Biological molecules
Body mass
65% of the body’s mass is oxygen
18% of the body’s mass is carbon
10% of the body’s mass is hydrogen
Carbohydrates
C,H,O arranged in a ring shape = simple sugar
Glucose is of simple sugar and is a monosaccharide
Disaccharide = simple sugars joined in a pair, e.g. sucrose
Polysaccharide = Large molecules of simple sugars, e.g. starch, glycogen and cellulose
Proteins
Consist of long chains of amino acids
20 different amino acids joined in any order in animal and plant cells
There are structural proteins that form muscle, enzymes and some hormones
Lipids
Made of fatty acids and glycerol
Contain 3 fatty acids and 1 glycerol
Fats = solid at room temperature, oils = liquid at room temperature
They form cell membranes, steroid hormones and fats in storage cells
Tests
Starch
Drop a few drops of iodine / potassium iodide solution onto food on spotting tile or mix with solution
Brown → black-blue
Glucose
Add equal volume of pale blue Benedict’s solution to the sample and heat to 95C
Blue → Orange-red (considerable glucose), Yellow (moderate), Green (small)
Protein
Add 1cm3 of Biurettes solution to food sample
Blue → Purple
Lipid
Perform emulsion test
Add 4cm3 of ethanol to food sample, shake well and leave for one minute for solution to settle
Remove top part of filtrate and add to to test tube of water
Clear → Cloudy solution or layer