Protist Lab
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Protists are an extremely diverse assortment of eukaryotes
Protists are more diverse than all other eukaryotes
Protists are no longer classified in a single kingdom, they are polyphyletic
Advances in eukaryotic systematics have caused the classification of protists to change significantly
Using clades is a more valuable approach
A clade is defined as a group of species, whose members are descendants from a common ancestral species- exemplifying one phylogenetic group.
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Protists are very nutritionally diverse
Some Protists are autotrophs, which contain chloroplasts and can synthesize their own food through photosynthesis (i.e. algae)
Some Protists are heterotrophs, obtain their food by consuming other organisms
Some Protista such as protozoans, get their food through phagocytosis (similar to animals)
Some protista such as slime molds, get their food by absorption (similar to fungi)
Mixotrophs, which combine photosynthesis and heterotrophic nutrition
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Protists are also diverse in habitat, including freshwater and marine species
Reproduction and life cycles are also highly varied among protists, with both sexual and asexual species
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Protists are currently classified into 4 supergroups: Excavata, "SAR" Clade, Archaeplastida, Unikonta
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Protista Clades
Euglenozoa (Supergroup: Excavata)- example Trypanosome
Alveolata (Supergroup SAR)- example Paramecia
Stramenopila (Supergroup SAR)- examples Diatoms, Brown Algae
Rhizarians (Supergroup SAR)- examples Foraminiferans & Radiolarians
Amoebozoans (Supergroup Unikonta)- examples Amoeba, Physarum
Chlorophyta (Supergroup Archaeplastida)- examples Green algae- Spirogyra, Ulva
Rhodophyta (Supergroup Archaeplastida)-- example red algae
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Euglenozoans have flagella with a unique internal structure
Euglenozoa is a diverse clade that includes:
Predatory heterotrophs
Photosynthetic autotrophs
Free living or pathogenic parasites
Their main feature distinguishing them as a clade is a spiral or crystalline rod of unknown function inside their flagella
They can have one or more flagella
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Euglenozoa: Trypanosoma levisi
Trypanosoma levisi is a parasite found in the blood of rats and is transmitted by fleas
These trypanosomes are long and thin, with a flagellum that runs from the anterior end and twists back
The flagellum is joined to the complete length of the cell via an undulating membrane
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Alveolates have sacs beneath the plasma membrane
They can be autotrophs or heterotrophs
Members of the clade Alveolata have membrane-bound sacs (alveoli) just under the plasma membrane
The function of these alveoli is unknown but researchers think that they may help stabilize the cell surface or regulate the cells water and ion content
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Alveolata: Paramecium caudatum
They are commonly found in freshwater habitats
Ciliates, a large varied group of protists, are named for their use of cilia to move and feed
The cilia may completely cover their cell surface or be clustered in a few rows or tufts
They have large macronuclei and small micronuclei
They can have one or more of each type of these nuclei
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Stramenopila
The clade Stramenopila includes several groups of heterotrophs as well as certain groups of algae
They are mostly photosynthetic
Name of the clade is derived from the Latin for Stramen-straw and pilos- hair) which refers to a flagellum with numerous fine hairlike projections
Most have a “hairy” flagellum paired with a “smooth” flagellum
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Stramenopila: diatoms
They can be unicellular or form aggregates
Diatoms are unicellular algae with a unique two-part, glass-like wall of hydrated silica embedded in an organic matrix
The walls consist of two parts- valves that overlap like a petri dish the base and its lid
These walls provide protection from the crushing jaws of predators
Page 28: Brown Algae n Stramenopila
Brown algae are the longest, fastest-growing algae
They are photosynthetic and multicellular
Their life cycle has alternating generations
They are brown in color due to the carotenoid pigment- fucoxanthin
They can attach to rocks by a holdfast
Some brown algae have specialized organs like stem-like stipe and leaf-like blades, and gas-filled bladders
Page 30: Rhizarians- Foraminiferans and Radiolarans
Foraminiferans and Radiolarans are solitary amoeboid and heterotrophic organisms
They contain threadlike pseudopodia
Foraminiferans have porous shells called tests
Foraminiferans derive nourishment from symbiotic algae
They can be marine planktonic or benthic
Page 36: Radiolarians
Radiolarians are marine planktonic organisms
They have tests made of silica
Radiolarians have needle-like pseudopodia supported by microtubules
They phagocytose microorganisms with their pseudopodia
Page 40: Ameobozoa: Amoeba
Amoeba are solitary and live in freshwater or marine habitats
They are unicellular and have an irregular body shape
They move using pseudopods
They lack shells
Page 45: Chlorophyta (Green Algae)
Most chlorophytes live in fresh water, although many are marine
They are photosynthetic autotrophs and contain chlorophylls a and b
They can be unicellular, form aggregates, be filamentous, be colonial, or be multicellular
They have many characteristics in common with land plants
Page 49: Red Algae (Rhodophyta)
Rhodophyta are photoautotrophs
They have several pigments including chlorophyll a, phycocyanin, and phycoerythrin
They can be single-celled or multicellular