants
Carpels: Female reproductive parts (produce eggs).
Stamens: Male reproductive parts (produce pollen).
Mendel controlled self-pollination and cross-pollination to perform his experiments.
Mendel’s Experimental Procedure
Removed stamens from a purple flower and transferred pollen from stamens of a white flower to the carpel of the purple flower.
Parents in the experiments are referred to as the parental (P) generation.
Their hybrid offspring are called the F1 generation, and a cross of the F1 plants results in the F2 generation.
Monohybrid Cross
Definition: A cross between purebred parent plants that differs in only one character.
Hybrids: Offspring produced from two different purebred varieties.
Generational Labels:
P generation (parental)
F1 generation (first offspring)
F2 generation (second offspring; typically displayed in a 3:1 ratio for dominant to recessive traits)
Mendel's Principles of Inheritance
Alternative versions of genes are referred to as alleles (e.g., purple and white for flower color).
Genotype: The specific combination of alleles an organism carries.
Phenotype: The physical characteristics expressed as a result of the genotype.
An organism gets two alleles for each inherited character (one from each parent).
Organism is homozygous for a gene if both alleles are identical.
Organism is heterozygous for a gene if the alleles differ.
Dominant Allele: Determines appearance if present.
Recessive Allele: Has no noticeable effect on appearance when dominant allele is present.
Examples of Seed Color Inheritance
Yellow seed color is dominant (Y) and green seed color is recessive (y).
Genotypes and their corresponding phenotypes:
Homozygous dominant (YY) → Yellow
Heterozygous (Yy) → Yellow
Homozygous recessive (yy) → Green
Law of Segregation
States that the two alleles for a gene segregate from one another during gamete production, meaning that each gamete carries only one allele for each character.
This law is explained by the behavior of homologous chromosomes during meiosis.
Punnett Squares
Purpose: To visualize and predict the offspring genotypes and phenotypes from parental crosses.
Example: A monohybrid cross between two heterozygous parents (Yy x Yy).
Produces offspring in a 3:1 phenotype ratio (3 yellow, 1 green) and a 1:2:1 genotype ratio (1 YY : 2 Yy : 1 yy).
Test Cross
Definition: A method to determine an organism's unknown genotype by mating it with a homozygous recessive individual.
Example: A black lab (with unknown genotype) crossed with a chocolate lab (genotype bb).
Test Cross Results:
If the black lab is BB, all offspring will be black (Bb).
If the black lab is Bb, there will be a 1:1 ratio of black (Bb) to chocolate (bb) offspring.
Dihybrid Cross
Definition: A genetic cross that examines the inheritance of two traits simultaneously, demonstrating Mendel's law of independent assortment.
Gamete combinations for BbDd cross:
Possible allele combinations:
BD, Bd, bD, bd
Resulting offspring phenotypes (9:3:3:1 ratio) include:
9 Black, Hearing
3 Black, Deaf
3 Chocolate, Hearing
1 Chocolate, Deaf
Inheritance Complications
Discussion of non-Mendelian inheritance patterns:
Incomplete dominance: Heterozygotes exhibit an intermediate phenotype.
Codominance: Heterozygotes display both alleles (e.g., ABO blood types).
Multiple alleles: More than two alleles present for a trait (e.g., ABO blood groups).
Pleiotropy: A single gene affects multiple traits (e.g., sickle-cell disease).
Polygenic inheritance: Multiple genes contribute to a single phenotype (e.g., skin color, height).
Linked genes: Genes located close to each other on the same chromosome tend to be inherited together.
Environmental effects: Environmental factors can influence phenotypic expression.
ABO Blood Types
Genotypes and Phenotypes:
Type A: AA or Ai, surface molecule A.
Type B: BB or Bi, surface molecule B.
Type AB: AB, surface molecules A and B.
Type O: ii, no surface molecules.
The ABO blood types are determined by the presence of specific sugars added to the cell surface by corresponding alleles.
Pleiotropy Example - Sickle-cell Disease
One gene influences multiple phenotypes, leading to various symptoms such as weakness, pain, and organ damage due to abnormal hemoglobin.
Polygenic Inheritance and Continuous Variation
Traits like skin color and height exhibit a range of phenotypes due to the additive effects of multiple genes.
Linked Genes and Crossing Over
Genes located on the same chromosome are considered linked and do not assort independently during gamete formation.
Crossing Over: A process during meiosis that can separate linked genes, allowing for recombination of alleles.
Sex-Linked Genes
Inheritance patterns differ for sex-linked traits (located on X and Y chromosomes).
Males inherit one allele from their mother (X) and one Y chromosome.
Females need two recessive alleles for expression of X-linked recessive disorders.
Example: X-inactivation occurs in females, preventing double expression of genes from both X chromosomes.
Pedigree Analysis
Pedigrees are useful tools for tracking inheritance patterns of traits in families. Each symbol in a pedigree diagram represents individuals’ traits (affected or unaffected).
Example cases may involve determining genotypes of parents based on offspring traits and analyzing carrier status.
Chapter Summary and Review Materials
The chapter reviews Mendelian genetics, including law of segregation, law of independent assortment, and various inheritance complexities.
Refer to provided pages for summaries, multiple choice questions, and practical problem-solving strategies in genetics (pages 191-195).