Biology Lecture Notes: Cellular Transport and Metabolism

Molecular Biology Overview

Biomolecules

  • Sugars: Key role in energy storage and structure.

  • Fatty Acids: Composed of long hydrocarbon chains, essential for energy storage and membrane structure.

Molecular Structures

  • H₂O: Water is crucial for biological reactions and structure.

  • Iomolecu: Related to molecular interactions within biological systems.

Biological Hierarchies

  • Primary Structure: Linear chain of amino acids in a protein.

  • Secondary Structure: Hydrogen bonds form alpha helices and beta sheets.

  • Tertiary Structure: Three-dimensional folding of a protein.

  • Quaternary Structure: Assembly of multiple polypeptide chains into a functional protein.

Membrane Architecture

  • Phospholipids: Form the fundamental bilayer of cell membranes with hydrophilic heads and hydrophobic tails.

    • Key functional roles in membrane fluidity and permeability.

  • Proteins: Integral and peripheral proteins serve diverse functions in cellular processes.

    • Integral Proteins: Span the membrane, involved in transport and communication.

    • Peripheral Proteins: Loosely attached to the membrane, serve roles in signaling and maintaining the cell’s shape.

  • Cholesterol: Modulates membrane fluidity and stability in animal cell membranes.

Transport Mechanisms

  • Diffusion: Movement of molecules from high concentration to low concentration.

    • Facilitated Diffusion: Movement of molecules across membranes via specific transport proteins.

    • Stages in Transport: 5 stages identified in detailed processes of molecular movement across membranes.

  • Active Transport: Movement against the concentration gradient, requiring energy (ATP).

Metabolic Pathways

  • Glycolysis: Anaerobic process of converting glucose into pyruvate, yielding energy in the form of ATP.

    • Involves multiple stages and produces reduced cofactors (e.g., NADH) and ATP.

  • Fermentation: Anaerobic process utilizing glycolysis products in energy production.

  • Citric Acid Cycle (Krebs Cycle): Occurs in the mitochondria, oxidizes acetyl-CoA to produce energy carriers (NADH, FADH₂) and CO₂.

    • Chemical reactions include the conversion of acetyl-CoA into citric acid.

  • Electron Transport Chain: Series of protein complexes that transfer electrons and pump protons to create a gradient for ATP synthesis.

    • Molecular formula: O₂ + 4 e− → 2 H₂O in the final electron transfer reaction.

Tonicity and Osmolarity

  • Osmolarity: Measure of solute concentration in a solution.

    • Hypertonic: Higher solute concentration outside the cell causing the cell to lose water.

    • Isotonic: Equal solute concentrations inside and outside the cell, leading to no net water movement.

    • Hypotonic: Lower solute concentration outside the cell, causing the cell to swell as water enters.

Summary of Key Chemical Processes

  • The interconnected pathways of glycolysis, citric acid cycle and oxidative phosphorylation demonstrate the coupling of energy production and usage in biological systems.