Chapter 4: Behavioral Genetics

Lecture 4: Behavioral Genetics

Trovell Crith


Chapter 4: Key Concepts

  • 4.1 Behaviors vary in their heritability

  • 4.2 Behavioral variation is associated with genetic variation

  • 4.3 The environment influences behavior via gene expression

  • 4.4 Genomic approaches correlate gene expression with behavioral phenotypes

  • 4.5 Genes can limit behavioral flexibility


4.1 Behaviors Vary in Their Heritability

Key Definitions

  • Observed Traits (Phenotype, P): The physical or behavioral characteristics of an individual.

  • Genotype (G): The genetic makeup of an individual, encompassing all its genetic material.

  • Behavioral Genetics: The study of how genes and the environment contribute to individual differences in behavior.

Behavioral Equation

  • Behavior (P) is influenced by:

    • Genotype (G)

    • Environment (E)

    • Gene-environment Interactions (GXE)

  • Contributions:

    • Phenotypic Variation (VP) = Genetic Variation (VG) + Environmental Variation (VE)

Heritability

  • Heritability (h^2): The proportion of phenotypic variation within a trait that is attributable to genetic influences.

    • Broad-sense heritability: Includes all genetic effects that influence phenotype.

Genetic Effects of Alleles

  • Additive Effects (A): Average effect of alleles on the phenotype.

  • Dominance Effects (D): Interactions between alleles at a single locus.

  • Epistasis (I): Interactions between genes at different loci.

  • Genetic Variation Equation:

    • VG = VA + VD + VI

Narrow-Sense Heritability

  • Higher narrow-sense heritability increases the likelihood that parent and offspring will share traits.

  • This concept is assessed through the similarity of behavior between parents and offspring, often visualized through a Parent/Offspring Regression plot.

    • Greater slope: Indicates strong resemblance between offspring and parents.

    • Lower slope: Suggests that phenotypic variance is not due to genetic variance.

Research Example in Dog Behavior

  • Research Question: Are key behaviors for service and hunting dogs heritable? (Ruefenacht et al. 2002)

  • Methods: Analyzed 25 years of behavioral field test results for German shepherds of known pedigree.

Results
  • Heritability scores for traits like gunshot reaction ranged from 0.09 to 0.24.

  • Conclusion: Behavioral traits in dogs have genetic and environmental components.


4.2 Behavioral Variation and Genetic Variation

Instinctual Behaviors

  • Search for genetic basis of behaviors:

    • Behaviors that are instinctive are performed the same way each time, expressed the first time, and observed even in isolated individuals (e.g., blinking).

Types of Instinctual Behavior
  • Reflexes: Automatic movement in response to stimuli.

  • Fixed Action Patterns: Behaviors with minimal variation that occur in sequence and conclude without interruption (Lorenz and Tinbergen, 1957).

Research on Fruit Fly Behavior

  • Observation by Bastock (1956): Yellow mutants of fruit flies are less common in nature than in lab settings.

  • Research Question: Why are yellow forms of fruit flies rarer in nature?

  • Hypothesis: The mutation leads to behavioral changes that result in low reproductive success in natural populations.

Methods
  • Subjects: Fruit flies (Drosophila melanogaster).

  • Process: Interbred wild-type and mutant yellow flies to create inbred flies and conducted mating trials.

Results
  • Wild-type males showed higher mating success than yellow mutants.

  • Yellow flies engaged in less courtship behavior.

  • Conclusion: Genotype variations lead to variations in phenotypic traits impacting reproductive success.

Major and Minor Genes

  • Major Gene: Responsible for a significant portion of phenotypic variation.

  • Minor Gene: Contributes small amounts to variation in phenotype.

Genetic Interactions
  • Epistasis: Interaction of genes at distinct loci.

  • Pleiotropy: A single gene affects multiple traits (e.g., "yellow" gene influencing color and courtship traits).

Fire Ant Research

  • Observation: Variance in social structures of fire ant colonies (monogyne vs polygyne).

  • Research Question: Do genes influence social organization in fire ants? (Ross, 1997)

Methods
  • Study Type: Collected samples from monogyne and polygyne colonies.

  • Techniques: Conducted starch gel electrophoresis to compare gene frequencies.

Results
  • Notable allelic frequency changes at the Gp-9 locus.

  • Conclusion: Specific alleles regulate social structures in fire ants.


4.3 Environmental Influence on Gene Expression

Gene Expression Processes

  • Genes encode for molecules though they do not produce actions directly; they influence brain functionality.

  • Environmental factors notably affect gene expression.

  • Gene Expression: The process of forming gene products influenced by environmental inputs.

Research on Zebrafish Aggression
  • Research Question: How does varying environments impact aggression in zebrafish?

Hypothesis
  • Both developmental environment (DE) and behavior environment (BE) affect aggression.

Predictions
  • If DE affects behavior only, aggression is highest in normoxic (high-oxygen) water.

  • If DE and BE interact, aggression will be highest where matched.

Methods
  • Process: Collected eggs from full-sibling clutches, raised them in normoxic and hypoxic conditions, and tested adult aggression levels.

Results
  • Fish showed aggression levels akin to their developmental environment.

  • Conclusion: Significant interaction exists between DE and BE affecting aggression levels.

Social Environment and Birdsong Development

  • Bird calls (like songs) are innate among all birds in the order Passeriformes.

  • Sensitive Learners: Must experience tutor songs shortly after hatching; otherwise learning is impaired.

  • Open-ended Learners: Can acquire new song features throughout life.

Research on FoxP2 Gene
  • Research Question: What role does the FoxP2 gene play in song development? (Haesler et al. 2007)

  • Methods: Used viral techniques to knockdown FoxP2 expression in zebra finches and monitored song recordings.

Results
  • Knockdown birds had reduced expression of FoxP2 compared to controls.

  • Omitted specific song elements, implicating FoxP2’s essential role in song learning.


4.4 Genomic Approaches Correlate Gene Expression with Behavior

Observation in Fish Mating Tactics

  • Dominant males defend territories, while sneaker males display differing tactics.

  • Research Question: Do the behavioral and morphological differences correlate with gene expression?

  • Featured Research: Black-faced blennies (Tripterygion delaisi).

Methods
  • Examined gene expression during mating season differing across reproductive roles.

Results
  • Territorial males showed increased expression of certain genes than sneaker males/females.

  • Conclusion: Male phenotype plasticity is more strongly linked with gene expression than morphological features.


4.5 Genes Can Limit Behavioral Flexibility

Concept of Animal Personalities

  • Animal Personalities: Consistent variations in behaviors within a population over time or across circumstances (e.g., bold vs shy; aggressive vs docile).

Research on Great Tits Behavior
  • Research Question: Is exploratory behavior in great tits genetic?

  • Hypothesis: Believed exploratory behavior has heritable components.

Methods
  • Recorded movements in controlled settings with multiple layers and treatments.

Results
  • Found significant correlation between parental and offspring exploratory behaviors.

  • Concluded exploratory behavior in great tits has heritable traits.

Salamander Personalities

  • Research Question: How do salamander larvae react to predation risk?

  • Hypothesis: Activity levels will vary based on perceived threat.

Results
  • Larvae displayed consistent behaviors across predation risk treatments, illustrating distinct personalities and heritable behavior components.

Environmental Impact on Jumping Spider Personalities

  • Research Question: How does rearing environment affect exploratory behavior?

  • Methods: Varied rearing conditions for jumping spiders and assessed exploratory behavior in open-field tests.

  • Results: Environment significantly affected exploratory latency and behavior, suggesting substantial influences on personality.


# Questions?