Comprehensive Study Notes on Forensic Sciences: Personal Identification, Photography, Chemistry & Toxicology, Questioned Documents, and Polygraphy
Personal Identification Techniques
Definition of Identification:
- Determination of the individuality of a person or thing.
- Recognizing characteristics of persons to differentiate them from others.
Methods of Personal Identification:
- Layman Identification: Based on simple means without scientific methods or special training (e.g., clothing, body ornaments, general height/weight, hair color).
- Scientific Identification: Made by trained experts using specialized knowledge, skill, observation, comparison, and exclusion (e.g., Dactyloscopy, Forensic Anthropology, Forensic Odontology, DNA Profiling).
Laws & Rules Governing Personal Identification:
- Law of Multiplicity of Evidence in Identification: The greater the number of points of similarities and dissimilarities between two persons compared, the greater the probability for the conclusion to be correct.
- Value of points of identification varies in formulating a conclusion.
- The longer the interval between death and examination of remains, the greater the need for experts in establishing identity.
- In cases of mass disasters, immediate action is required because biological evidence is highly perishable.
- There is no rigid rule to be observed in the procedure of personal identification.
Bases of Human Identification:
- By Comparison: Comparing criteria recovered during investigation with records in file (e.g., ante-mortem records vs. post-mortem findings).
- By Exclusion: Process of elimination when identifying multiple individuals.
Biometric Identification Modalities:
- Fingerprint Identification: Analysis of friction ridge patterns.
- Iris Recognition: Analyzing patterns of the eye iris.
- Facial Recognition: Mapping facial feature ratios and geometry.
- Voice Recognition: Acoustic and phonetic speech pattern analysis.
Gait Analysis (Manner of Walking):
- Ataxic Gait: Foot is raised high, thrown forward, and brought down suddenly; seen in tabes dorsalis.
- Cerebellar Gait: Staggering movement seen in cerebellar diseases.
- Cow's Gait: Swaying movement due to knock-knees.
- Paretic Gait: Steps are short, feet dragged, and legs held widely apart.
- Spastic Gait: Legs held together, move stiffly, and toes dragged.
- Festinating Gait: Involuntary movement in short, accelerating steps.
- Frog Gait: Hopping gait resulting from infantile paralysis.
- Waddling Gait: Exaggerated lateral trunk movement similar to a duck.
Points of Identification for Living Persons:
- Easily Changed: Hair/beard/mustache growth, clothing, frequent place of visit, profession, body ornamentations, tattoos, scarification.
- Not Easily Changed: Mental memory, speech, gait, mannerisms (stereotyped habits), complexion, eye changes, left- or right-handedness.
- Skin Layers: Temporary outer layer is the Epidermis; permanent inner layer is the Dermis. Tattoos and permanent scars must penetrate into the dermis.
Facial Expressions (Facies) Caused by Disease/Racial Influence:
- Hippocratic Facies: Pinched nose, hollow temples, sunken eyes, cold ears, relaxed lips, livid skin; indicative of approaching death.
- Mongolian Facies: Almond eyes, pale complexion, prominent cheekbones.

- Facies Leonine: Deeply furrowed, lion-like face observed in leprosy, elephantiasis, and leontiasis ossia.

- Myxedemic Facies: Pale face, edematous non-pitting swelling, dry coarse sparse hair, thin lateral eyebrows, periorbital edema, dull intellect, slow monotonous speech, muscular weakness, and tremor.
Forensic Anthropology & Forensic Odontology:
- Forensic Anthropology: Examination of skeletal remains to recover human remains, establish identity, interpret trauma, and estimate time since death.
- Forensic Odontology: Application of dental science to legal investigations (identifying offenders via bite marks or victims via ante-mortem dental records). Tooth enamel is the hardest substance in the human body.
- Presidential Decree 1575 (PD 1575): Requires dental practitioners to keep records of their patients for at least from the last entry.
- Permanent Teeth Eruption Timeline:
- : Lower/Upper First Molars, Lower Central Incisors
- : Upper Central Incisors, Lower Lateral Incisors
- : Upper Lateral Incisors
- : Lower Canines
- : Upper First Premolars
- : Lower First Premolar, Upper Second Premolar
- : Lower Second Premolar, Upper Canine
- : Lower Second Molar
- : Upper Second Molar
- : Third Molars (Wisdom Teeth)
DNA (Deoxyribonucleic Acid) Profiling:
- Determines unique DNA characteristics (DNA fingerprinting).
- Any two random humans share approximately of their DNA. The variation accounts for all individual differences (equals approximately base differences).
- DNA Similarity Across Relatives:
- Identical Twins:
- Parent & Child:
- Full Siblings: average
- Grandparent & Grandchild:
- First Cousins:
- Short Tandem Repeats (STR): Analysis measuring repeating sequences () at specific loci on chromosomes. Utilizes Restriction Endonucleases and Gel Electrophoresis on agarose.
- Paternity testing relies on the principle that a child inherits half of their chromosomes from each biological parent.
Determination of Sex & Age:
- Sex Determination:
- Tests: Social, Genital, Gonadal, Chromosomal (; Male = , Female = , Superfemale = ).
- Conclusive Evidence: Presence of ovaries in females and testes in males.
- Age Determination Indicators:
- Pubic Hair: (Female), (Male).
- Mustache & Beard: (Male).
- Breast Changes: (Female).
- Voice Development: (Both).
- Hair Color Changes: Graying at , pubic hair graying at .
- Menstruation: (Warm countries).
- Sex Determination:
Light as a Factor in Distance Recognition:
- Clearest Moonlight: Maximum distance of .
- Starlight: Maximum distance of .
- Broad Daylight: Unknown persons at ; near strangers at .
History & Principles of Dactyloscopy:
- Dactylography: Scientific study of fingerprints as a means of identification.
- Dactyloscopy: Practical application of fingerprint science (classification and identification).
- Dactylomancy: Fingerprint study for personality interpretation.
- Dermatoglyphics: Study of skin patterns (Derma = skin, Glype = carve).
- Leading Judicial Cases:
- People vs. Jennings (1911, USA): First judicial recognition of fingerprint evidence for conviction.
- People vs. Medina (1933, Philippines): First conviction based on fingerprint evidence in Philippine jurisprudence.
- Three Dogmatic Principles of Fingerprints:
- Individuality / Variation / Uniqueness: No two persons have identical fingerprints. Sir Francis Galton (1892) calculated the probability of two identical fingerprints as .
- Constancy / Persistency / Permanency / Immutability: Friction ridges remain unchanging from birth until post-mortem decomposition ("From Womb to Tomb"). Ridges form during the of fetal life and complete by the .
- Infallibility: Fingerprints cannot be forged; universally accepted as a reliable mean of identification.
- Historical Ridge Destruction Attempts:
- John Dillinger: Tried erasing prints with acid; prints regenerated.
- Robert Roscoe James Pitts ("Man without fingerprints"): Surgically removed tip friction skin and grafted chest skin; identified via remaining ridge characteristics and palm prints.
Anatomy of Friction Skin & Ridge Characteristics:
- Skeletal Finger (Phalanges): Basal/Proximal (nearest palm), Middle/Intermediate, Terminal/Distal (tip bone covered with friction skin).
- Friction Skin Components: Elevated Ridges (hills, appear black in inked prints) and depressed Furrows (valleys, white lines).
- Glands: Eccrine (soles and palms only), Apocrine (pubic/mammary/anal), Sebaceous (forehead, chest, back - secretes oils).
- Dermal Papillae: Irregular pegs protruding into the epidermis. Permanent scarring (Cicatriz) occurs if dermal damage exceeds depth.
- Congenital Anomalies:
- Polydactyl: Extra fingers.
- Macrodactyl: Enlarged fingers.
- Brachydactyl: Abnormally short fingers.
- Ectodactyl: Missing fingers at birth.
- Syndactyl: Webbed fingers / side fusion.

- Ankylosis: Inability to bend finger joints.
- Orthodactyl: Inability to stretch fingers.
Minutiae / Galton Details:
- Basic Types: Bifurcation, Ending Ridge, Ridge Dot (Island).
- Other Details: Converging, Diverging, Enclosure (Lake), Trifurcation, Opposed Bifurcation, Spur (Hook), Puckering Ridge, Appendage (Abutment), Crossover (Bridge), Fragment (), Envelope, Incipient/Nascent ridges (thin/underdeveloped, not counted), Dissociated ridges (patchy, not counted).

Focal Points & Pattern Types:
- Pattern Area: Part of loop or whorl enclosed by typelines containing delta and core.
- Typelines: Innermost parallel ridges that diverge and surround the pattern area.
- Delta (Outer Terminus): First point on ridge formation at or directly in front of typeline divergence.
- Core (Inner Terminus): Focal point in the center of the innermost recurve.
- General Pattern Families:
- Loops ( total frequency): Ulnar Loop ( of loops), Radial Loop ( of loops). Requirements: Delta, Core, Recurving ridge between delta/core, Ridge count .
- Whorls ( total frequency): Plain Whorl (), Central Pocket Loop Whorl (), Double Loop Whorl (), Accidental Whorl ().
- Arches ( total frequency): Plain Arch ( of arches), Tented Arch ( of arches - Angle, Upthrust, Incomplete Loop types).

Henry-FBI Fingerprint Classification Formula:
- Order of Classification Divisions: Key - Major - Primary - Secondary - Sub-Secondary - Final.
- Primary Classification:
- Assign numerical values only to Whorl patterns ():
- Fingers 1 & 2 (Right Thumb, Right Index) =
- Fingers 3 & 4 (Right Middle, Right Ring) =
- Fingers 5 & 6 (Right Little, Left Thumb) =
- Fingers 7 & 8 (Left Index, Left Middle) =
- Fingers 9 & 10 (Left Ring, Left Little) =
- Arches () and Loops () =

- Formula:
- Assign numerical values only to Whorl patterns ():
- Secondary Classification:
- Capital Letter Series () for Index Fingers (Right = numerator, Left = denominator).
- Small Letter Series () for Thumbs, Middle, Ring, Little fingers placed around index letters.
- Sub-Secondary Classification:
- Examines Index, Middle, and Ring fingers.
- Loops (Ridge Counting):
- Index: Inner () = ; Outer () =
- Middle: Inner () = ; Outer () =
- Ring: Inner () = ; Outer () =
- Whorls (Ridge Tracing): Inner () = ridges inside/above right delta; Outer () = ridges outside/below right delta; Meeting () = ridges difference.
- Arches: Represented by a dash ().
- Major Classification:
- Examines Thumbs (Right = numerator, Left = denominator).
- If Left Thumb Loop count : Small () = ; Medium () = ; Large () = .
- If Left Thumb Loop count : Right Thumb scale shifts , , .
- Whorls: Evaluated via Ridge Tracing ().
- Final Classification:
- Ridge count of loops or whorls on Little Fingers (Finger 5 = numerator, Finger 10 = denominator).
- Key Classification:
- Ridge count of the first Loop appearing in a set of prints, excluding the little fingers.
- Amputations & FMB (Finger Missing at Birth):
- Single amputation: Classify identical to opposite finger.
- Both opposite fingers amputated: Given Whorl classification with Meeting () tracing.
- All 10 fingers missing: .
Latent Print Processing & Examination:
- Types of Chance Prints: Patent/Visible (ink, blood, grease), Plastic/Semi-visible (3D soft impressions in wax, soap), Latent/Invisible (sweat/oil deposits).
- Development Methods: Dusting/Powder, Rolling, Fuming (Iodine/Ammonia), Silver Nitrate ( solution), Ninhydrin, Laser.
- ACE-V Methodology: Analysis, Comparison, Evaluation, Verification.
Forensic Photography
Etymology & Definitions:
- Derived from Greek: Phos ("light") and Graphos ("write") "Write with light".
- Police Photography: Art or science of studying photography principles, preparing photographic evidence, and applying it to police work.
- Forensic Photography: Art or science of photographically documenting a crime scene and evidence for laboratory analysis and court trials.
- Utmost Use: Record purposes.
- First Police Application: Personal Identification introduced by Alphonse Bertillon (Anthropometry System mugshots).
Primary Functions & Crime Scene Camera Views:
- 4 Functions: Identification; Discovering, recording, and preserving; Presenting court exhibits; Training and public relations.
- Camera Views:
- General / Long-Range View: Overall coverage showing location, direction, and surroundings.
- Medium View: Divides scene into sections; shows 4 angles, entrances, exits, and nature of the crime.
- Close-Up View: Individual photographs of physical evidence detailing specific characteristics.
- Extreme Close-Up View: High-magnification photographs (Photomacrography or Photomicrography) showing minute details/damage.
Essentials & Physics of Light:
- Speed of Light: Approximately ( or in a vacuum).
- Wavelength (): Measured in Angstroms, Nanometers (), or Millimicrons (). Measured crest-to-crest.
- Light Theories:
- Wave Theory (Christian Huygens): Light travels in continuous wave motions.
- Corpuscular Theory (Isaac Newton): Light consists of tiny emitted particles (electrons).
- Electromagnetic Theory (Maxwell & Hertz): Light is electromagnetic radiation waves.
- Quantum Theory (Albert Einstein): Light energy packet emissions (photons).
Electromagnetic Spectrum Divisions:
- Gamma & Cosmic Rays: Shortest wavelengths, highest photon energy.
- X-Ray: ; discovered by Wilhelm Conrad Roentgen; shadow photography principle.
- Ultra-Violet (UV) Rays: ; "Black Light"; used for invisible ink, altered documents, bodily fluids, multi-colored background fingerprints.
- Visible Light: ( Violet to Red).
- Infra-Red (IR) Rays: ; heat rays; used for obliterated/charred documents, gunshot residue on dark cloth, black-out photography.
Color Theory of Light:
- Additive Primary Colors: Blue, Green, Red. Additive Combinations: Red + Green = Yellow; Red + Blue = Magenta; Green + Blue = Cyan; Red + Green + Blue = White.
- Subtractive Primaries: Cyan, Yellow, Magenta. Subtractive Combinations: White - Blue = Yellow; White - Red = Cyan; White - Green = Magenta.
Light Interactions & Mediums:
- Mediums: Transparent (transmits light), Translucent (transmits light), Opaque (absorbs/reflects light).
- Interactions (RAT): Reflected, Absorbed, Transmitted.
- Bending Modes:
- Reflection: Rebounding of light rays.
- Refraction: Bending of light passing from one medium to another.
- Diffraction: Bending of light rays striking the edge of an opaque barrier.
Sources of Light:
- Natural Sunlight: Classified by intensity: Bright Sunlight (deep uniform shadows), Hazy Sunlight (bluish transparent shadows), Dull Sunlight (no shadows, clouds cover sun), Cloudy Bright, Cloudy Dull.
- Artificial Lights:
- Continuous: Photoflood lamps (), Fluorescent, Incandescent, IR/UV lamps.
- Short Duration: Flashbulbs (single-use chemical combustion), Electronic Flash ( speed; Strobe light, Slave unit, Ring light [encircles lens], On-camera, Off-camera, Fill-in, Bounce, Umbrella).
Sensitized Materials & Chemical Processing:
- Film Structure:
- Black & White Film: Scratch-resistant top coating, Emulsion layer (Silver Halides suspended in Gelatin), Film Base (cellulose acetate), Antihalation Backing (black dye absorbing penetrating light to prevent halo double images).
- Color Film: 3 Emulsion layers (Top: Blue-sensitive; Yellow Filter/Carey Lea silver; Middle: Green-sensitive/Orthochromatic; Bottom: Red-sensitive/Panchromatic), Base, Antihalation backing.
- Film Speed Systems:
- ASA: Arithmetic scale ( is faster than ).
- DIN: Logarithmic scale ( doubles sensitivity).
- ISO: Combined scale (e.g., ISO , ISO ).
- Photographic Paper Layers: Emulsion, Baryta layer (increases reflectivity), Base (Lightweight, Single Weight, Double Weight).
- Paper Grades (Velox): No. 0 (extremely high contrast negatives), No. 1 (high contrast), No. 2 (normal), No. 3 (weak contrast), No. 4 (thin/weak negatives), No. 5 (extremely flat negatives).
- Chemical Processing Sequence:
- Development: Converts exposed silver halides to metallic silver.
- Developers: D-76 (films), Dektol (papers).
- Components: Hydroquinone & Elon (reducers), Sodium Sulphite (preservative), Sodium Carbonate (accelerator), Potassium Bromide (restrainer/antifoggent).
- Stop-Bath: Halts development (Water + Acetic Acid).
- Fixing: Removes unexposed silver halides to make image permanent.
- Components: Sodium Thiosulfate / Hypo (dissolving agent), Acetic/Boric Acid (neutralizer), Sodium Sulphate (preservative), Potassium Alum (hardener).
- Washing & Drying: Removes residual chemicals.
- Development: Converts exposed silver halides to metallic silver.
- Film Structure:
Camera Elements & Optics:
- Essential Parts: Light-tight box, Lens, Shutter, Film/Sensor holder, Viewfinder, Diaphragm/Aperture.
- Lens Types:
- Convex / Converging / Positive: Thick center, converges light; corrects hypermetropia (farsightedness).
- Concave / Diverging / Negative: Thin center, diverges light; corrects myopia (nearsightedness).
- Focal Length Classification:
- Wide-Angle: Focal length diagonal of sensor/film; wide area coverage.
- Normal Lens: Focal length diagonal of sensor/film; matches human eye view.
- Telephoto Lens: Focal length diagonal of sensor/film; narrow view, magnifies distant objects.
- Zoom Lens: Variable focal length.
- Lens Aberrations / Defects:
- Spherical Aberration: Peripheral light rays focus closer to lens than central rays.
- Coma: Off-axis point light sources appear comet-shaped (Positive = tail pointing toward axis; Negative = away).
- Curvature of Field (Petzval): Plane image projected onto a curved focal plane.
- Distortion: Shape defect (Barrel distortion in wide-angle lenses; Pincushion distortion in telephoto lenses).
- Chromatic Aberration: Dispersion causing different wavelengths/colors to focus at different points.
- Astigmatism: Inability to focus horizontal and vertical lines simultaneously.
- Lens Correction Classifications:
- Meniscus: Uncorrected lens.
- Achromatic: Corrects chromatic and spherical aberrations.
- Rapid Rectilinear: Corrects distortion.
- Anastigmat: Corrects spherical aberration, coma, and astigmatism.
- Apochromat: Corrects chromatic aberration and astigmatism across three spectral colors.
Exposure Variables:
- Shutter Speed: Regulates time exposure (; Bulb [], Time []).
- Aperture / -number: Ratio of focal length to effective lens aperture diameter (). Smaller -number = larger aperture opening = greater light volume.
- Depth of Field (DOF): Distance between nearest and farthest objects in sharp focus. Increased by smaller aperture (larger -number), shorter focal length, or greater subject distance.
Admissibility of Photographic Evidence:
- Best Evidence Rule: Photograph is a secondary representation, not a legal replacement for the physical object itself unless the physical evidence cannot be brought to court (e.g., roads, healing wounds).
- Admissibility Requirements: Faithful representation (accurate likeness), Relevance, Materiality, Competency, Non-inflammatory/unbiased.
Forensic Chemistry and Toxicology
Definitions & Scope:
- Forensic Chemistry: Application of chemical principles and theories in solving legal problems.
- Republic Act No. 754 (RA 754): The Chemistry Law enacted June 18, 1952.
- Golden Rules in Forensic Chemistry:
- Go slowly (take required time).
- Be thorough (careful observation).
- Take notes (utilize logbooks, photos).
- Consult others (review similar cases).
- Use imagination.
- Avoid complicated theories (explain in layman terms).
Historical Pioneers:
- Dr. Edmond Locard: Father of modern forensics; established 1st crime lab in Lyon, France (1910). Formulated Locard's Exchange Principle: "Every contact leaves a trace" / "Whenever two objects come into contact, there is a transfer of material."
- Mathieu Orfila: Father of Modern Forensic Toxicology. Used the Marsh Test to detect arsenic in the body of Charles LaFarge, convicting Marie LaFarge.
Evidence Collection & Chain of Custody:
- Liquid Items: Transported in leak-proof, non-breakable containers.
- Arson Evidence: Air-tight metal canisters.
- Dry Powders: Plastic bags.
- Moist / Wet Evidence: Packaged temporarily in plastic () then air-dried to prevent mold.
- Chain of Custody (Section 21, RA 9165): Documented unbroken record of all individuals holding custody of evidence.
- Sequence: Crime Scene Evidence Evidence Collector $ ightarrow$ Forensic Examiner $ ightarrow$ Evidence Custodian $ ightarrow$ Court Presentation.
Dangerous Drugs (RA 9165 - Comprehensive Dangerous Drugs Act of 2002):
- Classifications by Effect:
- Stimulants (Uppers): Amphetamines, Caffeine, Nicotine, Methamphetamine Hydrochloride (Shabu, "Poor man's cocaine" - white odorless crystalline powder with a bitter taste).
- Depressants (Downers): Tranquilizers, Alcohol (Ethanol), Sedatives/Hypnotics, Inhalants (volatile solvents/aerosols).
- Hallucinogens (Psychedelics):
- Marijuana (Cannabis sativa / Indian Hemp): Active component THC. Hashish is concentrated resin stronger than commercial marijuana.
- LSD (Lysergic Acid Diethylamide): "Acid"; discovered by Dr. Albert Hofmann and Dr. Arthur Stoll; extremely potent.
- Ecstasy / Molly (MDMA - Methylenedioxymethamphetamine): Alters mood and perception; lasts .
- Routes of Administration: Oral, Injection (Subcutaneous / "skin popping", Intramuscular, Intravenous / IV - most rapid/efficient), Snorting, Inhalation, Buccal, Suppositories, Topical, Iontophoresis (electric current).
- Screening / Field Color Tests:
- Opium Marquis Test Purple / Violet
- Heroin Nitric Acid Test Yellow-Green
- Morphine Nitric Acid Test Red-Orange
- Cocaine Cobalt Thiocyanate Test Blue
- Barbiturates Dille-Kopanyi / Zwikker Test Violet / Blue
- Amphetamines / Shabu Marquis Test Red / Orange-Brown; Simon's Test Purple
- LSD PABA / Van Urk Test Purple
- Marijuana Duquenois-Levine (KN) Test Red Bottom Layer
- Confirmatory Tests: Gas Chromatography (GC), Thin Layer Chromatography (TLC), High-Performance Liquid Chromatography (HPLC), Mass Spectrometry, Fourier-Transform Infrared Spectroscopy (FTIR).
- Classifications by Effect:
Blood & Serology:
- Composition: Total volume (). Normal pH = .
- Formed Elements ():
- RBC (Erythrocytes): ; contains hemoglobin to carry oxygen; non-nucleated biconcave discs in mammals.
- WBC (Leukocytes): ; bodily defense.
- Platelets (Thrombocytes): Clotting function.
- Plasma (): water, solids (proteins: Albumins [most plentiful], Globulins [transports fats, antibodies], Fibrinogen [clotting]). Serum is yellow liquid expressed after blood clots.
- Formed Elements ():
- Preservation: Sodium fluoride ( room temp, indefinitely at refrigeration).
- Preliminary Color Tests for Blood:
- Benzidine / Adler Test: Reagent: Benzidine + Glacial Acetic Acid + . Positive: Intense Blue. Sensitivity: .
- Phenolphthalein / Kastle-Meyer Test: Positive: Deep Pink / Permanganate. Sensitivity: .
- Guaiacum / Van Deen Test: Positive: Blue. Sensitivity: .
- Leucomalachite Green Test: Positive: Peacock Blue / Green.
- Luminol Test: Produces Luminescence/light in dark; sensitivity ; destroys blood factors for further typing.
- Confirmatory Microcrystalline Tests:
- Takayama / Hemochromogen Test: Reagent: Pyridine + Blood Salmon-pink crystals.
- Teichmann / Hemin Test: Reagent: Glacial Acetic Acid + NaCl $ ightarrow$ Dark brownish crystals.
- Acetone-Haemin (Wagenhaar) Test: Acetone + Oxalic/Acetic acid $ ightarrow$ Dark dichroic acicular crystals.
- Biological & Grouping Tests:
- Precipitin Test: Determines human vs. non-human animal origin (forms white cloudy ring at contact interface).
- ABO Blood Grouping: Karl Landsteiner system. Antigens/Agglutinogens () on RBCs; Antibodies/Agglutinins (Anti-, Anti-) in serum. Universal donor = Group O; Universal recipient = Group AB.
- Composition: Total volume (). Normal pH = .
Bloodstain Pattern Analysis (BSPA):
- Passive Stains: Gravity action (Drips, Drops, Pools).
- Transfer Stains: Contact wipes, swipes, smears.
- Impact / Spatter Stains: Gunshot spatter (fine mist forward, larger backspatter), Cast-off (arc trajectory), Arterial spray (pulsating spurts), Expirated spatter (mixed with air/mucus).
Semen & Sex Offense Examination:
- Composition: per ejaculation; contains . Sperm length = .
- Conditions: Aspermia (absence of sperm), Oligospermia (low sperm count).
- Tests:
- Physical: UV Light $ ightarrow$ Bluish fluorescence.
- Florence Test: Reagent: Iodine + Potassium Iodide $ ightarrow$ Dark brown rosette/rhombic crystals (tests for choline).
- Barberio's Test: Reagent: Picric Acid $ ightarrow$ Yellow needle-shaped crystals (tests for spermine).
- Acid Phosphatase Test: Citrate buffer + Sodium alpha-naphthanil phosphate + Diazonium salt $ ightarrow$ Deep Purple color (conclusive).
- Microscopic Test: Verifies complete spermatozoa.
Gunpowder, Wound Ballistics, & Explosives:
- Powder Types: Black Powder ( Potassium Nitrate, Sulfur, Charcoal); Smokeless Powder (Single Base: Nitrocellulose; Double Base: Nitrocellulose + Nitroglycerin; Triple Base: + Nitroguanidine).
- Gunshot Wounds (GSW):
- Contact: Muzzle pressed against body.
- Close Discharge (): Smudging (, soot deposit) and Tattooing (up to , peppered unburned powder granules).
- Distance Discharge: .
- Entrance vs. Exit Wounds: Entrance wounds are smaller than missile, inverted edges, possess contusion collar; Exit wounds are larger, everted edges, irregular shape.
- Nitrate Detection: Paraffin Test / Diphenylamine (DPA) Test / Teodoro Gonzalez Test $ ightarrow$ Blue specks.
- Explosives: Low/Propellants (Black/smokeless powder), Primary/Initiators (Mercury fulminate, Lead azide), High Explosives (TNT, Dynamite, Nitroglycerin, RDX).
Hair, Textile Fibers, & Glass:
- Hair Anatomy: Root, Shaft, Tip. Shaft layers: Cuticle (outer overlapping scales), Cortex (thick intermediate layer, contains melanin), Medulla (central canal).
- Medullary Index (M.I.): . Human M.I. (typically ); Animal M.I. .
- Fibers: Natural (Vegetable: Cotton, Flax, Hemp, Abaca, Jute, Linen; Animal: Wool, Silk) vs. Synthetic. Differentiated via Ignition Test, Nitric acid, Picric acid, Millon's reagent, and NaOH.
- Glass Fractures:
- Radial Fractures: Radiate outward like spokes from impact point; start on the rear/opposite surface.
- Concentric Fractures: Circular arcs connecting radial cracks; start on the front/struck surface.
- 3Rs Rule: Radial cracks form Right angles on the Rear side of impact.
- RFC Rule: Radial cracks form Front angles on Concentric cracks.
Casting, Toolmarks, Metallurgy, & Toxicology:
- Plaster of Paris Casting: Add table salt to hasten setting; add borax to retard; add sodium carbonate to harden.
- Tool Impressions: Compression marks (single impact) vs. Friction/Striation marks (scratches/sliding).
- Metallurgy: Cast coins (molded) vs. Struck coins (stamped with steel dies). Serial numbers restored via chemical Etching Fluid.
- Toxicology & Poisons:
- Poisons: Arsenic (vomiting, bloody diarrhea), Cyanide (internal asphyxiation), Carbon Monoxide ("silent killer"), Hydrogen Sulfide (rotten egg odor).
- Antidotes: Mechanical (coats/absorbs), Chemical (neutralizes), Physiological (antagonists).
- Posology (Dosage): Safe, Toxic, Lethal, Minimum, Maximum doses.
Questioned Document Examination
Definitions & Classifications:
- Document: Any material containing marks, symbols, or signs conveying a message or meaning.
- Legal Sense: Any written statement establishing a right or extinguishing an obligation.
- Supreme Court Rulings (Non-Documents): Draft municipal payroll unapproved (People vs. Camacho); Blank unexecuted official forms (People vs. Santiago); Merchandise pamphlets/books (People vs. Agnis).
- 4 Kinds of Documents: Public, Official, Private, Commercial.
- Elements: Author, Addressee, Date/Age, Writing material, Writing instrument, Content.
Writing Materials & Inks:
- Materials: Papyrus (Cyperus papyrus plant), Parchment (animal skins: sheep/goats, developed in Pergamum), Vellum (fine calfskin).
- Ink History & Types:
- Indian Ink: Oldest ink; amorphous carbon / lamp-black + hide glue.
- Logwood Inks: Obsolete; aqueous extract of logwood + potassium chromate.
- Iron Gallotannate Inks: Used for over 1000 years; fermented gall nuts + iron salts black ferric tannate.
- Alkaline Inks: Quick drying; pH .
- Ink Analysis: U.S. Secret Service library contains ink formulas; analyzed via Thin Layer Chromatography (TLC) and spectrophotometry.
- Writing Instruments History:
- Reed Pens: Frayed ends; used in Near East.
- Quill Pens: Bird wing feathers (goose, swan, crow, turkey); referenced by St. Isidore of Seville.
- Steel Point Pens: Patented 1803 by Bryan Donkin.
- Fountain Pen: Patented 1884 by Lewis Waterman (capillary action feed).
- Ballpoint Pen: Patented 1888 by John Loud.
- Fiber Tip Pen: Invented 1962 by Yukio Horie (Japan).
Questioned vs. Disputed Documents:
- Questioned Document: Any document whose authenticity, origin, or contents are under suspicion or in doubt.
- Disputed Document: Document that is the subject of an active legal contest or argument. (All disputed documents are questioned documents, but not all questioned documents are disputed).
- Anachronism: An error in chronology; matching paper, ink, or writing materials to an incompatible date.
Historical Pioneers & Landmark Cases:
- François Demelle (1609): Published the first treatise on systematic document examination.
- Gilbert Thompson (1882): U.S. Geological Survey; first recorded use of thumbprints on pay chits to prevent document forgery.
- Albert Sherman Osborn: "Father of Questioned Document Examination". Author of Questioned Documents. Key expert witness in the 1932 State vs. Bruno Richard Hauptmann case (Lindbergh kidnapping ransom notes).

- Alfred Dreyfus Case (1894, France): Treason trial involving forged military letters (Bordereau).
Graphology vs. Questioned Document Examination:
- Graphology: Pseudoscience attempting to profile personality traits from handwriting features.

- Forensic Questioned Document Examiner: Scientific analysis of physical document details to determine authorship, source, or authenticity.
Handwriting Mechanics & Terminology:
- Muscles Involved: Extensor muscles (push pen up for upward strokes) and Flexor muscles (pull pen down for downward strokes).
- Styles: Cursive (connected letters), Script (separated letters), Block (capitalized).
- Movements: Finger, Hand (wrist pivot), Forearm (elbow support), Whole Arm (blackboard motion).
- Stroke Terminology:
- Arc: Top internal curve inside loops ().
- Ascender: Upper loop extending above top ratio line ().
- Descender: Lower loop extending below baseline ().
- Baseline: Ruled or imaginary line upon which writing rests.
- Beard / Embellishment: Rudimentary initial upstroke.
- Bowl: Fully rounded circular form ().
- Diacritics: Elements added to complete letters (-dots, -crosses).
- Hiatus / Pen Jump: Gap occurring in a continuous stroke without lifting pen.
- Pen Lift: Interruption caused by lifting pen from paper.
- Retracing: Superimposing a stroke over an existing stroke.
- Patching / Retouching: Careful correction of a defective stroke portion (common forgery defect).
Handwriting Characteristics:
- Class Characteristics: General features common to a specific school or system of writing (e.g., Palmer, Zaner-Bloser, Spencerian).
- Individual Characteristics: Unique habits resulting from personal muscular control, speed, slant, proportion, alignment, skill level, and pressure.
- Types of Standards / Exemplars:
- Collected Standards: Authentic signatures/writings executed in the ordinary course of business/life (preferably written within of disputed date).
- Request Standards: Exemplars written upon dictation by the subject for comparison.
- Post Litem Motam Exemplars: Writings produced after the dispute arose (disqualified due to self-serving bias).
Signatures & Forgery Classifications:
- Signature Types: Formal/Conventional, Informal/Cursory (Semi-personalized or Personalized), Careless Scribble.
- Forgery Classes:
- Simple / Spurious Forgery: Forger writes name without attempting to imitate the genuine style.
- Simulated Forgery: Freehand drawing imitating a genuine signature model in front of the forger.
- Traced Forgery: Transferring an exact facsimile of a genuine signature via Carbon Outline, Indention, Transmitted Light, or Laser methods.
- Cut and Paste Forgery: Assembled using photocopies or digital scanners.
Alterations, Erasures, & Typewriting:
- Alterations: Additions, Deletions, Interlineation (writing between lines), Intercallation (substituting whole pages).
- Erasures: Mechanical (abrasive scraping) vs. Chemical (ink eradicators/bleaching agents).
- Charred Documents: Deciphered using Infra-Red photography/imaging.
- Typewriting Identification:
- Pica: .
- Elite: .
- Teletype: .
- Defects: Alignment defects (twisted, off-its-feet, horizontal/vertical mis-alignment), Clogged typefaces.
Banknote Counterfeiting & Philippine Currency (NGC):
- Printing Processes: Engraving (intaglio raised ink on genuine notes), Letterpress (serial numbers), Offset Printing (planographic, common for counterfeiters).
- Philippine New Generation Currency (NGC):
- 4 Adjectives: Matibay (Abaca blend), Makulay, Maganda, Malinis.
- Tactile Marks (Elderly/Visually Impaired): Pairs of horizontal bands (1000-Piso = 5 pairs; 500-Piso = 4 pairs; 200-Piso = 3 pairs; 100-Piso = 2 pairs; 50-Piso = 1 pair).
- Key Features:
- 1000-Piso: Blue; Tubbataha Reefs, South Sea Pearl; Abad Santos, Lim, Escoda.
- 500-Piso: Yellow; Underground River, Blue-Naped Parrot; Corazon & Benigno Aquino Jr.
- 200-Piso: Green; Chocolate Hills, Tarsier; Diosdado Macapagal.
- 100-Piso: Violet; Mayon Volcano, Whale Shark (Butanding); Manuel Roxas.
- 50-Piso: Red; Taal Lake, Giant Trevally (Maliputo); Sergio Osmeña.
- 20-Piso: Orange; Banaue Rice Terraces, Palm Civet; Manuel L. Quezon.
Lie Detection Techniques (Polygraphy)
Definitions & Core Concepts:
- Polygraphy: Scientific method of detecting deception using a polygraph instrument.
- Polygraph: Instrument measuring and recording physiological responses (blood pressure, pulse, respiration, electrodermal resistance) during questioning.
- Stimulus: Force or motion exciting sensory receptors.
- Response: Activity resulting from stimulation. Specific Response is any deviation from normal baseline tracings.
- Deception: Act of deceiving or misleading, usually accompanied by lying.
Major Sections & Components of a Polygraph Instrument:
- Pneumograph Component: Records breathing/respiration cycles via two corrugated rubber tubes attached around the chest (Thoracic/Pneumo 1) and abdomen (Abdominal/Pneumo 2).
- Galvanograph Component: Measures electrodermal activity (EDA / Galvanic Skin Response [GSR]) via finger electrode plates attached to the index and ring fingers.
- Cardiosphygmograph Component: Measures cardiovascular activity via a Blood Pressure Cuff over the brachial artery, inflated using a pump bulb to a baseline measured on a Sphygmomanometer (typically ). Records relative blood volume (systolic and diastolic pressures), pulse rate, pulse amplitude, and the dicrotic notch.
- Kymograph Component: Paper feed mechanism driving chart paper at a constant uniform speed of ( = ).
- Pen and Inking System: Capillary ink pens recording permanent tracings on the moving chart.
Tripod Foundation / Premises of Polygraphy:
- Mechanical Leg Premise: Machine is capable of graphically recording reliable physiological changes.
- Physiological Leg Premise: Stimulation of the autonomic nervous system causes involuntary, diagnosable physiological changes.
- Psychological Leg Premise: Involuntary mental and emotional processes of an individual consciously attempting deception trigger autonomic responses when something is at stake.
Psychophysiology of Lying & Autonomic Nervous System:
- Central Nervous System (CNS): Brain and spinal cord; controls voluntary motor/sensory functions.
- Autonomic Nervous System (ANS): Controls involuntary physiological functions. Divided into two complementary branches:
- Sympathetic Nervous System (SNS): Emergency/action system ("Fight, Flight, or Freeze" response). Secretes Epinephrine (Adrenaline) and Norepinephrine (Noradrenaline) from the adrenal glands, elevating blood pressure, heart rate, and skin conductivity while suppressing digestion.
- Parasympathetic Nervous System (PNS): Restorative/braking system; maintains internal balance (Homeostasis).
Non-Polygraph Deception Detection Methods:
- Ancient Ordeals:
- Trial by Combat: Accuser and accused fight; loser judged guilty.
- Red Hot Iron Ordeal: Licking red hot iron 9 times; burned tongue indicates guilt.
- Boiling Water Ordeal: Plunging arm into boiling water; blisters indicate guilt.
- Ordeal of Rice Chewing: Chewing sathee rice; dry mouth/blood-stained saliva indicates guilt due to salivary inhibition under fear.
- Red Water / Sassy Bark Ordeal: Ingesting toxic red fluid; vomiting clears suspect.
- Donkey's Tail Ordeal: Psychological trick; soot-covered tail in a dark room; suspect who does not touch tail due to fear of braying is caught with clean hands.
- Ordeal of the Bier: Suspect touches corpse; re-bleeding wounds indicate murderer.
- Observation Methods:
- Verbal Clues: Response latency, repeating questions, fragmented sentences, unnecessary oaths, excessive politeness.
- Non-Verbal Clues: Emblems, manipulators (hair pulling, fidgeting), dry mouth (frequent swallowing, Adam's apple movement), pupil dilation, altered blink rate.
- Hypnotism: Introduced 1778 by Franz Anton Mesmer. Inadmissible in court due to lack of general scientific acceptance, distortion of facts, suggestibility, and memory fabrication.
- Pharmacological Methods (Inadmissible):
- Truth Serum: Hyoscine Hydrobromide administered hypodermically.
- Narcoanalysis / Narcosynthesis: Intravenous Sodium Amytal or Sodium Pentothal.
- Intoxication: Alcohol ("In vino veritas").
- Other Instrumentation:
- Word Association Test: Sir Francis Galton (1879); measures response time latency.
- Psychological Stress Evaluator (PSE): Voice stress analysis measuring inaudible laryngeal micro-tremors (invented by Allan Bell Jr. and Charles McQuinston).
- Brain Wave Fingerprinting: EEG measuring the brain wave triggered by familiar crime details (invented by Dr. Lawrence Farwell).
- Pupillometry / EyeTracking / EyeDetect: Assessing pupil dilation and eye movement.
- Ancient Ordeals:
History & Pioneers of Polygraphy:
- Daniel Defoe (1730): Suggested taking pulse rate to detect lying.
- Angelo Mosso (1878): Used plethysmograph and scientific cradle to study fear and cardiovascular changes.
- Sir James Mackenzie (1892/1906): Constructed clinical ink polygraph.
- Cesare Lombroso (1895): First to use an instrument (Hydrosphygmograph) in actual criminal interrogations.
- Vittorio Benussi (1914): Discovered respiration changes (inhalation/exhalation ratio) accompanying deception via pneumograph.
- Dr. William Moulton Marston (1915/1917): Developed discontinuous systolic blood pressure test using a sphygmomanometer; self-proclaimed "father of the polygraph".
- Dr. John A. Larson (1921): Built the first continuous lie detector recording blood pressure, pulse, and respiration simultaneously. The real "Father of Lie Detection".
- Leonarde Keeler (1926/1949): Designed metal recording bellows, built-in kymograph, added galvanograph, developed Relevant-Irrelevant technique. "Father of Modern Polygraphy".
- Cleve Backster (1960s): Formulated Backster Zone Comparison Technique, Psychological Set Theory, Anticlimax Dampening Concept, and Color Coding (Green = Control; Red = Relevant; Black = Symptomatic).
Four Phases of Polygraph Examination:
- Phase I (Preliminary Preparation / Case Review): Reviewing investigation facts, spot reports, and evidence details with the investigator.
- Phase II (Pre-Test Interview): Informing subject of rights, obtaining signed voluntary consent, taking personal data, reviewing test questions.
- Phase III (Instrumental Examination / Actual Test): Attaching sensors (Pneumograph Cardiosphygmograph Galvanograph); running test charts ( minimum, max per chart, max duration per chart, rest between charts).
- Phase IV (Post-Test Interview / Interrogation): Interviewing non-deceptive subjects or interrogating deceptive subjects to elicit a confession.
Types of Test Questions:
- Irrelevant Questions: Neutral baseline questions (e.g., "Do you drink water?").
- Strong Relevant Questions: Direct questions about the offense (e.g., "Did you steal the watch?").
- Weak / Secondary Relevant Questions: Questions probing guilty knowledge or partial involvement.
- Control Questions: Primary Control (known lie, e.g., "Before age 25, did you ever steal anything?") vs. Secondary Control.
- Knowledge Questions: Probing knowledge of identity/facts.
- Guilt-Complex Questions: Based on a fictitious crime to test over-reactivity.
- Symptomatic Questions: Detects outside issues/fears (e.g., "Do you understand I won't ask trick questions?").
- Sacrifice / DYAT Question: "Do you intend to answer truthfully?"
- S-K-Y Questions: Suspect, Know, You questions used in Backster Chart 3.
Chart Markings & Symbols:
- Primary Markings: (Test start), (Test end), ( pressure / galvo amplifier), (Question stimulus start/stop), (Yes answer), (No answer).
- Secondary Markings: (Adjustment), (Talked), (Repeat request), (Cough), (Noise), (Sigh), (Paper jam), (Sniff), (Movement), (Clearing throat).
Diagnostic Errors & Legal Status:
- False Positive: Erroneous decision that a truthful subject is deceptive.
- False Negative: Erroneous decision that a deceptive subject is truthful.
- Inconclusive: Insufficient physiological response variation to make a definitive diagnosis.
- U.S. Benchmark Case: United States v. Frye (1923) $ ightarrow$ Established the Frye Standard; polygraph test results excluded due to lack of general scientific acceptance.
- Philippine Jurisprudence on Polygraph Evidence:
- People v. Daniel (1978): Polygraph test results ruled unreliable.
- People v. Adoviso (1999): Polygraph results rejected; lacking scientific acceptance.
- People v. Reanzares (2000): Lie detector test has never been accepted in Philippine jurisdiction.
- People v. Carpo (2001): Polygraph results are inadmissible as evidence in court.