Synaptic Transmission and Neural Integration
Synapses
- Functional association of a neuron with another neuron or effector organs (muscle, gland).
- Two types: Electrical and Chemical.
Electrical Synapses
- Direct transfer of Action Potential (AP).
- Neurons linked by gap junctions.
- Functions:
- Rapid communication.
- Synchronous activity.
- Bidirectional communication.
- Both excitation or inhibition at the same synapse.
- Identified in retina, cortex, brainstem, hypothalamus.
Chemical Synapses
- Functional Anatomy: Presynaptic neuron, synaptic cleft (30-50
\text{ nm} wide), postsynaptic neuron. - Unidirectional communication.
- Locations: Axodendritic, Axosomatic, Axoaxonic, Dendrodendritic.
- Presynaptic Axon Terminal: Neurotransmitter-containing vesicles, voltage-gated Ca^{2+} channels, reuptake molecules.
- Postsynaptic Neuron: Receptors, enzymes.
Synaptic Communication Steps (Chemical)
- Action potential arrives at presynaptic terminal.
- Voltage-gated Ca^{2+} channels open.
- Ca^{2+} entry triggers vesicle docking and neurotransmitter secretion via exocytosis.
- Neurotransmitter diffuses across synaptic cleft and binds to receptors on postsynaptic membrane.
- Response in postsynaptic cell (e.g., change in membrane potential).
- Neurotransmitter removed from cleft by: enzymatic degradation, reuptake into presynaptic terminal (degraded or recycled), or diffusion out of cleft.
Synaptic Delay
- 0.5-5 \text{ msec} delay between AP arrival and postsynaptic V_m change.
- Caused by: Ca^{2+} entry, vesicle docking, and neurotransmitter release.
Signal Transduction at Chemical Synapses
- Channel-linked (Ionotropic) Receptors:
- Ligand-gated ion channels.
- Fast change in postsynaptic V_m (Postsynaptic Potential - PSP).
- Channel closes quickly as neurotransmitter dissociates.
- G protein-coupled (Metabotropic) Receptors:
- Slow acting.
- Involve direct coupling or second messenger pathways.
Postsynaptic Potentials (PSPs)
- Graded potential in postsynaptic cell due to receptor-neurotransmitter binding.
- Excitatory Postsynaptic Potential (EPSP): Causes depolarization.
- Inhibitory Postsynaptic Potential (IPSP): Causes hyperpolarization or membrane stabilization.
Excitatory Postsynaptic Potential (EPSP)
- Fast Response:
- Cation channels (e.g., Na^{+} and K^{+}) open.
- At resting Vm = -70 \text{ mV}, Na^{+} has a higher electrochemical gradient (relative to E{Na} = +60 \text{ mV} and E_K = -94 \text{ mV}).
- Short duration, few to hundreds of msec.
- Slow Response:
- Activation of metabotropic receptor with G protein and cAMP second messenger.
- K^{+} channels close, reducing K^{+} leak out.
- Na^{+} still leaks in, leads to depolarization.
- Longer duration, seconds to hours.
Inhibitory Postsynaptic Potential (IPSP)
- Neurotransmitter binds, opening channels for K^{+} or Cl^{-}.
- If K^{+} channels open: K^{+} moves out, causing hyperpolarization.
- If Cl^{-} channels open:
- Cl^{-} moves in, causing hyperpolarization (in cells with Cl^{-} ATPase pump which maintains an inward Cl^{-} gradient).
- Cl^{-} stabilizes membrane potential (in cells with Cl^{-} leak channels and no Cl^{-} ATPase pump, where Cl^{-} is at equilibrium).
- Can also stabilize membrane if both Cl^{-} and excitatory (cation) channels open, balancing ion movements.
- IPSPs are graded potentials: Higher AP frequency \rightarrow more neurotransmitter \rightarrow greater ion permeability change \rightarrow greater hyperpolarization.