Everything in Cells

Magnification & Resolution

Magnification

• Magnification is how many times a larger an image is than the object.

• magnification =image size/object size

  • Image size is how large the object appears when you view it through a microscope

  • Object size is the real size of the object

• Resolution

Resolution is the ability to distinguish between two separate points

The higher the resolution the clearer the image is.

Calibrating a microscope

• The specimen’s length/width can be measured using an eyepiece graticule (which is placed in a eyepiece)

Calibrating an eyepiece graticule

1. Fix the stage micrometre into place on the stage

2. Look though the eyepiece to line up the micrometre and the graticule

3. Count the number of graticule divisions that fit into one micrometre division

4. Calculate the size of each graticule division ta that magnification:

   • Graticule division = size of one micrometer division / no.of graticule divisions

Resolution

Light Microscopes

Using a light microscope

1. Clip the prepared microscope slide onto the stage

2. Sleect the objective lens with the lowest power

3. Use the coarse focus to bring the stage just below he objective lens

4. Use