MICROBIOLOGY

Upper respiratory system



Nose, Pharynx, Middle ear and Eustachian tube, Nasolacriminal duct

 Lower respiratory system

Larynx, Trachea, Bronchial tubes

Trachea and bronchial tubes contain ciliated mucous and prevents microorganisms from reaching the lungs by moving them up to the throat



Alveoli (1 cell thick sacs where gases are exchanged)

If microorganisms reach the lungs alveolar macrophages phagocytize them

Pleura (a double layered membrane that surrounds the lungs)

Normal microbiota of the lungs

Upper respiratory tract

The pathogenic microbes are prevented from growing too large due to competition from non pathogenic microbes

Lower respiratory microbes:

Cilia sends microbes to the throat

Bacterial Diseases of the Upper Respiratory Tract 

Sore throat, Scarlet fever, Diphtheria, Otitis Media

Sore throat

subacute

Streptococcal pharyngitis (strep throat)

Caused by Streptococcal pyogenes which also causes impetigo, erysipelas and acute bacterial endocarditis

S. pyogenes is able to resist phagocytosis

It releases an enzyme called streptokinase which can lyse fibrin clots (this stops blood from clotting)

It also releases streptolysins which are cytotoxic to tissue cells, RBC's, and leukocytes

Not all sore throats are caused by S. pyogenes they can be caused by other bacteria and viruses Symptoms: Local inflammation, fever, pain in the throat May cause tonsillitis, Swollen lymph nodes

Spread by respiratory secretions

Scarlet fever

Occurs when a bacteriophage has injected its genetic material into a S. pyogenes bacterium

This causes the S. pyogenes to release an erythrogenic toxin

Erythrogenic toxins produces redness of the tongue as well has a rash on the skin

The tongue eventually loses its upper membrane and the tongue becomes red and large

The tongue may also look like a spotted strawberry

This is a rare disease and goes away on its own Diptheria:

Caused by Corynebacterium diphtheria

Starts with a sore throat and fever, followed by general malaise and swelling of the neck When you get a DTP shot, the D stands for Diptheria Most people have a harmless strain of this in their throat

Characteristic sign of diphtheria is a grayish membrane that forms in the throat in response to the infection

The grayish membrane is made up of fibrin, dead tissue and bacterial cells and can block the air passages so air cannot get to the lungs

Can also release a toxin that gets into the blood and interfere with protein synthesis







It can cause kidney and heart failure or it may affect the nervous system and cause paralysis

Spread by droplet infection

Usually there are 5 or fewer cases per year

When you get a tetanus shot you usually get this in it also.

Cutaneous diphtheria

Usually occurs in American Indians and lower income people

Diphteria enters a wound or similar skin lesion

Causes slow healing skin lesions that are covered by a gray membrane

The toxin does not enter the blood stream

Otitis Media (Ear ache)

An infection of the middle ear

Pathogens in the ear form pus which puts pressure against the eardrum causing it to become swollen and painful

Occurs mainly in young children due to the Eustachian tube which drains the middle ear being horizontal so pathogens don't drain down into the throat

Otitis media can be caused by many different bacteria and viruses

The most common bacteria is S. pyogenes

Antibiotics have been given for years but it is not known if they are affective

Viral diseases of the upper respiratory tract

The common cold

Most common cause is a rhinovirus

Second most common cause is coronavirus

Cold viruses prefer temperatures that are slightly lower, so they infect areas close to openings such as the nose

One rhinovirus is enough to cause a cold

It is not known how the cold virus is spread

Antibiotics are not useful on colds

There is no evidence that cold weather causes colds

If you don't treat a cold it goes away in 7 days, if you do treat it, it goes away in a week Less common in old people because they build up an immunity to it

Symptoms:

Sneezing, runny nose, congestion, usually no fever Bacterial diseases of the lower respiratory tract

Pertussis, Tuberculosis, Bacterial Pneumonias, Melioidosis

Pertussis

The P in DTP vaccine which works until you are about 12 years old

Bordetella pertussis is also known as "whooping cough"

Initial stage:

Catarrhal stage looks like a common cold

The bacteria attaches to the ciliated cells in the trachea where it prevents the cilia from moving then destroys the cell

Paroxysmal stage: This causes mucous to build up which then causes the infected person to struggle to cough up the mucous

A person then goes through a coughing episode which can last up to one minute.

The gasping for air between coughs causes a "Whooping sound"

The coughing can be severe enough to cause broken ribs in young children





Tracheal cytotoxin: damages ciliated cells

Pertussis toxin: Enters the bloodstream and causes systemic problems

Tuberculosis

Convalescence stage: Lasts for months and ends with the person getting better

Caused by Mycobacterium tuberculosis

Usually caught by being inhaled

If it makes it all the way down to the alveoli it is usually phagocytized by a macrophage in the alveoli If some of the of the tuberculosis survives inside the macrophage it starts to reproduce

This causes the macrophage to release chemicals that signal other macrophages to come to the area The extra macrophages form a layer around the tuberculosis bacteria (an early tubercle) and release chemicals (enzymes and cytokines) that cause a lung-damaging inflammation

A few weeks later the macrophages die and symptoms appear.

The tubercle bacilli are released forming a caseous center in tubercle.

Caseous means the dead macrophages appear as a soft, white dead cell mass The tuberculin bacterium remains latent at this stage The dead mass of macrophages eventually becomes calcified

The caseous center enlarges and starts to turn to liquid in a process called liquefaction

Eventually the tubercle breaks open and the TB enters the blood stream where it can go and affect other organs

Pneumonia

Pneumonia is an inflammation of the lung

There are several types of pneumonia

Most pneumonia are caused by bacteria

The most common bacterial pneumonia is Streptococcus pneumonia and is therefore aka typical pneumonia

Atypical pneumonia has slower onset, less fever and chest pain

Other pneumonias can be caused by viruses, fungi, protozoans are called "atypical pneumonia"

Pneumonias can also be named after the area of the lung they infect

Lobar pneumonia only affects a single lobe of the lung, usually caused by S. pneumonia

Bronchopneumonia: the alveoli next to a bronchi is affected

Pleurisy is caused by different pneumonias and the pleural membranes are infected

Pneumococcal Pneumonia

Caused by S. pneumoniae which also causes otitis media, meningitis and sepsis Affected the bronchi and alveoli

The cells pair together and form a capsule around it so it can resist phagocytosis

Symptoms are high fever, difficulty breathing and chest pain

The lungs appear red due to dilation of blood vessels

The alveoli fill with red blood cells, neutrophils and fluid from surrounding tissues

A rust colored sputum (fluid from the lungs that can be spat out) is coughed up from the lungs

There may also not be any symptoms present and many healthy people may carry it

How bad someone is affected by pneumococcal pneumonia depends on how well they are able to fight off infections

Haemophilus influenzae Pneumonia

Caused by Haemophilus influenzae

Similar to Pneumococcal pneumonia

Usually affects people who are alcoholics, poor nutrition, cancer or diabetes





Mycoplasmal pneumonia (walking pneumonia)

Caused by mycoplasmal pneumonia

It is a bacterial pneumonia that acts like an atypical pneumonia

Symptoms are a low grade fever cough and headache

Legionellosis (Legionaires' Disease)

Caused by Legionella pneumonia which can replicate in macrophages

Symptoms: High fever, cough,

Usually affects: Men over 50, especially heavy smokers and drinkers It cannot be transferred from person to person

The microbe can grow in the water air conditioning cooling systems

Recent outbreaks have been traced to whirlpool spas, humidifiers, showers, and potting soil it has also been found in the water lines of many hospitals due to the low temperatures of hot water in the hospital. Hospitals have put in copper-silver ionization systems to resist this It is also able to resist low levels of chlorine in water It exists primarily in biofilms that are highly protective Pontiac Fever

Also caused by Legionnaire's disease

Symptoms are usually fever, muscle aches, and a cough Self limiting

Psittacosis (Ornithosis)

Caused by Chlamydophila psittaci

First found in psittacine bird such as parakeets and other parrots

It is now known that it can be found from other birds such as pigeons, chickens, ducks and turkeys They form "elementary bodies" which are resistant to environmental stress so the Psittacosis can be transferred through the air.

It is not transferred from human to human,

Usually humans get it by breathing in the dust from droppings of fowl

The birds usually have diarrhea, ruffled feathers, respiratory illnesses, and a droopy appearance Many birds carry the pathogen in their spleen and don't have symptoms unless stressed Pet store employees and people involved in raising turkeys are at the greatest risk of disease Symptoms:

Fever, headache, chills are symptoms.

A person may not even know they have it but then become stressed and makes symptoms appear

If disorientation and delirium occurs it means the nervous system has been affected

Chlamydial Pneumonia

A pneumonia that was first found in college students

Similar to the walking pneumonia

May be responsible for atherosclerosis which is the deposition of fatty deposits that block arteries Transmitted person to person by the respiratory route

Half the U.S. population has antibodies to it meaning it is a common infection

Q Fever

Caused by Coxiella burnetii

Has a wide range of symptoms but 60% of cases have no symptoms at all

In acute cases it can cause high fever, headaches, muscle aches and coughing as well as chronic fatigue for months

Affects the heart 2% of the time





in chronic Q-fever it may cause endocarditis, sometimes up to 5 years after the initial infection. If there were no symptoms in the acute stage then the association with Q fever may be missed It is transmitted from animals such goats, sheep and cattle as well as domesticated animals by ticks is found on the placenta of calves

Humans get it by drinking unpasteurized milk and by breathing it in while a cow is given birth because it Inhaling a single pathogen is enough to cause infection

Melioidosis

Found mainly in moist soil in southeast Asia and northern Australia Affects immunocompromised people, especially diabetics

May appear as an abscess on body tissues that resembles necrotizing fasciitis

Death occurs due to septic shock

Transmission is by inhalation or through puncture wounds and ingestion

Viral Diseases of the Lower Respiratory System

Viral pneumonia

Respiratory Syncytial Virus (RSV)

Influenza (Flu)

Viral pneumonia

Can occur as a complication of influenza, measles or the chickenpox Respiratory Syncytial Virus (RSV)

The most common cause of viral respiratory disease in infants between 2-6 months old

About 4500 infant's die/year due to RSV

It can also be life threatening in older adults and is commonly misdiagnosed as influenza

Usually occurs in the winter or early spring

The virus causes cells to fuse together and form a syncytium which is a large multinucleated cell

Symptoms are coughing and wheezing that last for more than a week

Spread by droplet infection

Most of the time it goes away on its own

Influenza (Flu)

Symptoms are fever, chills, headache, a muscle aches Recovery occurs in a few days

Kills up to 50,000 Americans per year

Influenza Virus

Made up of 8 separate RNA strands of different lengths enclosed by a layer outer protein coat (capsid) which is surrounded by a lipid bilayer called an envelope

In the bilayer there are spikes; one spike is called a hemagglutinin (HA) spikes are sharp and neuraminidase (NA) spikes which look like a golf ball on a tee

There are about 500 HA spikes which allow the virus to recognize and attach to body cells before infecting them

Antibodies are directed at the HA spikes

The hemagglutinin spikes cause blood to clump together when the virus is mixed with blood

There are about 100 NA spikes on a virus

They are responsible for helping the virus break loose after it has infected a cell

Fungal Infection of the Lower Respiratory System

Fungi produce spores that are spread through the air

Some of these can get into the lower respiratory tract

Due to an increase in the number of immunosuppressed people, the amount of people with LRT fungal infections is growing

The fungal infections of the lower respiratory system are








Histoplasmosis, Coccidioidomycosis, Pneumocystis Pneumonia, Blastomycosis

Histoplasmosis

Caused by Histoplasma capsulatum

Found mainly in states along the Ohio and Mississippi rivers where 75% of the population has antibodies to it

In places like main it is extremely uncommon

Starts off in the lungs and may spread to the blood and lymph and cause lesions in almost all the organs of the body

On X-rays it looks similar to tuberculosis

Most of the time there are only mild symptoms

The Histoplamsa does not live in birds but feeds off of their poop It can be found in bats due to their lower body temperature

Humans get it from by breathing it in Coccidioidomycosis

Caused by Coccidioides immitis

Found in Alkaline soils in the Southwest

In California it is known as Valley Fever or San Joaquin Fever

A person can get this by just driving through an area

Most infections are asymptomatic and most people recover within a week

About 50 people die a year from this

Pneumocystis Pneumonia

Caused by Pneumocystis jirovecii

Affects the alveoli

Scientists are not sure if it is a fungus or a protozoan

People who are immunocomprised have symptoms but healthy people usually don't

Immunocomprised people who have Pneumocystis may be the reservoir because it is not found in the environment, animals, or healthy adults

Blastomycosis

Caused by the fungus Blastomyces dermatitidis

Found in the soil in the Mississippi and Ohio River valleys

Kills about 50 people per year

Usually asymptomatic

Begins in the lungs where it resembles pneumonia

Cutaneous ulcers can appear

There may be abscesses and tissue destruction that can lead to significant complications if left untreated. If the infection spreads, it can result in systemic symptoms such as fever, chills, and weight loss, indicating a more severe progression of the disease.