Protists Notes

Overview: Living Small

  • Even a low-power microscope can reveal a great variety of organisms in a drop of pond water.
  • "Protist" is the informal name of the kingdom of mostly unicellular eukaryotes, but there are some colonial and multicellular species.
  • Protists constitute a paraphyletic group, and Protista is no longer valid as a kingdom.
  • Protists exhibit more structural and functional diversity than any other group of eukaryotes.

Protist Nutrition and Reproduction

  • Protists can reproduce asexually or sexually, or by the sexual processes of meiosis and syngamy.
  • Protists, the most nutritionally diverse of all eukaryotes, include:
    • Photoautotrophs - contain chloroplasts.
    • Heterotrophs - absorb organic molecules or ingest larger food particles.
    • Mixotrophs - combine photosynthesis and heterotrophic nutrition.

Endosymbiosis in Eukaryotic Evolution

  • There is considerable evidence that much protist diversity has its origins in endosymbiosis.
  • Mitochondria evolved by endosymbiosis of an aerobic prokaryote.
  • Plastids evolved by endosymbiosis of a photosynthetic cyanobacterium.
  • Primary endosymbiosis:
    • Heterotrophic eukaryote engulfs cyanobacterium.
    • Over the course of evolution, this membrane was lost.
  • Secondary endosymbiosis:
    • Red alga or green alga engulfed by another eukaryote.
    • Examples: Dinoflagellates, Apicomplexans, Stramenopiles, Euglenids, Chlorarachniophytes.

Protist Diversity

  • Major groups:
    • Excavata: Diplomonads, Parabasalids, Euglenozoans.
    • Chromalveolata: Alveolates (Dinoflagellates, Apicomplexans, Ciliates), Stramenopiles (Diatoms, Golden algae, Brown algae, Oomycetes).
    • Rhizaria: Chlorarachniophytes, Forams, Radiolarians.
    • Archaeplastida: Red algae, Chlorophytes, Charophyceans, Land plants.
    • Unikonta: Slime molds, Gymnamoebas, Entamoebas, Nucleariids, Fungi, Choanoflagellates, Animals.

Examples of Protists

  • Diplomonads:
    • Intestinal parasites.
  • Diatoms:
    • Unicellular algae.
    • Cell walls are glass-like and made of silica.
  • Forams:
    • Have porous shells called tests.
  • Volvox:
    • Colonial freshwater chlorophytes.
  • Amoeba:
    • Have pseudopods for ingestion and motility.
  • Trichomonas vaginalis:
    • Parabasalids that commonly inhabit the female vagina.
    • Has an undulating membrane.

Euglenozoans

  • A diverse clade that includes predatory heterotrophs, photosynthetic autotrophs, and pathogenic parasites.
  • Distinguished by a spiral or crystalline rod of unknown function inside their flagella.
  • Includes the kinetoplastids and euglenids.
  • Crystalline rod inside flagella.
  • Trypannnosoma:
    • The Kinetoplastid that causes Sleeping Sickness.

Euglenids

  • Can be both autotrophic and heterotrophic.
    • Adaptations for photosynthesis: eyespot, light detector, chloroplast.
    • Contractile vacuole for osmoregulation.
    • Pellicle: Protein bands beneath plasma membrane provide strength and flexibility.

Dinoflagellates

  • Some are bioluminescent; Some cause "Red Tides".
    • Alveoli: sacs under the plasma membrane.

Apicomplexans

  • Plasmodium:
    • Causes malaria.
    • Two host life cycle: mosquito and human.
    • Stages: sporozoites, merozoites, gametocytes, zygote.

Ciliates

  • A large varied group of protists, are named for their use of cilia to move and feed.
  • Have large macronuclei and small micronuclei.
    • Micronuclei function during conjugation, a sexual process that produces genetic variation.
  • Conjugation is separate from reproduction, which generally occurs by binary fission.
  • Paramecium:
    • Feeding, waste removal, and water balance via contractile vacuoles, oral groove, cell mouth, cilia, food vacuoles, macronucleus, and micronucleus.

Diatoms

  • Unicellular algae with a unique two-part, glass-like wall of hydrated silica.
  • Usually reproduce asexually, and occasionally sexually.
  • A major component of phytoplankton and are highly diverse.
  • Fossilized diatom walls compose much of the sediments known as diatomaceous earth.

Golden Algae

  • Named for their color, which results from yellow and brown carotenoids.
  • The cells of golden algae are typically biflagellated, with both flagella near one end.
  • All golden algae are photosynthetic, and some are also heterotrophic.
  • Most are unicellular, but some are colonial.

Brown Algae

  • The largest and most complex algae. All are multicellular, and most are marine.
  • Include many species commonly called “seaweeds.” Giant seaweeds called kelps live in deep parts of the ocean.
  • Have the most complex multicellular anatomy of all algae.
  • The algal body is plantlike but lacks true roots, stems, and leaves and is called a thallus.
  • The rootlike holdfast anchors the stemlike stipe, which in turn supports the leaflike blades.

Alternation of Generations

  • A variety of life cycles have evolved among the multicellular algae.
  • The most complex life cycles include an alternation of generations, the alternation of multicellular haploid (gametophyte) and diploid forms (sporophyte).
  • Heteromorphic generations are structurally different.
  • Isomorphic generations look similar.
  • Brown Algae Alternation of Generations.

Oomycetes (Water Molds and Their Relatives)

  • Include water molds, white rusts, and downy mildews… Fungus-like.
  • They were once considered fungi based on morphological studies.
  • Most oomycetes are decomposers or parasites.
  • They have filaments (hyphae) that facilitate nutrient uptake.
  • Their ecological impact can be great, as in Phytophthora infestans causing potato blight.
  • Life Cycle of Oomycetes = Water Mold: Asexual and sexual reproduction.

Rhizarians

  • Defined by DNA similarities: DNA evidence supports Rhizaria as a monophyletic clade.
  • Amoebas move and feed by pseudopodia; some but not all belong to the clade Rhizaria.
  • Rhizarians include forams and radiolarians.

Forams and Radiolarians

  • Foraminiferans, or forams, are named for porous, generally multichambered shells, called tests. Pseudopodia extend through the pores in the test.
  • Foram tests in marine sediments form an extensive fossil record.
  • Radiolarians have tests fused into one delicate piece, made of silica.
  • Radiolarians use their pseudopodia to engulf microorganisms by phagocytosis.

Red and Green Algae

  • The closest relatives of land plants
  • Over a billion years ago, a heterotrophic protist acquired a cyanobacterial endosymbiont.
  • The photosynthetic descendants of this ancient protist evolved into red algae and green algae.
  • Land plants are descended from the green algae.
  • Archaeplastida is a supergroup used by some scientists and includes red algae, green algae, and land plants.

Red Algae

  • Reddish in color due to an accessory pigment call phycoerythrin, which masks the green of chlorophyll.
  • The color varies from greenish-red in shallow water to dark red or almost black in deep water. Red algae are the most abundant large algae in coastal waters of the tropics.
  • Red algae are usually multicellular; the largest are seaweeds.
  • Examples: Bonnemaisonia hamifera, Dulse (Palmaria palmata), Nori (Porphyra).

Green Algae

  • Named for their grass-green chloroplasts.
  • Plants are descended from the green algae.
  • The two main groups are chlorophytes and charophyceans.
  • Most chlorophytes live in fresh water, although many are marine. Other chlorophytes live in damp soil, as symbionts in lichens, or in snow.
  • Chlorophytes include unicellular, colonial, and multicellular forms.
  • Examples: Ulva (sea lettuce), Caulerpa.
  • Most chlorophytes have complex life cycles with both sexual and asexual reproductive stages.

Unikonts

  • Include protists that are closely related to fungi and animals
  • The supergroup Unikonta includes animals, fungi, and some protists.
  • This group includes two clades: the amoebozoans and the opisthokonts (animals, fungi, and related protists)
  • The root of the eukaryotic tree remains controversial.

Amoebozoans

  • Amoeba that have lobe- or tube-shaped, rather than threadlike, pseudopodia.
  • They include gymnamoebas, entamoebas, and slime molds.

Slime Molds

  • Slime molds, or mycetozoans, were once thought to be fungi. Molecular systematics places slime molds in the clade Amoebozoa.
  • Many species of plasmodial slime molds are brightly pigmented, usually yellow or orange.
  • At one point in the life cycle, plasmodial slime molds form a mass called a plasmodium (not to be confused with malarial Plasmodium).
  • The plasmodium is undivided by membranes and contains many diploid nuclei. It extends pseudopodia through decomposing material, engulfing food by phagocytosis.
  • Life Cycle of Plasmodial Slime Mold.

Cellular Slime Molds

  • Form multicellular aggregates in which cells are separated by their membranes.
  • Cells feed individually, but can aggregate to form a fruiting body.
  • Dictyostelium discoideum is an experimental model for studying the evolution of multicellularity.
  • Life Cycle of Cellular Slime Mold: Asexual and sexual reproduction.

Protists in Ecological Relationships

  • Protists are found in diverse aquatic environments.
  • Protists often play the role of symbiont or producer.
  • Some protist symbionts ++ benefit their hosts
    • Dinoflagellates nourish coral polyps that build reefs.
    • Hypermastigotes digest cellulose in the gut of termites.
  • Some protists are parasitic + - symbionts
    • Plasmodium causes malaria.
    • Pfesteria shumwayae is a dinoflagellate that causes fish kills… toxic red tides ..
    • Phytophthora ramorum causes sudden oak death.

Photosynthetic Protists

  • Many protists are important producers that obtain energy from the sun.
  • In aquatic environments, photosynthetic protists and prokaryotes are the main producers.
  • The availability of nutrients can affect the concentration of protists.