Origin of Life Notes

Evidence for early life and diversification

  • Life on Earth has existed for around 3.5×1093.5 \times 10^9 years, during which time it has diversified.
  • Existing cells are products of evolution.
  • Early cells may have used RNA as genetic information; ribozymes likely played a role.
  • Prokaryotic cells existed before eukaryotic cells (fossil evidence: stromatolites as ancient microbial mats).
  • Most existing eukaryotic cells likely arose via endosymbiotic events.

RNA World and ribozymes

  • RNA world hypothesis: RNA was the primary carrier of genetic information; ribozymes assisted copying and protection of RNA.
  • DNA later replaced RNA as the genetic material because it is more stable.
  • Proteins replaced many ribozymes due to superior catalytic properties.
  • Ribozymes are RNA molecules that catalyse reactions; ribosomes catalyse peptide bond formation via RNA; ribosomal RNA is catalytic, protein part is largely structural.

LUCA (Last Universal Common Ancestor)

  • LUCA refers to the most recent common ancestor of all life today, not the first life.
  • All existing life shares a common ancestral population.

Origins and early environments

  • Deep-sea hydrothermal vents are candidate origins: warm, hydrogen-rich fluids provide energy and minerals for early metabolism.
  • The Miller–Urey experiment (1953) simulated early Earth conditions (H2O, CH4, NH3, H2) and produced 13/2013/20 amino acids, demonstrating abiotic synthesis is plausible.
  • Stromatolites: oldest fossil evidence of life (~3.7×1093.7 \times 10^9$) years ago; stromatolites still form today in places like Shark Bay and the Bahamas.
  • Oxygenation event: after ~2×1092 \times 10^9 years, cyanobacteria-like organisms produced oxygen via oxygenic photosynthesis; atmospheric O2 rose to 0.45%0.45\%, enabling ozone formation and the eventual rise of eukaryotes, while causing extinctions of many anaerobes.

Protocells and membranes

  • Lipids can spontaneously form micelles and vesicles (amphipathic nature: hydrophilic heads outward, hydrophobic tails inward).
  • Compartments within protocells allow accumulation of reactants and selective chemistry, a step toward cellular life.

Endosymbiotic theory and eukaryotic evolution

  • Protists are among the oldest primitive eukaryotes; main groups include Protozoa and Algae.
  • Lynn Margulis proposed endosymbiotic theory: chloroplasts and mitochondria originated as free-living prokaryotes engulfed by a host cell and retained in a symbiotic relationship.
  • Evidence supporting endosymbiosis:
    • Inner membranes resemble prokaryotic membranes more than those of the host.
    • Mitochondria and chloroplasts divide by binary fission like prokaryotes.
    • They contain circular DNA and their own ribosomes, which are more prokaryote-like than eukaryotic.
    • They rely on nuclear-encoded proteins for many functions, indicating integration with the host.

Key takeaway points for quick recall

  • Life began at least 3.5×1093.5 \times 10^9 years ago; stromatolites provide early fossil evidence.
  • RNA may have been the first genetic material; ribozymes could catalyse essential reactions.
  • LUCA is the common ancestral node of all current life, not the first life.
  • Endosymbiosis explains why mitochondria and chloroplasts have prokaryote-like features.
  • Energy-rich environments like hydrothermal vents and abiotic synthesis experiments (Miller–Urey) support possible pathways for the origin of life.