Collection of Specimens: Stool, Urine, and Sputum
Urinary Analysis (UA)
- Abbreviated as UA, it checks for bacteria in urine.
- If bacteria are present, it indicates a urinary tract infection (UTI).
- Other reasons for urine collection include checking sugar levels, kidney function, electrolytes (sodium, potassium), and drug levels.
Basic Principles of Collection and Transport
- Always wear appropriate personal protective equipment (PPE) when collecting samples.
- Determine how to collect the sample and what to store it in, following the nurse's directions.
- Use the correct container for the specific test.
- Verify the required amount of urine or stool.
- Consider timing requirements (e.g., first morning void, specific intervals).
- Assemble all supplies beforehand (container, biohazard bag, commode hat, PPE).
- Label the container or biohazard bag with date, time, resident name, and date of birth before obtaining the sample.
- After collection, place the sample in a biohazard bag, remove gloves, perform hand hygiene, and transport the sample to the nurse or designated storage area.
- Refrigerate samples if needed until processed; alert the nurse upon completion.
Urine Specimens
- Collected for various reasons; the most common is urinary analysis (UA) to detect bacteria indicating a UTI.
- Other reasons include checking for sugar, kidney function, electrolytes, and drug levels.
Clean Catch Urine Sample
- Used to check for a UTI.
- The resident must be able to sit on the toilet or commode.
- If the resident cannot sit, the nurse must perform straight catheterization.
- Never obtain a clean catch urine sample from a bedpan, urinal, or commode to avoid contamination.
- Contaminated samples would reflect bacteria in the collection item, not the urine.
Procedure to avoid contamination:
- Clean the resident's peri-area with an antiseptic towelette in a front-to-back motion, wiping from the urethra toward the anus three times.
- Some containers include povidone-iodine swabs instead of towelettes.
- Ask the resident to start and stop voiding.
- Place the cup under the urethra without touching the body.
- Instruct the resident to continue voiding and fill the cup to the level indicated by the nurse (usually at least 50 mL).
- If the resident cannot start and stop, collect after voiding has started and remove before voiding stops.
- Place the lid without touching the inside, and place the container in a biohazard bag.
- Attend to the resident's needs and transport the sample to the nurse or designated storage area.
Straining for Kidney Stones
- Required for residents with kidney stones.
- Collect all urine using a commode hat placed in the front half of the toilet or commode under the seat.
- Remind the resident to discard used toilet paper in the wastebasket.
- After voiding, empty the commode hat contents through a strainer into the toilet.
- Inspect the strainer for stones (which may be small).
- Place any found stones in a labeled container, seal the lid, and place it in a biohazard bag.
- Give the sample to the nurse or place it in the designated storage area.
Fecal Specimens
- Necessary for residents with digestive problems or diarrheal illnesses.
- Stool is not sterile, so samples can be collected from commodes, commode hats, and bedpans if they are new and unused.
- Avoid urine contamination.
- If assisting a male, have him void in a urinal first; for a female, have her empty her bladder first.
- Use a commode hat or bedpan to collect the stool.
- Follow the nurse's instructions on the number of samples needed.
- Place the commode hat under the seat in the back half of the toilet or commode.
- Instruct the resident to put used toilet paper in the wastebasket.
- After defecation, collect the sample using a wooden tongue blade or plastic spoon from three different areas (ends and middle).
- Fill the specimen cup as directed by the nurse, seal the lid, and place it in a biohazard bag.
- Give the sample to the nurse or place it in the designated storage area.
Special containers for infectious diarrheal illnesses:
- Small tubes with screw tops and a spoon attached to the lid.
- Scoop the stool into the container, mix it with the fluid inside, and fill to the indicated line.
- Seal the lid and place the container in a biohazard bag.
- Give it to the nurse or place it in the designated storage area.
Occult Blood
- Occult blood is "hidden" blood in the stool.
- Tests include stool guaiac, fecal occult blood test (FOBT), Hemosure, and Hemoccult.
- The nursing assistant may or may not be permitted to perform this test based on facility protocol.
Procedure:
- After bowel movement in a bedpan, commode pan, or commode hat, collect a small stool sample with the provided stick or tongue blade.
- Wipe the stool on one area of the Hemoccult card under window A, and close the flap.
- Take a second sample from a different area and wipe it under window B, and close the flap.
- Turn the card over and drop the prescribed amount of developer over the front window.
- Follow the manufacturer's directions, as tests may vary.
- A blue color usually indicates a positive result for occult blood.
- Place the card in a biohazard bag and give it to the nurse for further assessment.
- If frank or occult blood is observed during toileting, do not flush the toilet and inform the nurse; a large amount of frank blood or visible rectal bleeding is a medical emergency.
Sputum Specimens
- Required for residents with respiratory illnesses to identify the proper treatment.
- Sputum is mucous expelled from the lungs, which is colored and thick (unlike saliva, which is clear and thin).
- The nursing assistant reinforces instructions from the nurse and provides a sterile container.
- Remind the resident to deposit sputum (not saliva) into the container when coughing and to call the nursing assistant to collect the container after obtaining the specimen.
- Follow the principles of collection, transport, and infection control.