Scientific method

The Scientific Method

Chapter 1: Overview of Biology

  • Biology: the study of living organisms, including areas such as ecology, physiology, and anatomy.

The Scientific Method

A logical, step-by-step approach to solving problems through experimentation.

Steps in the Scientific Method:

  1. Observation: Gathering information about the natural world or noticing an event.

  2. Hypothesis: An educated guess based on observations; a proposed explanation or untested idea.

  3. Experimentation: A procedure undertaken to test a hypothesis.

  4. Collection and Interpretation of Data: Data includes any measurements, observations, or information gathered from experiments.

  5. Conclusion: Interpreting data to prove or disprove the hypothesis.

  6. Relating Conclusion to Hypothesis.

  7. Reporting and Publishing Results: Scientists publish results in scientific journals for peer review, allowing others to evaluate and repeat the experiment.

Edward Jenner - Father of Immunology

  1. Observation: Smallpox was rare among those who had been exposed to cowpox.

  2. Hypothesis: Exposure to cowpox provides immunity to smallpox.

  3. Investigation: Jenner injected an 8-year-old boy with cowpox pus, and after recovery, with smallpox pus; the boy did not develop smallpox.

  4. Conclusion: Infection with cowpox prevents smallpox infection.

  5. Hypothesis Supported: His findings supported the hypothesis.

Theory vs. Principle

  • Theory: A hypothesis that has been proven through repeated experiments (e.g., Theory of Evolution).

  • Principle or Law: Developed from a theory that remains valid over time (e.g., Principle of Gravity, Principle of Friction).

Principles of Experimentation

  • Careful Planning and Design: Only one variable should be changed at a time (independent variable), with remaining factors controlled (controlled variables).

  • Control: A standard for comparison.

  • Safety Considerations: Experiments must be safe.

  • Fairness: Ensuring fair experiments.

Sample Size and Selection

  • Larger sample sizes reduce the risk of results being due to individual differences.

  • Random Selection: Participants chosen by chance eliminates bias.

  • Replicates: Repeating experiments to validate results.

  • Double-Blind Testing: Neither the tester nor the participant knows who is receiving the placebo, eliminating bias.

Limitations of the Scientific Method

  1. Extent of Knowledge: Hypotheses may be wrong without sufficient knowledge.

  2. Basis of Investigation: Poor instrumentation can hinder discovery (e.g., the cell).

  3. Interpretation of Results: Limited knowledge affects result interpretation.

  4. Changes in Natural World: Evolution and environmental adaptations affect organisms.

  5. Accidental Discoveries: Discoveries, like antibiotics by Alexander Fleming, occur by chance.

Possible Sources of Errors in Experiments

  • Too small sample size.

  • Changing multiple variables.

  • Non-random sample selection.

Scientific Method Questions

  1. What is a hypothesis?

  2. What might a hypothesis develop into?

  3. Why is a control important in an experiment?

  4. Explain the purpose of a control in a scientific experiment.

  5. Explain each of the following in relation to the scientific method: hypothesis, control, data, replicate, theory.

  6. As a result of her observations, a scientist may formulate a _____, and then devise a series of _____ to analyze the resulting _____.

  7. Why is a control especially important in biological investigations?

  8. Suggest a suitable control when determining the effect of a herbicide on weed growth.

  9. What are replicates in scientific research?

  10. Where may a scientist publish her results?

  11. Why is a control used when carrying out an experiment?

  12. Why is it a good idea to repeat experiments?

  13. Why are controls important in experiments?

  14. In relation to the scientific method, explain the terms: (i) Experiment and (ii) Theory.

  15. Discuss the effect of a certain mineral on the growth of wheat based on scientific methods and lab procedures.