Protist Lab

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  • Protists are an extremely diverse assortment of eukaryotes

  • Protists are more diverse than all other eukaryotes

  • Protists are no longer classified in a single kingdom, they are polyphyletic

  • Advances in eukaryotic systematics have caused the classification of protists to change significantly

  • Using clades is a more valuable approach

    • A clade is defined as a group of species, whose members are descendants from a common ancestral species- exemplifying one phylogenetic group.

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  • Protists are very nutritionally diverse

  • Some Protists are autotrophs, which contain chloroplasts and can synthesize their own food through photosynthesis (i.e. algae)

  • Some Protists are heterotrophs, obtain their food by consuming other organisms

  • Some Protista such as protozoans, get their food through phagocytosis (similar to animals)

  • Some protista such as slime molds, get their food by absorption (similar to fungi)

  • Mixotrophs, which combine photosynthesis and heterotrophic nutrition

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  • Protists are also diverse in habitat, including freshwater and marine species

  • Reproduction and life cycles are also highly varied among protists, with both sexual and asexual species

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  • Protists are currently classified into 4 supergroups: Excavata, "SAR" Clade, Archaeplastida, Unikonta

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  • Protista Clades

    • Euglenozoa (Supergroup: Excavata)- example Trypanosome

    • Alveolata (Supergroup SAR)- example Paramecia

    • Stramenopila (Supergroup SAR)- examples Diatoms, Brown Algae

    • Rhizarians (Supergroup SAR)- examples Foraminiferans & Radiolarians

    • Amoebozoans (Supergroup Unikonta)- examples Amoeba, Physarum

    • Chlorophyta (Supergroup Archaeplastida)- examples Green algae- Spirogyra, Ulva

    • Rhodophyta (Supergroup Archaeplastida)-- example red algae

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  • Euglenozoans have flagella with a unique internal structure

  • Euglenozoa is a diverse clade that includes:

    • Predatory heterotrophs

    • Photosynthetic autotrophs

    • Free living or pathogenic parasites

  • Their main feature distinguishing them as a clade is a spiral or crystalline rod of unknown function inside their flagella

  • They can have one or more flagella

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  • Euglenozoa: Trypanosoma levisi

  • Trypanosoma levisi is a parasite found in the blood of rats and is transmitted by fleas

  • These trypanosomes are long and thin, with a flagellum that runs from the anterior end and twists back

  • The flagellum is joined to the complete length of the cell via an undulating membrane

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  • Alveolates have sacs beneath the plasma membrane

  • They can be autotrophs or heterotrophs

  • Members of the clade Alveolata have membrane-bound sacs (alveoli) just under the plasma membrane

  • The function of these alveoli is unknown but researchers think that they may help stabilize the cell surface or regulate the cells water and ion content

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  • Alveolata: Paramecium caudatum

  • They are commonly found in freshwater habitats

  • Ciliates, a large varied group of protists, are named for their use of cilia to move and feed

  • The cilia may completely cover their cell surface or be clustered in a few rows or tufts

  • They have large macronuclei and small micronuclei

  • They can have one or more of each type of these nuclei

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  • Stramenopila

  • The clade Stramenopila includes several groups of heterotrophs as well as certain groups of algae

  • They are mostly photosynthetic

  • Name of the clade is derived from the Latin for Stramen-straw and pilos- hair) which refers to a flagellum with numerous fine hairlike projections

  • Most have a “hairy” flagellum paired with a “smooth” flagellum

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  • Stramenopila: diatoms

  • They can be unicellular or form aggregates

  • Diatoms are unicellular algae with a unique two-part, glass-like wall of hydrated silica embedded in an organic matrix

  • The walls consist of two parts- valves that overlap like a petri dish the base and its lid

  • These walls provide protection from the crushing jaws of predators

Page 28: Brown Algae n Stramenopila

  • Brown algae are the longest, fastest-growing algae

  • They are photosynthetic and multicellular

  • Their life cycle has alternating generations

  • They are brown in color due to the carotenoid pigment- fucoxanthin

  • They can attach to rocks by a holdfast

  • Some brown algae have specialized organs like stem-like stipe and leaf-like blades, and gas-filled bladders

Page 30: Rhizarians- Foraminiferans and Radiolarans

  • Foraminiferans and Radiolarans are solitary amoeboid and heterotrophic organisms

  • They contain threadlike pseudopodia

  • Foraminiferans have porous shells called tests

  • Foraminiferans derive nourishment from symbiotic algae

  • They can be marine planktonic or benthic

Page 36: Radiolarians

  • Radiolarians are marine planktonic organisms

  • They have tests made of silica

  • Radiolarians have needle-like pseudopodia supported by microtubules

  • They phagocytose microorganisms with their pseudopodia

Page 40: Ameobozoa: Amoeba

  • Amoeba are solitary and live in freshwater or marine habitats

  • They are unicellular and have an irregular body shape

  • They move using pseudopods

  • They lack shells

Page 45: Chlorophyta (Green Algae)

  • Most chlorophytes live in fresh water, although many are marine

  • They are photosynthetic autotrophs and contain chlorophylls a and b

  • They can be unicellular, form aggregates, be filamentous, be colonial, or be multicellular

  • They have many characteristics in common with land plants

Page 49: Red Algae (Rhodophyta)

  • Rhodophyta are photoautotrophs

  • They have several pigments including chlorophyll a, phycocyanin, and phycoerythrin

  • They can be single-celled or multicellular