Collection of Specimens: Stool, Urine, and Sputum

Urinary Analysis (UA)

  • Abbreviated as UA, it checks for bacteria in urine.
  • If bacteria are present, it indicates a urinary tract infection (UTI).
  • Other reasons for urine collection include checking sugar levels, kidney function, electrolytes (sodium, potassium), and drug levels.

Basic Principles of Collection and Transport

  • Always wear appropriate personal protective equipment (PPE) when collecting samples.
  • Determine how to collect the sample and what to store it in, following the nurse's directions.
  • Use the correct container for the specific test.
  • Verify the required amount of urine or stool.
  • Consider timing requirements (e.g., first morning void, specific intervals).
  • Assemble all supplies beforehand (container, biohazard bag, commode hat, PPE).
  • Label the container or biohazard bag with date, time, resident name, and date of birth before obtaining the sample.
  • After collection, place the sample in a biohazard bag, remove gloves, perform hand hygiene, and transport the sample to the nurse or designated storage area.
  • Refrigerate samples if needed until processed; alert the nurse upon completion.

Urine Specimens

  • Collected for various reasons; the most common is urinary analysis (UA) to detect bacteria indicating a UTI.
  • Other reasons include checking for sugar, kidney function, electrolytes, and drug levels.

Clean Catch Urine Sample

  • Used to check for a UTI.
  • The resident must be able to sit on the toilet or commode.
  • If the resident cannot sit, the nurse must perform straight catheterization.
  • Never obtain a clean catch urine sample from a bedpan, urinal, or commode to avoid contamination.
  • Contaminated samples would reflect bacteria in the collection item, not the urine.

Procedure to avoid contamination:

  • Clean the resident's peri-area with an antiseptic towelette in a front-to-back motion, wiping from the urethra toward the anus three times.
  • Some containers include povidone-iodine swabs instead of towelettes.
  • Ask the resident to start and stop voiding.
  • Place the cup under the urethra without touching the body.
  • Instruct the resident to continue voiding and fill the cup to the level indicated by the nurse (usually at least 50 mL).
  • If the resident cannot start and stop, collect after voiding has started and remove before voiding stops.
  • Place the lid without touching the inside, and place the container in a biohazard bag.
  • Attend to the resident's needs and transport the sample to the nurse or designated storage area.

Straining for Kidney Stones

  • Required for residents with kidney stones.
  • Collect all urine using a commode hat placed in the front half of the toilet or commode under the seat.
  • Remind the resident to discard used toilet paper in the wastebasket.
  • After voiding, empty the commode hat contents through a strainer into the toilet.
  • Inspect the strainer for stones (which may be small).
  • Place any found stones in a labeled container, seal the lid, and place it in a biohazard bag.
  • Give the sample to the nurse or place it in the designated storage area.

Fecal Specimens

  • Necessary for residents with digestive problems or diarrheal illnesses.
  • Stool is not sterile, so samples can be collected from commodes, commode hats, and bedpans if they are new and unused.
  • Avoid urine contamination.
  • If assisting a male, have him void in a urinal first; for a female, have her empty her bladder first.
  • Use a commode hat or bedpan to collect the stool.
  • Follow the nurse's instructions on the number of samples needed.
  • Place the commode hat under the seat in the back half of the toilet or commode.
  • Instruct the resident to put used toilet paper in the wastebasket.
  • After defecation, collect the sample using a wooden tongue blade or plastic spoon from three different areas (ends and middle).
  • Fill the specimen cup as directed by the nurse, seal the lid, and place it in a biohazard bag.
  • Give the sample to the nurse or place it in the designated storage area.

Special containers for infectious diarrheal illnesses:

  • Small tubes with screw tops and a spoon attached to the lid.
  • Scoop the stool into the container, mix it with the fluid inside, and fill to the indicated line.
  • Seal the lid and place the container in a biohazard bag.
  • Give it to the nurse or place it in the designated storage area.

Occult Blood

  • Occult blood is "hidden" blood in the stool.
  • Tests include stool guaiac, fecal occult blood test (FOBT), Hemosure, and Hemoccult.
  • The nursing assistant may or may not be permitted to perform this test based on facility protocol.

Procedure:

  • After bowel movement in a bedpan, commode pan, or commode hat, collect a small stool sample with the provided stick or tongue blade.
  • Wipe the stool on one area of the Hemoccult card under window A, and close the flap.
  • Take a second sample from a different area and wipe it under window B, and close the flap.
  • Turn the card over and drop the prescribed amount of developer over the front window.
  • Follow the manufacturer's directions, as tests may vary.
  • A blue color usually indicates a positive result for occult blood.
  • Place the card in a biohazard bag and give it to the nurse for further assessment.
  • If frank or occult blood is observed during toileting, do not flush the toilet and inform the nurse; a large amount of frank blood or visible rectal bleeding is a medical emergency.

Sputum Specimens

  • Required for residents with respiratory illnesses to identify the proper treatment.
  • Sputum is mucous expelled from the lungs, which is colored and thick (unlike saliva, which is clear and thin).
  • The nursing assistant reinforces instructions from the nurse and provides a sterile container.
  • Remind the resident to deposit sputum (not saliva) into the container when coughing and to call the nursing assistant to collect the container after obtaining the specimen.
  • Follow the principles of collection, transport, and infection control.